Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11265 and EX31_RS11270 are separated by 27 nucleotides EX31_RS11270 and EX31_RS11275 are separated by 140 nucleotides
EX31_RS11265: EX31_RS11265 - flagellar hook-filament junction protein FlgL, at 185,409 to 186,371
_RS11265
EX31_RS11270: EX31_RS11270 - flagellar hook-associated protein FlgK, at 186,399 to 188,051
_RS11270
EX31_RS11275: EX31_RS11275 - flagellar assembly peptidoglycan hydrolase FlgJ, at 188,192 to 189,142
_RS11275
Position (kb) 186
187
188
189 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 185.407 kb on + strand at 185.408 kb on - strand at 185.408 kb on - strand at 185.435 kb on + strand at 185.435 kb on + strand at 185.435 kb on + strand at 185.436 kb on - strand at 185.436 kb on - strand at 185.436 kb on - strand at 185.436 kb on - strand at 185.452 kb on + strand at 185.453 kb on - strand at 185.459 kb on + strand at 185.459 kb on + strand at 185.459 kb on + strand at 185.460 kb on - strand at 185.460 kb on - strand at 185.460 kb on - strand at 185.460 kb on - strand at 185.460 kb on - strand at 185.460 kb on - strand at 185.460 kb on - strand at 185.460 kb on - strand at 185.461 kb on + strand at 185.461 kb on + strand at 185.461 kb on + strand at 185.461 kb on + strand at 185.461 kb on + strand at 185.461 kb on + strand at 185.461 kb on + strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.494 kb on + strand at 185.494 kb on + strand at 185.495 kb on - strand at 185.495 kb on - strand at 185.652 kb on - strand, within EX31_RS11265 at 185.696 kb on + strand, within EX31_RS11265 at 185.760 kb on + strand, within EX31_RS11265 at 185.865 kb on - strand, within EX31_RS11265 at 185.879 kb on + strand, within EX31_RS11265 at 185.882 kb on + strand, within EX31_RS11265 at 185.882 kb on + strand, within EX31_RS11265 at 185.883 kb on - strand, within EX31_RS11265 at 185.903 kb on - strand, within EX31_RS11265 at 185.933 kb on + strand, within EX31_RS11265 at 185.933 kb on + strand, within EX31_RS11265 at 185.933 kb on + strand, within EX31_RS11265 at 185.933 kb on + strand, within EX31_RS11265 at 185.934 kb on - strand, within EX31_RS11265 at 185.934 kb on - strand, within EX31_RS11265 at 185.934 kb on - strand, within EX31_RS11265 at 185.934 kb on - strand, within EX31_RS11265 at 185.934 kb on - strand, within EX31_RS11265 at 185.934 kb on - strand, within EX31_RS11265 at 185.934 kb on - strand, within EX31_RS11265 at 185.945 kb on + strand, within EX31_RS11265 at 185.945 kb on + strand, within EX31_RS11265 at 186.155 kb on + strand, within EX31_RS11265 at 186.156 kb on - strand, within EX31_RS11265 at 186.156 kb on - strand, within EX31_RS11265 at 186.288 kb on + strand at 186.288 kb on + strand at 186.347 kb on - strand at 186.347 kb on - strand at 186.347 kb on - strand at 186.347 kb on - strand at 186.347 kb on - strand at 186.354 kb on - strand at 186.362 kb on + strand at 186.362 kb on + strand at 186.363 kb on - strand at 186.467 kb on - strand at 186.467 kb on - strand at 186.467 kb on - strand at 186.467 kb on - strand at 186.467 kb on - strand at 186.467 kb on - strand at 186.467 kb on - strand at 186.470 kb on - strand at 186.470 kb on - strand at 186.470 kb on - strand at 186.478 kb on + strand at 186.497 kb on - strand at 186.524 kb on + strand at 186.611 kb on + strand, within EX31_RS11270 at 186.611 kb on + strand, within EX31_RS11270 at 186.611 kb on + strand, within EX31_RS11270 at 186.611 kb on + strand, within EX31_RS11270 at 186.611 kb on + strand, within EX31_RS11270 at 186.611 kb on + strand, within EX31_RS11270 at 186.612 kb on - strand, within EX31_RS11270 at 186.612 kb on - strand, within EX31_RS11270 at 186.615 kb on + strand, within EX31_RS11270 at 186.615 kb on + strand, within EX31_RS11270 at 186.616 kb on - strand, within EX31_RS11270 at 186.616 kb on - strand, within EX31_RS11270 at 186.616 kb on - strand, within EX31_RS11270 at 186.822 kb on - strand, within EX31_RS11270 at 186.933 kb on + strand, within EX31_RS11270 at 186.934 kb on - strand, within EX31_RS11270 at 186.955 kb on + strand, within EX31_RS11270 at 186.956 kb on - strand, within EX31_RS11270 at 186.963 kb on - strand, within EX31_RS11270 at 186.967 kb on + strand, within EX31_RS11270 at 186.967 kb on + strand, within EX31_RS11270 at 186.968 kb on - strand, within EX31_RS11270 at 186.999 kb on - strand, within EX31_RS11270 at 187.000 kb on + strand, within EX31_RS11270 at 187.000 kb on + strand, within EX31_RS11270 at 187.001 kb on - strand, within EX31_RS11270 at 187.001 kb on - strand, within EX31_RS11270 at 187.001 kb on - strand, within EX31_RS11270 at 187.001 kb on - strand, within EX31_RS11270 at 187.374 kb on - strand, within EX31_RS11270 at 187.374 kb on - strand, within EX31_RS11270 at 187.374 kb on - strand, within EX31_RS11270 at 187.374 kb on - strand, within EX31_RS11270 at 187.378 kb on + strand, within EX31_RS11270 at 187.378 kb on + strand, within EX31_RS11270 at 187.378 kb on + strand, within EX31_RS11270 at 187.378 kb on + strand, within EX31_RS11270 at 187.378 kb on + strand, within EX31_RS11270 at 187.378 kb on + strand, within EX31_RS11270 at 187.379 kb on - strand, within EX31_RS11270 at 187.379 kb on - strand, within EX31_RS11270 at 187.382 kb on + strand, within EX31_RS11270 at 187.382 kb on + strand, within EX31_RS11270 at 187.382 kb on + strand, within EX31_RS11270 at 187.382 kb on + strand, within EX31_RS11270 at 187.467 kb on - strand, within EX31_RS11270 at 187.467 kb on - strand, within EX31_RS11270 at 187.534 kb on + strand, within EX31_RS11270 at 187.534 kb on + strand, within EX31_RS11270 at 187.534 kb on + strand, within EX31_RS11270 at 187.534 kb on + strand, within EX31_RS11270 at 187.534 kb on + strand, within EX31_RS11270 at 187.534 kb on + strand, within EX31_RS11270 at 187.535 kb on - strand, within EX31_RS11270 at 187.535 kb on - strand, within EX31_RS11270 at 187.535 kb on - strand, within EX31_RS11270 at 187.535 kb on - strand, within EX31_RS11270 at 187.535 kb on - strand, within EX31_RS11270 at 187.600 kb on + strand, within EX31_RS11270 at 187.601 kb on - strand, within EX31_RS11270 at 187.850 kb on - strand, within EX31_RS11270 at 187.890 kb on - strand at 187.892 kb on - strand at 187.905 kb on - strand at 187.917 kb on - strand at 187.926 kb on - strand at 187.947 kb on - strand at 187.949 kb on - strand at 188.033 kb on + strand at 188.034 kb on - strand at 188.037 kb on + strand at 188.038 kb on - strand at 188.038 kb on - strand at 188.038 kb on - strand at 188.082 kb on - strand at 188.091 kb on + strand at 188.092 kb on - strand at 188.092 kb on - strand at 188.095 kb on + strand at 188.095 kb on + strand at 188.200 kb on + strand at 188.200 kb on + strand at 188.220 kb on + strand at 188.286 kb on + strand at 188.287 kb on - strand, within EX31_RS11275 at 188.344 kb on + strand, within EX31_RS11275 at 188.344 kb on + strand, within EX31_RS11275 at 188.384 kb on - strand, within EX31_RS11275 at 188.397 kb on + strand, within EX31_RS11275 at 188.398 kb on - strand, within EX31_RS11275 at 188.398 kb on - strand, within EX31_RS11275 at 188.398 kb on - strand, within EX31_RS11275 at 188.418 kb on + strand, within EX31_RS11275 at 188.419 kb on - strand, within EX31_RS11275 at 188.419 kb on - strand, within EX31_RS11275 at 188.419 kb on - strand, within EX31_RS11275 at 188.427 kb on + strand, within EX31_RS11275 at 188.427 kb on + strand, within EX31_RS11275 at 188.470 kb on + strand, within EX31_RS11275 at 188.471 kb on - strand, within EX31_RS11275 at 188.471 kb on - strand, within EX31_RS11275 at 188.471 kb on - strand, within EX31_RS11275 at 188.642 kb on - strand, within EX31_RS11275 at 188.755 kb on - strand, within EX31_RS11275 at 188.926 kb on - strand, within EX31_RS11275 at 188.926 kb on - strand, within EX31_RS11275 at 188.926 kb on - strand, within EX31_RS11275 at 188.935 kb on + strand, within EX31_RS11275
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 185,407 + -1.0 185,408 - -0.2 185,408 - +0.5 185,435 + -0.5 185,435 + -0.4 185,435 + +1.0 185,436 - -0.5 185,436 - +0.6 185,436 - -0.8 185,436 - -1.0 185,452 + +2.8 185,453 - -1.2 185,459 + -0.9 185,459 + -0.7 185,459 + +0.5 185,460 - +1.1 185,460 - -1.4 185,460 - +1.1 185,460 - +0.2 185,460 - -0.2 185,460 - +0.8 185,460 - -0.5 185,460 - -2.0 185,461 + +1.0 185,461 + +0.1 185,461 + -0.2 185,461 + +2.8 185,461 + +0.4 185,461 + +0.2 185,461 + -2.3 185,462 - -0.8 185,462 - -1.9 185,462 - +0.2 185,462 - +1.4 185,462 - -1.4 185,462 - -0.6 185,462 - +0.2 185,462 - +0.6 185,462 - -0.5 185,494 + -0.9 185,494 + -2.4 185,495 - +0.2 185,495 - +1.6 185,652 - EX31_RS11265 0.25 +1.6 185,696 + EX31_RS11265 0.30 -1.0 185,760 + EX31_RS11265 0.36 -0.7 185,865 - EX31_RS11265 0.47 -1.2 185,879 + EX31_RS11265 0.49 -2.8 185,882 + EX31_RS11265 0.49 +0.8 185,882 + EX31_RS11265 0.49 +1.4 185,883 - EX31_RS11265 0.49 +0.8 185,903 - EX31_RS11265 0.51 -0.5 185,933 + EX31_RS11265 0.54 -1.0 185,933 + EX31_RS11265 0.54 +0.7 185,933 + EX31_RS11265 0.54 +0.5 185,933 + EX31_RS11265 0.54 +0.2 185,934 - EX31_RS11265 0.55 -0.4 185,934 - EX31_RS11265 0.55 -0.8 185,934 - EX31_RS11265 0.55 +0.6 185,934 - EX31_RS11265 0.55 +2.2 185,934 - EX31_RS11265 0.55 -0.3 185,934 - EX31_RS11265 0.55 -0.4 185,934 - EX31_RS11265 0.55 -2.7 185,945 + EX31_RS11265 0.56 -1.9 185,945 + EX31_RS11265 0.56 -0.8 186,155 + EX31_RS11265 0.77 -1.4 186,156 - EX31_RS11265 0.78 -1.4 186,156 - EX31_RS11265 0.78 -1.0 186,288 + -0.6 186,288 + -0.1 186,347 - -1.0 186,347 - -1.6 186,347 - +1.5 186,347 - -0.5 186,347 - +0.2 186,354 - -1.2 186,362 + -0.3 186,362 + -1.2 186,363 - -0.8 186,467 - +0.0 186,467 - +0.8 186,467 - -1.0 186,467 - -0.5 186,467 - -0.3 186,467 - -1.0 186,467 - -1.6 186,470 - +0.2 186,470 - -1.0 186,470 - +0.8 186,478 + +1.8 186,497 - +1.0 186,524 + +0.2 186,611 + EX31_RS11270 0.13 -1.6 186,611 + EX31_RS11270 0.13 -1.0 186,611 + EX31_RS11270 0.13 -0.5 186,611 + EX31_RS11270 0.13 -1.8 186,611 + EX31_RS11270 0.13 +1.8 186,611 + EX31_RS11270 0.13 -1.0 186,612 - EX31_RS11270 0.13 +0.2 186,612 - EX31_RS11270 0.13 -0.2 186,615 + EX31_RS11270 0.13 -0.4 186,615 + EX31_RS11270 0.13 +1.0 186,616 - EX31_RS11270 0.13 +0.2 186,616 - EX31_RS11270 0.13 -2.7 186,616 - EX31_RS11270 0.13 +0.6 186,822 - EX31_RS11270 0.26 +0.4 186,933 + EX31_RS11270 0.32 -2.6 186,934 - EX31_RS11270 0.32 -1.4 186,955 + EX31_RS11270 0.34 +1.5 186,956 - EX31_RS11270 0.34 -0.2 186,963 - EX31_RS11270 0.34 -0.3 186,967 + EX31_RS11270 0.34 -1.5 186,967 + EX31_RS11270 0.34 +0.7 186,968 - EX31_RS11270 0.34 -1.9 186,999 - EX31_RS11270 0.36 -0.6 187,000 + EX31_RS11270 0.36 +0.7 187,000 + EX31_RS11270 0.36 -0.4 187,001 - EX31_RS11270 0.36 -0.4 187,001 - EX31_RS11270 0.36 -1.8 187,001 - EX31_RS11270 0.36 -1.6 187,001 - EX31_RS11270 0.36 -1.5 187,374 - EX31_RS11270 0.59 +0.2 187,374 - EX31_RS11270 0.59 +1.1 187,374 - EX31_RS11270 0.59 -1.9 187,374 - EX31_RS11270 0.59 +0.1 187,378 + EX31_RS11270 0.59 +0.1 187,378 + EX31_RS11270 0.59 -1.8 187,378 + EX31_RS11270 0.59 +0.8 187,378 + EX31_RS11270 0.59 -1.6 187,378 + EX31_RS11270 0.59 +1.1 187,378 + EX31_RS11270 0.59 -1.1 187,379 - EX31_RS11270 0.59 -1.2 187,379 - EX31_RS11270 0.59 -0.8 187,382 + EX31_RS11270 0.59 +1.3 187,382 + EX31_RS11270 0.59 -0.3 187,382 + EX31_RS11270 0.59 +0.8 187,382 + EX31_RS11270 0.59 +0.0 187,467 - EX31_RS11270 0.65 -0.8 187,467 - EX31_RS11270 0.65 -0.0 187,534 + EX31_RS11270 0.69 +1.2 187,534 + EX31_RS11270 0.69 +0.2 187,534 + EX31_RS11270 0.69 -0.4 187,534 + EX31_RS11270 0.69 -0.4 187,534 + EX31_RS11270 0.69 -0.5 187,534 + EX31_RS11270 0.69 -0.7 187,535 - EX31_RS11270 0.69 -1.2 187,535 - EX31_RS11270 0.69 -0.1 187,535 - EX31_RS11270 0.69 -1.3 187,535 - EX31_RS11270 0.69 +0.4 187,535 - EX31_RS11270 0.69 +0.5 187,600 + EX31_RS11270 0.73 +0.3 187,601 - EX31_RS11270 0.73 +0.5 187,850 - EX31_RS11270 0.88 +1.5 187,890 - -1.1 187,892 - -1.1 187,905 - +0.8 187,917 - +0.1 187,926 - +0.4 187,947 - -0.2 187,949 - +0.5 188,033 + -0.1 188,034 - +0.5 188,037 + +2.4 188,038 - +0.2 188,038 - +0.3 188,038 - -0.8 188,082 - +0.5 188,091 + +1.1 188,092 - -0.4 188,092 - -0.3 188,095 + -2.2 188,095 + -0.4 188,200 + +0.0 188,200 + -1.0 188,220 + +0.5 188,286 + +1.6 188,287 - EX31_RS11275 0.10 -0.7 188,344 + EX31_RS11275 0.16 +0.8 188,344 + EX31_RS11275 0.16 +0.3 188,384 - EX31_RS11275 0.20 +1.8 188,397 + EX31_RS11275 0.22 -0.5 188,398 - EX31_RS11275 0.22 -0.4 188,398 - EX31_RS11275 0.22 -0.4 188,398 - EX31_RS11275 0.22 +0.8 188,418 + EX31_RS11275 0.24 +1.1 188,419 - EX31_RS11275 0.24 +0.2 188,419 - EX31_RS11275 0.24 -0.8 188,419 - EX31_RS11275 0.24 +0.2 188,427 + EX31_RS11275 0.25 -0.3 188,427 + EX31_RS11275 0.25 +0.8 188,470 + EX31_RS11275 0.29 +3.0 188,471 - EX31_RS11275 0.29 -0.1 188,471 - EX31_RS11275 0.29 +2.1 188,471 - EX31_RS11275 0.29 -0.5 188,642 - EX31_RS11275 0.47 +0.8 188,755 - EX31_RS11275 0.59 -0.1 188,926 - EX31_RS11275 0.77 +1.2 188,926 - EX31_RS11275 0.77 -0.2 188,926 - EX31_RS11275 0.77 +0.3 188,935 + EX31_RS11275 0.78 +1.6
Or see this region's nucleotide sequence