Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02775 and EX31_RS02780 are separated by 7 nucleotides EX31_RS02780 and EX31_RS02785 are separated by 103 nucleotides
EX31_RS02775: EX31_RS02775 - hypothetical protein, at 24,460 to 25,683
_RS02775
EX31_RS02780: EX31_RS02780 - oligosaccharide flippase family protein, at 25,691 to 26,908
_RS02780
EX31_RS02785: EX31_RS02785 - polysaccharide biosynthesis tyrosine autokinase, at 27,012 to 29,162
_RS02785
Position (kb) 25
26
27 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 24.694 kb on + strand, within EX31_RS02775 at 24.745 kb on + strand, within EX31_RS02775 at 24.747 kb on - strand, within EX31_RS02775 at 24.797 kb on + strand, within EX31_RS02775 at 24.797 kb on + strand, within EX31_RS02775 at 24.797 kb on + strand, within EX31_RS02775 at 24.798 kb on - strand, within EX31_RS02775 at 24.828 kb on + strand, within EX31_RS02775 at 25.421 kb on + strand, within EX31_RS02775 at 25.479 kb on - strand, within EX31_RS02775 at 25.656 kb on + strand at 25.728 kb on - strand at 25.901 kb on - strand, within EX31_RS02780 at 25.993 kb on - strand, within EX31_RS02780 at 26.264 kb on - strand, within EX31_RS02780 at 26.264 kb on - strand, within EX31_RS02780 at 26.377 kb on + strand, within EX31_RS02780 at 26.378 kb on - strand, within EX31_RS02780 at 26.557 kb on + strand, within EX31_RS02780 at 26.654 kb on + strand, within EX31_RS02780 at 26.654 kb on + strand, within EX31_RS02780 at 26.657 kb on - strand, within EX31_RS02780 at 26.716 kb on + strand, within EX31_RS02780 at 26.811 kb on - strand at 26.878 kb on - strand at 26.878 kb on - strand at 26.878 kb on - strand at 26.881 kb on + strand at 26.884 kb on + strand at 27.081 kb on - strand at 27.120 kb on - strand at 27.133 kb on - strand at 27.198 kb on - strand at 27.198 kb on - strand at 27.205 kb on + strand at 27.206 kb on - strand at 27.209 kb on + strand at 27.209 kb on + strand at 27.213 kb on + strand at 27.259 kb on - strand, within EX31_RS02785 at 27.359 kb on + strand, within EX31_RS02785 at 27.380 kb on + strand, within EX31_RS02785 at 27.384 kb on - strand, within EX31_RS02785 at 27.446 kb on + strand, within EX31_RS02785 at 27.447 kb on - strand, within EX31_RS02785 at 27.447 kb on - strand, within EX31_RS02785 at 27.495 kb on - strand, within EX31_RS02785 at 27.495 kb on - strand, within EX31_RS02785 at 27.495 kb on - strand, within EX31_RS02785 at 27.496 kb on + strand, within EX31_RS02785 at 27.496 kb on + strand, within EX31_RS02785 at 27.496 kb on + strand, within EX31_RS02785 at 27.496 kb on + strand, within EX31_RS02785 at 27.497 kb on - strand, within EX31_RS02785 at 27.497 kb on - strand, within EX31_RS02785 at 27.497 kb on - strand, within EX31_RS02785 at 27.497 kb on - strand, within EX31_RS02785 at 27.497 kb on - strand, within EX31_RS02785 at 27.497 kb on - strand, within EX31_RS02785 at 27.497 kb on - strand, within EX31_RS02785 at 27.497 kb on - strand, within EX31_RS02785 at 27.498 kb on + strand, within EX31_RS02785 at 27.498 kb on + strand, within EX31_RS02785 at 27.498 kb on + strand, within EX31_RS02785 at 27.498 kb on + strand, within EX31_RS02785 at 27.498 kb on + strand, within EX31_RS02785 at 27.499 kb on - strand, within EX31_RS02785 at 27.499 kb on - strand, within EX31_RS02785 at 27.499 kb on - strand, within EX31_RS02785 at 27.499 kb on - strand, within EX31_RS02785 at 27.499 kb on - strand, within EX31_RS02785 at 27.499 kb on - strand, within EX31_RS02785 at 27.499 kb on - strand, within EX31_RS02785 at 27.562 kb on - strand, within EX31_RS02785 at 27.654 kb on + strand, within EX31_RS02785 at 27.655 kb on - strand, within EX31_RS02785 at 27.685 kb on - strand, within EX31_RS02785 at 27.693 kb on - strand, within EX31_RS02785 at 27.693 kb on - strand, within EX31_RS02785 at 27.705 kb on - strand, within EX31_RS02785 at 27.713 kb on + strand, within EX31_RS02785 at 27.714 kb on - strand, within EX31_RS02785 at 27.714 kb on - strand, within EX31_RS02785 at 27.725 kb on - strand, within EX31_RS02785 at 27.725 kb on - strand, within EX31_RS02785 at 27.725 kb on - strand, within EX31_RS02785 at 27.770 kb on + strand, within EX31_RS02785 at 27.771 kb on - strand, within EX31_RS02785 at 27.771 kb on - strand, within EX31_RS02785 at 27.771 kb on - strand, within EX31_RS02785 at 27.790 kb on - strand, within EX31_RS02785 at 27.790 kb on - strand, within EX31_RS02785 at 27.835 kb on - strand, within EX31_RS02785 at 27.837 kb on - strand, within EX31_RS02785
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 24,694 + EX31_RS02775 0.19 +2.3 24,745 + EX31_RS02775 0.23 +0.5 24,747 - EX31_RS02775 0.23 -1.6 24,797 + EX31_RS02775 0.28 +0.7 24,797 + EX31_RS02775 0.28 +0.4 24,797 + EX31_RS02775 0.28 -0.6 24,798 - EX31_RS02775 0.28 -0.1 24,828 + EX31_RS02775 0.30 +0.2 25,421 + EX31_RS02775 0.79 +0.3 25,479 - EX31_RS02775 0.83 +0.2 25,656 + -0.2 25,728 - +0.0 25,901 - EX31_RS02780 0.17 +1.3 25,993 - EX31_RS02780 0.25 +0.7 26,264 - EX31_RS02780 0.47 +0.1 26,264 - EX31_RS02780 0.47 -0.2 26,377 + EX31_RS02780 0.56 +0.5 26,378 - EX31_RS02780 0.56 -1.9 26,557 + EX31_RS02780 0.71 +0.9 26,654 + EX31_RS02780 0.79 -0.2 26,654 + EX31_RS02780 0.79 +0.0 26,657 - EX31_RS02780 0.79 +0.0 26,716 + EX31_RS02780 0.84 +1.6 26,811 - +0.0 26,878 - -0.1 26,878 - -0.4 26,878 - +0.1 26,881 + +0.3 26,884 + +1.3 27,081 - +0.8 27,120 - -0.2 27,133 - -0.3 27,198 - +1.5 27,198 - -0.3 27,205 + +1.2 27,206 - +0.2 27,209 + +0.6 27,209 + +0.4 27,213 + -0.9 27,259 - EX31_RS02785 0.11 +0.4 27,359 + EX31_RS02785 0.16 +0.5 27,380 + EX31_RS02785 0.17 +0.2 27,384 - EX31_RS02785 0.17 +0.9 27,446 + EX31_RS02785 0.20 +1.1 27,447 - EX31_RS02785 0.20 +0.7 27,447 - EX31_RS02785 0.20 -1.2 27,495 - EX31_RS02785 0.22 -0.5 27,495 - EX31_RS02785 0.22 +3.0 27,495 - EX31_RS02785 0.22 +0.1 27,496 + EX31_RS02785 0.23 -0.1 27,496 + EX31_RS02785 0.23 +0.7 27,496 + EX31_RS02785 0.23 +1.3 27,496 + EX31_RS02785 0.23 +0.4 27,497 - EX31_RS02785 0.23 +1.3 27,497 - EX31_RS02785 0.23 +0.4 27,497 - EX31_RS02785 0.23 +1.7 27,497 - EX31_RS02785 0.23 -0.5 27,497 - EX31_RS02785 0.23 +1.0 27,497 - EX31_RS02785 0.23 -1.8 27,497 - EX31_RS02785 0.23 -0.5 27,497 - EX31_RS02785 0.23 -0.6 27,498 + EX31_RS02785 0.23 +0.3 27,498 + EX31_RS02785 0.23 +0.8 27,498 + EX31_RS02785 0.23 +0.4 27,498 + EX31_RS02785 0.23 -0.3 27,498 + EX31_RS02785 0.23 +0.4 27,499 - EX31_RS02785 0.23 +0.2 27,499 - EX31_RS02785 0.23 -0.5 27,499 - EX31_RS02785 0.23 +0.5 27,499 - EX31_RS02785 0.23 +0.8 27,499 - EX31_RS02785 0.23 -2.2 27,499 - EX31_RS02785 0.23 -0.4 27,499 - EX31_RS02785 0.23 +0.8 27,562 - EX31_RS02785 0.26 +0.8 27,654 + EX31_RS02785 0.30 +0.7 27,655 - EX31_RS02785 0.30 +0.2 27,685 - EX31_RS02785 0.31 -0.1 27,693 - EX31_RS02785 0.32 +0.5 27,693 - EX31_RS02785 0.32 -0.0 27,705 - EX31_RS02785 0.32 +0.1 27,713 + EX31_RS02785 0.33 +2.0 27,714 - EX31_RS02785 0.33 -0.8 27,714 - EX31_RS02785 0.33 +0.6 27,725 - EX31_RS02785 0.33 -0.1 27,725 - EX31_RS02785 0.33 +0.3 27,725 - EX31_RS02785 0.33 +0.7 27,770 + EX31_RS02785 0.35 +0.6 27,771 - EX31_RS02785 0.35 -1.0 27,771 - EX31_RS02785 0.35 +1.3 27,771 - EX31_RS02785 0.35 +1.2 27,790 - EX31_RS02785 0.36 +4.0 27,790 - EX31_RS02785 0.36 -0.5 27,835 - EX31_RS02785 0.38 +1.0 27,837 - EX31_RS02785 0.38 +0.3
Or see this region's nucleotide sequence