Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23420 and EX31_RS23425 overlap by 7 nucleotides EX31_RS23425 and EX31_RS23430 are separated by 3 nucleotides EX31_RS23430 and EX31_RS23435 are separated by 19 nucleotides
EX31_RS23420: EX31_RS23420 - succinate dehydrogenase cytochrome b556 subunit, at 450,739 to 451,128
_RS23420
EX31_RS23425: EX31_RS23425 - succinate dehydrogenase membrane anchor subunit, at 451,122 to 451,469
_RS23425
EX31_RS23430: EX31_RS23430 - succinate dehydrogenase flavoprotein subunit, at 451,473 to 453,239
_RS23430
EX31_RS23435: EX31_RS23435 - succinate dehydrogenase iron-sulfur subunit, at 453,259 to 453,975
_RS23435
Position (kb) 451
452
453
454 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 450.484 kb on + strand at 450.485 kb on - strand at 450.535 kb on + strand at 450.562 kb on + strand at 450.609 kb on + strand at 450.609 kb on + strand at 450.609 kb on + strand at 450.702 kb on + strand at 450.702 kb on + strand at 450.702 kb on + strand at 450.702 kb on + strand at 450.702 kb on + strand at 450.702 kb on + strand at 450.703 kb on - strand at 450.824 kb on + strand, within EX31_RS23420 at 450.825 kb on - strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.936 kb on + strand, within EX31_RS23420 at 450.936 kb on + strand, within EX31_RS23420 at 450.936 kb on + strand, within EX31_RS23420 at 450.985 kb on + strand, within EX31_RS23420 at 451.004 kb on + strand, within EX31_RS23420 at 451.126 kb on + strand at 451.151 kb on + strand at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.206 kb on + strand, within EX31_RS23425 at 451.208 kb on + strand, within EX31_RS23425 at 451.208 kb on + strand, within EX31_RS23425 at 451.209 kb on - strand, within EX31_RS23425 at 451.217 kb on + strand, within EX31_RS23425 at 451.217 kb on + strand, within EX31_RS23425 at 451.217 kb on + strand, within EX31_RS23425 at 451.297 kb on + strand, within EX31_RS23425 at 451.297 kb on + strand, within EX31_RS23425 at 451.368 kb on + strand, within EX31_RS23425 at 451.431 kb on + strand, within EX31_RS23425 at 451.431 kb on + strand, within EX31_RS23425 at 451.435 kb on + strand at 451.435 kb on + strand at 451.440 kb on + strand at 451.467 kb on + strand at 451.467 kb on + strand at 451.467 kb on + strand at 451.467 kb on + strand at 451.607 kb on + strand at 451.680 kb on + strand, within EX31_RS23430 at 451.680 kb on + strand, within EX31_RS23430 at 451.680 kb on + strand, within EX31_RS23430 at 451.680 kb on + strand, within EX31_RS23430 at 451.680 kb on + strand, within EX31_RS23430 at 451.704 kb on + strand, within EX31_RS23430 at 451.704 kb on + strand, within EX31_RS23430 at 451.704 kb on + strand, within EX31_RS23430 at 451.704 kb on + strand, within EX31_RS23430 at 451.704 kb on + strand, within EX31_RS23430 at 451.705 kb on - strand, within EX31_RS23430 at 451.706 kb on + strand, within EX31_RS23430 at 451.731 kb on + strand, within EX31_RS23430 at 451.731 kb on + strand, within EX31_RS23430 at 451.731 kb on + strand, within EX31_RS23430 at 451.731 kb on + strand, within EX31_RS23430 at 451.731 kb on + strand, within EX31_RS23430 at 451.731 kb on + strand, within EX31_RS23430 at 451.731 kb on + strand, within EX31_RS23430 at 451.732 kb on - strand, within EX31_RS23430 at 451.732 kb on - strand, within EX31_RS23430 at 451.732 kb on - strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.733 kb on + strand, within EX31_RS23430 at 451.815 kb on + strand, within EX31_RS23430 at 451.815 kb on + strand, within EX31_RS23430 at 451.816 kb on - strand, within EX31_RS23430 at 451.871 kb on + strand, within EX31_RS23430 at 451.920 kb on + strand, within EX31_RS23430 at 452.046 kb on + strand, within EX31_RS23430 at 452.123 kb on + strand, within EX31_RS23430 at 452.123 kb on + strand, within EX31_RS23430 at 452.351 kb on + strand, within EX31_RS23430 at 452.429 kb on + strand, within EX31_RS23430 at 452.535 kb on + strand, within EX31_RS23430 at 452.535 kb on + strand, within EX31_RS23430 at 452.535 kb on + strand, within EX31_RS23430 at 452.535 kb on + strand, within EX31_RS23430 at 452.535 kb on + strand, within EX31_RS23430 at 452.535 kb on + strand, within EX31_RS23430 at 452.535 kb on + strand, within EX31_RS23430 at 452.535 kb on + strand, within EX31_RS23430 at 452.535 kb on + strand, within EX31_RS23430 at 452.536 kb on - strand, within EX31_RS23430 at 452.738 kb on + strand, within EX31_RS23430 at 452.788 kb on + strand, within EX31_RS23430 at 452.788 kb on + strand, within EX31_RS23430 at 452.975 kb on + strand, within EX31_RS23430 at 452.975 kb on + strand, within EX31_RS23430 at 452.976 kb on - strand, within EX31_RS23430 at 453.066 kb on + strand at 453.147 kb on + strand at 453.147 kb on + strand at 453.147 kb on + strand at 453.148 kb on - strand at 453.197 kb on + strand at 453.230 kb on + strand at 453.234 kb on + strand at 453.234 kb on + strand at 453.235 kb on - strand at 453.237 kb on + strand at 453.237 kb on + strand at 453.237 kb on + strand at 453.286 kb on + strand at 453.313 kb on + strand at 453.313 kb on + strand at 453.313 kb on + strand at 453.313 kb on + strand at 453.325 kb on + strand at 453.340 kb on - strand, within EX31_RS23435 at 453.483 kb on + strand, within EX31_RS23435 at 453.483 kb on + strand, within EX31_RS23435 at 453.483 kb on + strand, within EX31_RS23435 at 453.570 kb on - strand, within EX31_RS23435 at 453.572 kb on + strand, within EX31_RS23435 at 453.572 kb on + strand, within EX31_RS23435 at 453.572 kb on + strand, within EX31_RS23435 at 453.589 kb on + strand, within EX31_RS23435 at 453.609 kb on + strand, within EX31_RS23435 at 453.616 kb on + strand, within EX31_RS23435 at 453.616 kb on + strand, within EX31_RS23435 at 453.616 kb on + strand, within EX31_RS23435 at 453.616 kb on + strand, within EX31_RS23435 at 453.697 kb on + strand, within EX31_RS23435 at 453.697 kb on + strand, within EX31_RS23435 at 453.705 kb on + strand, within EX31_RS23435 at 453.705 kb on + strand, within EX31_RS23435 at 453.705 kb on + strand, within EX31_RS23435 at 453.706 kb on - strand, within EX31_RS23435 at 453.902 kb on + strand, within EX31_RS23435 at 453.903 kb on - strand, within EX31_RS23435 at 453.945 kb on + strand at 453.945 kb on + strand at 453.946 kb on - strand at 453.946 kb on - strand at 453.959 kb on + strand at 453.959 kb on + strand at 453.980 kb on + strand at 453.980 kb on + strand at 454.020 kb on + strand at 454.020 kb on + strand at 454.021 kb on - strand at 454.021 kb on - strand at 454.048 kb on - strand at 454.062 kb on + strand at 454.062 kb on + strand at 454.143 kb on + strand at 454.207 kb on + strand at 454.207 kb on + strand at 454.207 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 450,484 + -1.5 450,485 - -1.3 450,535 + -1.8 450,562 + -1.7 450,609 + +0.3 450,609 + +0.7 450,609 + -0.5 450,702 + +0.4 450,702 + -0.0 450,702 + +0.4 450,702 + -0.7 450,702 + +0.0 450,702 + -0.2 450,703 - +0.3 450,824 + EX31_RS23420 0.22 +0.1 450,825 - EX31_RS23420 0.22 -1.0 450,846 + EX31_RS23420 0.27 -1.1 450,846 + EX31_RS23420 0.27 +0.4 450,846 + EX31_RS23420 0.27 -1.1 450,846 + EX31_RS23420 0.27 -2.1 450,846 + EX31_RS23420 0.27 -0.8 450,846 + EX31_RS23420 0.27 -1.6 450,936 + EX31_RS23420 0.51 -1.8 450,936 + EX31_RS23420 0.51 -0.4 450,936 + EX31_RS23420 0.51 -2.1 450,985 + EX31_RS23420 0.63 +1.3 451,004 + EX31_RS23420 0.68 +1.4 451,126 + +0.3 451,151 + -0.5 451,206 + EX31_RS23425 0.24 -2.1 451,206 + EX31_RS23425 0.24 -0.7 451,206 + EX31_RS23425 0.24 -0.8 451,206 + EX31_RS23425 0.24 +0.5 451,206 + EX31_RS23425 0.24 -1.8 451,206 + EX31_RS23425 0.24 -1.2 451,206 + EX31_RS23425 0.24 -0.5 451,206 + EX31_RS23425 0.24 +0.4 451,206 + EX31_RS23425 0.24 -1.6 451,208 + EX31_RS23425 0.25 -0.2 451,208 + EX31_RS23425 0.25 -0.1 451,209 - EX31_RS23425 0.25 +1.0 451,217 + EX31_RS23425 0.27 +1.0 451,217 + EX31_RS23425 0.27 -0.1 451,217 + EX31_RS23425 0.27 +0.6 451,297 + EX31_RS23425 0.50 -0.6 451,297 + EX31_RS23425 0.50 -0.2 451,368 + EX31_RS23425 0.71 -0.4 451,431 + EX31_RS23425 0.89 -0.5 451,431 + EX31_RS23425 0.89 -2.8 451,435 + -0.3 451,435 + -1.3 451,440 + -0.1 451,467 + -1.8 451,467 + -1.7 451,467 + -0.5 451,467 + +0.2 451,607 + -0.8 451,680 + EX31_RS23430 0.12 -1.6 451,680 + EX31_RS23430 0.12 +0.8 451,680 + EX31_RS23430 0.12 +0.5 451,680 + EX31_RS23430 0.12 +0.1 451,680 + EX31_RS23430 0.12 -2.0 451,704 + EX31_RS23430 0.13 +0.9 451,704 + EX31_RS23430 0.13 -2.8 451,704 + EX31_RS23430 0.13 +1.1 451,704 + EX31_RS23430 0.13 -0.2 451,704 + EX31_RS23430 0.13 -2.4 451,705 - EX31_RS23430 0.13 +0.4 451,706 + EX31_RS23430 0.13 -1.5 451,731 + EX31_RS23430 0.15 -0.6 451,731 + EX31_RS23430 0.15 -0.1 451,731 + EX31_RS23430 0.15 +0.5 451,731 + EX31_RS23430 0.15 +0.1 451,731 + EX31_RS23430 0.15 -0.2 451,731 + EX31_RS23430 0.15 -0.3 451,731 + EX31_RS23430 0.15 +1.2 451,732 - EX31_RS23430 0.15 +1.0 451,732 - EX31_RS23430 0.15 +0.9 451,732 - EX31_RS23430 0.15 -0.1 451,733 + EX31_RS23430 0.15 +0.5 451,733 + EX31_RS23430 0.15 +0.6 451,733 + EX31_RS23430 0.15 -2.9 451,733 + EX31_RS23430 0.15 +0.3 451,733 + EX31_RS23430 0.15 -0.1 451,733 + EX31_RS23430 0.15 -2.0 451,733 + EX31_RS23430 0.15 -0.9 451,733 + EX31_RS23430 0.15 -1.5 451,815 + EX31_RS23430 0.19 -0.9 451,815 + EX31_RS23430 0.19 -0.7 451,816 - EX31_RS23430 0.19 -0.2 451,871 + EX31_RS23430 0.23 -0.3 451,920 + EX31_RS23430 0.25 -0.4 452,046 + EX31_RS23430 0.32 -0.8 452,123 + EX31_RS23430 0.37 -0.7 452,123 + EX31_RS23430 0.37 -0.5 452,351 + EX31_RS23430 0.50 -0.6 452,429 + EX31_RS23430 0.54 +0.4 452,535 + EX31_RS23430 0.60 +1.0 452,535 + EX31_RS23430 0.60 +1.9 452,535 + EX31_RS23430 0.60 +1.5 452,535 + EX31_RS23430 0.60 +1.1 452,535 + EX31_RS23430 0.60 -0.6 452,535 + EX31_RS23430 0.60 -0.4 452,535 + EX31_RS23430 0.60 -0.4 452,535 + EX31_RS23430 0.60 -1.8 452,535 + EX31_RS23430 0.60 -0.3 452,536 - EX31_RS23430 0.60 -0.5 452,738 + EX31_RS23430 0.72 -0.3 452,788 + EX31_RS23430 0.74 +1.3 452,788 + EX31_RS23430 0.74 -1.6 452,975 + EX31_RS23430 0.85 +0.9 452,975 + EX31_RS23430 0.85 +0.3 452,976 - EX31_RS23430 0.85 -1.1 453,066 + -0.1 453,147 + -0.6 453,147 + -1.6 453,147 + -0.5 453,148 - +2.3 453,197 + -0.1 453,230 + -1.8 453,234 + -0.8 453,234 + +0.6 453,235 - +0.5 453,237 + -0.8 453,237 + -0.3 453,237 + -2.9 453,286 + -1.5 453,313 + +0.3 453,313 + +0.3 453,313 + -0.2 453,313 + +1.3 453,325 + -0.8 453,340 - EX31_RS23435 0.11 +1.9 453,483 + EX31_RS23435 0.31 -0.5 453,483 + EX31_RS23435 0.31 +0.6 453,483 + EX31_RS23435 0.31 +1.9 453,570 - EX31_RS23435 0.43 +0.1 453,572 + EX31_RS23435 0.44 -1.7 453,572 + EX31_RS23435 0.44 -0.1 453,572 + EX31_RS23435 0.44 -1.2 453,589 + EX31_RS23435 0.46 +0.3 453,609 + EX31_RS23435 0.49 -0.9 453,616 + EX31_RS23435 0.50 +2.3 453,616 + EX31_RS23435 0.50 -1.2 453,616 + EX31_RS23435 0.50 -0.7 453,616 + EX31_RS23435 0.50 +0.6 453,697 + EX31_RS23435 0.61 +0.3 453,697 + EX31_RS23435 0.61 -0.5 453,705 + EX31_RS23435 0.62 +0.5 453,705 + EX31_RS23435 0.62 -0.1 453,705 + EX31_RS23435 0.62 -1.1 453,706 - EX31_RS23435 0.62 -0.8 453,902 + EX31_RS23435 0.90 +0.4 453,903 - EX31_RS23435 0.90 +0.9 453,945 + -2.4 453,945 + -0.6 453,946 - +2.4 453,946 - +2.2 453,959 + +0.1 453,959 + -0.6 453,980 + +0.7 453,980 + -0.6 454,020 + -0.2 454,020 + -0.0 454,021 - -2.2 454,021 - -0.1 454,048 - +1.7 454,062 + +1.0 454,062 + +0.0 454,143 + -0.2 454,207 + -0.1 454,207 + -1.6 454,207 + -1.3
Or see this region's nucleotide sequence