Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11320 and EX31_RS11325 are separated by 159 nucleotides EX31_RS11325 and EX31_RS11330 are separated by 49 nucleotides EX31_RS11330 and EX31_RS11335 are separated by 431 nucleotides EX31_RS11335 and EX31_RS11340 are separated by 1 nucleotides
EX31_RS11320: EX31_RS11320 - flagellar basal body P-ring formation protein FlgA, at 195,658 to 196,440
_RS11320
EX31_RS11325: EX31_RS11325 - anti-sigma-28 factor FlgM, at 196,600 to 196,902
_RS11325
EX31_RS11330: EX31_RS11330 - flagellar synthesis chaperone protein FlgN, at 196,952 to 197,404
_RS11330
EX31_RS11335: EX31_RS11335 - flagellar protein FlhE, at 197,836 to 198,252
_RS11335
EX31_RS11340: EX31_RS11340 - flagellar biosynthesis protein FlhA, at 198,254 to 200,338
_RS11340
Position (kb) 196
197
198 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 195.953 kb on + strand, within EX31_RS11320 at 195.954 kb on - strand, within EX31_RS11320 at 195.954 kb on - strand, within EX31_RS11320 at 195.967 kb on - strand, within EX31_RS11320 at 196.020 kb on + strand, within EX31_RS11320 at 196.040 kb on + strand, within EX31_RS11320 at 196.040 kb on + strand, within EX31_RS11320 at 196.040 kb on + strand, within EX31_RS11320 at 196.041 kb on - strand, within EX31_RS11320 at 196.069 kb on + strand, within EX31_RS11320 at 196.311 kb on + strand, within EX31_RS11320 at 196.311 kb on + strand, within EX31_RS11320 at 196.311 kb on + strand, within EX31_RS11320 at 196.312 kb on - strand, within EX31_RS11320 at 196.317 kb on - strand, within EX31_RS11320 at 196.436 kb on + strand at 196.436 kb on + strand at 196.438 kb on + strand at 196.438 kb on + strand at 196.438 kb on + strand at 196.438 kb on + strand at 196.438 kb on + strand at 196.444 kb on + strand at 196.478 kb on + strand at 196.478 kb on + strand at 196.518 kb on + strand at 196.518 kb on + strand at 196.518 kb on + strand at 196.599 kb on + strand at 196.599 kb on + strand at 196.614 kb on + strand at 196.614 kb on + strand at 196.614 kb on + strand at 196.614 kb on + strand at 196.614 kb on + strand at 196.885 kb on + strand at 196.885 kb on + strand at 196.940 kb on + strand at 196.940 kb on + strand at 196.943 kb on + strand at 196.944 kb on - strand at 196.978 kb on + strand at 196.978 kb on + strand at 197.018 kb on - strand, within EX31_RS11330 at 197.052 kb on + strand, within EX31_RS11330 at 197.074 kb on + strand, within EX31_RS11330 at 197.074 kb on + strand, within EX31_RS11330 at 197.074 kb on + strand, within EX31_RS11330 at 197.074 kb on + strand, within EX31_RS11330 at 197.074 kb on + strand, within EX31_RS11330 at 197.074 kb on + strand, within EX31_RS11330 at 197.074 kb on + strand, within EX31_RS11330 at 197.074 kb on + strand, within EX31_RS11330 at 197.074 kb on + strand, within EX31_RS11330 at 197.074 kb on + strand, within EX31_RS11330 at 197.074 kb on + strand, within EX31_RS11330 at 197.074 kb on + strand, within EX31_RS11330 at 197.075 kb on - strand, within EX31_RS11330 at 197.075 kb on - strand, within EX31_RS11330 at 197.076 kb on + strand, within EX31_RS11330 at 197.076 kb on + strand, within EX31_RS11330 at 197.076 kb on + strand, within EX31_RS11330 at 197.114 kb on + strand, within EX31_RS11330 at 197.115 kb on - strand, within EX31_RS11330 at 197.221 kb on + strand, within EX31_RS11330 at 197.221 kb on + strand, within EX31_RS11330 at 197.221 kb on + strand, within EX31_RS11330 at 197.221 kb on + strand, within EX31_RS11330 at 197.221 kb on + strand, within EX31_RS11330 at 197.222 kb on - strand, within EX31_RS11330 at 197.222 kb on - strand, within EX31_RS11330 at 197.253 kb on + strand, within EX31_RS11330 at 197.274 kb on + strand, within EX31_RS11330 at 197.274 kb on + strand, within EX31_RS11330 at 197.294 kb on + strand, within EX31_RS11330 at 197.295 kb on - strand, within EX31_RS11330 at 197.296 kb on + strand, within EX31_RS11330 at 197.297 kb on - strand, within EX31_RS11330 at 197.297 kb on - strand, within EX31_RS11330 at 197.441 kb on + strand at 197.441 kb on + strand at 197.442 kb on - strand at 197.477 kb on + strand at 197.483 kb on + strand at 197.483 kb on + strand at 197.491 kb on + strand at 197.583 kb on + strand at 197.583 kb on + strand at 197.584 kb on - strand at 197.584 kb on - strand at 197.584 kb on - strand at 197.584 kb on - strand at 197.597 kb on - strand at 197.631 kb on + strand at 197.631 kb on + strand at 197.632 kb on - strand at 197.649 kb on - strand at 197.649 kb on - strand at 197.697 kb on - strand at 197.697 kb on - strand at 197.697 kb on - strand at 197.713 kb on + strand at 197.714 kb on - strand at 197.742 kb on + strand at 197.743 kb on - strand at 197.816 kb on + strand at 197.816 kb on + strand at 197.819 kb on + strand at 197.819 kb on + strand at 197.820 kb on - strand at 197.820 kb on - strand at 197.820 kb on - strand at 197.837 kb on + strand at 197.843 kb on + strand at 197.843 kb on + strand at 197.844 kb on - strand at 197.923 kb on + strand, within EX31_RS11335 at 197.949 kb on - strand, within EX31_RS11335 at 198.064 kb on + strand, within EX31_RS11335 at 198.065 kb on - strand, within EX31_RS11335 at 198.065 kb on - strand, within EX31_RS11335 at 198.256 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 195,953 + EX31_RS11320 0.38 +0.8 195,954 - EX31_RS11320 0.38 +3.4 195,954 - EX31_RS11320 0.38 -0.5 195,967 - EX31_RS11320 0.39 +0.2 196,020 + EX31_RS11320 0.46 -0.3 196,040 + EX31_RS11320 0.49 -0.5 196,040 + EX31_RS11320 0.49 -0.5 196,040 + EX31_RS11320 0.49 -0.8 196,041 - EX31_RS11320 0.49 +1.8 196,069 + EX31_RS11320 0.52 -1.4 196,311 + EX31_RS11320 0.83 -0.2 196,311 + EX31_RS11320 0.83 +0.1 196,311 + EX31_RS11320 0.83 +0.4 196,312 - EX31_RS11320 0.84 +0.2 196,317 - EX31_RS11320 0.84 +0.5 196,436 + +1.0 196,436 + +1.2 196,438 + +0.1 196,438 + -0.2 196,438 + -0.6 196,438 + -1.2 196,438 + +0.2 196,444 + +0.8 196,478 + +1.2 196,478 + -1.9 196,518 + +0.6 196,518 + +1.5 196,518 + +1.4 196,599 + +0.3 196,599 + -0.9 196,614 + -1.1 196,614 + +0.8 196,614 + -1.5 196,614 + -0.9 196,614 + -3.6 196,885 + +0.4 196,885 + -1.0 196,940 + +0.6 196,940 + +0.2 196,943 + +1.8 196,944 - -0.5 196,978 + -1.2 196,978 + -1.2 197,018 - EX31_RS11330 0.15 -1.5 197,052 + EX31_RS11330 0.22 +0.6 197,074 + EX31_RS11330 0.27 -0.6 197,074 + EX31_RS11330 0.27 -0.3 197,074 + EX31_RS11330 0.27 -2.0 197,074 + EX31_RS11330 0.27 -0.6 197,074 + EX31_RS11330 0.27 -0.1 197,074 + EX31_RS11330 0.27 -0.3 197,074 + EX31_RS11330 0.27 -0.4 197,074 + EX31_RS11330 0.27 +0.4 197,074 + EX31_RS11330 0.27 -2.9 197,074 + EX31_RS11330 0.27 +0.1 197,074 + EX31_RS11330 0.27 -1.3 197,074 + EX31_RS11330 0.27 -0.1 197,075 - EX31_RS11330 0.27 +0.6 197,075 - EX31_RS11330 0.27 +1.2 197,076 + EX31_RS11330 0.27 +0.6 197,076 + EX31_RS11330 0.27 -0.3 197,076 + EX31_RS11330 0.27 +0.1 197,114 + EX31_RS11330 0.36 +1.8 197,115 - EX31_RS11330 0.36 +0.2 197,221 + EX31_RS11330 0.59 +2.4 197,221 + EX31_RS11330 0.59 -0.5 197,221 + EX31_RS11330 0.59 -0.3 197,221 + EX31_RS11330 0.59 -0.5 197,221 + EX31_RS11330 0.59 -2.7 197,222 - EX31_RS11330 0.60 -0.5 197,222 - EX31_RS11330 0.60 +1.8 197,253 + EX31_RS11330 0.66 -2.1 197,274 + EX31_RS11330 0.71 -0.8 197,274 + EX31_RS11330 0.71 -1.1 197,294 + EX31_RS11330 0.75 -0.3 197,295 - EX31_RS11330 0.76 +0.2 197,296 + EX31_RS11330 0.76 -2.5 197,297 - EX31_RS11330 0.76 +1.2 197,297 - EX31_RS11330 0.76 -0.2 197,441 + +1.1 197,441 + -2.4 197,442 - +0.8 197,477 + +1.8 197,483 + +1.1 197,483 + +0.4 197,491 + +4.4 197,583 + +0.2 197,583 + +1.2 197,584 - +1.0 197,584 - -0.6 197,584 - +2.3 197,584 - -0.4 197,597 - -2.3 197,631 + -1.1 197,631 + +2.1 197,632 - -0.2 197,649 - +0.4 197,649 - -1.4 197,697 - -0.3 197,697 - -0.0 197,697 - -0.9 197,713 + -1.5 197,714 - -0.9 197,742 + -2.2 197,743 - +0.7 197,816 + +0.5 197,816 + +0.2 197,819 + -1.0 197,819 + -0.2 197,820 - +0.5 197,820 - +1.8 197,820 - +0.4 197,837 + -0.4 197,843 + -0.1 197,843 + -1.6 197,844 - -0.3 197,923 + EX31_RS11335 0.21 +1.2 197,949 - EX31_RS11335 0.27 +1.8 198,064 + EX31_RS11335 0.55 +0.5 198,065 - EX31_RS11335 0.55 +1.4 198,065 - EX31_RS11335 0.55 +0.2 198,256 - -0.1
Or see this region's nucleotide sequence