Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11455 and EX31_RS11460 are separated by 5 nucleotides EX31_RS11460 and EX31_RS11465 overlap by 19 nucleotides EX31_RS11465 and EX31_RS11470 are separated by 23 nucleotides EX31_RS11470 and EX31_RS11475 are separated by 118 nucleotides
EX31_RS11455: EX31_RS11455 - imidazole glycerol phosphate synthase subunit HisH, at 225,546 to 226,136
_RS11455
EX31_RS11460: EX31_RS11460 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 226,142 to 226,879
_RS11460
EX31_RS11465: EX31_RS11465 - imidazole glycerol phosphate synthase subunit HisF, at 226,861 to 227,634
_RS11465
EX31_RS11470: EX31_RS11470 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 227,658 to 228,272
_RS11470
EX31_RS11475: EX31_RS11475 - NADP-dependent phosphogluconate dehydrogenase, at 228,391 to 229,800
_RS11475
Position (kb) 226
227
228 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 225.891 kb on - strand, within EX31_RS11455 at 225.891 kb on - strand, within EX31_RS11455 at 225.891 kb on - strand, within EX31_RS11455 at 225.932 kb on - strand, within EX31_RS11455 at 225.932 kb on - strand, within EX31_RS11455 at 225.957 kb on + strand, within EX31_RS11455 at 225.957 kb on + strand, within EX31_RS11455 at 225.957 kb on + strand, within EX31_RS11455 at 225.958 kb on - strand, within EX31_RS11455 at 225.958 kb on - strand, within EX31_RS11455 at 225.964 kb on - strand, within EX31_RS11455 at 225.964 kb on - strand, within EX31_RS11455 at 225.964 kb on - strand, within EX31_RS11455 at 226.020 kb on + strand, within EX31_RS11455 at 226.134 kb on + strand at 226.134 kb on + strand at 226.134 kb on + strand at 226.134 kb on + strand at 226.134 kb on + strand at 226.135 kb on - strand at 226.135 kb on - strand at 226.135 kb on - strand at 226.147 kb on + strand at 226.202 kb on + strand at 226.202 kb on + strand at 226.345 kb on - strand, within EX31_RS11460 at 226.530 kb on + strand, within EX31_RS11460 at 226.530 kb on + strand, within EX31_RS11460 at 226.531 kb on - strand, within EX31_RS11460 at 226.550 kb on - strand, within EX31_RS11460 at 226.619 kb on + strand, within EX31_RS11460 at 226.620 kb on - strand, within EX31_RS11460 at 226.657 kb on + strand, within EX31_RS11460 at 226.840 kb on + strand at 226.841 kb on - strand at 226.877 kb on + strand at 226.922 kb on + strand at 226.922 kb on + strand at 226.922 kb on + strand at 227.008 kb on + strand, within EX31_RS11465 at 227.008 kb on + strand, within EX31_RS11465 at 227.039 kb on + strand, within EX31_RS11465 at 227.047 kb on - strand, within EX31_RS11465 at 227.076 kb on + strand, within EX31_RS11465 at 227.076 kb on + strand, within EX31_RS11465 at 227.082 kb on + strand, within EX31_RS11465 at 227.109 kb on + strand, within EX31_RS11465 at 227.109 kb on + strand, within EX31_RS11465 at 227.109 kb on + strand, within EX31_RS11465 at 227.154 kb on + strand, within EX31_RS11465 at 227.154 kb on + strand, within EX31_RS11465 at 227.218 kb on + strand, within EX31_RS11465 at 227.218 kb on + strand, within EX31_RS11465 at 227.218 kb on + strand, within EX31_RS11465 at 227.218 kb on + strand, within EX31_RS11465 at 227.218 kb on + strand, within EX31_RS11465 at 227.218 kb on + strand, within EX31_RS11465 at 227.218 kb on + strand, within EX31_RS11465 at 227.225 kb on + strand, within EX31_RS11465 at 227.225 kb on + strand, within EX31_RS11465 at 227.225 kb on + strand, within EX31_RS11465 at 227.225 kb on + strand, within EX31_RS11465 at 227.233 kb on - strand, within EX31_RS11465 at 227.233 kb on - strand, within EX31_RS11465 at 227.279 kb on - strand, within EX31_RS11465 at 227.313 kb on + strand, within EX31_RS11465 at 227.313 kb on + strand, within EX31_RS11465 at 227.314 kb on - strand, within EX31_RS11465 at 227.316 kb on + strand, within EX31_RS11465 at 227.316 kb on + strand, within EX31_RS11465 at 227.316 kb on + strand, within EX31_RS11465 at 227.316 kb on + strand, within EX31_RS11465 at 227.391 kb on + strand, within EX31_RS11465 at 227.391 kb on + strand, within EX31_RS11465 at 227.574 kb on + strand at 227.574 kb on + strand at 227.599 kb on + strand at 227.600 kb on - strand at 227.647 kb on + strand at 227.647 kb on + strand at 227.647 kb on + strand at 227.648 kb on - strand at 227.763 kb on + strand, within EX31_RS11470 at 227.763 kb on + strand, within EX31_RS11470 at 227.765 kb on + strand, within EX31_RS11470 at 227.765 kb on + strand, within EX31_RS11470 at 227.765 kb on + strand, within EX31_RS11470 at 227.766 kb on - strand, within EX31_RS11470 at 227.810 kb on - strand, within EX31_RS11470 at 228.057 kb on + strand, within EX31_RS11470 at 228.059 kb on + strand, within EX31_RS11470 at 228.270 kb on + strand at 228.371 kb on - strand at 228.371 kb on - strand at 228.371 kb on - strand at 228.371 kb on - strand at 228.392 kb on + strand at 228.393 kb on - strand at 228.393 kb on - strand at 228.416 kb on - strand at 228.416 kb on - strand at 228.441 kb on - strand at 228.441 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 225,891 - EX31_RS11455 0.58 -1.1 225,891 - EX31_RS11455 0.58 -1.0 225,891 - EX31_RS11455 0.58 -0.8 225,932 - EX31_RS11455 0.65 -1.9 225,932 - EX31_RS11455 0.65 +0.6 225,957 + EX31_RS11455 0.70 -1.5 225,957 + EX31_RS11455 0.70 -2.6 225,957 + EX31_RS11455 0.70 -1.0 225,958 - EX31_RS11455 0.70 -3.4 225,958 - EX31_RS11455 0.70 -1.0 225,964 - EX31_RS11455 0.71 -2.1 225,964 - EX31_RS11455 0.71 -3.2 225,964 - EX31_RS11455 0.71 -2.4 226,020 + EX31_RS11455 0.80 -2.4 226,134 + -1.5 226,134 + -2.9 226,134 + -2.4 226,134 + -0.8 226,134 + -3.5 226,135 - -1.3 226,135 - -2.1 226,135 - -0.8 226,147 + -2.3 226,202 + -1.4 226,202 + -1.4 226,345 - EX31_RS11460 0.28 -2.1 226,530 + EX31_RS11460 0.53 -2.2 226,530 + EX31_RS11460 0.53 -0.2 226,531 - EX31_RS11460 0.53 -2.4 226,550 - EX31_RS11460 0.55 -2.7 226,619 + EX31_RS11460 0.65 -1.0 226,620 - EX31_RS11460 0.65 -1.0 226,657 + EX31_RS11460 0.70 -3.1 226,840 + -3.1 226,841 - -1.0 226,877 + -1.1 226,922 + -0.8 226,922 + -0.5 226,922 + +0.8 227,008 + EX31_RS11465 0.19 -2.4 227,008 + EX31_RS11465 0.19 -0.8 227,039 + EX31_RS11465 0.23 -0.8 227,047 - EX31_RS11465 0.24 -0.2 227,076 + EX31_RS11465 0.28 -0.2 227,076 + EX31_RS11465 0.28 -1.6 227,082 + EX31_RS11465 0.29 -3.6 227,109 + EX31_RS11465 0.32 -0.8 227,109 + EX31_RS11465 0.32 -2.5 227,109 + EX31_RS11465 0.32 -2.4 227,154 + EX31_RS11465 0.38 -2.9 227,154 + EX31_RS11465 0.38 -1.6 227,218 + EX31_RS11465 0.46 -3.0 227,218 + EX31_RS11465 0.46 -3.6 227,218 + EX31_RS11465 0.46 -3.1 227,218 + EX31_RS11465 0.46 +0.2 227,218 + EX31_RS11465 0.46 -0.2 227,218 + EX31_RS11465 0.46 -0.8 227,218 + EX31_RS11465 0.46 -1.6 227,225 + EX31_RS11465 0.47 -2.4 227,225 + EX31_RS11465 0.47 -2.2 227,225 + EX31_RS11465 0.47 -1.4 227,225 + EX31_RS11465 0.47 -3.1 227,233 - EX31_RS11465 0.48 -1.6 227,233 - EX31_RS11465 0.48 -1.4 227,279 - EX31_RS11465 0.54 -1.6 227,313 + EX31_RS11465 0.58 -3.1 227,313 + EX31_RS11465 0.58 -2.0 227,314 - EX31_RS11465 0.59 -0.2 227,316 + EX31_RS11465 0.59 -2.7 227,316 + EX31_RS11465 0.59 -2.0 227,316 + EX31_RS11465 0.59 -1.9 227,316 + EX31_RS11465 0.59 -2.1 227,391 + EX31_RS11465 0.68 -2.4 227,391 + EX31_RS11465 0.68 -3.4 227,574 + -2.3 227,574 + -0.5 227,599 + -2.0 227,600 - +1.8 227,647 + -3.2 227,647 + -0.2 227,647 + -1.6 227,648 - -1.2 227,763 + EX31_RS11470 0.17 -2.0 227,763 + EX31_RS11470 0.17 +0.2 227,765 + EX31_RS11470 0.17 -2.6 227,765 + EX31_RS11470 0.17 -3.0 227,765 + EX31_RS11470 0.17 -2.1 227,766 - EX31_RS11470 0.18 -0.8 227,810 - EX31_RS11470 0.25 -0.2 228,057 + EX31_RS11470 0.65 -2.4 228,059 + EX31_RS11470 0.65 -3.1 228,270 + -0.1 228,371 - +0.2 228,371 - +0.4 228,371 - +0.3 228,371 - +0.4 228,392 + +1.0 228,393 - -0.0 228,393 - -1.1 228,416 - -1.2 228,416 - -1.0 228,441 - -1.0 228,441 - -0.2
Or see this region's nucleotide sequence