Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09710 and EX31_RS09715 are separated by 156 nucleotides EX31_RS09715 and EX31_RS09720 overlap by 4 nucleotides
EX31_RS09710: EX31_RS09710 - LysR family transcriptional regulator, at 142,241 to 143,164
_RS09710
EX31_RS09715: EX31_RS09715 - M20 family metallopeptidase, at 143,321 to 144,742
_RS09715
EX31_RS09720: EX31_RS09720 - MFS transporter, at 144,739 to 146,022
_RS09720
Position (kb)
143
144
145 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 142.364 kb on - strand, within EX31_RS09710 at 142.364 kb on - strand, within EX31_RS09710 at 142.364 kb on - strand, within EX31_RS09710 at 142.364 kb on - strand, within EX31_RS09710 at 142.385 kb on - strand, within EX31_RS09710 at 142.425 kb on + strand, within EX31_RS09710 at 142.425 kb on + strand, within EX31_RS09710 at 142.426 kb on - strand, within EX31_RS09710 at 142.426 kb on - strand, within EX31_RS09710 at 142.429 kb on + strand, within EX31_RS09710 at 142.429 kb on + strand, within EX31_RS09710 at 143.038 kb on + strand, within EX31_RS09710 at 143.091 kb on - strand at 143.091 kb on - strand at 143.180 kb on + strand at 143.180 kb on + strand at 143.180 kb on + strand at 143.199 kb on + strand at 143.200 kb on - strand at 143.234 kb on - strand at 143.288 kb on + strand at 143.288 kb on + strand at 143.288 kb on + strand at 143.288 kb on + strand at 143.288 kb on + strand at 143.288 kb on + strand at 143.288 kb on + strand at 143.288 kb on + strand at 143.288 kb on + strand at 143.289 kb on - strand at 143.289 kb on - strand at 143.375 kb on + strand at 143.376 kb on - strand at 143.404 kb on + strand at 143.404 kb on + strand at 143.454 kb on - strand at 143.551 kb on + strand, within EX31_RS09715 at 143.552 kb on - strand, within EX31_RS09715 at 143.584 kb on - strand, within EX31_RS09715 at 143.722 kb on + strand, within EX31_RS09715 at 143.722 kb on + strand, within EX31_RS09715 at 143.725 kb on + strand, within EX31_RS09715 at 143.982 kb on - strand, within EX31_RS09715 at 144.072 kb on + strand, within EX31_RS09715 at 144.089 kb on - strand, within EX31_RS09715 at 144.215 kb on + strand, within EX31_RS09715 at 144.215 kb on + strand, within EX31_RS09715 at 144.216 kb on - strand, within EX31_RS09715 at 144.216 kb on - strand, within EX31_RS09715 at 144.226 kb on + strand, within EX31_RS09715 at 144.312 kb on + strand, within EX31_RS09715 at 144.715 kb on + strand at 144.715 kb on + strand at 144.716 kb on - strand at 144.716 kb on - strand at 144.749 kb on + strand at 144.749 kb on + strand at 144.766 kb on - strand at 144.877 kb on + strand, within EX31_RS09720 at 144.878 kb on - strand, within EX31_RS09720 at 144.878 kb on - strand, within EX31_RS09720 at 144.893 kb on - strand, within EX31_RS09720 at 145.071 kb on + strand, within EX31_RS09720 at 145.071 kb on + strand, within EX31_RS09720 at 145.072 kb on - strand, within EX31_RS09720 at 145.073 kb on + strand, within EX31_RS09720 at 145.074 kb on - strand, within EX31_RS09720 at 145.115 kb on + strand, within EX31_RS09720 at 145.329 kb on + strand, within EX31_RS09720 at 145.370 kb on + strand, within EX31_RS09720 at 145.372 kb on + strand, within EX31_RS09720 at 145.372 kb on + strand, within EX31_RS09720 at 145.372 kb on + strand, within EX31_RS09720 at 145.372 kb on + strand, within EX31_RS09720 at 145.386 kb on + strand, within EX31_RS09720 at 145.500 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.513 kb on + strand, within EX31_RS09720 at 145.514 kb on - strand, within EX31_RS09720 at 145.514 kb on - strand, within EX31_RS09720 at 145.514 kb on - strand, within EX31_RS09720 at 145.514 kb on - strand, within EX31_RS09720 at 145.514 kb on - strand, within EX31_RS09720 at 145.515 kb on + strand, within EX31_RS09720 at 145.515 kb on + strand, within EX31_RS09720 at 145.515 kb on + strand, within EX31_RS09720 at 145.516 kb on - strand, within EX31_RS09720 at 145.525 kb on + strand, within EX31_RS09720 at 145.525 kb on + strand, within EX31_RS09720 at 145.537 kb on + strand, within EX31_RS09720 at 145.680 kb on + strand, within EX31_RS09720 at 145.680 kb on + strand, within EX31_RS09720 at 145.681 kb on - strand, within EX31_RS09720 at 145.681 kb on - strand, within EX31_RS09720
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 142,364 - EX31_RS09710 0.13 -0.2 142,364 - EX31_RS09710 0.13 -0.8 142,364 - EX31_RS09710 0.13 +0.9 142,364 - EX31_RS09710 0.13 -0.9 142,385 - EX31_RS09710 0.16 +1.7 142,425 + EX31_RS09710 0.20 +0.0 142,425 + EX31_RS09710 0.20 -0.3 142,426 - EX31_RS09710 0.20 -0.3 142,426 - EX31_RS09710 0.20 -0.9 142,429 + EX31_RS09710 0.20 -1.6 142,429 + EX31_RS09710 0.20 +0.7 143,038 + EX31_RS09710 0.86 -0.7 143,091 - +1.7 143,091 - +0.5 143,180 + -1.3 143,180 + +1.0 143,180 + -1.1 143,199 + -0.1 143,200 - +1.7 143,234 - +1.6 143,288 + -1.3 143,288 + -0.7 143,288 + +0.2 143,288 + +0.6 143,288 + +0.4 143,288 + -1.0 143,288 + -0.7 143,288 + +0.7 143,288 + +0.6 143,289 - +0.1 143,289 - -0.2 143,375 + -0.4 143,376 - +1.7 143,404 + +0.5 143,404 + +0.4 143,454 - +0.4 143,551 + EX31_RS09715 0.16 -0.7 143,552 - EX31_RS09715 0.16 -1.2 143,584 - EX31_RS09715 0.18 +1.4 143,722 + EX31_RS09715 0.28 +0.4 143,722 + EX31_RS09715 0.28 +1.7 143,725 + EX31_RS09715 0.28 +0.5 143,982 - EX31_RS09715 0.46 -0.3 144,072 + EX31_RS09715 0.53 +1.5 144,089 - EX31_RS09715 0.54 -1.3 144,215 + EX31_RS09715 0.63 -1.5 144,215 + EX31_RS09715 0.63 +0.3 144,216 - EX31_RS09715 0.63 -1.1 144,216 - EX31_RS09715 0.63 +1.1 144,226 + EX31_RS09715 0.64 -1.6 144,312 + EX31_RS09715 0.70 -0.4 144,715 + +0.7 144,715 + -1.3 144,716 - +0.9 144,716 - +0.1 144,749 + +1.1 144,749 + +0.7 144,766 - -1.7 144,877 + EX31_RS09720 0.11 +1.0 144,878 - EX31_RS09720 0.11 -0.4 144,878 - EX31_RS09720 0.11 +0.1 144,893 - EX31_RS09720 0.12 +0.7 145,071 + EX31_RS09720 0.26 +0.2 145,071 + EX31_RS09720 0.26 +2.3 145,072 - EX31_RS09720 0.26 +1.7 145,073 + EX31_RS09720 0.26 -0.0 145,074 - EX31_RS09720 0.26 +1.9 145,115 + EX31_RS09720 0.29 -0.3 145,329 + EX31_RS09720 0.46 -0.1 145,370 + EX31_RS09720 0.49 -1.9 145,372 + EX31_RS09720 0.49 -1.0 145,372 + EX31_RS09720 0.49 +1.1 145,372 + EX31_RS09720 0.49 +0.7 145,372 + EX31_RS09720 0.49 -0.5 145,386 + EX31_RS09720 0.50 +0.3 145,500 + EX31_RS09720 0.59 +0.6 145,513 + EX31_RS09720 0.60 +0.0 145,513 + EX31_RS09720 0.60 +2.7 145,513 + EX31_RS09720 0.60 +2.7 145,513 + EX31_RS09720 0.60 +0.4 145,513 + EX31_RS09720 0.60 -0.9 145,513 + EX31_RS09720 0.60 -0.1 145,513 + EX31_RS09720 0.60 -0.0 145,513 + EX31_RS09720 0.60 +0.0 145,513 + EX31_RS09720 0.60 +3.3 145,514 - EX31_RS09720 0.60 +1.7 145,514 - EX31_RS09720 0.60 +0.7 145,514 - EX31_RS09720 0.60 +1.0 145,514 - EX31_RS09720 0.60 +0.1 145,514 - EX31_RS09720 0.60 -2.1 145,515 + EX31_RS09720 0.60 +0.0 145,515 + EX31_RS09720 0.60 +0.5 145,515 + EX31_RS09720 0.60 +0.1 145,516 - EX31_RS09720 0.61 -0.2 145,525 + EX31_RS09720 0.61 +0.5 145,525 + EX31_RS09720 0.61 -1.6 145,537 + EX31_RS09720 0.62 -0.4 145,680 + EX31_RS09720 0.73 +0.1 145,680 + EX31_RS09720 0.73 +1.0 145,681 - EX31_RS09720 0.73 +0.5 145,681 - EX31_RS09720 0.73 +1.7
Or see this region's nucleotide sequence