Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01165 and EX31_RS01170 are separated by 739 nucleotides EX31_RS01170 and EX31_RS01175 are separated by 24 nucleotides EX31_RS01175 and EX31_RS01180 overlap by 7 nucleotides
EX31_RS01165: EX31_RS01165 - anion permease, at 255,562 to 257,073
_RS01165
EX31_RS01170: EX31_RS01170 - flavocytochrome c, at 257,813 to 260,590
_RS01170
EX31_RS01175: EX31_RS01175 - FAD:protein FMN transferase, at 260,615 to 261,577
_RS01175
EX31_RS01180: EX31_RS01180 - two-component system response regulator DcuR, at 261,571 to 262,290
_RS01180
Position (kb) 257
258
259
260
261 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 256.990 kb on + strand at 256.990 kb on + strand at 256.990 kb on + strand at 256.990 kb on + strand at 256.991 kb on - strand at 257.077 kb on - strand at 257.085 kb on + strand at 257.085 kb on + strand at 257.090 kb on - strand at 257.100 kb on - strand at 257.100 kb on - strand at 257.114 kb on + strand at 257.115 kb on - strand at 257.115 kb on - strand at 257.115 kb on - strand at 257.119 kb on - strand at 257.119 kb on - strand at 257.119 kb on - strand at 257.220 kb on - strand at 257.249 kb on - strand at 257.250 kb on + strand at 257.523 kb on - strand at 257.551 kb on + strand at 257.552 kb on - strand at 257.753 kb on + strand at 257.809 kb on + strand at 257.835 kb on + strand at 257.836 kb on - strand at 257.948 kb on + strand at 257.949 kb on - strand at 258.011 kb on - strand at 258.335 kb on + strand, within EX31_RS01170 at 258.336 kb on - strand, within EX31_RS01170 at 258.336 kb on - strand, within EX31_RS01170 at 258.462 kb on - strand, within EX31_RS01170 at 258.545 kb on + strand, within EX31_RS01170 at 258.545 kb on + strand, within EX31_RS01170 at 258.545 kb on + strand, within EX31_RS01170 at 258.659 kb on + strand, within EX31_RS01170 at 258.659 kb on + strand, within EX31_RS01170 at 258.659 kb on + strand, within EX31_RS01170 at 258.660 kb on - strand, within EX31_RS01170 at 258.725 kb on - strand, within EX31_RS01170 at 258.758 kb on + strand, within EX31_RS01170 at 258.758 kb on + strand, within EX31_RS01170 at 258.758 kb on + strand, within EX31_RS01170 at 259.118 kb on - strand, within EX31_RS01170 at 259.256 kb on + strand, within EX31_RS01170 at 259.455 kb on - strand, within EX31_RS01170 at 259.492 kb on + strand, within EX31_RS01170 at 259.672 kb on + strand, within EX31_RS01170 at 259.681 kb on + strand, within EX31_RS01170 at 259.681 kb on + strand, within EX31_RS01170 at 259.681 kb on + strand, within EX31_RS01170 at 259.682 kb on - strand, within EX31_RS01170 at 259.685 kb on - strand, within EX31_RS01170 at 259.851 kb on - strand, within EX31_RS01170 at 259.994 kb on + strand, within EX31_RS01170 at 259.995 kb on - strand, within EX31_RS01170 at 260.127 kb on - strand, within EX31_RS01170 at 260.161 kb on + strand, within EX31_RS01170 at 260.177 kb on + strand, within EX31_RS01170 at 260.177 kb on + strand, within EX31_RS01170 at 260.178 kb on - strand, within EX31_RS01170 at 260.276 kb on + strand, within EX31_RS01170 at 260.276 kb on + strand, within EX31_RS01170 at 260.277 kb on - strand, within EX31_RS01170 at 260.360 kb on + strand at 260.360 kb on + strand at 260.360 kb on + strand at 260.361 kb on - strand at 260.361 kb on - strand at 260.533 kb on + strand at 260.533 kb on + strand at 260.533 kb on + strand at 260.609 kb on - strand at 260.609 kb on - strand at 260.611 kb on + strand at 260.611 kb on + strand at 260.611 kb on + strand at 260.611 kb on + strand at 260.611 kb on + strand at 260.611 kb on + strand at 260.612 kb on - strand at 260.643 kb on - strand at 260.692 kb on + strand at 260.692 kb on + strand at 260.693 kb on - strand at 260.786 kb on - strand, within EX31_RS01175 at 260.831 kb on + strand, within EX31_RS01175 at 260.831 kb on + strand, within EX31_RS01175 at 260.832 kb on - strand, within EX31_RS01175 at 260.832 kb on - strand, within EX31_RS01175 at 260.838 kb on + strand, within EX31_RS01175 at 261.077 kb on + strand, within EX31_RS01175 at 261.077 kb on + strand, within EX31_RS01175 at 261.078 kb on - strand, within EX31_RS01175 at 261.079 kb on + strand, within EX31_RS01175 at 261.079 kb on + strand, within EX31_RS01175 at 261.080 kb on - strand, within EX31_RS01175 at 261.153 kb on + strand, within EX31_RS01175 at 261.153 kb on + strand, within EX31_RS01175 at 261.154 kb on - strand, within EX31_RS01175 at 261.154 kb on - strand, within EX31_RS01175 at 261.278 kb on + strand, within EX31_RS01175 at 261.279 kb on - strand, within EX31_RS01175 at 261.279 kb on - strand, within EX31_RS01175 at 261.287 kb on + strand, within EX31_RS01175 at 261.288 kb on - strand, within EX31_RS01175 at 261.288 kb on - strand, within EX31_RS01175 at 261.320 kb on - strand, within EX31_RS01175 at 261.376 kb on + strand, within EX31_RS01175 at 261.422 kb on + strand, within EX31_RS01175 at 261.452 kb on + strand, within EX31_RS01175 at 261.452 kb on + strand, within EX31_RS01175 at 261.452 kb on + strand, within EX31_RS01175 at 261.452 kb on + strand, within EX31_RS01175 at 261.452 kb on + strand, within EX31_RS01175 at 261.452 kb on + strand, within EX31_RS01175 at 261.452 kb on + strand, within EX31_RS01175 at 261.452 kb on + strand, within EX31_RS01175 at 261.453 kb on - strand, within EX31_RS01175 at 261.453 kb on - strand, within EX31_RS01175 at 261.453 kb on - strand, within EX31_RS01175 at 261.453 kb on - strand, within EX31_RS01175 at 261.453 kb on - strand, within EX31_RS01175 at 261.454 kb on + strand, within EX31_RS01175 at 261.454 kb on + strand, within EX31_RS01175 at 261.454 kb on + strand, within EX31_RS01175 at 261.454 kb on + strand, within EX31_RS01175 at 261.454 kb on + strand, within EX31_RS01175 at 261.454 kb on + strand, within EX31_RS01175 at 261.454 kb on + strand, within EX31_RS01175 at 261.454 kb on + strand, within EX31_RS01175 at 261.454 kb on + strand, within EX31_RS01175 at 261.454 kb on + strand, within EX31_RS01175 at 261.454 kb on + strand, within EX31_RS01175 at 261.454 kb on + strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.455 kb on - strand, within EX31_RS01175 at 261.509 kb on - strand at 261.573 kb on - strand at 261.573 kb on - strand at 261.573 kb on - strand at 261.573 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 256,990 + +1.0 256,990 + +1.9 256,990 + -0.9 256,990 + +0.3 256,991 - -0.3 257,077 - -0.2 257,085 + -1.6 257,085 + +0.3 257,090 - -0.1 257,100 - -0.3 257,100 - -1.2 257,114 + -1.4 257,115 - -2.1 257,115 - +0.6 257,115 - -0.7 257,119 - +0.6 257,119 - +1.2 257,119 - +1.4 257,220 - +0.0 257,249 - +1.9 257,250 + -0.5 257,523 - -1.5 257,551 + -0.1 257,552 - -0.2 257,753 + -0.2 257,809 + -1.0 257,835 + -1.9 257,836 - +0.3 257,948 + +0.2 257,949 - +0.9 258,011 - -0.5 258,335 + EX31_RS01170 0.19 +0.1 258,336 - EX31_RS01170 0.19 -0.9 258,336 - EX31_RS01170 0.19 -0.1 258,462 - EX31_RS01170 0.23 -0.1 258,545 + EX31_RS01170 0.26 +0.8 258,545 + EX31_RS01170 0.26 -0.5 258,545 + EX31_RS01170 0.26 -1.4 258,659 + EX31_RS01170 0.30 -1.2 258,659 + EX31_RS01170 0.30 -0.1 258,659 + EX31_RS01170 0.30 -0.1 258,660 - EX31_RS01170 0.30 -1.0 258,725 - EX31_RS01170 0.33 -0.7 258,758 + EX31_RS01170 0.34 -1.6 258,758 + EX31_RS01170 0.34 -1.0 258,758 + EX31_RS01170 0.34 +0.1 259,118 - EX31_RS01170 0.47 -0.1 259,256 + EX31_RS01170 0.52 -0.8 259,455 - EX31_RS01170 0.59 +1.1 259,492 + EX31_RS01170 0.60 +0.7 259,672 + EX31_RS01170 0.67 +0.3 259,681 + EX31_RS01170 0.67 -2.7 259,681 + EX31_RS01170 0.67 -1.6 259,681 + EX31_RS01170 0.67 +0.3 259,682 - EX31_RS01170 0.67 +1.4 259,685 - EX31_RS01170 0.67 -0.5 259,851 - EX31_RS01170 0.73 -1.2 259,994 + EX31_RS01170 0.79 -0.8 259,995 - EX31_RS01170 0.79 +0.1 260,127 - EX31_RS01170 0.83 +0.8 260,161 + EX31_RS01170 0.85 -0.1 260,177 + EX31_RS01170 0.85 +0.7 260,177 + EX31_RS01170 0.85 +1.9 260,178 - EX31_RS01170 0.85 +1.6 260,276 + EX31_RS01170 0.89 -0.5 260,276 + EX31_RS01170 0.89 -0.6 260,277 - EX31_RS01170 0.89 +0.6 260,360 + +0.6 260,360 + +0.9 260,360 + +0.3 260,361 - -0.9 260,361 - -0.4 260,533 + -1.2 260,533 + -0.4 260,533 + -1.8 260,609 - -0.2 260,609 - -1.7 260,611 + +0.1 260,611 + -2.0 260,611 + +0.3 260,611 + +1.2 260,611 + +0.7 260,611 + +0.0 260,612 - +0.6 260,643 - -0.8 260,692 + +1.9 260,692 + +0.7 260,693 - +1.8 260,786 - EX31_RS01175 0.18 -0.1 260,831 + EX31_RS01175 0.22 +0.6 260,831 + EX31_RS01175 0.22 +1.5 260,832 - EX31_RS01175 0.23 -1.2 260,832 - EX31_RS01175 0.23 -0.9 260,838 + EX31_RS01175 0.23 +0.9 261,077 + EX31_RS01175 0.48 +0.7 261,077 + EX31_RS01175 0.48 +1.9 261,078 - EX31_RS01175 0.48 +1.9 261,079 + EX31_RS01175 0.48 -0.5 261,079 + EX31_RS01175 0.48 -0.5 261,080 - EX31_RS01175 0.48 -1.0 261,153 + EX31_RS01175 0.56 +2.3 261,153 + EX31_RS01175 0.56 -0.7 261,154 - EX31_RS01175 0.56 -1.7 261,154 - EX31_RS01175 0.56 +0.3 261,278 + EX31_RS01175 0.69 -1.0 261,279 - EX31_RS01175 0.69 -0.9 261,279 - EX31_RS01175 0.69 -1.5 261,287 + EX31_RS01175 0.70 +1.6 261,288 - EX31_RS01175 0.70 -2.7 261,288 - EX31_RS01175 0.70 -0.4 261,320 - EX31_RS01175 0.73 -1.4 261,376 + EX31_RS01175 0.79 +0.3 261,422 + EX31_RS01175 0.84 -1.2 261,452 + EX31_RS01175 0.87 +0.4 261,452 + EX31_RS01175 0.87 +0.2 261,452 + EX31_RS01175 0.87 +1.1 261,452 + EX31_RS01175 0.87 -0.7 261,452 + EX31_RS01175 0.87 -0.8 261,452 + EX31_RS01175 0.87 -0.2 261,452 + EX31_RS01175 0.87 +0.3 261,452 + EX31_RS01175 0.87 +0.6 261,453 - EX31_RS01175 0.87 +2.3 261,453 - EX31_RS01175 0.87 -1.2 261,453 - EX31_RS01175 0.87 -0.2 261,453 - EX31_RS01175 0.87 -0.3 261,453 - EX31_RS01175 0.87 +0.1 261,454 + EX31_RS01175 0.87 +1.4 261,454 + EX31_RS01175 0.87 +1.2 261,454 + EX31_RS01175 0.87 -0.1 261,454 + EX31_RS01175 0.87 -0.1 261,454 + EX31_RS01175 0.87 -0.2 261,454 + EX31_RS01175 0.87 -0.7 261,454 + EX31_RS01175 0.87 -0.9 261,454 + EX31_RS01175 0.87 +1.4 261,454 + EX31_RS01175 0.87 +0.5 261,454 + EX31_RS01175 0.87 -0.4 261,454 + EX31_RS01175 0.87 -1.7 261,454 + EX31_RS01175 0.87 +0.3 261,455 - EX31_RS01175 0.87 +2.3 261,455 - EX31_RS01175 0.87 -1.1 261,455 - EX31_RS01175 0.87 +1.3 261,455 - EX31_RS01175 0.87 +0.5 261,455 - EX31_RS01175 0.87 +1.3 261,455 - EX31_RS01175 0.87 +0.5 261,455 - EX31_RS01175 0.87 +1.9 261,455 - EX31_RS01175 0.87 -0.1 261,455 - EX31_RS01175 0.87 +1.6 261,455 - EX31_RS01175 0.87 -0.2 261,455 - EX31_RS01175 0.87 +1.5 261,455 - EX31_RS01175 0.87 -0.1 261,455 - EX31_RS01175 0.87 +0.2 261,455 - EX31_RS01175 0.87 -0.7 261,455 - EX31_RS01175 0.87 -0.4 261,455 - EX31_RS01175 0.87 +0.3 261,455 - EX31_RS01175 0.87 -0.5 261,455 - EX31_RS01175 0.87 +3.2 261,509 - +0.3 261,573 - +0.1 261,573 - +1.4 261,573 - -0.4 261,573 - -0.6
Or see this region's nucleotide sequence