Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22695 and EX31_RS22700 are separated by 232 nucleotides EX31_RS22700 and EX31_RS22705 are separated by 81 nucleotides
EX31_RS22695: EX31_RS22695 - phosphoribosylformylglycinamidine cyclo-ligase, at 298,883 to 299,920
_RS22695
EX31_RS22700: EX31_RS22700 - uracil phosphoribosyltransferase, at 300,153 to 300,779
_RS22700
EX31_RS22705: EX31_RS22705 - uracil permease, at 300,861 to 302,153
_RS22705
Position (kb) 300
301 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 299.340 kb on + strand, within EX31_RS22695 at 299.475 kb on + strand, within EX31_RS22695 at 299.475 kb on + strand, within EX31_RS22695 at 299.476 kb on - strand, within EX31_RS22695 at 299.476 kb on - strand, within EX31_RS22695 at 299.476 kb on - strand, within EX31_RS22695 at 299.476 kb on - strand, within EX31_RS22695 at 299.530 kb on + strand, within EX31_RS22695 at 299.530 kb on + strand, within EX31_RS22695 at 299.595 kb on + strand, within EX31_RS22695 at 299.596 kb on - strand, within EX31_RS22695 at 299.598 kb on + strand, within EX31_RS22695 at 299.598 kb on + strand, within EX31_RS22695 at 299.598 kb on + strand, within EX31_RS22695 at 299.674 kb on + strand, within EX31_RS22695 at 299.675 kb on - strand, within EX31_RS22695 at 299.677 kb on + strand, within EX31_RS22695 at 299.677 kb on + strand, within EX31_RS22695 at 299.677 kb on + strand, within EX31_RS22695 at 299.678 kb on - strand, within EX31_RS22695 at 299.831 kb on + strand at 299.832 kb on - strand at 299.834 kb on + strand at 299.834 kb on + strand at 299.834 kb on + strand at 299.835 kb on - strand at 299.835 kb on - strand at 299.835 kb on - strand at 299.852 kb on + strand at 299.852 kb on + strand at 299.853 kb on - strand at 299.853 kb on - strand at 299.880 kb on - strand at 299.880 kb on - strand at 299.880 kb on - strand at 299.880 kb on - strand at 299.880 kb on - strand at 299.880 kb on - strand at 299.895 kb on + strand at 299.895 kb on + strand at 299.895 kb on + strand at 299.895 kb on + strand at 299.896 kb on - strand at 299.896 kb on - strand at 299.896 kb on - strand at 299.896 kb on - strand at 299.977 kb on + strand at 299.978 kb on - strand at 299.978 kb on - strand at 300.008 kb on + strand at 300.025 kb on + strand at 300.026 kb on - strand at 300.034 kb on + strand at 300.034 kb on + strand at 300.034 kb on + strand at 300.035 kb on - strand at 300.035 kb on - strand at 300.035 kb on - strand at 300.035 kb on - strand at 300.037 kb on - strand at 300.051 kb on - strand at 300.072 kb on - strand at 300.072 kb on - strand at 300.104 kb on + strand at 300.104 kb on + strand at 300.139 kb on + strand at 300.139 kb on + strand at 300.139 kb on + strand at 300.139 kb on + strand at 300.139 kb on + strand at 300.139 kb on + strand at 300.139 kb on + strand at 300.140 kb on - strand at 300.148 kb on - strand at 300.151 kb on + strand at 300.151 kb on + strand at 300.273 kb on + strand, within EX31_RS22700 at 300.273 kb on + strand, within EX31_RS22700 at 300.273 kb on + strand, within EX31_RS22700 at 300.273 kb on + strand, within EX31_RS22700 at 300.274 kb on - strand, within EX31_RS22700 at 300.274 kb on - strand, within EX31_RS22700 at 300.292 kb on + strand, within EX31_RS22700 at 300.292 kb on + strand, within EX31_RS22700 at 300.293 kb on - strand, within EX31_RS22700 at 300.351 kb on - strand, within EX31_RS22700 at 300.370 kb on + strand, within EX31_RS22700 at 300.370 kb on + strand, within EX31_RS22700 at 300.370 kb on + strand, within EX31_RS22700 at 300.371 kb on - strand, within EX31_RS22700 at 300.378 kb on - strand, within EX31_RS22700 at 300.413 kb on - strand, within EX31_RS22700 at 300.437 kb on + strand, within EX31_RS22700 at 300.490 kb on - strand, within EX31_RS22700 at 300.490 kb on - strand, within EX31_RS22700 at 300.510 kb on - strand, within EX31_RS22700 at 300.566 kb on + strand, within EX31_RS22700 at 300.566 kb on + strand, within EX31_RS22700 at 300.566 kb on + strand, within EX31_RS22700 at 300.566 kb on + strand, within EX31_RS22700 at 300.567 kb on - strand, within EX31_RS22700 at 300.614 kb on + strand, within EX31_RS22700 at 300.614 kb on + strand, within EX31_RS22700 at 300.614 kb on + strand, within EX31_RS22700 at 300.687 kb on + strand, within EX31_RS22700 at 300.687 kb on + strand, within EX31_RS22700 at 300.688 kb on - strand, within EX31_RS22700 at 300.719 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.726 kb on + strand at 300.727 kb on - strand at 300.763 kb on + strand at 300.763 kb on + strand at 300.763 kb on + strand at 300.763 kb on + strand at 300.763 kb on + strand at 300.763 kb on + strand at 300.771 kb on - strand at 300.777 kb on + strand at 300.777 kb on + strand at 300.777 kb on + strand at 300.978 kb on - strand at 300.978 kb on - strand at 301.015 kb on + strand, within EX31_RS22705 at 301.029 kb on + strand, within EX31_RS22705 at 301.029 kb on + strand, within EX31_RS22705 at 301.029 kb on + strand, within EX31_RS22705 at 301.033 kb on + strand, within EX31_RS22705 at 301.033 kb on + strand, within EX31_RS22705 at 301.033 kb on + strand, within EX31_RS22705 at 301.034 kb on - strand, within EX31_RS22705 at 301.034 kb on - strand, within EX31_RS22705 at 301.034 kb on - strand, within EX31_RS22705 at 301.043 kb on + strand, within EX31_RS22705 at 301.044 kb on - strand, within EX31_RS22705 at 301.044 kb on - strand, within EX31_RS22705 at 301.062 kb on + strand, within EX31_RS22705 at 301.063 kb on - strand, within EX31_RS22705 at 301.120 kb on - strand, within EX31_RS22705 at 301.120 kb on - strand, within EX31_RS22705 at 301.143 kb on - strand, within EX31_RS22705 at 301.184 kb on + strand, within EX31_RS22705 at 301.208 kb on + strand, within EX31_RS22705 at 301.216 kb on + strand, within EX31_RS22705 at 301.216 kb on + strand, within EX31_RS22705 at 301.337 kb on + strand, within EX31_RS22705 at 301.406 kb on + strand, within EX31_RS22705 at 301.437 kb on + strand, within EX31_RS22705 at 301.437 kb on + strand, within EX31_RS22705 at 301.438 kb on - strand, within EX31_RS22705 at 301.455 kb on - strand, within EX31_RS22705 at 301.455 kb on - strand, within EX31_RS22705 at 301.515 kb on + strand, within EX31_RS22705 at 301.515 kb on + strand, within EX31_RS22705 at 301.515 kb on + strand, within EX31_RS22705 at 301.515 kb on + strand, within EX31_RS22705 at 301.515 kb on + strand, within EX31_RS22705 at 301.515 kb on + strand, within EX31_RS22705 at 301.516 kb on - strand, within EX31_RS22705 at 301.516 kb on - strand, within EX31_RS22705 at 301.516 kb on - strand, within EX31_RS22705 at 301.517 kb on + strand, within EX31_RS22705 at 301.517 kb on + strand, within EX31_RS22705 at 301.517 kb on + strand, within EX31_RS22705 at 301.518 kb on - strand, within EX31_RS22705 at 301.630 kb on + strand, within EX31_RS22705 at 301.684 kb on + strand, within EX31_RS22705 at 301.684 kb on + strand, within EX31_RS22705 at 301.715 kb on + strand, within EX31_RS22705 at 301.716 kb on - strand, within EX31_RS22705 at 301.722 kb on + strand, within EX31_RS22705 at 301.722 kb on + strand, within EX31_RS22705 at 301.722 kb on + strand, within EX31_RS22705 at 301.759 kb on + strand, within EX31_RS22705 at 301.759 kb on + strand, within EX31_RS22705 at 301.759 kb on + strand, within EX31_RS22705 at 301.759 kb on + strand, within EX31_RS22705 at 301.760 kb on - strand, within EX31_RS22705 at 301.760 kb on - strand, within EX31_RS22705 at 301.760 kb on - strand, within EX31_RS22705 at 301.760 kb on - strand, within EX31_RS22705 at 301.760 kb on - strand, within EX31_RS22705 at 301.761 kb on + strand, within EX31_RS22705 at 301.761 kb on + strand, within EX31_RS22705 at 301.761 kb on + strand, within EX31_RS22705 at 301.761 kb on + strand, within EX31_RS22705 at 301.761 kb on + strand, within EX31_RS22705 at 301.761 kb on + strand, within EX31_RS22705 at 301.762 kb on - strand, within EX31_RS22705 at 301.775 kb on + strand, within EX31_RS22705
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 299,340 + EX31_RS22695 0.44 -2.9 299,475 + EX31_RS22695 0.57 -3.0 299,475 + EX31_RS22695 0.57 -1.1 299,476 - EX31_RS22695 0.57 -3.4 299,476 - EX31_RS22695 0.57 -2.3 299,476 - EX31_RS22695 0.57 -2.6 299,476 - EX31_RS22695 0.57 -2.6 299,530 + EX31_RS22695 0.62 -3.2 299,530 + EX31_RS22695 0.62 -2.8 299,595 + EX31_RS22695 0.69 -1.4 299,596 - EX31_RS22695 0.69 -3.2 299,598 + EX31_RS22695 0.69 -2.7 299,598 + EX31_RS22695 0.69 -0.1 299,598 + EX31_RS22695 0.69 -2.4 299,674 + EX31_RS22695 0.76 -2.6 299,675 - EX31_RS22695 0.76 -3.4 299,677 + EX31_RS22695 0.76 -1.8 299,677 + EX31_RS22695 0.76 -1.6 299,677 + EX31_RS22695 0.76 -1.1 299,678 - EX31_RS22695 0.77 -2.8 299,831 + -2.8 299,832 - -1.8 299,834 + -3.5 299,834 + -3.7 299,834 + -2.3 299,835 - -3.5 299,835 - -3.6 299,835 - -4.2 299,852 + -0.4 299,852 + -2.7 299,853 - -2.8 299,853 - +1.9 299,880 - -3.2 299,880 - -1.6 299,880 - -3.2 299,880 - -3.0 299,880 - -3.6 299,880 - -3.5 299,895 + -2.0 299,895 + -4.1 299,895 + -3.6 299,895 + -2.0 299,896 - -3.0 299,896 - -3.0 299,896 - -3.3 299,896 - -4.0 299,977 + -2.9 299,978 - -1.2 299,978 - -2.4 300,008 + +2.1 300,025 + -0.6 300,026 - -1.1 300,034 + +0.6 300,034 + -0.7 300,034 + -0.8 300,035 - +0.2 300,035 - +1.6 300,035 - -0.1 300,035 - -0.4 300,037 - -0.2 300,051 - +0.8 300,072 - +0.3 300,072 - +0.3 300,104 + -0.6 300,104 + -0.7 300,139 + -0.6 300,139 + -0.4 300,139 + -0.2 300,139 + -0.2 300,139 + -0.7 300,139 + -0.4 300,139 + +0.6 300,140 - -0.7 300,148 - -0.4 300,151 + -0.7 300,151 + -0.8 300,273 + EX31_RS22700 0.19 -1.5 300,273 + EX31_RS22700 0.19 -0.3 300,273 + EX31_RS22700 0.19 +0.2 300,273 + EX31_RS22700 0.19 -0.4 300,274 - EX31_RS22700 0.19 +1.9 300,274 - EX31_RS22700 0.19 +0.4 300,292 + EX31_RS22700 0.22 -0.4 300,292 + EX31_RS22700 0.22 -0.8 300,293 - EX31_RS22700 0.22 +0.8 300,351 - EX31_RS22700 0.32 -0.3 300,370 + EX31_RS22700 0.35 -2.2 300,370 + EX31_RS22700 0.35 -1.3 300,370 + EX31_RS22700 0.35 +0.9 300,371 - EX31_RS22700 0.35 +0.9 300,378 - EX31_RS22700 0.36 +1.9 300,413 - EX31_RS22700 0.41 +0.3 300,437 + EX31_RS22700 0.45 -0.7 300,490 - EX31_RS22700 0.54 +0.3 300,490 - EX31_RS22700 0.54 -1.1 300,510 - EX31_RS22700 0.57 +0.3 300,566 + EX31_RS22700 0.66 -0.8 300,566 + EX31_RS22700 0.66 +0.6 300,566 + EX31_RS22700 0.66 -0.0 300,566 + EX31_RS22700 0.66 -0.8 300,567 - EX31_RS22700 0.66 -0.4 300,614 + EX31_RS22700 0.74 -0.9 300,614 + EX31_RS22700 0.74 -1.2 300,614 + EX31_RS22700 0.74 -0.1 300,687 + EX31_RS22700 0.85 -2.9 300,687 + EX31_RS22700 0.85 -1.2 300,688 - EX31_RS22700 0.85 +1.0 300,719 + -0.2 300,726 + -0.3 300,726 + +0.0 300,726 + -0.1 300,726 + -1.1 300,726 + +0.8 300,726 + +0.5 300,726 + +0.1 300,726 + -0.2 300,726 + +0.2 300,727 - -1.9 300,763 + -0.1 300,763 + -0.0 300,763 + +0.5 300,763 + +0.5 300,763 + -0.8 300,763 + +1.9 300,771 - -0.9 300,777 + -1.1 300,777 + -2.0 300,777 + +0.7 300,978 - +0.1 300,978 - -2.0 301,015 + EX31_RS22705 0.12 -0.5 301,029 + EX31_RS22705 0.13 +0.1 301,029 + EX31_RS22705 0.13 +0.4 301,029 + EX31_RS22705 0.13 +0.4 301,033 + EX31_RS22705 0.13 +0.0 301,033 + EX31_RS22705 0.13 +0.4 301,033 + EX31_RS22705 0.13 +0.3 301,034 - EX31_RS22705 0.13 -0.1 301,034 - EX31_RS22705 0.13 -0.5 301,034 - EX31_RS22705 0.13 +1.1 301,043 + EX31_RS22705 0.14 -0.9 301,044 - EX31_RS22705 0.14 -0.0 301,044 - EX31_RS22705 0.14 -0.1 301,062 + EX31_RS22705 0.16 -1.1 301,063 - EX31_RS22705 0.16 +0.7 301,120 - EX31_RS22705 0.20 +0.1 301,120 - EX31_RS22705 0.20 -1.4 301,143 - EX31_RS22705 0.22 -0.7 301,184 + EX31_RS22705 0.25 +0.2 301,208 + EX31_RS22705 0.27 +1.4 301,216 + EX31_RS22705 0.27 -0.9 301,216 + EX31_RS22705 0.27 -0.9 301,337 + EX31_RS22705 0.37 +0.1 301,406 + EX31_RS22705 0.42 +0.2 301,437 + EX31_RS22705 0.45 +0.4 301,437 + EX31_RS22705 0.45 +0.9 301,438 - EX31_RS22705 0.45 -0.4 301,455 - EX31_RS22705 0.46 +0.5 301,455 - EX31_RS22705 0.46 +0.1 301,515 + EX31_RS22705 0.51 +0.3 301,515 + EX31_RS22705 0.51 +0.4 301,515 + EX31_RS22705 0.51 -0.7 301,515 + EX31_RS22705 0.51 -0.9 301,515 + EX31_RS22705 0.51 +0.1 301,515 + EX31_RS22705 0.51 -0.6 301,516 - EX31_RS22705 0.51 -1.1 301,516 - EX31_RS22705 0.51 -0.7 301,516 - EX31_RS22705 0.51 +0.3 301,517 + EX31_RS22705 0.51 -1.3 301,517 + EX31_RS22705 0.51 -0.6 301,517 + EX31_RS22705 0.51 +0.7 301,518 - EX31_RS22705 0.51 +0.1 301,630 + EX31_RS22705 0.59 +0.2 301,684 + EX31_RS22705 0.64 +0.3 301,684 + EX31_RS22705 0.64 -1.1 301,715 + EX31_RS22705 0.66 -1.4 301,716 - EX31_RS22705 0.66 -0.6 301,722 + EX31_RS22705 0.67 -0.2 301,722 + EX31_RS22705 0.67 +0.3 301,722 + EX31_RS22705 0.67 +0.6 301,759 + EX31_RS22705 0.69 +1.0 301,759 + EX31_RS22705 0.69 -1.8 301,759 + EX31_RS22705 0.69 +0.0 301,759 + EX31_RS22705 0.69 -2.0 301,760 - EX31_RS22705 0.70 -0.8 301,760 - EX31_RS22705 0.70 +1.2 301,760 - EX31_RS22705 0.70 +0.0 301,760 - EX31_RS22705 0.70 +1.4 301,760 - EX31_RS22705 0.70 -0.1 301,761 + EX31_RS22705 0.70 -0.4 301,761 + EX31_RS22705 0.70 -1.2 301,761 + EX31_RS22705 0.70 +0.1 301,761 + EX31_RS22705 0.70 -0.2 301,761 + EX31_RS22705 0.70 +0.9 301,761 + EX31_RS22705 0.70 +0.1 301,762 - EX31_RS22705 0.70 -2.1 301,775 + EX31_RS22705 0.71 -1.4
Or see this region's nucleotide sequence