Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23695 and EX31_RS23700 are separated by 3 nucleotides EX31_RS23700 and EX31_RS23705 overlap by 17 nucleotides EX31_RS23705 and EX31_RS23710 are separated by 147 nucleotides
EX31_RS23695: EX31_RS23695 - biotin synthase BioB, at 503,897 to 504,934
_RS23695
EX31_RS23700: EX31_RS23700 - 8-amino-7-oxononanoate synthase, at 504,938 to 506,089
_RS23700
EX31_RS23705: EX31_RS23705 - malonyl-ACP O-methyltransferase BioC, at 506,073 to 506,864
_RS23705
EX31_RS23710: EX31_RS23710 - ATP-dependent dethiobiotin synthetase BioD, at 507,012 to 507,692
_RS23710
Position (kb) 504
505
506
507 Strain fitness (log2 ratio) -2
-1
0
1
2 at 503.947 kb on + strand at 504.067 kb on + strand, within EX31_RS23695 at 504.067 kb on + strand, within EX31_RS23695 at 504.067 kb on + strand, within EX31_RS23695 at 504.068 kb on - strand, within EX31_RS23695 at 504.068 kb on - strand, within EX31_RS23695 at 504.072 kb on - strand, within EX31_RS23695 at 504.305 kb on - strand, within EX31_RS23695 at 504.305 kb on - strand, within EX31_RS23695 at 504.305 kb on - strand, within EX31_RS23695 at 504.305 kb on - strand, within EX31_RS23695 at 504.352 kb on + strand, within EX31_RS23695 at 504.352 kb on + strand, within EX31_RS23695 at 504.361 kb on + strand, within EX31_RS23695 at 504.361 kb on + strand, within EX31_RS23695 at 504.361 kb on + strand, within EX31_RS23695 at 504.391 kb on - strand, within EX31_RS23695 at 504.404 kb on + strand, within EX31_RS23695 at 504.422 kb on - strand, within EX31_RS23695 at 504.422 kb on - strand, within EX31_RS23695 at 504.451 kb on + strand, within EX31_RS23695 at 504.452 kb on - strand, within EX31_RS23695 at 504.480 kb on + strand, within EX31_RS23695 at 504.480 kb on + strand, within EX31_RS23695 at 504.480 kb on + strand, within EX31_RS23695 at 504.583 kb on + strand, within EX31_RS23695 at 504.668 kb on + strand, within EX31_RS23695 at 504.668 kb on + strand, within EX31_RS23695 at 504.668 kb on + strand, within EX31_RS23695 at 504.668 kb on + strand, within EX31_RS23695 at 504.668 kb on + strand, within EX31_RS23695 at 504.668 kb on + strand, within EX31_RS23695 at 504.668 kb on + strand, within EX31_RS23695 at 504.668 kb on + strand, within EX31_RS23695 at 504.668 kb on + strand, within EX31_RS23695 at 504.668 kb on + strand, within EX31_RS23695 at 504.668 kb on + strand, within EX31_RS23695 at 504.669 kb on - strand, within EX31_RS23695 at 504.669 kb on - strand, within EX31_RS23695 at 504.669 kb on - strand, within EX31_RS23695 at 504.669 kb on - strand, within EX31_RS23695 at 504.669 kb on - strand, within EX31_RS23695 at 504.728 kb on - strand, within EX31_RS23695 at 504.753 kb on - strand, within EX31_RS23695 at 504.793 kb on + strand, within EX31_RS23695 at 504.794 kb on - strand, within EX31_RS23695 at 504.794 kb on - strand, within EX31_RS23695 at 504.917 kb on + strand at 504.917 kb on + strand at 504.917 kb on + strand at 504.917 kb on + strand at 504.917 kb on + strand at 504.917 kb on + strand at 504.917 kb on + strand at 504.918 kb on - strand at 504.918 kb on - strand at 504.930 kb on + strand at 505.002 kb on + strand at 505.082 kb on + strand, within EX31_RS23700 at 505.142 kb on + strand, within EX31_RS23700 at 505.142 kb on + strand, within EX31_RS23700 at 505.142 kb on + strand, within EX31_RS23700 at 505.142 kb on + strand, within EX31_RS23700 at 505.142 kb on + strand, within EX31_RS23700 at 505.142 kb on + strand, within EX31_RS23700 at 505.142 kb on + strand, within EX31_RS23700 at 505.143 kb on - strand, within EX31_RS23700 at 505.143 kb on - strand, within EX31_RS23700 at 505.253 kb on - strand, within EX31_RS23700 at 505.468 kb on - strand, within EX31_RS23700 at 505.468 kb on - strand, within EX31_RS23700 at 505.468 kb on - strand, within EX31_RS23700 at 505.468 kb on - strand, within EX31_RS23700 at 505.510 kb on + strand, within EX31_RS23700 at 505.510 kb on + strand, within EX31_RS23700 at 505.531 kb on + strand, within EX31_RS23700 at 505.728 kb on - strand, within EX31_RS23700 at 505.728 kb on - strand, within EX31_RS23700 at 505.728 kb on - strand, within EX31_RS23700 at 505.728 kb on - strand, within EX31_RS23700 at 505.728 kb on - strand, within EX31_RS23700 at 505.825 kb on + strand, within EX31_RS23700 at 506.014 kb on + strand at 506.014 kb on + strand at 506.014 kb on + strand at 506.015 kb on - strand at 506.015 kb on - strand at 506.015 kb on - strand at 506.015 kb on - strand at 506.095 kb on - strand at 506.205 kb on + strand, within EX31_RS23705 at 506.206 kb on - strand, within EX31_RS23705 at 506.323 kb on + strand, within EX31_RS23705 at 506.358 kb on + strand, within EX31_RS23705 at 506.358 kb on + strand, within EX31_RS23705 at 506.358 kb on + strand, within EX31_RS23705 at 506.358 kb on + strand, within EX31_RS23705 at 506.358 kb on + strand, within EX31_RS23705 at 506.359 kb on - strand, within EX31_RS23705 at 506.359 kb on - strand, within EX31_RS23705 at 506.359 kb on - strand, within EX31_RS23705 at 506.359 kb on - strand, within EX31_RS23705 at 506.781 kb on + strand, within EX31_RS23705 at 506.781 kb on + strand, within EX31_RS23705 at 506.781 kb on + strand, within EX31_RS23705 at 506.781 kb on + strand, within EX31_RS23705 at 506.782 kb on - strand, within EX31_RS23705 at 506.826 kb on + strand at 506.827 kb on - strand at 506.863 kb on - strand at 506.867 kb on - strand at 506.867 kb on - strand at 506.867 kb on - strand at 506.867 kb on - strand at 506.867 kb on - strand at 506.867 kb on - strand at 506.938 kb on - strand at 506.938 kb on - strand at 506.941 kb on - strand at 506.998 kb on + strand at 507.006 kb on + strand at 507.007 kb on - strand at 507.024 kb on + strand at 507.024 kb on + strand at 507.024 kb on + strand at 507.024 kb on + strand at 507.025 kb on - strand at 507.025 kb on - strand at 507.030 kb on - strand at 507.030 kb on - strand at 507.056 kb on + strand at 507.057 kb on - strand at 507.057 kb on - strand at 507.057 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 503,947 + -0.8 504,067 + EX31_RS23695 0.16 -0.5 504,067 + EX31_RS23695 0.16 -0.4 504,067 + EX31_RS23695 0.16 -1.0 504,068 - EX31_RS23695 0.16 +0.2 504,068 - EX31_RS23695 0.16 +1.0 504,072 - EX31_RS23695 0.17 +0.5 504,305 - EX31_RS23695 0.39 -0.9 504,305 - EX31_RS23695 0.39 +0.8 504,305 - EX31_RS23695 0.39 +0.0 504,305 - EX31_RS23695 0.39 +0.2 504,352 + EX31_RS23695 0.44 -0.1 504,352 + EX31_RS23695 0.44 +0.8 504,361 + EX31_RS23695 0.45 +0.4 504,361 + EX31_RS23695 0.45 -1.1 504,361 + EX31_RS23695 0.45 -1.6 504,391 - EX31_RS23695 0.48 -0.1 504,404 + EX31_RS23695 0.49 +0.2 504,422 - EX31_RS23695 0.51 -0.2 504,422 - EX31_RS23695 0.51 -0.4 504,451 + EX31_RS23695 0.53 -1.4 504,452 - EX31_RS23695 0.53 -0.5 504,480 + EX31_RS23695 0.56 -0.2 504,480 + EX31_RS23695 0.56 +1.5 504,480 + EX31_RS23695 0.56 +0.2 504,583 + EX31_RS23695 0.66 -0.7 504,668 + EX31_RS23695 0.74 +0.3 504,668 + EX31_RS23695 0.74 +0.7 504,668 + EX31_RS23695 0.74 +0.1 504,668 + EX31_RS23695 0.74 +0.4 504,668 + EX31_RS23695 0.74 -1.0 504,668 + EX31_RS23695 0.74 +2.2 504,668 + EX31_RS23695 0.74 +0.2 504,668 + EX31_RS23695 0.74 +0.8 504,668 + EX31_RS23695 0.74 -2.3 504,668 + EX31_RS23695 0.74 -0.5 504,668 + EX31_RS23695 0.74 -1.4 504,669 - EX31_RS23695 0.74 +0.6 504,669 - EX31_RS23695 0.74 -0.1 504,669 - EX31_RS23695 0.74 -0.3 504,669 - EX31_RS23695 0.74 -0.7 504,669 - EX31_RS23695 0.74 -0.4 504,728 - EX31_RS23695 0.80 +0.8 504,753 - EX31_RS23695 0.82 +0.7 504,793 + EX31_RS23695 0.86 -0.7 504,794 - EX31_RS23695 0.86 -0.7 504,794 - EX31_RS23695 0.86 +0.7 504,917 + -0.6 504,917 + +0.2 504,917 + +0.8 504,917 + -0.6 504,917 + -0.1 504,917 + +0.5 504,917 + +0.6 504,918 - +1.4 504,918 - +0.9 504,930 + -1.5 505,002 + -0.7 505,082 + EX31_RS23700 0.12 -1.2 505,142 + EX31_RS23700 0.18 +0.6 505,142 + EX31_RS23700 0.18 +1.1 505,142 + EX31_RS23700 0.18 +1.3 505,142 + EX31_RS23700 0.18 +1.3 505,142 + EX31_RS23700 0.18 +0.6 505,142 + EX31_RS23700 0.18 +0.5 505,142 + EX31_RS23700 0.18 +1.0 505,143 - EX31_RS23700 0.18 +0.5 505,143 - EX31_RS23700 0.18 +0.5 505,253 - EX31_RS23700 0.27 +0.8 505,468 - EX31_RS23700 0.46 +0.8 505,468 - EX31_RS23700 0.46 +2.1 505,468 - EX31_RS23700 0.46 +1.3 505,468 - EX31_RS23700 0.46 -0.1 505,510 + EX31_RS23700 0.50 -0.4 505,510 + EX31_RS23700 0.50 +0.2 505,531 + EX31_RS23700 0.51 -1.3 505,728 - EX31_RS23700 0.69 +1.0 505,728 - EX31_RS23700 0.69 +1.1 505,728 - EX31_RS23700 0.69 -0.4 505,728 - EX31_RS23700 0.69 +0.7 505,728 - EX31_RS23700 0.69 +0.8 505,825 + EX31_RS23700 0.77 +0.3 506,014 + +0.0 506,014 + +1.2 506,014 + +0.5 506,015 - +1.0 506,015 - +0.0 506,015 - -0.7 506,015 - +0.5 506,095 - +0.4 506,205 + EX31_RS23705 0.17 -1.4 506,206 - EX31_RS23705 0.17 +2.1 506,323 + EX31_RS23705 0.32 -0.5 506,358 + EX31_RS23705 0.36 +0.0 506,358 + EX31_RS23705 0.36 -1.7 506,358 + EX31_RS23705 0.36 +0.1 506,358 + EX31_RS23705 0.36 -0.1 506,358 + EX31_RS23705 0.36 -0.3 506,359 - EX31_RS23705 0.36 +0.8 506,359 - EX31_RS23705 0.36 +0.1 506,359 - EX31_RS23705 0.36 -0.1 506,359 - EX31_RS23705 0.36 +0.8 506,781 + EX31_RS23705 0.89 +0.7 506,781 + EX31_RS23705 0.89 -0.5 506,781 + EX31_RS23705 0.89 +0.1 506,781 + EX31_RS23705 0.89 -1.0 506,782 - EX31_RS23705 0.90 -0.3 506,826 + -0.5 506,827 - +0.7 506,863 - +0.2 506,867 - +0.8 506,867 - -0.2 506,867 - -1.8 506,867 - -0.5 506,867 - -1.9 506,867 - +1.4 506,938 - -0.5 506,938 - -0.3 506,941 - +0.5 506,998 + +0.6 507,006 + +0.8 507,007 - +0.5 507,024 + +0.5 507,024 + -0.6 507,024 + +0.1 507,024 + +0.5 507,025 - -0.1 507,025 - -1.1 507,030 - -1.5 507,030 - -1.3 507,056 + -1.3 507,057 - +1.2 507,057 - -1.1 507,057 - -0.2
Or see this region's nucleotide sequence