Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15800 and EX31_RS15805 are separated by 54 nucleotides EX31_RS15805 and EX31_RS15810 are separated by 19 nucleotides EX31_RS15810 and EX31_RS15815 are separated by 5 nucleotides EX31_RS15815 and EX31_RS15820 overlap by 4 nucleotides
EX31_RS15800: EX31_RS15800 - guanylate kinase, at 12,384 to 13,007
_RS15800
EX31_RS15805: EX31_RS15805 - DNA-directed RNA polymerase subunit omega, at 13,062 to 13,337
_RS15805
EX31_RS15810: EX31_RS15810 - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 13,357 to 15,465
_RS15810
EX31_RS15815: EX31_RS15815 - tRNA (guanosine(18)-2'-O)-methyltransferase TrmH, at 15,471 to 16,169
_RS15815
EX31_RS15820: EX31_RS15820 - ATP-dependent DNA helicase RecG, at 16,166 to 18,247
_RS15820
Position (kb) 13
14
15
16 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 13.020 kb on + strand at 13.020 kb on + strand at 13.023 kb on + strand at 13.023 kb on + strand at 13.023 kb on + strand at 13.023 kb on + strand at 13.052 kb on + strand at 13.052 kb on + strand at 13.052 kb on + strand at 13.052 kb on + strand at 13.052 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.059 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.061 kb on + strand at 13.072 kb on + strand at 13.072 kb on + strand at 13.072 kb on + strand at 13.163 kb on + strand, within EX31_RS15805 at 13.204 kb on + strand, within EX31_RS15805 at 13.204 kb on + strand, within EX31_RS15805 at 13.204 kb on + strand, within EX31_RS15805 at 13.204 kb on + strand, within EX31_RS15805 at 13.204 kb on + strand, within EX31_RS15805 at 13.204 kb on + strand, within EX31_RS15805 at 13.204 kb on + strand, within EX31_RS15805 at 13.335 kb on + strand at 13.335 kb on + strand at 13.360 kb on + strand at 14.862 kb on + strand, within EX31_RS15810 at 14.898 kb on + strand, within EX31_RS15810 at 14.899 kb on - strand, within EX31_RS15810 at 15.018 kb on + strand, within EX31_RS15810 at 15.019 kb on - strand, within EX31_RS15810 at 15.031 kb on - strand, within EX31_RS15810 at 15.050 kb on + strand, within EX31_RS15810 at 15.050 kb on + strand, within EX31_RS15810 at 15.050 kb on + strand, within EX31_RS15810 at 15.050 kb on + strand, within EX31_RS15810 at 15.051 kb on - strand, within EX31_RS15810 at 15.153 kb on + strand, within EX31_RS15810 at 15.252 kb on + strand, within EX31_RS15810 at 15.252 kb on + strand, within EX31_RS15810 at 15.252 kb on + strand, within EX31_RS15810 at 15.252 kb on + strand, within EX31_RS15810 at 15.253 kb on - strand, within EX31_RS15810 at 15.253 kb on - strand, within EX31_RS15810 at 15.253 kb on - strand, within EX31_RS15810 at 15.253 kb on - strand, within EX31_RS15810 at 15.253 kb on - strand, within EX31_RS15810 at 15.253 kb on - strand, within EX31_RS15810 at 15.297 kb on + strand at 15.297 kb on + strand at 15.297 kb on + strand at 15.298 kb on - strand at 15.298 kb on - strand at 15.298 kb on - strand at 15.356 kb on - strand at 15.406 kb on - strand at 15.415 kb on - strand at 15.470 kb on + strand at 15.471 kb on - strand at 15.471 kb on - strand at 15.486 kb on + strand at 15.554 kb on + strand, within EX31_RS15815 at 15.554 kb on + strand, within EX31_RS15815 at 15.554 kb on + strand, within EX31_RS15815 at 15.555 kb on - strand, within EX31_RS15815 at 15.563 kb on + strand, within EX31_RS15815 at 15.563 kb on + strand, within EX31_RS15815 at 15.563 kb on + strand, within EX31_RS15815 at 15.563 kb on + strand, within EX31_RS15815 at 15.563 kb on + strand, within EX31_RS15815 at 15.563 kb on + strand, within EX31_RS15815 at 15.564 kb on - strand, within EX31_RS15815 at 15.630 kb on - strand, within EX31_RS15815 at 15.630 kb on - strand, within EX31_RS15815 at 15.798 kb on + strand, within EX31_RS15815 at 15.799 kb on - strand, within EX31_RS15815 at 15.799 kb on - strand, within EX31_RS15815 at 15.817 kb on + strand, within EX31_RS15815 at 15.817 kb on + strand, within EX31_RS15815 at 15.817 kb on + strand, within EX31_RS15815 at 15.817 kb on + strand, within EX31_RS15815 at 15.818 kb on - strand, within EX31_RS15815 at 15.818 kb on - strand, within EX31_RS15815 at 15.878 kb on + strand, within EX31_RS15815 at 15.878 kb on + strand, within EX31_RS15815 at 15.878 kb on + strand, within EX31_RS15815 at 15.879 kb on - strand, within EX31_RS15815 at 15.900 kb on - strand, within EX31_RS15815 at 15.900 kb on - strand, within EX31_RS15815 at 15.900 kb on - strand, within EX31_RS15815 at 15.900 kb on - strand, within EX31_RS15815 at 15.938 kb on + strand, within EX31_RS15815 at 15.977 kb on + strand, within EX31_RS15815 at 15.978 kb on - strand, within EX31_RS15815 at 15.978 kb on - strand, within EX31_RS15815 at 15.981 kb on + strand, within EX31_RS15815 at 15.982 kb on - strand, within EX31_RS15815 at 15.982 kb on - strand, within EX31_RS15815 at 15.982 kb on - strand, within EX31_RS15815 at 15.982 kb on - strand, within EX31_RS15815 at 16.290 kb on + strand at 16.290 kb on + strand at 16.290 kb on + strand at 16.290 kb on + strand at 16.290 kb on + strand at 16.290 kb on + strand at 16.291 kb on - strand at 16.295 kb on + strand at 16.296 kb on - strand at 16.333 kb on - strand at 16.333 kb on - strand at 16.356 kb on + strand at 16.356 kb on + strand at 16.356 kb on + strand at 16.356 kb on + strand at 16.356 kb on + strand at 16.356 kb on + strand at 16.356 kb on + strand at 16.356 kb on + strand at 16.356 kb on + strand at 16.356 kb on + strand at 16.356 kb on + strand at 16.357 kb on - strand at 16.357 kb on - strand at 16.357 kb on - strand at 16.357 kb on - strand at 16.357 kb on - strand at 16.357 kb on - strand at 16.375 kb on + strand, within EX31_RS15820 at 16.375 kb on + strand, within EX31_RS15820 at 16.375 kb on + strand, within EX31_RS15820 at 16.376 kb on - strand, within EX31_RS15820 at 16.376 kb on - strand, within EX31_RS15820 at 16.382 kb on - strand, within EX31_RS15820 at 16.382 kb on - strand, within EX31_RS15820 at 16.419 kb on + strand, within EX31_RS15820 at 16.419 kb on + strand, within EX31_RS15820 at 16.419 kb on + strand, within EX31_RS15820 at 16.419 kb on + strand, within EX31_RS15820 at 16.419 kb on + strand, within EX31_RS15820 at 16.420 kb on - strand, within EX31_RS15820 at 16.420 kb on - strand, within EX31_RS15820 at 16.420 kb on - strand, within EX31_RS15820 at 16.420 kb on - strand, within EX31_RS15820 at 16.420 kb on - strand, within EX31_RS15820 at 16.447 kb on - strand, within EX31_RS15820
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 13,020 + -0.3 13,020 + -0.6 13,023 + -0.9 13,023 + -1.8 13,023 + -1.3 13,023 + -0.2 13,052 + +0.8 13,052 + -1.6 13,052 + -0.9 13,052 + -0.9 13,052 + -0.0 13,059 + -1.0 13,059 + -0.5 13,059 + +0.2 13,059 + +0.8 13,059 + -1.4 13,059 + +0.4 13,059 + +0.2 13,059 + -1.5 13,059 + -2.6 13,059 + -0.9 13,059 + -0.1 13,059 + +0.2 13,059 + +0.0 13,059 + -1.0 13,059 + -1.0 13,059 + -2.5 13,059 + -1.1 13,059 + +0.0 13,059 + -1.6 13,059 + +0.5 13,059 + -1.6 13,059 + -1.6 13,059 + -0.8 13,059 + -0.9 13,059 + +0.0 13,059 + +0.9 13,059 + -0.9 13,059 + -1.7 13,059 + -1.1 13,059 + -0.9 13,059 + -1.9 13,059 + -1.8 13,059 + -0.2 13,059 + -0.9 13,059 + +0.1 13,059 + -0.6 13,059 + -2.2 13,059 + -0.1 13,059 + -1.5 13,059 + -2.3 13,059 + -0.6 13,059 + -1.3 13,059 + -1.0 13,059 + -2.5 13,059 + -2.0 13,059 + -1.2 13,059 + -0.5 13,059 + -0.9 13,059 + -2.1 13,059 + +0.4 13,059 + -0.7 13,059 + +0.0 13,059 + -0.4 13,059 + -1.2 13,059 + -0.3 13,059 + -0.8 13,059 + -1.9 13,061 + -0.8 13,061 + +1.7 13,061 + -0.5 13,061 + -0.7 13,061 + -1.6 13,061 + -1.0 13,061 + -1.1 13,061 + -1.6 13,061 + -0.2 13,061 + -0.9 13,061 + +0.0 13,061 + -0.3 13,061 + -0.2 13,061 + +0.0 13,061 + -0.4 13,061 + -0.2 13,061 + -2.9 13,061 + -0.5 13,061 + -0.6 13,072 + -2.1 13,072 + -0.9 13,072 + -0.6 13,163 + EX31_RS15805 0.37 -1.9 13,204 + EX31_RS15805 0.51 -0.8 13,204 + EX31_RS15805 0.51 +0.6 13,204 + EX31_RS15805 0.51 -0.2 13,204 + EX31_RS15805 0.51 -2.0 13,204 + EX31_RS15805 0.51 -1.9 13,204 + EX31_RS15805 0.51 -0.8 13,204 + EX31_RS15805 0.51 -1.0 13,335 + -0.8 13,335 + -0.2 13,360 + -1.0 14,862 + EX31_RS15810 0.71 +0.2 14,898 + EX31_RS15810 0.73 +1.8 14,899 - EX31_RS15810 0.73 +0.1 15,018 + EX31_RS15810 0.79 -0.8 15,019 - EX31_RS15810 0.79 -1.3 15,031 - EX31_RS15810 0.79 -1.4 15,050 + EX31_RS15810 0.80 -0.3 15,050 + EX31_RS15810 0.80 -1.1 15,050 + EX31_RS15810 0.80 +0.8 15,050 + EX31_RS15810 0.80 +0.5 15,051 - EX31_RS15810 0.80 -0.8 15,153 + EX31_RS15810 0.85 -0.6 15,252 + EX31_RS15810 0.90 -0.5 15,252 + EX31_RS15810 0.90 +0.8 15,252 + EX31_RS15810 0.90 -0.4 15,252 + EX31_RS15810 0.90 -0.4 15,253 - EX31_RS15810 0.90 +0.7 15,253 - EX31_RS15810 0.90 -1.8 15,253 - EX31_RS15810 0.90 -0.8 15,253 - EX31_RS15810 0.90 -0.7 15,253 - EX31_RS15810 0.90 -0.8 15,253 - EX31_RS15810 0.90 +1.0 15,297 + -1.2 15,297 + -0.2 15,297 + -0.6 15,298 - -1.0 15,298 - -1.5 15,298 - +0.5 15,356 - -0.3 15,406 - -0.6 15,415 - +0.0 15,470 + +0.6 15,471 - -0.6 15,471 - +0.8 15,486 + +0.9 15,554 + EX31_RS15815 0.12 -0.5 15,554 + EX31_RS15815 0.12 -0.3 15,554 + EX31_RS15815 0.12 +0.8 15,555 - EX31_RS15815 0.12 +0.4 15,563 + EX31_RS15815 0.13 -0.1 15,563 + EX31_RS15815 0.13 +1.8 15,563 + EX31_RS15815 0.13 +0.2 15,563 + EX31_RS15815 0.13 -1.0 15,563 + EX31_RS15815 0.13 +0.5 15,563 + EX31_RS15815 0.13 +0.5 15,564 - EX31_RS15815 0.13 -1.3 15,630 - EX31_RS15815 0.23 +0.2 15,630 - EX31_RS15815 0.23 +0.1 15,798 + EX31_RS15815 0.47 +0.5 15,799 - EX31_RS15815 0.47 -1.0 15,799 - EX31_RS15815 0.47 +1.5 15,817 + EX31_RS15815 0.49 -0.6 15,817 + EX31_RS15815 0.49 -0.0 15,817 + EX31_RS15815 0.49 -0.2 15,817 + EX31_RS15815 0.49 -0.5 15,818 - EX31_RS15815 0.50 +0.0 15,818 - EX31_RS15815 0.50 -0.4 15,878 + EX31_RS15815 0.58 -0.3 15,878 + EX31_RS15815 0.58 +0.5 15,878 + EX31_RS15815 0.58 +0.8 15,879 - EX31_RS15815 0.58 -1.5 15,900 - EX31_RS15815 0.61 -0.8 15,900 - EX31_RS15815 0.61 -0.2 15,900 - EX31_RS15815 0.61 -0.5 15,900 - EX31_RS15815 0.61 +0.2 15,938 + EX31_RS15815 0.67 -0.0 15,977 + EX31_RS15815 0.72 -0.3 15,978 - EX31_RS15815 0.73 -0.5 15,978 - EX31_RS15815 0.73 -1.0 15,981 + EX31_RS15815 0.73 +0.2 15,982 - EX31_RS15815 0.73 -0.6 15,982 - EX31_RS15815 0.73 -0.6 15,982 - EX31_RS15815 0.73 +0.5 15,982 - EX31_RS15815 0.73 +0.4 16,290 + +0.6 16,290 + -0.2 16,290 + -0.8 16,290 + +0.1 16,290 + +0.4 16,290 + +1.1 16,291 - -0.5 16,295 + -0.8 16,296 - +0.8 16,333 - +0.5 16,333 - +1.0 16,356 + +0.5 16,356 + +0.1 16,356 + +0.4 16,356 + -0.2 16,356 + +0.6 16,356 + -0.3 16,356 + -0.0 16,356 + -0.5 16,356 + -1.6 16,356 + -0.1 16,356 + -0.0 16,357 - -2.3 16,357 - -1.3 16,357 - -0.7 16,357 - -0.1 16,357 - -0.1 16,357 - +0.2 16,375 + EX31_RS15820 0.10 -0.4 16,375 + EX31_RS15820 0.10 -0.1 16,375 + EX31_RS15820 0.10 -1.2 16,376 - EX31_RS15820 0.10 +0.3 16,376 - EX31_RS15820 0.10 -0.4 16,382 - EX31_RS15820 0.10 -0.6 16,382 - EX31_RS15820 0.10 -0.8 16,419 + EX31_RS15820 0.12 +0.6 16,419 + EX31_RS15820 0.12 -0.1 16,419 + EX31_RS15820 0.12 -0.0 16,419 + EX31_RS15820 0.12 +0.3 16,419 + EX31_RS15820 0.12 +0.2 16,420 - EX31_RS15820 0.12 +0.6 16,420 - EX31_RS15820 0.12 -1.2 16,420 - EX31_RS15820 0.12 -0.5 16,420 - EX31_RS15820 0.12 -0.6 16,420 - EX31_RS15820 0.12 -1.1 16,447 - EX31_RS15820 0.13 +0.6
Or see this region's nucleotide sequence