Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23480 and EX31_RS23485 are separated by 11 nucleotides EX31_RS23485 and EX31_RS23490 are separated by 113 nucleotides EX31_RS23490 and EX31_RS23495 are separated by 129 nucleotides
EX31_RS23480: EX31_RS23480 - Tol-Pal system protein TolQ, at 465,007 to 465,696
_RS23480
EX31_RS23485: EX31_RS23485 - colicin uptake protein TolR, at 465,708 to 466,139
_RS23485
EX31_RS23490: EX31_RS23490 - cell envelope integrity protein TolA, at 466,253 to 467,488
_RS23490
EX31_RS23495: EX31_RS23495 - Tol-Pal system protein TolB, at 467,618 to 468,910
_RS23495
Position (kb) 466
467
468 Strain fitness (log2 ratio) -2
-1
0
1
2 at 465.345 kb on + strand, within EX31_RS23480 at 465.354 kb on + strand, within EX31_RS23480 at 465.421 kb on + strand, within EX31_RS23480 at 465.421 kb on + strand, within EX31_RS23480 at 465.421 kb on + strand, within EX31_RS23480 at 465.421 kb on + strand, within EX31_RS23480 at 465.422 kb on - strand, within EX31_RS23480 at 465.422 kb on - strand, within EX31_RS23480 at 465.423 kb on + strand, within EX31_RS23480 at 465.423 kb on + strand, within EX31_RS23480 at 465.423 kb on + strand, within EX31_RS23480 at 465.580 kb on + strand, within EX31_RS23480 at 465.690 kb on + strand at 465.694 kb on + strand at 465.694 kb on + strand at 465.707 kb on + strand at 465.781 kb on + strand, within EX31_RS23485 at 465.869 kb on + strand, within EX31_RS23485 at 465.930 kb on + strand, within EX31_RS23485 at 465.957 kb on - strand, within EX31_RS23485 at 466.027 kb on + strand, within EX31_RS23485 at 466.059 kb on + strand, within EX31_RS23485 at 466.070 kb on + strand, within EX31_RS23485 at 466.073 kb on + strand, within EX31_RS23485 at 466.133 kb on + strand at 466.133 kb on + strand at 466.133 kb on + strand at 466.133 kb on + strand at 466.134 kb on - strand at 466.143 kb on + strand at 466.143 kb on + strand at 466.143 kb on + strand at 466.147 kb on + strand at 466.147 kb on + strand at 466.147 kb on + strand at 466.147 kb on + strand at 466.147 kb on + strand at 466.148 kb on - strand at 466.154 kb on + strand at 466.155 kb on - strand at 466.155 kb on - strand at 466.161 kb on + strand at 466.161 kb on + strand at 466.161 kb on + strand at 466.161 kb on + strand at 466.161 kb on + strand at 466.161 kb on + strand at 466.162 kb on - strand at 466.162 kb on - strand at 466.190 kb on + strand at 466.190 kb on + strand at 466.196 kb on + strand at 466.197 kb on - strand at 466.226 kb on + strand at 466.229 kb on + strand at 466.230 kb on - strand at 466.249 kb on + strand at 466.324 kb on + strand at 466.333 kb on + strand at 466.369 kb on + strand at 466.445 kb on + strand, within EX31_RS23490 at 466.771 kb on + strand, within EX31_RS23490 at 467.237 kb on + strand, within EX31_RS23490 at 467.237 kb on + strand, within EX31_RS23490 at 467.237 kb on + strand, within EX31_RS23490 at 467.237 kb on + strand, within EX31_RS23490 at 467.276 kb on + strand, within EX31_RS23490 at 467.291 kb on + strand, within EX31_RS23490 at 467.291 kb on + strand, within EX31_RS23490 at 467.293 kb on + strand, within EX31_RS23490 at 467.293 kb on + strand, within EX31_RS23490 at 467.537 kb on + strand at 467.537 kb on + strand at 467.538 kb on - strand at 467.538 kb on - strand at 467.543 kb on + strand at 467.595 kb on + strand at 467.595 kb on + strand at 467.595 kb on + strand at 467.605 kb on + strand at 467.605 kb on + strand at 467.614 kb on + strand at 467.614 kb on + strand at 467.614 kb on + strand at 467.614 kb on + strand at 467.614 kb on + strand at 467.614 kb on + strand at 467.713 kb on + strand at 467.713 kb on + strand at 467.713 kb on + strand at 467.802 kb on + strand, within EX31_RS23495 at 467.802 kb on + strand, within EX31_RS23495 at 467.802 kb on + strand, within EX31_RS23495 at 467.802 kb on + strand, within EX31_RS23495 at 467.806 kb on + strand, within EX31_RS23495 at 467.806 kb on + strand, within EX31_RS23495 at 467.827 kb on + strand, within EX31_RS23495 at 467.827 kb on + strand, within EX31_RS23495 at 467.842 kb on + strand, within EX31_RS23495 at 467.842 kb on + strand, within EX31_RS23495 at 467.951 kb on + strand, within EX31_RS23495 at 467.951 kb on + strand, within EX31_RS23495 at 467.951 kb on + strand, within EX31_RS23495 at 467.951 kb on + strand, within EX31_RS23495 at 467.977 kb on + strand, within EX31_RS23495 at 467.977 kb on + strand, within EX31_RS23495 at 468.122 kb on + strand, within EX31_RS23495 at 468.122 kb on + strand, within EX31_RS23495 at 468.122 kb on + strand, within EX31_RS23495 at 468.124 kb on + strand, within EX31_RS23495 at 468.124 kb on + strand, within EX31_RS23495 at 468.185 kb on + strand, within EX31_RS23495 at 468.185 kb on + strand, within EX31_RS23495 at 468.263 kb on + strand, within EX31_RS23495 at 468.263 kb on + strand, within EX31_RS23495 at 468.428 kb on + strand, within EX31_RS23495 at 468.428 kb on + strand, within EX31_RS23495
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 465,345 + EX31_RS23480 0.49 -1.9 465,354 + EX31_RS23480 0.50 -0.4 465,421 + EX31_RS23480 0.60 -0.8 465,421 + EX31_RS23480 0.60 -1.2 465,421 + EX31_RS23480 0.60 -1.1 465,421 + EX31_RS23480 0.60 +0.8 465,422 - EX31_RS23480 0.60 +1.8 465,422 - EX31_RS23480 0.60 +0.5 465,423 + EX31_RS23480 0.60 -1.1 465,423 + EX31_RS23480 0.60 -1.3 465,423 + EX31_RS23480 0.60 -0.4 465,580 + EX31_RS23480 0.83 -1.1 465,690 + -0.5 465,694 + -0.2 465,694 + -0.1 465,707 + -0.8 465,781 + EX31_RS23485 0.17 -1.5 465,869 + EX31_RS23485 0.37 -0.2 465,930 + EX31_RS23485 0.51 -1.0 465,957 - EX31_RS23485 0.58 +1.2 466,027 + EX31_RS23485 0.74 -1.5 466,059 + EX31_RS23485 0.81 +0.2 466,070 + EX31_RS23485 0.84 -2.0 466,073 + EX31_RS23485 0.84 +0.8 466,133 + +1.7 466,133 + +0.5 466,133 + -0.9 466,133 + -1.2 466,134 - -2.2 466,143 + -0.8 466,143 + -0.6 466,143 + -1.9 466,147 + +0.2 466,147 + +1.0 466,147 + +0.4 466,147 + +0.2 466,147 + -0.5 466,148 - -1.0 466,154 + +0.1 466,155 - -0.2 466,155 - -1.5 466,161 + -0.3 466,161 + -0.2 466,161 + -0.7 466,161 + +0.1 466,161 + -0.6 466,161 + +0.8 466,162 - -0.2 466,162 - -1.1 466,190 + +0.9 466,190 + +0.5 466,196 + +0.4 466,197 - +0.4 466,226 + -0.3 466,229 + +0.6 466,230 - -0.6 466,249 + +0.5 466,324 + -1.3 466,333 + -1.0 466,369 + -2.1 466,445 + EX31_RS23490 0.16 +0.2 466,771 + EX31_RS23490 0.42 +0.5 467,237 + EX31_RS23490 0.80 -0.5 467,237 + EX31_RS23490 0.80 -1.2 467,237 + EX31_RS23490 0.80 -1.8 467,237 + EX31_RS23490 0.80 -1.8 467,276 + EX31_RS23490 0.83 -1.2 467,291 + EX31_RS23490 0.84 -1.3 467,291 + EX31_RS23490 0.84 -0.3 467,293 + EX31_RS23490 0.84 -1.8 467,293 + EX31_RS23490 0.84 -2.0 467,537 + -0.2 467,537 + +0.9 467,538 - -1.8 467,538 - -1.6 467,543 + -0.2 467,595 + +2.4 467,595 + -1.0 467,595 + +0.1 467,605 + -0.1 467,605 + -0.0 467,614 + -0.1 467,614 + -2.0 467,614 + +0.6 467,614 + -0.2 467,614 + -1.3 467,614 + -0.8 467,713 + -1.5 467,713 + -2.1 467,713 + -2.6 467,802 + EX31_RS23495 0.14 -1.1 467,802 + EX31_RS23495 0.14 -1.5 467,802 + EX31_RS23495 0.14 -1.0 467,802 + EX31_RS23495 0.14 -0.6 467,806 + EX31_RS23495 0.15 -1.3 467,806 + EX31_RS23495 0.15 -0.5 467,827 + EX31_RS23495 0.16 -1.1 467,827 + EX31_RS23495 0.16 +0.8 467,842 + EX31_RS23495 0.17 -0.8 467,842 + EX31_RS23495 0.17 -1.2 467,951 + EX31_RS23495 0.26 +0.5 467,951 + EX31_RS23495 0.26 -0.2 467,951 + EX31_RS23495 0.26 -0.7 467,951 + EX31_RS23495 0.26 -0.5 467,977 + EX31_RS23495 0.28 +0.3 467,977 + EX31_RS23495 0.28 -1.2 468,122 + EX31_RS23495 0.39 -2.0 468,122 + EX31_RS23495 0.39 -0.5 468,122 + EX31_RS23495 0.39 -1.2 468,124 + EX31_RS23495 0.39 -2.1 468,124 + EX31_RS23495 0.39 -2.2 468,185 + EX31_RS23495 0.44 -2.0 468,185 + EX31_RS23495 0.44 +0.2 468,263 + EX31_RS23495 0.50 -0.9 468,263 + EX31_RS23495 0.50 -2.6 468,428 + EX31_RS23495 0.63 -1.0 468,428 + EX31_RS23495 0.63 +0.8
Or see this region's nucleotide sequence