Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00235 and EX31_RS00240 are separated by 68 nucleotides EX31_RS00240 and EX31_RS00245 are separated by 401 nucleotides
EX31_RS00235: EX31_RS00235 - sugar ABC transporter ATP-binding protein, at 47,733 to 49,247
_RS00235
EX31_RS00240: EX31_RS00240 - rhamnose ABC transporter substrate-binding protein, at 49,316 to 50,308
_RS00240
EX31_RS00245: EX31_RS00245 - HTH-type transcriptional activator RhaS, at 50,710 to 51,534
_RS00245
Position (kb)
49
50
51 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 48.337 kb on - strand, within EX31_RS00235 at 48.520 kb on - strand, within EX31_RS00235 at 48.577 kb on + strand, within EX31_RS00235 at 48.577 kb on + strand, within EX31_RS00235 at 48.577 kb on + strand, within EX31_RS00235 at 48.577 kb on + strand, within EX31_RS00235 at 48.578 kb on - strand, within EX31_RS00235 at 48.578 kb on - strand, within EX31_RS00235 at 48.578 kb on - strand, within EX31_RS00235 at 48.578 kb on - strand, within EX31_RS00235 at 48.578 kb on - strand, within EX31_RS00235 at 48.601 kb on + strand, within EX31_RS00235 at 48.601 kb on + strand, within EX31_RS00235 at 48.601 kb on + strand, within EX31_RS00235 at 48.601 kb on + strand, within EX31_RS00235 at 48.602 kb on - strand, within EX31_RS00235 at 48.602 kb on - strand, within EX31_RS00235 at 48.602 kb on - strand, within EX31_RS00235 at 48.602 kb on - strand, within EX31_RS00235 at 48.605 kb on - strand, within EX31_RS00235 at 48.807 kb on + strand, within EX31_RS00235 at 48.808 kb on - strand, within EX31_RS00235 at 48.808 kb on - strand, within EX31_RS00235 at 48.808 kb on - strand, within EX31_RS00235 at 48.840 kb on + strand, within EX31_RS00235 at 48.841 kb on - strand, within EX31_RS00235 at 48.841 kb on - strand, within EX31_RS00235 at 48.938 kb on + strand, within EX31_RS00235 at 49.046 kb on + strand, within EX31_RS00235 at 49.046 kb on + strand, within EX31_RS00235 at 49.046 kb on + strand, within EX31_RS00235 at 49.047 kb on - strand, within EX31_RS00235 at 49.047 kb on - strand, within EX31_RS00235 at 49.140 kb on + strand at 49.173 kb on - strand at 49.176 kb on + strand at 49.177 kb on - strand at 49.272 kb on - strand at 49.272 kb on - strand at 49.272 kb on - strand at 49.272 kb on - strand at 49.336 kb on + strand at 49.473 kb on + strand, within EX31_RS00240 at 49.473 kb on + strand, within EX31_RS00240 at 49.473 kb on + strand, within EX31_RS00240 at 49.474 kb on - strand, within EX31_RS00240 at 49.474 kb on - strand, within EX31_RS00240 at 49.474 kb on - strand, within EX31_RS00240 at 49.485 kb on + strand, within EX31_RS00240 at 49.581 kb on + strand, within EX31_RS00240 at 49.581 kb on + strand, within EX31_RS00240 at 49.581 kb on + strand, within EX31_RS00240 at 49.582 kb on - strand, within EX31_RS00240 at 49.582 kb on - strand, within EX31_RS00240 at 49.582 kb on - strand, within EX31_RS00240 at 49.582 kb on - strand, within EX31_RS00240 at 49.582 kb on - strand, within EX31_RS00240 at 49.582 kb on - strand, within EX31_RS00240 at 49.582 kb on - strand, within EX31_RS00240 at 49.582 kb on - strand, within EX31_RS00240 at 49.675 kb on - strand, within EX31_RS00240 at 49.839 kb on - strand, within EX31_RS00240 at 49.839 kb on - strand, within EX31_RS00240 at 49.927 kb on + strand, within EX31_RS00240 at 49.927 kb on + strand, within EX31_RS00240 at 49.928 kb on - strand, within EX31_RS00240 at 49.981 kb on + strand, within EX31_RS00240 at 49.982 kb on - strand, within EX31_RS00240 at 50.090 kb on + strand, within EX31_RS00240 at 50.091 kb on - strand, within EX31_RS00240 at 50.128 kb on - strand, within EX31_RS00240 at 50.128 kb on - strand, within EX31_RS00240 at 50.156 kb on - strand, within EX31_RS00240 at 50.201 kb on - strand, within EX31_RS00240 at 50.292 kb on - strand at 50.301 kb on - strand at 50.315 kb on + strand at 50.324 kb on + strand at 50.324 kb on + strand at 50.387 kb on + strand at 50.387 kb on + strand at 50.387 kb on + strand at 50.440 kb on - strand at 50.557 kb on + strand at 50.564 kb on + strand at 50.564 kb on + strand at 50.675 kb on + strand at 50.675 kb on + strand at 50.680 kb on + strand at 50.680 kb on + strand at 50.683 kb on + strand at 50.689 kb on - strand at 50.727 kb on + strand at 50.968 kb on + strand, within EX31_RS00245 at 51.096 kb on + strand, within EX31_RS00245 at 51.096 kb on + strand, within EX31_RS00245 at 51.155 kb on + strand, within EX31_RS00245 at 51.156 kb on - strand, within EX31_RS00245 at 51.156 kb on - strand, within EX31_RS00245 at 51.158 kb on - strand, within EX31_RS00245 at 51.162 kb on + strand, within EX31_RS00245 at 51.173 kb on + strand, within EX31_RS00245 at 51.173 kb on + strand, within EX31_RS00245 at 51.174 kb on - strand, within EX31_RS00245 at 51.176 kb on + strand, within EX31_RS00245 at 51.262 kb on + strand, within EX31_RS00245 at 51.263 kb on - strand, within EX31_RS00245 at 51.263 kb on - strand, within EX31_RS00245
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 48,337 - EX31_RS00235 0.40 -0.6 48,520 - EX31_RS00235 0.52 +0.2 48,577 + EX31_RS00235 0.56 +1.3 48,577 + EX31_RS00235 0.56 -2.2 48,577 + EX31_RS00235 0.56 -0.4 48,577 + EX31_RS00235 0.56 -0.2 48,578 - EX31_RS00235 0.56 +0.0 48,578 - EX31_RS00235 0.56 -1.7 48,578 - EX31_RS00235 0.56 -0.3 48,578 - EX31_RS00235 0.56 -0.2 48,578 - EX31_RS00235 0.56 -0.4 48,601 + EX31_RS00235 0.57 +0.9 48,601 + EX31_RS00235 0.57 -0.5 48,601 + EX31_RS00235 0.57 +0.6 48,601 + EX31_RS00235 0.57 -1.2 48,602 - EX31_RS00235 0.57 +0.8 48,602 - EX31_RS00235 0.57 +0.9 48,602 - EX31_RS00235 0.57 -0.4 48,602 - EX31_RS00235 0.57 +2.1 48,605 - EX31_RS00235 0.58 +0.4 48,807 + EX31_RS00235 0.71 -1.2 48,808 - EX31_RS00235 0.71 -1.4 48,808 - EX31_RS00235 0.71 -1.4 48,808 - EX31_RS00235 0.71 +0.1 48,840 + EX31_RS00235 0.73 -0.1 48,841 - EX31_RS00235 0.73 +0.6 48,841 - EX31_RS00235 0.73 +0.9 48,938 + EX31_RS00235 0.80 +0.3 49,046 + EX31_RS00235 0.87 -0.2 49,046 + EX31_RS00235 0.87 +1.7 49,046 + EX31_RS00235 0.87 -0.5 49,047 - EX31_RS00235 0.87 +0.1 49,047 - EX31_RS00235 0.87 +1.7 49,140 + +0.3 49,173 - -2.2 49,176 + +0.0 49,177 - +2.5 49,272 - +0.5 49,272 - +1.4 49,272 - -0.1 49,272 - -0.6 49,336 + -0.2 49,473 + EX31_RS00240 0.16 -0.1 49,473 + EX31_RS00240 0.16 +0.4 49,473 + EX31_RS00240 0.16 -1.2 49,474 - EX31_RS00240 0.16 -0.4 49,474 - EX31_RS00240 0.16 +0.1 49,474 - EX31_RS00240 0.16 -0.7 49,485 + EX31_RS00240 0.17 +0.1 49,581 + EX31_RS00240 0.27 -0.3 49,581 + EX31_RS00240 0.27 -0.5 49,581 + EX31_RS00240 0.27 +0.6 49,582 - EX31_RS00240 0.27 -0.9 49,582 - EX31_RS00240 0.27 -0.5 49,582 - EX31_RS00240 0.27 -1.9 49,582 - EX31_RS00240 0.27 -0.4 49,582 - EX31_RS00240 0.27 +1.9 49,582 - EX31_RS00240 0.27 +0.3 49,582 - EX31_RS00240 0.27 -1.8 49,582 - EX31_RS00240 0.27 -2.4 49,675 - EX31_RS00240 0.36 +0.8 49,839 - EX31_RS00240 0.53 +1.3 49,839 - EX31_RS00240 0.53 +1.3 49,927 + EX31_RS00240 0.62 -1.5 49,927 + EX31_RS00240 0.62 +0.2 49,928 - EX31_RS00240 0.62 -0.1 49,981 + EX31_RS00240 0.67 -1.1 49,982 - EX31_RS00240 0.67 -0.4 50,090 + EX31_RS00240 0.78 -0.4 50,091 - EX31_RS00240 0.78 -2.2 50,128 - EX31_RS00240 0.82 +0.2 50,128 - EX31_RS00240 0.82 +0.3 50,156 - EX31_RS00240 0.85 -1.5 50,201 - EX31_RS00240 0.89 +0.3 50,292 - -0.8 50,301 - +0.3 50,315 + +2.2 50,324 + -1.7 50,324 + +0.0 50,387 + +1.6 50,387 + +0.3 50,387 + +0.1 50,440 - +1.0 50,557 + -0.4 50,564 + +0.3 50,564 + -0.3 50,675 + -0.5 50,675 + -0.9 50,680 + -0.2 50,680 + +1.1 50,683 + -1.1 50,689 - -0.1 50,727 + +0.0 50,968 + EX31_RS00245 0.31 -0.7 51,096 + EX31_RS00245 0.47 -0.1 51,096 + EX31_RS00245 0.47 +0.1 51,155 + EX31_RS00245 0.54 -1.2 51,156 - EX31_RS00245 0.54 -0.4 51,156 - EX31_RS00245 0.54 +0.3 51,158 - EX31_RS00245 0.54 -1.3 51,162 + EX31_RS00245 0.55 +0.9 51,173 + EX31_RS00245 0.56 -0.1 51,173 + EX31_RS00245 0.56 -2.2 51,174 - EX31_RS00245 0.56 -0.4 51,176 + EX31_RS00245 0.56 +0.3 51,262 + EX31_RS00245 0.67 -0.8 51,263 - EX31_RS00245 0.67 +1.3 51,263 - EX31_RS00245 0.67 +1.2
Or see this region's nucleotide sequence