Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS24185 and EX31_RS24190 are separated by 4 nucleotides EX31_RS24190 and EX31_RS24195 are separated by 177 nucleotides EX31_RS24195 and EX31_RS24200 are separated by 124 nucleotides EX31_RS24200 and EX31_RS24205 are separated by 51 nucleotides EX31_RS24205 and EX31_RS24210 are separated by 72 nucleotides
EX31_RS24185: EX31_RS24185 - DNA-binding transcriptional repressor DeoR, at 606,473 to 607,237
_RS24185
EX31_RS24190: EX31_RS24190 - DNA gyrase inhibitor, at 607,242 to 607,718
_RS24190
EX31_RS24195: EX31_RS24195 - membrane protein, at 607,896 to 608,405
_RS24195
EX31_RS24200: EX31_RS24200 - hypothetical protein, at 608,530 to 608,805
_RS24200
EX31_RS24205: EX31_RS24205 - hypothetical protein, at 608,857 to 609,117
_RS24205
EX31_RS24210: EX31_RS24210 - hypothetical protein, at 609,190 to 609,402
_RS24210
Position (kb) 607
608
609 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 606.913 kb on + strand, within EX31_RS24185 at 606.928 kb on + strand, within EX31_RS24185 at 606.934 kb on + strand, within EX31_RS24185 at 607.068 kb on + strand, within EX31_RS24185 at 607.156 kb on + strand, within EX31_RS24185 at 607.157 kb on - strand, within EX31_RS24185 at 607.193 kb on + strand at 607.193 kb on + strand at 607.193 kb on + strand at 607.193 kb on + strand at 607.194 kb on - strand at 607.194 kb on - strand at 607.194 kb on - strand at 607.194 kb on - strand at 607.194 kb on - strand at 607.194 kb on - strand at 607.235 kb on + strand at 607.321 kb on + strand, within EX31_RS24190 at 607.321 kb on + strand, within EX31_RS24190 at 607.321 kb on + strand, within EX31_RS24190 at 607.322 kb on - strand, within EX31_RS24190 at 607.322 kb on - strand, within EX31_RS24190 at 607.355 kb on - strand, within EX31_RS24190 at 607.381 kb on + strand, within EX31_RS24190 at 607.381 kb on + strand, within EX31_RS24190 at 607.381 kb on + strand, within EX31_RS24190 at 607.442 kb on - strand, within EX31_RS24190 at 607.442 kb on - strand, within EX31_RS24190 at 607.701 kb on + strand at 607.701 kb on + strand at 607.701 kb on + strand at 607.702 kb on - strand at 607.707 kb on + strand at 607.707 kb on + strand at 607.707 kb on + strand at 607.707 kb on + strand at 607.708 kb on - strand at 607.708 kb on - strand at 607.708 kb on - strand at 607.708 kb on - strand at 607.708 kb on - strand at 607.708 kb on - strand at 607.786 kb on - strand at 607.828 kb on + strand at 607.830 kb on + strand at 607.851 kb on + strand at 607.851 kb on + strand at 607.852 kb on - strand at 607.852 kb on - strand at 607.855 kb on + strand at 607.855 kb on + strand at 607.855 kb on + strand at 607.869 kb on + strand at 607.869 kb on + strand at 607.869 kb on + strand at 607.870 kb on - strand at 607.870 kb on - strand at 607.871 kb on + strand at 607.871 kb on + strand at 607.871 kb on + strand at 607.872 kb on - strand at 607.872 kb on - strand at 607.872 kb on - strand at 607.872 kb on - strand at 607.890 kb on + strand at 607.890 kb on + strand at 607.890 kb on + strand at 607.891 kb on - strand at 607.891 kb on - strand at 607.891 kb on - strand at 607.903 kb on + strand at 607.904 kb on - strand at 607.915 kb on + strand at 607.916 kb on - strand at 607.964 kb on + strand, within EX31_RS24195 at 607.964 kb on + strand, within EX31_RS24195 at 607.993 kb on + strand, within EX31_RS24195 at 607.993 kb on + strand, within EX31_RS24195 at 607.993 kb on + strand, within EX31_RS24195 at 607.993 kb on + strand, within EX31_RS24195 at 607.994 kb on - strand, within EX31_RS24195 at 608.008 kb on + strand, within EX31_RS24195 at 608.008 kb on + strand, within EX31_RS24195 at 608.008 kb on + strand, within EX31_RS24195 at 608.009 kb on - strand, within EX31_RS24195 at 608.018 kb on + strand, within EX31_RS24195 at 608.031 kb on + strand, within EX31_RS24195 at 608.032 kb on - strand, within EX31_RS24195 at 608.034 kb on - strand, within EX31_RS24195 at 608.034 kb on - strand, within EX31_RS24195 at 608.055 kb on + strand, within EX31_RS24195 at 608.055 kb on + strand, within EX31_RS24195 at 608.055 kb on + strand, within EX31_RS24195 at 608.055 kb on + strand, within EX31_RS24195 at 608.055 kb on + strand, within EX31_RS24195 at 608.056 kb on - strand, within EX31_RS24195 at 608.056 kb on - strand, within EX31_RS24195 at 608.067 kb on + strand, within EX31_RS24195 at 608.067 kb on + strand, within EX31_RS24195 at 608.067 kb on + strand, within EX31_RS24195 at 608.067 kb on + strand, within EX31_RS24195 at 608.067 kb on + strand, within EX31_RS24195 at 608.067 kb on + strand, within EX31_RS24195 at 608.068 kb on - strand, within EX31_RS24195 at 608.068 kb on - strand, within EX31_RS24195 at 608.068 kb on - strand, within EX31_RS24195 at 608.068 kb on - strand, within EX31_RS24195 at 608.068 kb on - strand, within EX31_RS24195 at 608.068 kb on - strand, within EX31_RS24195 at 608.068 kb on - strand, within EX31_RS24195 at 608.069 kb on + strand, within EX31_RS24195 at 608.069 kb on + strand, within EX31_RS24195 at 608.069 kb on + strand, within EX31_RS24195 at 608.069 kb on + strand, within EX31_RS24195 at 608.069 kb on + strand, within EX31_RS24195 at 608.069 kb on + strand, within EX31_RS24195 at 608.070 kb on - strand, within EX31_RS24195 at 608.070 kb on - strand, within EX31_RS24195 at 608.070 kb on - strand, within EX31_RS24195 at 608.070 kb on - strand, within EX31_RS24195 at 608.070 kb on - strand, within EX31_RS24195 at 608.096 kb on + strand, within EX31_RS24195 at 608.097 kb on - strand, within EX31_RS24195 at 608.097 kb on - strand, within EX31_RS24195 at 608.114 kb on + strand, within EX31_RS24195 at 608.114 kb on + strand, within EX31_RS24195 at 608.114 kb on + strand, within EX31_RS24195 at 608.115 kb on - strand, within EX31_RS24195 at 608.142 kb on - strand, within EX31_RS24195 at 608.142 kb on - strand, within EX31_RS24195 at 608.182 kb on + strand, within EX31_RS24195 at 608.182 kb on + strand, within EX31_RS24195 at 608.193 kb on + strand, within EX31_RS24195 at 608.193 kb on + strand, within EX31_RS24195 at 608.193 kb on + strand, within EX31_RS24195 at 608.194 kb on - strand, within EX31_RS24195 at 608.194 kb on - strand, within EX31_RS24195 at 608.194 kb on - strand, within EX31_RS24195 at 608.195 kb on + strand, within EX31_RS24195 at 608.196 kb on - strand, within EX31_RS24195 at 608.248 kb on + strand, within EX31_RS24195 at 608.248 kb on + strand, within EX31_RS24195 at 608.248 kb on + strand, within EX31_RS24195 at 608.248 kb on + strand, within EX31_RS24195 at 608.248 kb on + strand, within EX31_RS24195 at 608.249 kb on - strand, within EX31_RS24195 at 608.249 kb on - strand, within EX31_RS24195 at 608.328 kb on + strand, within EX31_RS24195 at 608.338 kb on - strand, within EX31_RS24195 at 608.372 kb on + strand at 608.373 kb on - strand at 608.392 kb on + strand at 608.392 kb on + strand at 608.393 kb on - strand at 608.393 kb on - strand at 608.404 kb on - strand at 608.412 kb on + strand at 608.459 kb on + strand at 608.526 kb on + strand at 608.526 kb on + strand at 608.526 kb on + strand at 608.526 kb on + strand at 608.526 kb on + strand at 608.526 kb on + strand at 608.526 kb on + strand at 608.527 kb on - strand at 608.602 kb on - strand, within EX31_RS24200 at 608.610 kb on + strand, within EX31_RS24200 at 608.610 kb on + strand, within EX31_RS24200 at 608.610 kb on + strand, within EX31_RS24200 at 608.610 kb on + strand, within EX31_RS24200 at 608.610 kb on + strand, within EX31_RS24200 at 608.611 kb on - strand, within EX31_RS24200 at 608.614 kb on + strand, within EX31_RS24200 at 608.614 kb on + strand, within EX31_RS24200 at 608.633 kb on + strand, within EX31_RS24200 at 608.690 kb on + strand, within EX31_RS24200 at 608.717 kb on + strand, within EX31_RS24200 at 608.718 kb on - strand, within EX31_RS24200 at 608.795 kb on + strand at 608.795 kb on + strand at 608.885 kb on - strand, within EX31_RS24205 at 608.909 kb on + strand, within EX31_RS24205 at 608.910 kb on - strand, within EX31_RS24205 at 608.970 kb on + strand, within EX31_RS24205 at 609.090 kb on + strand, within EX31_RS24205 at 609.115 kb on + strand at 609.115 kb on + strand at 609.115 kb on + strand at 609.132 kb on + strand at 609.149 kb on + strand at 609.149 kb on + strand at 609.268 kb on + strand, within EX31_RS24210
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 606,913 + EX31_RS24185 0.58 -1.2 606,928 + EX31_RS24185 0.59 +0.5 606,934 + EX31_RS24185 0.60 +0.5 607,068 + EX31_RS24185 0.78 +0.2 607,156 + EX31_RS24185 0.89 -0.6 607,157 - EX31_RS24185 0.89 -1.6 607,193 + +0.4 607,193 + +2.6 607,193 + -0.8 607,193 + +0.1 607,194 - +1.6 607,194 - -0.5 607,194 - +0.2 607,194 - -0.8 607,194 - -0.9 607,194 - +0.9 607,235 + +0.3 607,321 + EX31_RS24190 0.17 -0.3 607,321 + EX31_RS24190 0.17 +0.1 607,321 + EX31_RS24190 0.17 -0.1 607,322 - EX31_RS24190 0.17 -0.5 607,322 - EX31_RS24190 0.17 +0.8 607,355 - EX31_RS24190 0.24 -0.0 607,381 + EX31_RS24190 0.29 -0.5 607,381 + EX31_RS24190 0.29 +0.1 607,381 + EX31_RS24190 0.29 -0.1 607,442 - EX31_RS24190 0.42 +1.7 607,442 - EX31_RS24190 0.42 -1.0 607,701 + -0.8 607,701 + +0.2 607,701 + +0.4 607,702 - -0.8 607,707 + -0.3 607,707 + -1.2 607,707 + -0.1 607,707 + +0.3 607,708 - -0.6 607,708 - -1.2 607,708 - +0.0 607,708 - +0.3 607,708 - -0.6 607,708 - -1.1 607,786 - -0.8 607,828 + -1.5 607,830 + +0.4 607,851 + -0.0 607,851 + +1.3 607,852 - -0.6 607,852 - +0.3 607,855 + -1.3 607,855 + -0.7 607,855 + -0.3 607,869 + +0.7 607,869 + +0.1 607,869 + +0.6 607,870 - +0.2 607,870 - -0.9 607,871 + -0.2 607,871 + -0.5 607,871 + -0.3 607,872 - +0.2 607,872 - -1.4 607,872 - -0.8 607,872 - -3.1 607,890 + +0.4 607,890 + +0.3 607,890 + +0.1 607,891 - -1.9 607,891 - +0.5 607,891 - +0.4 607,903 + +0.1 607,904 - +0.6 607,915 + +0.3 607,916 - +1.1 607,964 + EX31_RS24195 0.13 +1.4 607,964 + EX31_RS24195 0.13 -0.4 607,993 + EX31_RS24195 0.19 -1.5 607,993 + EX31_RS24195 0.19 -1.6 607,993 + EX31_RS24195 0.19 -0.2 607,993 + EX31_RS24195 0.19 -1.3 607,994 - EX31_RS24195 0.19 +0.2 608,008 + EX31_RS24195 0.22 +0.1 608,008 + EX31_RS24195 0.22 +0.5 608,008 + EX31_RS24195 0.22 -0.2 608,009 - EX31_RS24195 0.22 +2.6 608,018 + EX31_RS24195 0.24 +0.2 608,031 + EX31_RS24195 0.26 -0.7 608,032 - EX31_RS24195 0.27 +0.6 608,034 - EX31_RS24195 0.27 +1.5 608,034 - EX31_RS24195 0.27 +2.8 608,055 + EX31_RS24195 0.31 +1.0 608,055 + EX31_RS24195 0.31 +0.9 608,055 + EX31_RS24195 0.31 +1.8 608,055 + EX31_RS24195 0.31 +0.7 608,055 + EX31_RS24195 0.31 +0.8 608,056 - EX31_RS24195 0.31 +0.8 608,056 - EX31_RS24195 0.31 -2.3 608,067 + EX31_RS24195 0.34 +0.2 608,067 + EX31_RS24195 0.34 -0.8 608,067 + EX31_RS24195 0.34 +0.5 608,067 + EX31_RS24195 0.34 +0.2 608,067 + EX31_RS24195 0.34 +1.0 608,067 + EX31_RS24195 0.34 -0.8 608,068 - EX31_RS24195 0.34 -1.1 608,068 - EX31_RS24195 0.34 -0.9 608,068 - EX31_RS24195 0.34 +1.8 608,068 - EX31_RS24195 0.34 -0.6 608,068 - EX31_RS24195 0.34 +0.1 608,068 - EX31_RS24195 0.34 +2.1 608,068 - EX31_RS24195 0.34 -0.1 608,069 + EX31_RS24195 0.34 -0.4 608,069 + EX31_RS24195 0.34 +0.7 608,069 + EX31_RS24195 0.34 +1.6 608,069 + EX31_RS24195 0.34 +0.6 608,069 + EX31_RS24195 0.34 +0.3 608,069 + EX31_RS24195 0.34 -1.0 608,070 - EX31_RS24195 0.34 +0.6 608,070 - EX31_RS24195 0.34 -0.1 608,070 - EX31_RS24195 0.34 +0.2 608,070 - EX31_RS24195 0.34 +1.1 608,070 - EX31_RS24195 0.34 -0.6 608,096 + EX31_RS24195 0.39 +0.7 608,097 - EX31_RS24195 0.39 +0.2 608,097 - EX31_RS24195 0.39 -1.3 608,114 + EX31_RS24195 0.43 +0.3 608,114 + EX31_RS24195 0.43 -0.1 608,114 + EX31_RS24195 0.43 -0.3 608,115 - EX31_RS24195 0.43 +1.1 608,142 - EX31_RS24195 0.48 +0.2 608,142 - EX31_RS24195 0.48 +0.8 608,182 + EX31_RS24195 0.56 -0.0 608,182 + EX31_RS24195 0.56 +0.6 608,193 + EX31_RS24195 0.58 +0.2 608,193 + EX31_RS24195 0.58 +0.5 608,193 + EX31_RS24195 0.58 -0.2 608,194 - EX31_RS24195 0.58 -0.8 608,194 - EX31_RS24195 0.58 +1.8 608,194 - EX31_RS24195 0.58 +0.5 608,195 + EX31_RS24195 0.59 +0.2 608,196 - EX31_RS24195 0.59 +0.2 608,248 + EX31_RS24195 0.69 -0.3 608,248 + EX31_RS24195 0.69 +0.0 608,248 + EX31_RS24195 0.69 +0.0 608,248 + EX31_RS24195 0.69 +0.8 608,248 + EX31_RS24195 0.69 -0.4 608,249 - EX31_RS24195 0.69 +1.8 608,249 - EX31_RS24195 0.69 -0.1 608,328 + EX31_RS24195 0.85 -0.5 608,338 - EX31_RS24195 0.87 -1.6 608,372 + -0.1 608,373 - +0.6 608,392 + -1.1 608,392 + -0.0 608,393 - +2.6 608,393 - -0.4 608,404 - -1.6 608,412 + +1.3 608,459 + -0.5 608,526 + -0.6 608,526 + -0.4 608,526 + -0.4 608,526 + -0.5 608,526 + -0.2 608,526 + +0.1 608,526 + -0.2 608,527 - -0.0 608,602 - EX31_RS24200 0.26 +0.5 608,610 + EX31_RS24200 0.29 +0.6 608,610 + EX31_RS24200 0.29 +0.6 608,610 + EX31_RS24200 0.29 -0.2 608,610 + EX31_RS24200 0.29 -1.0 608,610 + EX31_RS24200 0.29 +0.5 608,611 - EX31_RS24200 0.29 -0.3 608,614 + EX31_RS24200 0.30 +2.0 608,614 + EX31_RS24200 0.30 +0.2 608,633 + EX31_RS24200 0.37 -2.6 608,690 + EX31_RS24200 0.58 +0.1 608,717 + EX31_RS24200 0.68 -0.4 608,718 - EX31_RS24200 0.68 -1.6 608,795 + +0.2 608,795 + -1.3 608,885 - EX31_RS24205 0.11 -1.5 608,909 + EX31_RS24205 0.20 -0.2 608,910 - EX31_RS24205 0.20 +1.1 608,970 + EX31_RS24205 0.43 -0.3 609,090 + EX31_RS24205 0.89 -0.4 609,115 + +2.8 609,115 + +0.5 609,115 + -0.8 609,132 + -0.5 609,149 + -0.1 609,149 + +0.4 609,268 + EX31_RS24210 0.37 +0.1
Or see this region's nucleotide sequence