Experiment: Paraquat dichloride 0.032 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_23405 and AO353_23410 are separated by 69 nucleotides AO353_23410 and AO353_23415 are separated by 276 nucleotides AO353_23415 and AO353_23420 are separated by 10 nucleotides AO353_23420 and AO353_23425 are separated by 37 nucleotides
AO353_23405: AO353_23405 - hypothetical protein, at 5,129,162 to 5,129,362
_23405
AO353_23410: AO353_23410 - hypothetical protein, at 5,129,432 to 5,129,716
_23410
AO353_23415: AO353_23415 - lysine transporter LysE, at 5,129,993 to 5,130,580
_23415
AO353_23420: AO353_23420 - AraC family transcriptional regulator, at 5,130,591 to 5,131,424
_23420
AO353_23425: AO353_23425 - 3-demethylubiquinone-9 3-methyltransferase, at 5,131,462 to 5,131,965
_23425
Position (kb) 5129
5130
5131 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5129.003 kb on - strand at 5129.036 kb on + strand at 5129.081 kb on + strand at 5129.081 kb on + strand at 5129.082 kb on - strand at 5129.092 kb on + strand at 5129.093 kb on - strand at 5129.105 kb on + strand at 5129.105 kb on + strand at 5129.106 kb on - strand at 5129.106 kb on - strand at 5129.278 kb on + strand, within AO353_23405 at 5129.333 kb on + strand, within AO353_23405 at 5129.334 kb on - strand, within AO353_23405 at 5129.334 kb on - strand, within AO353_23405 at 5129.334 kb on - strand, within AO353_23405 at 5129.334 kb on - strand, within AO353_23405 at 5129.384 kb on + strand at 5129.433 kb on + strand at 5129.433 kb on + strand at 5129.439 kb on + strand at 5129.440 kb on - strand at 5129.440 kb on - strand at 5129.549 kb on - strand, within AO353_23410 at 5129.650 kb on + strand, within AO353_23410 at 5129.650 kb on + strand, within AO353_23410 at 5129.651 kb on - strand, within AO353_23410 at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.702 kb on - strand at 5129.702 kb on - strand at 5129.702 kb on - strand at 5129.849 kb on + strand at 5129.849 kb on + strand at 5129.852 kb on + strand at 5129.852 kb on + strand at 5129.852 kb on + strand at 5129.852 kb on + strand at 5129.852 kb on + strand at 5129.853 kb on - strand at 5129.853 kb on - strand at 5129.853 kb on - strand at 5129.853 kb on - strand at 5129.853 kb on - strand at 5129.853 kb on - strand at 5129.925 kb on + strand at 5129.925 kb on + strand at 5129.925 kb on + strand at 5129.925 kb on + strand at 5129.925 kb on + strand at 5129.925 kb on + strand at 5129.926 kb on - strand at 5129.933 kb on + strand at 5129.933 kb on + strand at 5129.934 kb on - strand at 5129.934 kb on - strand at 5129.948 kb on - strand at 5129.948 kb on - strand at 5129.975 kb on + strand at 5129.976 kb on - strand at 5129.976 kb on - strand at 5130.114 kb on + strand, within AO353_23415 at 5130.114 kb on + strand, within AO353_23415 at 5130.115 kb on - strand, within AO353_23415 at 5130.115 kb on - strand, within AO353_23415 at 5130.115 kb on - strand, within AO353_23415 at 5130.180 kb on + strand, within AO353_23415 at 5130.314 kb on + strand, within AO353_23415 at 5130.314 kb on + strand, within AO353_23415 at 5130.337 kb on - strand, within AO353_23415 at 5130.337 kb on - strand, within AO353_23415 at 5130.429 kb on - strand, within AO353_23415 at 5130.443 kb on + strand, within AO353_23415 at 5130.444 kb on - strand, within AO353_23415 at 5130.444 kb on - strand, within AO353_23415 at 5130.468 kb on - strand, within AO353_23415 at 5130.491 kb on + strand, within AO353_23415 at 5130.524 kb on - strand at 5130.578 kb on + strand at 5130.578 kb on + strand at 5130.578 kb on + strand at 5130.578 kb on + strand at 5130.579 kb on - strand at 5130.868 kb on + strand, within AO353_23420 at 5130.869 kb on - strand, within AO353_23420 at 5130.869 kb on - strand, within AO353_23420 at 5130.869 kb on - strand, within AO353_23420 at 5130.869 kb on - strand, within AO353_23420 at 5130.869 kb on - strand, within AO353_23420 at 5130.869 kb on - strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.012 kb on + strand, within AO353_23420 at 5131.012 kb on + strand, within AO353_23420 at 5131.012 kb on + strand, within AO353_23420 at 5131.013 kb on - strand, within AO353_23420 at 5131.013 kb on - strand, within AO353_23420 at 5131.013 kb on - strand, within AO353_23420 at 5131.013 kb on - strand, within AO353_23420 at 5131.013 kb on - strand, within AO353_23420 at 5131.013 kb on - strand, within AO353_23420 at 5131.031 kb on + strand, within AO353_23420 at 5131.032 kb on - strand, within AO353_23420 at 5131.130 kb on + strand, within AO353_23420 at 5131.138 kb on + strand, within AO353_23420 at 5131.138 kb on + strand, within AO353_23420 at 5131.138 kb on + strand, within AO353_23420 at 5131.139 kb on - strand, within AO353_23420 at 5131.139 kb on - strand, within AO353_23420 at 5131.255 kb on + strand, within AO353_23420 at 5131.255 kb on + strand, within AO353_23420 at 5131.255 kb on + strand, within AO353_23420 at 5131.255 kb on + strand, within AO353_23420 at 5131.256 kb on - strand, within AO353_23420 at 5131.256 kb on - strand, within AO353_23420 at 5131.256 kb on - strand, within AO353_23420 at 5131.256 kb on - strand, within AO353_23420 at 5131.256 kb on - strand, within AO353_23420 at 5131.316 kb on - strand, within AO353_23420 at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.461 kb on - strand at 5131.461 kb on - strand at 5131.461 kb on - strand at 5131.461 kb on - strand at 5131.461 kb on - strand at 5131.463 kb on + strand at 5131.463 kb on + strand at 5131.463 kb on + strand at 5131.463 kb on + strand at 5131.463 kb on + strand at 5131.464 kb on - strand at 5131.464 kb on - strand at 5131.464 kb on - strand at 5131.464 kb on - strand at 5131.464 kb on - strand at 5131.464 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.032 mg/mL remove 5,129,003 - -0.3 5,129,036 + -0.6 5,129,081 + +0.4 5,129,081 + -1.1 5,129,082 - -0.7 5,129,092 + +1.2 5,129,093 - -0.5 5,129,105 + -0.9 5,129,105 + -0.4 5,129,106 - +0.2 5,129,106 - -1.4 5,129,278 + AO353_23405 0.58 -0.1 5,129,333 + AO353_23405 0.85 -0.3 5,129,334 - AO353_23405 0.86 -1.4 5,129,334 - AO353_23405 0.86 -1.4 5,129,334 - AO353_23405 0.86 -0.8 5,129,334 - AO353_23405 0.86 -0.5 5,129,384 + +0.8 5,129,433 + +0.7 5,129,433 + +1.2 5,129,439 + +1.1 5,129,440 - -1.8 5,129,440 - -0.4 5,129,549 - AO353_23410 0.41 -1.2 5,129,650 + AO353_23410 0.76 +1.2 5,129,650 + AO353_23410 0.76 +0.2 5,129,651 - AO353_23410 0.77 +1.3 5,129,701 + +0.5 5,129,701 + -0.2 5,129,701 + +0.7 5,129,701 + -2.0 5,129,701 + -1.0 5,129,701 + -0.8 5,129,701 + +1.3 5,129,702 - +1.8 5,129,702 - -0.3 5,129,702 - -0.0 5,129,849 + -0.1 5,129,849 + -0.1 5,129,852 + -0.2 5,129,852 + +0.4 5,129,852 + +0.0 5,129,852 + -0.5 5,129,852 + -1.5 5,129,853 - +0.4 5,129,853 - -0.1 5,129,853 - -1.3 5,129,853 - -0.4 5,129,853 - +3.5 5,129,853 - -0.7 5,129,925 + +0.3 5,129,925 + -0.7 5,129,925 + +0.2 5,129,925 + +0.2 5,129,925 + +0.1 5,129,925 + -0.3 5,129,926 - -0.8 5,129,933 + -0.8 5,129,933 + +0.1 5,129,934 - -0.6 5,129,934 - -0.5 5,129,948 - -0.6 5,129,948 - +0.7 5,129,975 + -0.9 5,129,976 - -2.0 5,129,976 - +0.5 5,130,114 + AO353_23415 0.21 -0.6 5,130,114 + AO353_23415 0.21 -0.7 5,130,115 - AO353_23415 0.21 -1.2 5,130,115 - AO353_23415 0.21 -0.3 5,130,115 - AO353_23415 0.21 -0.2 5,130,180 + AO353_23415 0.32 -0.2 5,130,314 + AO353_23415 0.55 +0.5 5,130,314 + AO353_23415 0.55 -1.3 5,130,337 - AO353_23415 0.59 +0.3 5,130,337 - AO353_23415 0.59 -0.7 5,130,429 - AO353_23415 0.74 -0.7 5,130,443 + AO353_23415 0.77 +0.3 5,130,444 - AO353_23415 0.77 -0.3 5,130,444 - AO353_23415 0.77 -0.7 5,130,468 - AO353_23415 0.81 -2.2 5,130,491 + AO353_23415 0.85 -0.7 5,130,524 - -0.3 5,130,578 + -0.4 5,130,578 + +0.0 5,130,578 + -3.1 5,130,578 + -0.1 5,130,579 - +1.1 5,130,868 + AO353_23420 0.33 -1.3 5,130,869 - AO353_23420 0.33 +1.3 5,130,869 - AO353_23420 0.33 +0.1 5,130,869 - AO353_23420 0.33 -1.2 5,130,869 - AO353_23420 0.33 +1.7 5,130,869 - AO353_23420 0.33 +1.7 5,130,869 - AO353_23420 0.33 +1.5 5,131,005 + AO353_23420 0.50 +0.4 5,131,005 + AO353_23420 0.50 -0.1 5,131,005 + AO353_23420 0.50 +1.5 5,131,005 + AO353_23420 0.50 +0.2 5,131,005 + AO353_23420 0.50 +1.2 5,131,005 + AO353_23420 0.50 -0.9 5,131,005 + AO353_23420 0.50 -2.8 5,131,005 + AO353_23420 0.50 -0.6 5,131,006 - AO353_23420 0.50 -1.4 5,131,006 - AO353_23420 0.50 -0.7 5,131,006 - AO353_23420 0.50 +0.7 5,131,006 - AO353_23420 0.50 -0.9 5,131,006 - AO353_23420 0.50 -0.8 5,131,006 - AO353_23420 0.50 -3.0 5,131,006 - AO353_23420 0.50 -0.1 5,131,006 - AO353_23420 0.50 -0.6 5,131,006 - AO353_23420 0.50 -0.1 5,131,006 - AO353_23420 0.50 +0.2 5,131,012 + AO353_23420 0.50 +0.9 5,131,012 + AO353_23420 0.50 -0.3 5,131,012 + AO353_23420 0.50 +0.2 5,131,013 - AO353_23420 0.51 -0.8 5,131,013 - AO353_23420 0.51 -0.1 5,131,013 - AO353_23420 0.51 -0.3 5,131,013 - AO353_23420 0.51 +1.7 5,131,013 - AO353_23420 0.51 -0.5 5,131,013 - AO353_23420 0.51 -1.0 5,131,031 + AO353_23420 0.53 -1.5 5,131,032 - AO353_23420 0.53 +0.2 5,131,130 + AO353_23420 0.65 -0.7 5,131,138 + AO353_23420 0.66 +0.7 5,131,138 + AO353_23420 0.66 -0.6 5,131,138 + AO353_23420 0.66 -0.8 5,131,139 - AO353_23420 0.66 -0.5 5,131,139 - AO353_23420 0.66 -0.7 5,131,255 + AO353_23420 0.80 -1.1 5,131,255 + AO353_23420 0.80 -1.8 5,131,255 + AO353_23420 0.80 +0.5 5,131,255 + AO353_23420 0.80 -1.1 5,131,256 - AO353_23420 0.80 +1.7 5,131,256 - AO353_23420 0.80 -0.2 5,131,256 - AO353_23420 0.80 -0.2 5,131,256 - AO353_23420 0.80 -0.8 5,131,256 - AO353_23420 0.80 -0.0 5,131,316 - AO353_23420 0.87 +0.9 5,131,460 + +1.1 5,131,460 + -0.0 5,131,460 + -0.1 5,131,460 + +1.5 5,131,460 + -1.2 5,131,460 + -1.9 5,131,460 + +0.2 5,131,460 + +0.0 5,131,460 + -0.7 5,131,461 - -0.5 5,131,461 - +0.9 5,131,461 - +1.5 5,131,461 - -0.3 5,131,461 - -0.7 5,131,463 + -1.4 5,131,463 + -0.5 5,131,463 + -0.1 5,131,463 + -0.8 5,131,463 + -1.1 5,131,464 - +0.2 5,131,464 - +0.3 5,131,464 - -0.7 5,131,464 - -1.3 5,131,464 - +0.5 5,131,464 - +0.3
Or see this region's nucleotide sequence