Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20695 and EX31_RS20700 are separated by 114 nucleotides EX31_RS20700 and EX31_RS20705 are separated by 27 nucleotides EX31_RS20705 and EX31_RS20710 are separated by 114 nucleotides
EX31_RS20695: EX31_RS20695 - Pr2TM family membrane protein, at 782,083 to 782,562
_RS20695
EX31_RS20700: EX31_RS20700 - GNAT family N-acetyltransferase, at 782,677 to 783,168
_RS20700
EX31_RS20705: EX31_RS20705 - LysR family transcriptional regulator, at 783,196 to 784,104
_RS20705
EX31_RS20710: EX31_RS20710 - serine hydrolase, at 784,219 to 785,370
_RS20710
Position (kb) 783
784
785 Strain fitness (log2 ratio) -2
-1
0
1
2 at 782.231 kb on + strand, within EX31_RS20695 at 782.231 kb on + strand, within EX31_RS20695 at 782.231 kb on + strand, within EX31_RS20695 at 782.231 kb on + strand, within EX31_RS20695 at 782.309 kb on - strand, within EX31_RS20695 at 782.315 kb on + strand, within EX31_RS20695 at 782.315 kb on + strand, within EX31_RS20695 at 782.329 kb on + strand, within EX31_RS20695 at 782.404 kb on - strand, within EX31_RS20695 at 782.437 kb on + strand, within EX31_RS20695 at 782.437 kb on + strand, within EX31_RS20695 at 782.437 kb on + strand, within EX31_RS20695 at 782.438 kb on - strand, within EX31_RS20695 at 782.438 kb on - strand, within EX31_RS20695 at 782.438 kb on - strand, within EX31_RS20695 at 782.576 kb on - strand at 782.701 kb on - strand at 782.758 kb on - strand, within EX31_RS20700 at 782.775 kb on - strand, within EX31_RS20700 at 782.867 kb on - strand, within EX31_RS20700 at 782.885 kb on + strand, within EX31_RS20700 at 783.152 kb on - strand at 783.152 kb on - strand at 783.152 kb on - strand at 783.181 kb on + strand at 783.181 kb on + strand at 783.182 kb on - strand at 783.182 kb on - strand at 783.182 kb on - strand at 783.182 kb on - strand at 783.182 kb on - strand at 783.185 kb on + strand at 783.197 kb on + strand at 783.198 kb on - strand at 783.198 kb on - strand at 783.198 kb on - strand at 783.198 kb on - strand at 783.208 kb on + strand at 783.208 kb on + strand at 783.208 kb on + strand at 783.209 kb on - strand at 783.209 kb on - strand at 783.209 kb on - strand at 783.209 kb on - strand at 783.285 kb on + strand at 783.285 kb on + strand at 783.285 kb on + strand at 783.285 kb on + strand at 783.285 kb on + strand at 783.285 kb on + strand at 783.286 kb on - strand at 783.286 kb on - strand at 783.286 kb on - strand at 783.286 kb on - strand at 783.286 kb on - strand at 783.286 kb on - strand at 783.286 kb on - strand at 783.286 kb on - strand at 783.286 kb on - strand at 783.287 kb on + strand, within EX31_RS20705 at 783.288 kb on - strand, within EX31_RS20705 at 783.304 kb on + strand, within EX31_RS20705 at 783.305 kb on - strand, within EX31_RS20705 at 783.305 kb on - strand, within EX31_RS20705 at 783.305 kb on - strand, within EX31_RS20705 at 783.311 kb on + strand, within EX31_RS20705 at 783.312 kb on - strand, within EX31_RS20705 at 783.335 kb on + strand, within EX31_RS20705 at 783.335 kb on + strand, within EX31_RS20705 at 783.335 kb on + strand, within EX31_RS20705 at 783.336 kb on - strand, within EX31_RS20705 at 783.336 kb on - strand, within EX31_RS20705 at 783.336 kb on - strand, within EX31_RS20705 at 783.336 kb on - strand, within EX31_RS20705 at 783.336 kb on - strand, within EX31_RS20705 at 783.336 kb on - strand, within EX31_RS20705 at 783.336 kb on - strand, within EX31_RS20705 at 783.336 kb on - strand, within EX31_RS20705 at 783.336 kb on - strand, within EX31_RS20705 at 783.336 kb on - strand, within EX31_RS20705 at 783.336 kb on - strand, within EX31_RS20705 at 783.336 kb on - strand, within EX31_RS20705 at 783.448 kb on - strand, within EX31_RS20705 at 783.522 kb on + strand, within EX31_RS20705 at 783.525 kb on + strand, within EX31_RS20705 at 783.582 kb on + strand, within EX31_RS20705 at 783.583 kb on - strand, within EX31_RS20705 at 783.583 kb on - strand, within EX31_RS20705 at 783.583 kb on - strand, within EX31_RS20705 at 783.583 kb on - strand, within EX31_RS20705 at 783.583 kb on - strand, within EX31_RS20705 at 783.583 kb on - strand, within EX31_RS20705 at 783.587 kb on + strand, within EX31_RS20705 at 783.587 kb on + strand, within EX31_RS20705 at 783.587 kb on + strand, within EX31_RS20705 at 783.588 kb on - strand, within EX31_RS20705 at 783.588 kb on - strand, within EX31_RS20705 at 783.588 kb on - strand, within EX31_RS20705 at 783.588 kb on - strand, within EX31_RS20705 at 783.588 kb on - strand, within EX31_RS20705 at 783.599 kb on + strand, within EX31_RS20705 at 783.600 kb on - strand, within EX31_RS20705 at 783.620 kb on - strand, within EX31_RS20705 at 783.620 kb on - strand, within EX31_RS20705 at 783.620 kb on - strand, within EX31_RS20705 at 783.620 kb on - strand, within EX31_RS20705 at 783.620 kb on - strand, within EX31_RS20705 at 783.769 kb on - strand, within EX31_RS20705 at 783.769 kb on - strand, within EX31_RS20705 at 783.769 kb on - strand, within EX31_RS20705 at 783.769 kb on - strand, within EX31_RS20705 at 783.794 kb on - strand, within EX31_RS20705 at 783.794 kb on - strand, within EX31_RS20705 at 783.794 kb on - strand, within EX31_RS20705 at 784.003 kb on - strand, within EX31_RS20705 at 784.027 kb on + strand at 784.028 kb on - strand at 784.072 kb on + strand at 784.072 kb on + strand at 784.072 kb on + strand at 784.073 kb on - strand at 784.080 kb on + strand at 784.081 kb on - strand at 784.105 kb on + strand at 784.106 kb on - strand at 784.106 kb on - strand at 784.136 kb on + strand at 784.137 kb on - strand at 784.137 kb on - strand at 784.137 kb on - strand at 784.137 kb on - strand at 784.138 kb on + strand at 784.138 kb on + strand at 784.138 kb on + strand at 784.138 kb on + strand at 784.138 kb on + strand at 784.138 kb on + strand at 784.138 kb on + strand at 784.138 kb on + strand at 784.139 kb on - strand at 784.139 kb on - strand at 784.139 kb on - strand at 784.139 kb on - strand at 784.139 kb on - strand at 784.139 kb on - strand at 784.139 kb on - strand at 784.142 kb on - strand at 784.151 kb on + strand at 784.151 kb on + strand at 784.152 kb on - strand at 784.157 kb on - strand at 784.182 kb on + strand at 784.183 kb on - strand at 784.183 kb on - strand at 784.183 kb on - strand at 784.192 kb on + strand at 784.192 kb on + strand at 784.193 kb on - strand at 784.193 kb on - strand at 784.213 kb on + strand at 784.269 kb on + strand at 784.330 kb on + strand at 784.386 kb on + strand, within EX31_RS20710 at 784.387 kb on - strand, within EX31_RS20710 at 784.480 kb on - strand, within EX31_RS20710 at 784.545 kb on - strand, within EX31_RS20710 at 784.553 kb on + strand, within EX31_RS20710 at 784.554 kb on - strand, within EX31_RS20710 at 784.554 kb on - strand, within EX31_RS20710 at 784.554 kb on - strand, within EX31_RS20710 at 784.554 kb on - strand, within EX31_RS20710 at 784.556 kb on + strand, within EX31_RS20710 at 784.556 kb on + strand, within EX31_RS20710 at 784.556 kb on + strand, within EX31_RS20710 at 784.556 kb on + strand, within EX31_RS20710 at 784.556 kb on + strand, within EX31_RS20710 at 784.556 kb on + strand, within EX31_RS20710 at 784.556 kb on + strand, within EX31_RS20710 at 784.557 kb on - strand, within EX31_RS20710 at 784.557 kb on - strand, within EX31_RS20710 at 784.557 kb on - strand, within EX31_RS20710 at 784.557 kb on - strand, within EX31_RS20710 at 784.557 kb on - strand, within EX31_RS20710 at 784.557 kb on - strand, within EX31_RS20710 at 784.557 kb on - strand, within EX31_RS20710 at 784.557 kb on - strand, within EX31_RS20710 at 784.557 kb on - strand, within EX31_RS20710 at 784.563 kb on + strand, within EX31_RS20710 at 784.579 kb on + strand, within EX31_RS20710 at 784.612 kb on + strand, within EX31_RS20710 at 784.612 kb on + strand, within EX31_RS20710 at 784.613 kb on - strand, within EX31_RS20710 at 784.702 kb on + strand, within EX31_RS20710 at 784.702 kb on + strand, within EX31_RS20710 at 784.703 kb on - strand, within EX31_RS20710 at 784.703 kb on - strand, within EX31_RS20710 at 784.704 kb on + strand, within EX31_RS20710 at 784.704 kb on + strand, within EX31_RS20710 at 784.704 kb on + strand, within EX31_RS20710 at 784.704 kb on + strand, within EX31_RS20710 at 784.704 kb on + strand, within EX31_RS20710 at 784.704 kb on + strand, within EX31_RS20710 at 784.704 kb on + strand, within EX31_RS20710 at 784.705 kb on - strand, within EX31_RS20710 at 784.705 kb on - strand, within EX31_RS20710 at 784.705 kb on - strand, within EX31_RS20710 at 784.796 kb on + strand, within EX31_RS20710 at 784.876 kb on + strand, within EX31_RS20710 at 784.876 kb on + strand, within EX31_RS20710 at 784.876 kb on + strand, within EX31_RS20710 at 784.877 kb on - strand, within EX31_RS20710 at 784.877 kb on - strand, within EX31_RS20710 at 784.941 kb on + strand, within EX31_RS20710 at 784.942 kb on - strand, within EX31_RS20710 at 784.958 kb on - strand, within EX31_RS20710 at 784.991 kb on - strand, within EX31_RS20710 at 784.991 kb on - strand, within EX31_RS20710 at 785.010 kb on + strand, within EX31_RS20710 at 785.033 kb on + strand, within EX31_RS20710 at 785.033 kb on + strand, within EX31_RS20710 at 785.034 kb on - strand, within EX31_RS20710 at 785.034 kb on - strand, within EX31_RS20710 at 785.034 kb on - strand, within EX31_RS20710
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 782,231 + EX31_RS20695 0.31 +0.4 782,231 + EX31_RS20695 0.31 +0.3 782,231 + EX31_RS20695 0.31 +2.7 782,231 + EX31_RS20695 0.31 -0.7 782,309 - EX31_RS20695 0.47 +0.1 782,315 + EX31_RS20695 0.48 -0.3 782,315 + EX31_RS20695 0.48 +0.3 782,329 + EX31_RS20695 0.51 +1.1 782,404 - EX31_RS20695 0.67 -0.1 782,437 + EX31_RS20695 0.74 -0.7 782,437 + EX31_RS20695 0.74 +0.7 782,437 + EX31_RS20695 0.74 +0.0 782,438 - EX31_RS20695 0.74 +0.6 782,438 - EX31_RS20695 0.74 -0.6 782,438 - EX31_RS20695 0.74 -0.1 782,576 - +0.7 782,701 - -0.2 782,758 - EX31_RS20700 0.16 +0.0 782,775 - EX31_RS20700 0.20 -0.3 782,867 - EX31_RS20700 0.39 +0.3 782,885 + EX31_RS20700 0.42 -0.6 783,152 - +0.2 783,152 - -0.6 783,152 - -0.7 783,181 + +0.4 783,181 + -0.2 783,182 - -0.5 783,182 - +1.0 783,182 - +0.3 783,182 - +0.0 783,182 - -0.2 783,185 + -0.5 783,197 + -0.5 783,198 - -2.2 783,198 - -0.1 783,198 - +0.5 783,198 - -0.3 783,208 + +0.7 783,208 + +0.0 783,208 + +0.1 783,209 - -0.1 783,209 - +0.0 783,209 - +0.1 783,209 - -0.8 783,285 + -1.4 783,285 + -1.7 783,285 + -0.5 783,285 + +0.4 783,285 + -0.1 783,285 + +1.0 783,286 - -0.1 783,286 - +1.8 783,286 - -0.1 783,286 - +0.2 783,286 - +1.0 783,286 - +0.1 783,286 - +0.2 783,286 - +0.7 783,286 - -0.3 783,287 + EX31_RS20705 0.10 +0.4 783,288 - EX31_RS20705 0.10 -0.0 783,304 + EX31_RS20705 0.12 +1.0 783,305 - EX31_RS20705 0.12 +0.9 783,305 - EX31_RS20705 0.12 +0.1 783,305 - EX31_RS20705 0.12 -0.6 783,311 + EX31_RS20705 0.13 +1.3 783,312 - EX31_RS20705 0.13 -1.8 783,335 + EX31_RS20705 0.15 +0.5 783,335 + EX31_RS20705 0.15 -0.0 783,335 + EX31_RS20705 0.15 -0.3 783,336 - EX31_RS20705 0.15 +0.3 783,336 - EX31_RS20705 0.15 -0.6 783,336 - EX31_RS20705 0.15 +0.9 783,336 - EX31_RS20705 0.15 +1.0 783,336 - EX31_RS20705 0.15 +0.1 783,336 - EX31_RS20705 0.15 -0.3 783,336 - EX31_RS20705 0.15 +0.0 783,336 - EX31_RS20705 0.15 -0.5 783,336 - EX31_RS20705 0.15 -0.1 783,336 - EX31_RS20705 0.15 -0.3 783,336 - EX31_RS20705 0.15 -0.2 783,336 - EX31_RS20705 0.15 -0.9 783,448 - EX31_RS20705 0.28 +0.3 783,522 + EX31_RS20705 0.36 -0.8 783,525 + EX31_RS20705 0.36 -0.3 783,582 + EX31_RS20705 0.42 -1.0 783,583 - EX31_RS20705 0.43 +1.0 783,583 - EX31_RS20705 0.43 -0.6 783,583 - EX31_RS20705 0.43 -0.9 783,583 - EX31_RS20705 0.43 -0.1 783,583 - EX31_RS20705 0.43 -0.3 783,583 - EX31_RS20705 0.43 -0.2 783,587 + EX31_RS20705 0.43 -0.5 783,587 + EX31_RS20705 0.43 +0.0 783,587 + EX31_RS20705 0.43 +1.3 783,588 - EX31_RS20705 0.43 -0.9 783,588 - EX31_RS20705 0.43 -0.3 783,588 - EX31_RS20705 0.43 -0.3 783,588 - EX31_RS20705 0.43 -0.4 783,588 - EX31_RS20705 0.43 -0.3 783,599 + EX31_RS20705 0.44 +1.0 783,600 - EX31_RS20705 0.44 -1.2 783,620 - EX31_RS20705 0.47 +0.8 783,620 - EX31_RS20705 0.47 +1.0 783,620 - EX31_RS20705 0.47 +0.2 783,620 - EX31_RS20705 0.47 -1.1 783,620 - EX31_RS20705 0.47 +0.4 783,769 - EX31_RS20705 0.63 -0.8 783,769 - EX31_RS20705 0.63 -0.2 783,769 - EX31_RS20705 0.63 +0.0 783,769 - EX31_RS20705 0.63 +0.3 783,794 - EX31_RS20705 0.66 -0.7 783,794 - EX31_RS20705 0.66 -0.7 783,794 - EX31_RS20705 0.66 +0.4 784,003 - EX31_RS20705 0.89 -0.1 784,027 + -0.2 784,028 - -0.7 784,072 + -1.5 784,072 + +0.5 784,072 + +1.0 784,073 - -0.6 784,080 + +0.9 784,081 - -0.6 784,105 + +2.5 784,106 - -0.1 784,106 - +1.6 784,136 + +0.7 784,137 - +0.7 784,137 - -0.1 784,137 - +0.2 784,137 - +0.0 784,138 + +1.6 784,138 + +0.6 784,138 + +0.1 784,138 + +0.0 784,138 + -0.7 784,138 + -1.1 784,138 + +0.5 784,138 + +0.6 784,139 - -0.0 784,139 - -1.3 784,139 - -0.3 784,139 - -0.5 784,139 - +0.2 784,139 - -0.5 784,139 - -0.5 784,142 - +0.7 784,151 + -0.9 784,151 + -1.0 784,152 - -0.2 784,157 - -1.0 784,182 + +0.5 784,183 - +1.4 784,183 - -0.7 784,183 - -0.7 784,192 + +0.8 784,192 + +0.7 784,193 - +0.3 784,193 - +0.9 784,213 + +0.3 784,269 + -0.1 784,330 + -0.2 784,386 + EX31_RS20710 0.14 -0.9 784,387 - EX31_RS20710 0.15 +0.0 784,480 - EX31_RS20710 0.23 +0.2 784,545 - EX31_RS20710 0.28 +0.3 784,553 + EX31_RS20710 0.29 -0.2 784,554 - EX31_RS20710 0.29 +1.0 784,554 - EX31_RS20710 0.29 -0.7 784,554 - EX31_RS20710 0.29 +0.2 784,554 - EX31_RS20710 0.29 +0.6 784,556 + EX31_RS20710 0.29 +0.4 784,556 + EX31_RS20710 0.29 +0.4 784,556 + EX31_RS20710 0.29 +0.2 784,556 + EX31_RS20710 0.29 -0.1 784,556 + EX31_RS20710 0.29 -0.2 784,556 + EX31_RS20710 0.29 +0.0 784,556 + EX31_RS20710 0.29 -0.4 784,557 - EX31_RS20710 0.29 +0.8 784,557 - EX31_RS20710 0.29 +1.0 784,557 - EX31_RS20710 0.29 -0.3 784,557 - EX31_RS20710 0.29 +0.7 784,557 - EX31_RS20710 0.29 +1.2 784,557 - EX31_RS20710 0.29 -0.1 784,557 - EX31_RS20710 0.29 +0.5 784,557 - EX31_RS20710 0.29 -0.1 784,557 - EX31_RS20710 0.29 +1.7 784,563 + EX31_RS20710 0.30 +0.3 784,579 + EX31_RS20710 0.31 -0.3 784,612 + EX31_RS20710 0.34 +0.5 784,612 + EX31_RS20710 0.34 +0.9 784,613 - EX31_RS20710 0.34 +2.0 784,702 + EX31_RS20710 0.42 -0.4 784,702 + EX31_RS20710 0.42 -0.1 784,703 - EX31_RS20710 0.42 +0.0 784,703 - EX31_RS20710 0.42 -0.7 784,704 + EX31_RS20710 0.42 +0.0 784,704 + EX31_RS20710 0.42 +1.4 784,704 + EX31_RS20710 0.42 -0.0 784,704 + EX31_RS20710 0.42 -1.1 784,704 + EX31_RS20710 0.42 +0.0 784,704 + EX31_RS20710 0.42 +0.6 784,704 + EX31_RS20710 0.42 -0.6 784,705 - EX31_RS20710 0.42 -0.2 784,705 - EX31_RS20710 0.42 -1.6 784,705 - EX31_RS20710 0.42 +0.3 784,796 + EX31_RS20710 0.50 +0.5 784,876 + EX31_RS20710 0.57 -0.5 784,876 + EX31_RS20710 0.57 +0.3 784,876 + EX31_RS20710 0.57 +0.1 784,877 - EX31_RS20710 0.57 -0.0 784,877 - EX31_RS20710 0.57 +0.7 784,941 + EX31_RS20710 0.63 +0.5 784,942 - EX31_RS20710 0.63 +0.4 784,958 - EX31_RS20710 0.64 +1.2 784,991 - EX31_RS20710 0.67 -1.0 784,991 - EX31_RS20710 0.67 -0.1 785,010 + EX31_RS20710 0.69 -0.9 785,033 + EX31_RS20710 0.71 +0.4 785,033 + EX31_RS20710 0.71 +0.9 785,034 - EX31_RS20710 0.71 -0.6 785,034 - EX31_RS20710 0.71 +0.6 785,034 - EX31_RS20710 0.71 -0.6
Or see this region's nucleotide sequence