Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20170 and EX31_RS20175 are separated by 210 nucleotides EX31_RS20175 and EX31_RS20180 are separated by 403 nucleotides EX31_RS20180 and EX31_RS20185 are separated by 50 nucleotides
EX31_RS20170: EX31_RS20170 - sulfate transporter CysZ, at 662,426 to 663,226
_RS20170
EX31_RS20175: EX31_RS20175 - cysteine synthase A, at 663,437 to 664,402
_RS20175
EX31_RS20180: EX31_RS20180 - phosphocarrier protein Hpr, at 664,806 to 665,063
_RS20180
EX31_RS20185: EX31_RS20185 - phosphoenolpyruvate-protein phosphotransferase PtsI, at 665,114 to 666,841
_RS20185
Position (kb) 663
664
665 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 662.468 kb on + strand at 662.468 kb on + strand at 662.468 kb on + strand at 662.469 kb on - strand at 662.526 kb on + strand, within EX31_RS20170 at 662.645 kb on + strand, within EX31_RS20170 at 662.645 kb on + strand, within EX31_RS20170 at 662.646 kb on - strand, within EX31_RS20170 at 662.693 kb on + strand, within EX31_RS20170 at 662.693 kb on + strand, within EX31_RS20170 at 662.693 kb on + strand, within EX31_RS20170 at 662.693 kb on + strand, within EX31_RS20170 at 662.694 kb on - strand, within EX31_RS20170 at 662.694 kb on - strand, within EX31_RS20170 at 662.694 kb on - strand, within EX31_RS20170 at 662.694 kb on - strand, within EX31_RS20170 at 662.792 kb on - strand, within EX31_RS20170 at 662.821 kb on - strand, within EX31_RS20170 at 662.858 kb on + strand, within EX31_RS20170 at 662.859 kb on - strand, within EX31_RS20170 at 662.861 kb on + strand, within EX31_RS20170 at 662.861 kb on + strand, within EX31_RS20170 at 662.861 kb on + strand, within EX31_RS20170 at 662.862 kb on - strand, within EX31_RS20170 at 662.862 kb on - strand, within EX31_RS20170 at 662.895 kb on - strand, within EX31_RS20170 at 662.895 kb on - strand, within EX31_RS20170 at 662.895 kb on - strand, within EX31_RS20170 at 662.899 kb on + strand, within EX31_RS20170 at 662.972 kb on + strand, within EX31_RS20170 at 663.083 kb on - strand, within EX31_RS20170 at 663.166 kb on + strand at 663.166 kb on + strand at 663.166 kb on + strand at 663.171 kb on - strand at 663.171 kb on - strand at 663.171 kb on - strand at 663.176 kb on - strand at 663.188 kb on + strand at 663.188 kb on + strand at 663.188 kb on + strand at 663.188 kb on + strand at 663.188 kb on + strand at 663.188 kb on + strand at 663.188 kb on + strand at 663.189 kb on - strand at 663.189 kb on - strand at 663.189 kb on - strand at 663.189 kb on - strand at 663.189 kb on - strand at 663.189 kb on - strand at 663.189 kb on - strand at 663.190 kb on + strand at 663.190 kb on + strand at 663.190 kb on + strand at 663.191 kb on - strand at 663.191 kb on - strand at 663.196 kb on + strand at 663.196 kb on + strand at 663.196 kb on + strand at 663.196 kb on + strand at 663.196 kb on + strand at 663.196 kb on + strand at 663.197 kb on - strand at 663.197 kb on - strand at 663.197 kb on - strand at 663.197 kb on - strand at 663.197 kb on - strand at 663.197 kb on - strand at 663.199 kb on + strand at 663.199 kb on + strand at 663.199 kb on + strand at 663.200 kb on - strand at 663.234 kb on + strand at 663.265 kb on + strand at 663.265 kb on + strand at 663.265 kb on + strand at 663.266 kb on - strand at 663.266 kb on - strand at 663.266 kb on - strand at 663.266 kb on - strand at 663.266 kb on - strand at 663.266 kb on - strand at 663.266 kb on - strand at 663.266 kb on - strand at 663.291 kb on + strand at 663.291 kb on + strand at 663.291 kb on + strand at 663.292 kb on - strand at 663.292 kb on - strand at 663.298 kb on + strand at 663.298 kb on + strand at 663.301 kb on + strand at 663.301 kb on + strand at 663.302 kb on - strand at 663.302 kb on - strand at 663.302 kb on - strand at 663.313 kb on - strand at 663.313 kb on - strand at 663.313 kb on - strand at 663.314 kb on + strand at 663.314 kb on + strand at 663.314 kb on + strand at 663.314 kb on + strand at 663.314 kb on + strand at 663.315 kb on - strand at 663.315 kb on - strand at 663.315 kb on - strand at 663.315 kb on - strand at 663.315 kb on - strand at 663.321 kb on + strand at 663.322 kb on - strand at 663.332 kb on + strand at 663.333 kb on - strand at 663.333 kb on - strand at 663.357 kb on + strand at 663.418 kb on + strand at 663.418 kb on + strand at 663.418 kb on + strand at 663.419 kb on - strand at 663.424 kb on + strand at 663.424 kb on + strand at 663.424 kb on + strand at 663.425 kb on - strand at 663.437 kb on - strand at 663.437 kb on - strand at 663.449 kb on + strand at 663.449 kb on + strand at 663.449 kb on + strand at 663.450 kb on - strand at 663.450 kb on - strand at 663.450 kb on - strand at 663.450 kb on - strand at 663.450 kb on - strand at 663.541 kb on + strand, within EX31_RS20175 at 663.541 kb on + strand, within EX31_RS20175 at 663.542 kb on - strand, within EX31_RS20175 at 663.622 kb on + strand, within EX31_RS20175 at 663.640 kb on + strand, within EX31_RS20175 at 663.640 kb on + strand, within EX31_RS20175 at 663.640 kb on + strand, within EX31_RS20175 at 663.640 kb on + strand, within EX31_RS20175 at 663.640 kb on + strand, within EX31_RS20175 at 663.640 kb on + strand, within EX31_RS20175 at 663.671 kb on + strand, within EX31_RS20175 at 663.671 kb on + strand, within EX31_RS20175 at 663.671 kb on + strand, within EX31_RS20175 at 663.672 kb on - strand, within EX31_RS20175 at 663.672 kb on - strand, within EX31_RS20175 at 663.710 kb on - strand, within EX31_RS20175 at 663.710 kb on - strand, within EX31_RS20175 at 663.710 kb on - strand, within EX31_RS20175 at 663.739 kb on + strand, within EX31_RS20175 at 663.739 kb on + strand, within EX31_RS20175 at 663.848 kb on + strand, within EX31_RS20175 at 663.848 kb on + strand, within EX31_RS20175 at 663.853 kb on + strand, within EX31_RS20175 at 663.853 kb on + strand, within EX31_RS20175 at 663.854 kb on - strand, within EX31_RS20175 at 663.854 kb on - strand, within EX31_RS20175 at 663.928 kb on + strand, within EX31_RS20175 at 663.928 kb on + strand, within EX31_RS20175 at 663.980 kb on - strand, within EX31_RS20175 at 663.980 kb on - strand, within EX31_RS20175 at 664.106 kb on - strand, within EX31_RS20175 at 664.132 kb on + strand, within EX31_RS20175 at 664.176 kb on + strand, within EX31_RS20175 at 664.374 kb on + strand at 664.375 kb on - strand at 664.400 kb on + strand at 664.400 kb on + strand at 664.400 kb on + strand at 664.414 kb on + strand at 664.415 kb on - strand at 664.471 kb on + strand at 664.471 kb on + strand at 664.471 kb on + strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.472 kb on - strand at 664.506 kb on + strand at 664.507 kb on - strand at 664.507 kb on - strand at 664.510 kb on - strand at 664.510 kb on - strand at 664.513 kb on + strand at 664.514 kb on - strand at 664.612 kb on + strand at 664.612 kb on + strand at 664.613 kb on - strand at 664.623 kb on + strand at 664.624 kb on - strand at 664.624 kb on - strand at 664.734 kb on - strand at 664.740 kb on + strand at 664.741 kb on - strand at 664.754 kb on + strand at 664.755 kb on - strand at 664.759 kb on + strand at 664.766 kb on + strand at 664.766 kb on + strand at 664.766 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.780 kb on + strand at 664.781 kb on - strand at 664.781 kb on - strand at 664.781 kb on - strand at 664.781 kb on - strand at 664.785 kb on + strand at 664.785 kb on + strand at 664.785 kb on + strand at 664.785 kb on + strand at 664.786 kb on - strand at 664.788 kb on + strand at 664.788 kb on + strand at 664.788 kb on + strand at 664.789 kb on - strand at 664.789 kb on - strand at 664.789 kb on - strand at 664.823 kb on + strand at 664.823 kb on + strand at 664.823 kb on + strand at 664.823 kb on + strand at 664.826 kb on + strand at 664.826 kb on + strand at 664.826 kb on + strand at 664.826 kb on + strand at 664.829 kb on + strand at 664.838 kb on + strand, within EX31_RS20180 at 664.838 kb on + strand, within EX31_RS20180 at 664.937 kb on + strand, within EX31_RS20180 at 664.937 kb on + strand, within EX31_RS20180 at 665.038 kb on + strand at 665.061 kb on + strand at 665.061 kb on + strand at 665.065 kb on - strand at 665.065 kb on - strand at 665.078 kb on + strand at 665.078 kb on + strand at 665.078 kb on + strand at 665.081 kb on - strand at 665.107 kb on + strand at 665.107 kb on + strand at 665.107 kb on + strand at 665.113 kb on + strand at 665.382 kb on + strand, within EX31_RS20185
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 662,468 + -0.5 662,468 + -0.1 662,468 + -1.0 662,469 - +0.3 662,526 + EX31_RS20170 0.12 +0.3 662,645 + EX31_RS20170 0.27 +0.2 662,645 + EX31_RS20170 0.27 +0.2 662,646 - EX31_RS20170 0.27 +0.5 662,693 + EX31_RS20170 0.33 -0.3 662,693 + EX31_RS20170 0.33 -0.5 662,693 + EX31_RS20170 0.33 +0.7 662,693 + EX31_RS20170 0.33 +0.4 662,694 - EX31_RS20170 0.33 -1.6 662,694 - EX31_RS20170 0.33 -0.8 662,694 - EX31_RS20170 0.33 -0.0 662,694 - EX31_RS20170 0.33 -1.5 662,792 - EX31_RS20170 0.46 +2.1 662,821 - EX31_RS20170 0.49 -1.6 662,858 + EX31_RS20170 0.54 +1.1 662,859 - EX31_RS20170 0.54 -0.3 662,861 + EX31_RS20170 0.54 +0.2 662,861 + EX31_RS20170 0.54 -0.9 662,861 + EX31_RS20170 0.54 +1.0 662,862 - EX31_RS20170 0.54 -1.1 662,862 - EX31_RS20170 0.54 +0.1 662,895 - EX31_RS20170 0.59 -1.4 662,895 - EX31_RS20170 0.59 +0.3 662,895 - EX31_RS20170 0.59 +1.0 662,899 + EX31_RS20170 0.59 +0.9 662,972 + EX31_RS20170 0.68 +0.5 663,083 - EX31_RS20170 0.82 -0.6 663,166 + +0.0 663,166 + -0.0 663,166 + -0.1 663,171 - -0.0 663,171 - -1.6 663,171 - +0.1 663,176 - +0.6 663,188 + +0.5 663,188 + +0.4 663,188 + -0.2 663,188 + -0.9 663,188 + +0.6 663,188 + -1.5 663,188 + -0.6 663,189 - -2.2 663,189 - +0.4 663,189 - -0.2 663,189 - -0.3 663,189 - +0.4 663,189 - +0.3 663,189 - -0.0 663,190 + -0.7 663,190 + +0.0 663,190 + +0.4 663,191 - -0.3 663,191 - -0.1 663,196 + -0.2 663,196 + +0.0 663,196 + +0.1 663,196 + +0.0 663,196 + -0.5 663,196 + +0.1 663,197 - +1.1 663,197 - +0.4 663,197 - +1.8 663,197 - +2.0 663,197 - +0.0 663,197 - +1.0 663,199 + +1.7 663,199 + -0.8 663,199 + +1.6 663,200 - +0.6 663,234 + -0.0 663,265 + -1.4 663,265 + -0.1 663,265 + +1.0 663,266 - +0.3 663,266 - -0.3 663,266 - -2.1 663,266 - -0.6 663,266 - -0.2 663,266 - +0.2 663,266 - -1.7 663,266 - +2.0 663,291 + +2.2 663,291 + -0.4 663,291 + +0.8 663,292 - -1.9 663,292 - -2.6 663,298 + -0.6 663,298 + -1.1 663,301 + -0.1 663,301 + +1.4 663,302 - -1.6 663,302 - +2.0 663,302 - -2.8 663,313 - -2.4 663,313 - -2.2 663,313 - -2.9 663,314 + +0.2 663,314 + -0.4 663,314 + +0.4 663,314 + -0.6 663,314 + +0.9 663,315 - -1.5 663,315 - -1.7 663,315 - -3.3 663,315 - -3.2 663,315 - -2.5 663,321 + -0.3 663,322 - -1.2 663,332 + +0.8 663,333 - -3.0 663,333 - -0.6 663,357 + +0.4 663,418 + -0.6 663,418 + -2.2 663,418 + -3.0 663,419 - -2.0 663,424 + -1.8 663,424 + -1.6 663,424 + -0.6 663,425 - -4.5 663,437 - -3.7 663,437 - -1.5 663,449 + -3.0 663,449 + -2.7 663,449 + -0.8 663,450 - -2.0 663,450 - -2.3 663,450 - -0.4 663,450 - -1.7 663,450 - +1.0 663,541 + EX31_RS20175 0.11 -2.1 663,541 + EX31_RS20175 0.11 -3.1 663,542 - EX31_RS20175 0.11 -1.5 663,622 + EX31_RS20175 0.19 -1.0 663,640 + EX31_RS20175 0.21 -3.0 663,640 + EX31_RS20175 0.21 -2.7 663,640 + EX31_RS20175 0.21 -2.1 663,640 + EX31_RS20175 0.21 -3.2 663,640 + EX31_RS20175 0.21 -2.9 663,640 + EX31_RS20175 0.21 -0.3 663,671 + EX31_RS20175 0.24 -3.0 663,671 + EX31_RS20175 0.24 -1.8 663,671 + EX31_RS20175 0.24 -2.2 663,672 - EX31_RS20175 0.24 -0.6 663,672 - EX31_RS20175 0.24 -1.8 663,710 - EX31_RS20175 0.28 -3.1 663,710 - EX31_RS20175 0.28 -1.7 663,710 - EX31_RS20175 0.28 -1.7 663,739 + EX31_RS20175 0.31 -3.2 663,739 + EX31_RS20175 0.31 -3.2 663,848 + EX31_RS20175 0.43 -2.3 663,848 + EX31_RS20175 0.43 -3.5 663,853 + EX31_RS20175 0.43 -1.7 663,853 + EX31_RS20175 0.43 -3.3 663,854 - EX31_RS20175 0.43 -3.3 663,854 - EX31_RS20175 0.43 -1.7 663,928 + EX31_RS20175 0.51 -1.1 663,928 + EX31_RS20175 0.51 -3.9 663,980 - EX31_RS20175 0.56 -2.7 663,980 - EX31_RS20175 0.56 -2.4 664,106 - EX31_RS20175 0.69 -2.3 664,132 + EX31_RS20175 0.72 -1.5 664,176 + EX31_RS20175 0.77 -2.4 664,374 + +2.4 664,375 - -1.3 664,400 + -0.2 664,400 + -0.6 664,400 + +0.8 664,414 + -0.0 664,415 - -0.5 664,471 + -0.2 664,471 + -1.6 664,471 + +0.1 664,472 - -0.6 664,472 - +0.6 664,472 - -0.3 664,472 - +0.0 664,506 + +0.8 664,507 - -1.2 664,507 - -1.1 664,510 - -0.2 664,510 - -1.2 664,513 + -0.4 664,514 - -1.0 664,612 + +0.6 664,612 + -0.6 664,613 - -0.7 664,623 + +0.6 664,624 - +0.6 664,624 - -0.7 664,734 - +0.1 664,740 + -1.9 664,741 - -2.5 664,754 + +0.5 664,755 - +0.4 664,759 + +0.6 664,766 + -0.3 664,766 + -1.9 664,766 + -2.0 664,780 + +0.3 664,780 + -1.0 664,780 + -2.5 664,780 + -1.2 664,780 + -0.6 664,780 + -0.6 664,780 + -1.0 664,780 + -1.5 664,780 + -1.2 664,780 + -3.0 664,781 - -0.0 664,781 - -1.8 664,781 - -1.5 664,781 - -0.8 664,785 + -1.7 664,785 + -1.0 664,785 + -0.7 664,785 + -1.2 664,786 - -0.4 664,788 + -1.5 664,788 + -1.4 664,788 + -1.9 664,789 - +0.3 664,789 - +0.6 664,789 - -0.1 664,823 + -0.8 664,823 + +1.0 664,823 + -0.4 664,823 + +0.5 664,826 + -0.2 664,826 + +0.2 664,826 + -1.9 664,826 + -2.6 664,829 + +1.0 664,838 + EX31_RS20180 0.12 -2.0 664,838 + EX31_RS20180 0.12 +0.3 664,937 + EX31_RS20180 0.51 -1.6 664,937 + EX31_RS20180 0.51 -0.5 665,038 + -0.2 665,061 + +0.1 665,061 + -0.3 665,065 - -1.0 665,065 - -1.2 665,078 + -0.3 665,078 + +2.0 665,078 + -0.2 665,081 - -1.4 665,107 + -1.4 665,107 + -0.6 665,107 + -1.0 665,113 + +0.1 665,382 + EX31_RS20185 0.16 +0.2
Or see this region's nucleotide sequence