Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09950 and EX31_RS09955 are separated by 490 nucleotides EX31_RS09955 and EX31_RS09960 are separated by 1 nucleotides EX31_RS09960 and EX31_RS09965 are separated by 11 nucleotides
EX31_RS09950: EX31_RS09950 - PHP domain-containing protein, at 198,570 to 199,493
_RS09950
EX31_RS09955: EX31_RS09955 - anthranilate synthase component 1, at 199,984 to 201,546
_RS09955
EX31_RS09960: EX31_RS09960 - glutamine amidotransferase, at 201,548 to 202,126
_RS09960
EX31_RS09965: EX31_RS09965 - anthranilate phosphoribosyltransferase, at 202,138 to 203,151
_RS09965
Position (kb) 199
200
201
202 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 199.063 kb on - strand, within EX31_RS09950 at 199.141 kb on + strand, within EX31_RS09950 at 199.142 kb on - strand, within EX31_RS09950 at 199.142 kb on - strand, within EX31_RS09950 at 199.158 kb on + strand, within EX31_RS09950 at 199.159 kb on - strand, within EX31_RS09950 at 199.205 kb on + strand, within EX31_RS09950 at 199.206 kb on - strand, within EX31_RS09950 at 199.252 kb on - strand, within EX31_RS09950 at 199.357 kb on - strand, within EX31_RS09950 at 199.390 kb on + strand, within EX31_RS09950 at 199.391 kb on - strand, within EX31_RS09950 at 199.409 kb on + strand at 199.410 kb on - strand at 199.410 kb on - strand at 199.426 kb on + strand at 199.433 kb on + strand at 199.434 kb on - strand at 199.434 kb on - strand at 199.533 kb on + strand at 199.533 kb on + strand at 199.533 kb on + strand at 199.534 kb on - strand at 199.534 kb on - strand at 199.534 kb on - strand at 199.534 kb on - strand at 199.534 kb on - strand at 199.534 kb on - strand at 199.538 kb on + strand at 199.595 kb on - strand at 199.602 kb on + strand at 199.602 kb on + strand at 199.603 kb on - strand at 199.603 kb on - strand at 199.603 kb on - strand at 199.603 kb on - strand at 199.603 kb on - strand at 199.604 kb on + strand at 199.604 kb on + strand at 199.604 kb on + strand at 199.653 kb on + strand at 199.691 kb on + strand at 199.711 kb on + strand at 199.711 kb on + strand at 199.754 kb on - strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.770 kb on - strand at 199.809 kb on + strand at 199.809 kb on + strand at 199.809 kb on + strand at 199.839 kb on - strand at 199.860 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.871 kb on - strand at 199.871 kb on - strand at 199.953 kb on + strand at 199.953 kb on + strand at 199.989 kb on + strand at 199.990 kb on - strand at 199.990 kb on - strand at 199.990 kb on - strand at 200.006 kb on + strand at 200.222 kb on + strand, within EX31_RS09955 at 200.474 kb on + strand, within EX31_RS09955 at 200.475 kb on - strand, within EX31_RS09955 at 200.509 kb on + strand, within EX31_RS09955 at 200.509 kb on + strand, within EX31_RS09955 at 200.510 kb on - strand, within EX31_RS09955 at 200.510 kb on - strand, within EX31_RS09955 at 200.532 kb on - strand, within EX31_RS09955 at 200.640 kb on - strand, within EX31_RS09955 at 200.640 kb on - strand, within EX31_RS09955 at 200.729 kb on + strand, within EX31_RS09955 at 200.729 kb on + strand, within EX31_RS09955 at 200.729 kb on + strand, within EX31_RS09955 at 200.842 kb on - strand, within EX31_RS09955 at 200.842 kb on - strand, within EX31_RS09955 at 200.858 kb on - strand, within EX31_RS09955 at 200.858 kb on - strand, within EX31_RS09955 at 200.858 kb on - strand, within EX31_RS09955 at 200.859 kb on + strand, within EX31_RS09955 at 200.859 kb on + strand, within EX31_RS09955 at 200.859 kb on + strand, within EX31_RS09955 at 200.859 kb on + strand, within EX31_RS09955 at 200.859 kb on + strand, within EX31_RS09955 at 200.859 kb on + strand, within EX31_RS09955 at 200.859 kb on + strand, within EX31_RS09955 at 200.859 kb on + strand, within EX31_RS09955 at 200.859 kb on + strand, within EX31_RS09955 at 200.859 kb on + strand, within EX31_RS09955 at 200.859 kb on + strand, within EX31_RS09955 at 200.860 kb on - strand, within EX31_RS09955 at 200.860 kb on - strand, within EX31_RS09955 at 200.860 kb on - strand, within EX31_RS09955 at 200.860 kb on - strand, within EX31_RS09955 at 200.923 kb on + strand, within EX31_RS09955 at 200.954 kb on - strand, within EX31_RS09955 at 201.052 kb on - strand, within EX31_RS09955 at 201.108 kb on + strand, within EX31_RS09955 at 201.109 kb on - strand, within EX31_RS09955 at 201.147 kb on + strand, within EX31_RS09955 at 201.222 kb on - strand, within EX31_RS09955 at 201.222 kb on - strand, within EX31_RS09955 at 201.222 kb on - strand, within EX31_RS09955 at 201.232 kb on + strand, within EX31_RS09955 at 201.233 kb on - strand, within EX31_RS09955 at 201.346 kb on + strand, within EX31_RS09955 at 201.347 kb on - strand, within EX31_RS09955 at 201.368 kb on + strand, within EX31_RS09955 at 201.368 kb on + strand, within EX31_RS09955 at 201.372 kb on + strand, within EX31_RS09955 at 201.398 kb on - strand at 201.398 kb on - strand at 201.398 kb on - strand at 201.483 kb on + strand at 201.486 kb on + strand at 201.486 kb on + strand at 201.540 kb on - strand at 201.544 kb on + strand at 201.544 kb on + strand at 201.545 kb on - strand at 201.545 kb on - strand at 201.562 kb on - strand at 201.562 kb on - strand at 201.562 kb on - strand at 201.562 kb on - strand at 201.587 kb on - strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.591 kb on - strand at 201.591 kb on - strand at 201.591 kb on - strand at 201.591 kb on - strand at 201.591 kb on - strand at 201.625 kb on + strand, within EX31_RS09960 at 201.626 kb on - strand, within EX31_RS09960 at 201.626 kb on - strand, within EX31_RS09960 at 201.626 kb on - strand, within EX31_RS09960 at 201.626 kb on - strand, within EX31_RS09960 at 201.626 kb on - strand, within EX31_RS09960 at 201.626 kb on - strand, within EX31_RS09960 at 201.638 kb on + strand, within EX31_RS09960 at 201.827 kb on + strand, within EX31_RS09960 at 201.880 kb on + strand, within EX31_RS09960 at 201.880 kb on + strand, within EX31_RS09960 at 201.880 kb on + strand, within EX31_RS09960 at 201.880 kb on + strand, within EX31_RS09960 at 201.881 kb on - strand, within EX31_RS09960 at 201.883 kb on + strand, within EX31_RS09960 at 201.883 kb on + strand, within EX31_RS09960 at 202.004 kb on - strand, within EX31_RS09960 at 202.017 kb on + strand, within EX31_RS09960 at 202.018 kb on - strand, within EX31_RS09960 at 202.152 kb on + strand at 202.153 kb on - strand at 202.217 kb on + strand at 202.218 kb on - strand at 202.218 kb on - strand at 202.419 kb on + strand, within EX31_RS09965 at 202.419 kb on + strand, within EX31_RS09965 at 202.420 kb on - strand, within EX31_RS09965 at 202.518 kb on - strand, within EX31_RS09965
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 199,063 - EX31_RS09950 0.53 -0.5 199,141 + EX31_RS09950 0.62 +0.1 199,142 - EX31_RS09950 0.62 +0.6 199,142 - EX31_RS09950 0.62 -0.2 199,158 + EX31_RS09950 0.64 +0.5 199,159 - EX31_RS09950 0.64 -0.4 199,205 + EX31_RS09950 0.69 +2.6 199,206 - EX31_RS09950 0.69 +0.7 199,252 - EX31_RS09950 0.74 +0.5 199,357 - EX31_RS09950 0.85 -0.1 199,390 + EX31_RS09950 0.89 +0.2 199,391 - EX31_RS09950 0.89 -0.4 199,409 + +1.1 199,410 - +1.3 199,410 - -1.7 199,426 + -2.1 199,433 + -0.4 199,434 - -0.2 199,434 - +1.5 199,533 + -0.6 199,533 + -0.9 199,533 + -1.3 199,534 - +0.7 199,534 - +0.9 199,534 - -0.1 199,534 - +0.1 199,534 - -0.9 199,534 - -1.0 199,538 + +0.9 199,595 - -0.0 199,602 + +0.3 199,602 + +0.9 199,603 - -0.2 199,603 - +1.5 199,603 - -0.4 199,603 - -0.0 199,603 - +0.1 199,604 + +1.6 199,604 + +0.1 199,604 + -0.6 199,653 + +0.4 199,691 + -1.4 199,711 + -0.1 199,711 + +0.4 199,754 - -1.3 199,769 + -1.1 199,769 + -1.9 199,769 + +0.3 199,770 - -0.3 199,809 + +0.5 199,809 + +0.9 199,809 + -0.0 199,839 - +0.1 199,860 + -2.0 199,870 + +1.2 199,870 + -0.6 199,870 + +1.3 199,870 + +0.5 199,870 + -0.8 199,870 + -1.1 199,870 + +1.7 199,871 - -0.1 199,871 - -2.1 199,953 + -1.0 199,953 + -0.1 199,989 + -2.3 199,990 - -0.3 199,990 - -2.5 199,990 - +0.1 200,006 + -2.2 200,222 + EX31_RS09955 0.15 -1.9 200,474 + EX31_RS09955 0.31 +0.1 200,475 - EX31_RS09955 0.31 -2.4 200,509 + EX31_RS09955 0.34 -1.7 200,509 + EX31_RS09955 0.34 -2.3 200,510 - EX31_RS09955 0.34 -2.5 200,510 - EX31_RS09955 0.34 -2.9 200,532 - EX31_RS09955 0.35 -1.7 200,640 - EX31_RS09955 0.42 -1.4 200,640 - EX31_RS09955 0.42 -1.1 200,729 + EX31_RS09955 0.48 +0.7 200,729 + EX31_RS09955 0.48 -1.1 200,729 + EX31_RS09955 0.48 -1.9 200,842 - EX31_RS09955 0.55 +0.1 200,842 - EX31_RS09955 0.55 -2.2 200,858 - EX31_RS09955 0.56 -1.4 200,858 - EX31_RS09955 0.56 -1.1 200,858 - EX31_RS09955 0.56 -1.9 200,859 + EX31_RS09955 0.56 -1.9 200,859 + EX31_RS09955 0.56 -2.4 200,859 + EX31_RS09955 0.56 -2.4 200,859 + EX31_RS09955 0.56 -1.6 200,859 + EX31_RS09955 0.56 -1.4 200,859 + EX31_RS09955 0.56 -1.4 200,859 + EX31_RS09955 0.56 -2.6 200,859 + EX31_RS09955 0.56 -1.1 200,859 + EX31_RS09955 0.56 -2.7 200,859 + EX31_RS09955 0.56 -1.4 200,859 + EX31_RS09955 0.56 -2.1 200,860 - EX31_RS09955 0.56 -1.9 200,860 - EX31_RS09955 0.56 -1.4 200,860 - EX31_RS09955 0.56 -0.6 200,860 - EX31_RS09955 0.56 -2.6 200,923 + EX31_RS09955 0.60 -1.3 200,954 - EX31_RS09955 0.62 -2.5 201,052 - EX31_RS09955 0.68 -2.2 201,108 + EX31_RS09955 0.72 -1.3 201,109 - EX31_RS09955 0.72 -0.6 201,147 + EX31_RS09955 0.74 -0.6 201,222 - EX31_RS09955 0.79 -2.0 201,222 - EX31_RS09955 0.79 +0.1 201,222 - EX31_RS09955 0.79 -2.0 201,232 + EX31_RS09955 0.80 -2.7 201,233 - EX31_RS09955 0.80 -2.3 201,346 + EX31_RS09955 0.87 -2.0 201,347 - EX31_RS09955 0.87 -2.6 201,368 + EX31_RS09955 0.89 -1.9 201,368 + EX31_RS09955 0.89 -2.0 201,372 + EX31_RS09955 0.89 -0.9 201,398 - -1.3 201,398 - -2.2 201,398 - -1.3 201,483 + -2.4 201,486 + +0.7 201,486 + -2.1 201,540 - -1.3 201,544 + -1.9 201,544 + -2.3 201,545 - -0.3 201,545 - -0.6 201,562 - -2.2 201,562 - -0.6 201,562 - -2.9 201,562 - -2.4 201,587 - -1.7 201,590 + -3.5 201,590 + -3.7 201,590 + -1.1 201,590 + -1.7 201,590 + -2.8 201,590 + -2.2 201,590 + -1.3 201,590 + -3.7 201,590 + -2.7 201,590 + -0.3 201,590 + -1.3 201,591 - -2.4 201,591 - -2.7 201,591 - -2.3 201,591 - -3.0 201,591 - -2.4 201,625 + EX31_RS09960 0.13 -1.1 201,626 - EX31_RS09960 0.13 +0.7 201,626 - EX31_RS09960 0.13 -0.3 201,626 - EX31_RS09960 0.13 -3.5 201,626 - EX31_RS09960 0.13 -1.4 201,626 - EX31_RS09960 0.13 -1.9 201,626 - EX31_RS09960 0.13 -1.9 201,638 + EX31_RS09960 0.16 -0.7 201,827 + EX31_RS09960 0.48 -0.9 201,880 + EX31_RS09960 0.57 -1.1 201,880 + EX31_RS09960 0.57 -2.7 201,880 + EX31_RS09960 0.57 -1.4 201,880 + EX31_RS09960 0.57 -1.3 201,881 - EX31_RS09960 0.58 -1.4 201,883 + EX31_RS09960 0.58 -4.1 201,883 + EX31_RS09960 0.58 -2.4 202,004 - EX31_RS09960 0.79 -1.1 202,017 + EX31_RS09960 0.81 -0.9 202,018 - EX31_RS09960 0.81 -3.4 202,152 + -2.1 202,153 - -2.2 202,217 + -1.7 202,218 - -2.4 202,218 - -1.6 202,419 + EX31_RS09965 0.28 +0.1 202,419 + EX31_RS09965 0.28 -2.6 202,420 - EX31_RS09965 0.28 +1.7 202,518 - EX31_RS09965 0.37 -2.3
Or see this region's nucleotide sequence