Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09940 and EX31_RS09945 are separated by 198 nucleotides EX31_RS09945 and EX31_RS09950 are separated by 34 nucleotides EX31_RS09950 and EX31_RS09955 are separated by 490 nucleotides
EX31_RS09940: EX31_RS09940 - 23S rRNA pseudouridine(2605) synthase RluB, at 196,397 to 197,716
_RS09940
EX31_RS09945: EX31_RS09945 - threonylcarbamoyl-AMP synthase, at 197,915 to 198,535
_RS09945
EX31_RS09950: EX31_RS09950 - PHP domain-containing protein, at 198,570 to 199,493
_RS09950
EX31_RS09955: EX31_RS09955 - anthranilate synthase component 1, at 199,984 to 201,546
_RS09955
Position (kb) 198
199
200 Strain fitness (log2 ratio) -2
-1
0
1
2 at 197.702 kb on - strand at 197.702 kb on - strand at 197.703 kb on + strand at 197.704 kb on - strand at 197.815 kb on + strand at 197.848 kb on - strand at 197.857 kb on + strand at 197.857 kb on + strand at 197.858 kb on - strand at 197.858 kb on - strand at 197.858 kb on - strand at 197.858 kb on - strand at 197.858 kb on - strand at 197.858 kb on - strand at 197.858 kb on - strand at 197.860 kb on - strand at 198.188 kb on - strand, within EX31_RS09945 at 198.246 kb on + strand, within EX31_RS09945 at 198.247 kb on - strand, within EX31_RS09945 at 198.298 kb on - strand, within EX31_RS09945 at 198.298 kb on - strand, within EX31_RS09945 at 198.298 kb on - strand, within EX31_RS09945 at 198.303 kb on + strand, within EX31_RS09945 at 198.303 kb on + strand, within EX31_RS09945 at 198.303 kb on + strand, within EX31_RS09945 at 198.304 kb on - strand, within EX31_RS09945 at 198.304 kb on - strand, within EX31_RS09945 at 198.347 kb on - strand, within EX31_RS09945 at 198.347 kb on - strand, within EX31_RS09945 at 198.379 kb on + strand, within EX31_RS09945 at 198.379 kb on + strand, within EX31_RS09945 at 198.380 kb on - strand, within EX31_RS09945 at 198.380 kb on - strand, within EX31_RS09945 at 198.484 kb on + strand at 198.517 kb on + strand at 198.518 kb on - strand at 198.518 kb on - strand at 198.526 kb on - strand at 198.546 kb on + strand at 198.547 kb on - strand at 198.631 kb on - strand at 198.631 kb on - strand at 198.730 kb on + strand, within EX31_RS09950 at 198.731 kb on - strand, within EX31_RS09950 at 198.915 kb on + strand, within EX31_RS09950 at 198.915 kb on + strand, within EX31_RS09950 at 198.915 kb on + strand, within EX31_RS09950 at 198.916 kb on - strand, within EX31_RS09950 at 198.916 kb on - strand, within EX31_RS09950 at 198.916 kb on - strand, within EX31_RS09950 at 198.916 kb on - strand, within EX31_RS09950 at 198.916 kb on - strand, within EX31_RS09950 at 198.921 kb on - strand, within EX31_RS09950 at 198.921 kb on - strand, within EX31_RS09950 at 199.063 kb on - strand, within EX31_RS09950 at 199.141 kb on + strand, within EX31_RS09950 at 199.142 kb on - strand, within EX31_RS09950 at 199.142 kb on - strand, within EX31_RS09950 at 199.158 kb on + strand, within EX31_RS09950 at 199.159 kb on - strand, within EX31_RS09950 at 199.205 kb on + strand, within EX31_RS09950 at 199.206 kb on - strand, within EX31_RS09950 at 199.252 kb on - strand, within EX31_RS09950 at 199.357 kb on - strand, within EX31_RS09950 at 199.390 kb on + strand, within EX31_RS09950 at 199.391 kb on - strand, within EX31_RS09950 at 199.409 kb on + strand at 199.410 kb on - strand at 199.410 kb on - strand at 199.426 kb on + strand at 199.433 kb on + strand at 199.434 kb on - strand at 199.434 kb on - strand at 199.533 kb on + strand at 199.533 kb on + strand at 199.533 kb on + strand at 199.534 kb on - strand at 199.534 kb on - strand at 199.534 kb on - strand at 199.534 kb on - strand at 199.534 kb on - strand at 199.534 kb on - strand at 199.538 kb on + strand at 199.595 kb on - strand at 199.602 kb on + strand at 199.602 kb on + strand at 199.603 kb on - strand at 199.603 kb on - strand at 199.603 kb on - strand at 199.603 kb on - strand at 199.603 kb on - strand at 199.604 kb on + strand at 199.604 kb on + strand at 199.604 kb on + strand at 199.653 kb on + strand at 199.691 kb on + strand at 199.711 kb on + strand at 199.711 kb on + strand at 199.754 kb on - strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.769 kb on + strand at 199.770 kb on - strand at 199.809 kb on + strand at 199.809 kb on + strand at 199.809 kb on + strand at 199.839 kb on - strand at 199.860 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.870 kb on + strand at 199.871 kb on - strand at 199.871 kb on - strand at 199.953 kb on + strand at 199.953 kb on + strand at 199.989 kb on + strand at 199.990 kb on - strand at 199.990 kb on - strand at 199.990 kb on - strand at 200.006 kb on + strand at 200.222 kb on + strand, within EX31_RS09955 at 200.474 kb on + strand, within EX31_RS09955 at 200.475 kb on - strand, within EX31_RS09955
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 197,702 - +0.3 197,702 - +1.0 197,703 + -1.3 197,704 - -0.9 197,815 + +0.1 197,848 - +0.7 197,857 + -1.5 197,857 + +1.3 197,858 - -0.6 197,858 - -0.8 197,858 - -0.7 197,858 - +0.8 197,858 - +0.8 197,858 - +0.5 197,858 - -1.9 197,860 - +1.4 198,188 - EX31_RS09945 0.44 -0.5 198,246 + EX31_RS09945 0.53 -0.0 198,247 - EX31_RS09945 0.53 -1.1 198,298 - EX31_RS09945 0.62 +0.3 198,298 - EX31_RS09945 0.62 +0.2 198,298 - EX31_RS09945 0.62 -1.1 198,303 + EX31_RS09945 0.62 -0.2 198,303 + EX31_RS09945 0.62 -0.7 198,303 + EX31_RS09945 0.62 -1.3 198,304 - EX31_RS09945 0.63 +0.4 198,304 - EX31_RS09945 0.63 -1.3 198,347 - EX31_RS09945 0.70 +0.4 198,347 - EX31_RS09945 0.70 +1.6 198,379 + EX31_RS09945 0.75 +0.4 198,379 + EX31_RS09945 0.75 +0.3 198,380 - EX31_RS09945 0.75 +0.3 198,380 - EX31_RS09945 0.75 +1.0 198,484 + +0.4 198,517 + +1.3 198,518 - -0.6 198,518 - -0.8 198,526 - -0.2 198,546 + -1.6 198,547 - +0.1 198,631 - -1.6 198,631 - +0.3 198,730 + EX31_RS09950 0.17 +1.0 198,731 - EX31_RS09950 0.17 +1.7 198,915 + EX31_RS09950 0.37 +1.9 198,915 + EX31_RS09950 0.37 +0.9 198,915 + EX31_RS09950 0.37 +0.6 198,916 - EX31_RS09950 0.37 -0.0 198,916 - EX31_RS09950 0.37 -2.1 198,916 - EX31_RS09950 0.37 -0.2 198,916 - EX31_RS09950 0.37 -0.1 198,916 - EX31_RS09950 0.37 +1.0 198,921 - EX31_RS09950 0.38 -1.3 198,921 - EX31_RS09950 0.38 +1.5 199,063 - EX31_RS09950 0.53 -0.8 199,141 + EX31_RS09950 0.62 -0.3 199,142 - EX31_RS09950 0.62 +1.0 199,142 - EX31_RS09950 0.62 -1.8 199,158 + EX31_RS09950 0.64 +0.6 199,159 - EX31_RS09950 0.64 +0.8 199,205 + EX31_RS09950 0.69 +0.4 199,206 - EX31_RS09950 0.69 -0.3 199,252 - EX31_RS09950 0.74 +0.1 199,357 - EX31_RS09950 0.85 +0.3 199,390 + EX31_RS09950 0.89 +0.5 199,391 - EX31_RS09950 0.89 -1.5 199,409 + +1.1 199,410 - -0.6 199,410 - -0.8 199,426 + -1.1 199,433 + -1.0 199,434 - -0.1 199,434 - -0.9 199,533 + +0.4 199,533 + +0.7 199,533 + -1.3 199,534 - +0.6 199,534 - +1.7 199,534 - -1.1 199,534 - +0.1 199,534 - +0.0 199,534 - -0.4 199,538 + +0.5 199,595 - -0.0 199,602 + -0.3 199,602 + +0.4 199,603 - -0.7 199,603 - -0.1 199,603 - +0.1 199,603 - -0.1 199,603 - +0.1 199,604 + +0.8 199,604 + +0.4 199,604 + +1.7 199,653 + +1.0 199,691 + -1.4 199,711 + -0.7 199,711 + -0.0 199,754 - -0.3 199,769 + -2.7 199,769 + +0.8 199,769 + +0.4 199,770 - -0.3 199,809 + +1.4 199,809 + +0.9 199,809 + +0.1 199,839 - +0.1 199,860 + -0.0 199,870 + +0.0 199,870 + +1.0 199,870 + -1.7 199,870 + -0.9 199,870 + -0.2 199,870 + -2.1 199,870 + +0.1 199,871 - -1.8 199,871 - -2.1 199,953 + -0.5 199,953 + +1.9 199,989 + -2.3 199,990 - -0.3 199,990 - -2.6 199,990 - +0.1 200,006 + -2.2 200,222 + EX31_RS09955 0.15 -1.9 200,474 + EX31_RS09955 0.31 +0.1 200,475 - EX31_RS09955 0.31 -2.5
Or see this region's nucleotide sequence