Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09695 and EX31_RS09700 are separated by 308 nucleotides EX31_RS09700 and EX31_RS09705 are separated by 92 nucleotides EX31_RS09705 and EX31_RS09710 are separated by 3 nucleotides
EX31_RS09695: EX31_RS09695 - D-alanyl-D-alanine carboxypeptidase, at 139,570 to 140,781
_RS09695
EX31_RS09700: EX31_RS09700 - GlpM family protein, at 141,090 to 141,431
_RS09700
EX31_RS09705: EX31_RS09705 - HAD-IB family hydrolase, at 141,524 to 142,237
_RS09705
EX31_RS09710: EX31_RS09710 - LysR family transcriptional regulator, at 142,241 to 143,164
_RS09710
Position (kb)
141
142
143 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 140.558 kb on + strand, within EX31_RS09695 at 140.558 kb on + strand, within EX31_RS09695 at 140.559 kb on - strand, within EX31_RS09695 at 140.559 kb on - strand, within EX31_RS09695 at 140.559 kb on - strand, within EX31_RS09695 at 140.611 kb on - strand, within EX31_RS09695 at 140.640 kb on - strand, within EX31_RS09695 at 140.646 kb on + strand, within EX31_RS09695 at 140.647 kb on - strand, within EX31_RS09695 at 140.647 kb on - strand, within EX31_RS09695 at 140.651 kb on - strand, within EX31_RS09695 at 140.725 kb on + strand at 140.726 kb on - strand at 140.781 kb on + strand at 140.782 kb on - strand at 140.782 kb on - strand at 140.857 kb on - strand at 140.885 kb on - strand at 140.890 kb on + strand at 140.890 kb on + strand at 140.890 kb on + strand at 140.890 kb on + strand at 140.891 kb on - strand at 140.895 kb on - strand at 140.914 kb on + strand at 140.915 kb on - strand at 141.023 kb on + strand at 141.024 kb on - strand at 141.027 kb on + strand at 141.035 kb on + strand at 141.155 kb on + strand, within EX31_RS09700 at 141.168 kb on + strand, within EX31_RS09700 at 141.168 kb on + strand, within EX31_RS09700 at 141.169 kb on - strand, within EX31_RS09700 at 141.169 kb on - strand, within EX31_RS09700 at 141.171 kb on + strand, within EX31_RS09700 at 141.224 kb on + strand, within EX31_RS09700 at 141.224 kb on + strand, within EX31_RS09700 at 141.298 kb on - strand, within EX31_RS09700 at 141.298 kb on - strand, within EX31_RS09700 at 141.307 kb on - strand, within EX31_RS09700 at 141.351 kb on + strand, within EX31_RS09700 at 141.404 kb on - strand at 141.611 kb on + strand, within EX31_RS09705 at 141.667 kb on + strand, within EX31_RS09705 at 141.667 kb on + strand, within EX31_RS09705 at 141.667 kb on + strand, within EX31_RS09705 at 141.667 kb on + strand, within EX31_RS09705 at 141.668 kb on - strand, within EX31_RS09705 at 141.791 kb on - strand, within EX31_RS09705 at 141.853 kb on + strand, within EX31_RS09705 at 141.854 kb on - strand, within EX31_RS09705 at 141.878 kb on - strand, within EX31_RS09705 at 141.994 kb on + strand, within EX31_RS09705 at 141.995 kb on - strand, within EX31_RS09705 at 142.030 kb on - strand, within EX31_RS09705 at 142.064 kb on + strand, within EX31_RS09705 at 142.065 kb on - strand, within EX31_RS09705 at 142.065 kb on - strand, within EX31_RS09705 at 142.065 kb on - strand, within EX31_RS09705 at 142.065 kb on - strand, within EX31_RS09705 at 142.075 kb on - strand, within EX31_RS09705 at 142.090 kb on + strand, within EX31_RS09705 at 142.091 kb on - strand, within EX31_RS09705 at 142.141 kb on + strand, within EX31_RS09705 at 142.243 kb on - strand at 142.298 kb on - strand at 142.364 kb on - strand, within EX31_RS09710 at 142.364 kb on - strand, within EX31_RS09710 at 142.364 kb on - strand, within EX31_RS09710 at 142.364 kb on - strand, within EX31_RS09710 at 142.385 kb on - strand, within EX31_RS09710 at 142.425 kb on + strand, within EX31_RS09710 at 142.425 kb on + strand, within EX31_RS09710 at 142.426 kb on - strand, within EX31_RS09710 at 142.426 kb on - strand, within EX31_RS09710 at 142.429 kb on + strand, within EX31_RS09710 at 142.429 kb on + strand, within EX31_RS09710 at 143.038 kb on + strand, within EX31_RS09710 at 143.091 kb on - strand at 143.091 kb on - strand at 143.180 kb on + strand at 143.180 kb on + strand at 143.180 kb on + strand at 143.199 kb on + strand at 143.200 kb on - strand at 143.234 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 140,558 + EX31_RS09695 0.82 -0.1 140,558 + EX31_RS09695 0.82 +1.7 140,559 - EX31_RS09695 0.82 -1.1 140,559 - EX31_RS09695 0.82 -1.6 140,559 - EX31_RS09695 0.82 -0.3 140,611 - EX31_RS09695 0.86 +3.3 140,640 - EX31_RS09695 0.88 +0.5 140,646 + EX31_RS09695 0.89 -0.3 140,647 - EX31_RS09695 0.89 -0.9 140,647 - EX31_RS09695 0.89 +1.7 140,651 - EX31_RS09695 0.89 -2.6 140,725 + +1.5 140,726 - -0.2 140,781 + +1.0 140,782 - +1.0 140,782 - +0.8 140,857 - -1.4 140,885 - +0.8 140,890 + +0.1 140,890 + +0.5 140,890 + -0.2 140,890 + +1.7 140,891 - +0.5 140,895 - -1.0 140,914 + +1.7 140,915 - +0.6 141,023 + +0.0 141,024 - -1.7 141,027 + -0.6 141,035 + -0.3 141,155 + EX31_RS09700 0.19 -0.9 141,168 + EX31_RS09700 0.23 -1.5 141,168 + EX31_RS09700 0.23 -0.3 141,169 - EX31_RS09700 0.23 -1.3 141,169 - EX31_RS09700 0.23 +0.1 141,171 + EX31_RS09700 0.24 +1.3 141,224 + EX31_RS09700 0.39 +0.1 141,224 + EX31_RS09700 0.39 -1.4 141,298 - EX31_RS09700 0.61 -0.7 141,298 - EX31_RS09700 0.61 +0.8 141,307 - EX31_RS09700 0.63 -1.5 141,351 + EX31_RS09700 0.76 +0.1 141,404 - +0.7 141,611 + EX31_RS09705 0.12 +0.2 141,667 + EX31_RS09705 0.20 -0.4 141,667 + EX31_RS09705 0.20 +2.3 141,667 + EX31_RS09705 0.20 +0.7 141,667 + EX31_RS09705 0.20 -0.9 141,668 - EX31_RS09705 0.20 -1.0 141,791 - EX31_RS09705 0.37 -0.4 141,853 + EX31_RS09705 0.46 -2.0 141,854 - EX31_RS09705 0.46 +1.5 141,878 - EX31_RS09705 0.50 +0.1 141,994 + EX31_RS09705 0.66 +0.0 141,995 - EX31_RS09705 0.66 -1.5 142,030 - EX31_RS09705 0.71 -0.9 142,064 + EX31_RS09705 0.76 +0.8 142,065 - EX31_RS09705 0.76 -0.0 142,065 - EX31_RS09705 0.76 -1.1 142,065 - EX31_RS09705 0.76 +0.4 142,065 - EX31_RS09705 0.76 +0.4 142,075 - EX31_RS09705 0.77 +1.0 142,090 + EX31_RS09705 0.79 +1.6 142,091 - EX31_RS09705 0.79 -0.7 142,141 + EX31_RS09705 0.86 -0.4 142,243 - +3.0 142,298 - +0.0 142,364 - EX31_RS09710 0.13 -0.2 142,364 - EX31_RS09710 0.13 -0.8 142,364 - EX31_RS09710 0.13 +0.9 142,364 - EX31_RS09710 0.13 -0.9 142,385 - EX31_RS09710 0.16 +1.7 142,425 + EX31_RS09710 0.20 +0.0 142,425 + EX31_RS09710 0.20 -0.3 142,426 - EX31_RS09710 0.20 -0.3 142,426 - EX31_RS09710 0.20 -0.9 142,429 + EX31_RS09710 0.20 -1.6 142,429 + EX31_RS09710 0.20 +0.7 143,038 + EX31_RS09710 0.86 -0.7 143,091 - +1.7 143,091 - +0.5 143,180 + -1.3 143,180 + +1.0 143,180 + -1.1 143,199 + -0.1 143,200 - +1.7 143,234 - +1.6
Or see this region's nucleotide sequence