Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00070 and EX31_RS25875 are separated by 565 nucleotides EX31_RS25875 and EX31_RS00075 are separated by 600 nucleotides EX31_RS00075 and EX31_RS00080 overlap by 4 nucleotides
EX31_RS00070: EX31_RS00070 - replication initiation protein, at 12,756 to 13,742
_RS00070
EX31_RS25875: EX31_RS25875 - hypothetical protein, at 14,308 to 14,517
_RS25875
EX31_RS00075: EX31_RS00075 - chromosome partitioning protein ParB, at 15,118 to 15,327
_RS00075
EX31_RS00080: EX31_RS00080 - AAA family ATPase, at 15,324 to 15,950
_RS00080
Position (kb) 14
15 Strain fitness (log2 ratio) -2
-1
0
1
2 at 13.757 kb on - strand at 13.757 kb on - strand at 13.818 kb on + strand at 13.886 kb on + strand at 13.920 kb on - strand at 13.922 kb on + strand at 13.922 kb on + strand at 13.923 kb on - strand at 13.923 kb on - strand at 13.923 kb on - strand at 13.963 kb on + strand at 13.964 kb on - strand at 13.964 kb on - strand at 13.964 kb on - strand at 14.061 kb on + strand at 14.062 kb on - strand at 14.156 kb on - strand at 14.156 kb on - strand at 14.158 kb on - strand at 14.192 kb on - strand at 14.222 kb on - strand at 14.230 kb on + strand at 14.230 kb on + strand at 14.304 kb on - strand at 14.304 kb on - strand at 14.304 kb on - strand at 14.347 kb on - strand, within EX31_RS25875 at 14.380 kb on - strand, within EX31_RS25875 at 14.413 kb on - strand, within EX31_RS25875 at 14.431 kb on + strand, within EX31_RS25875 at 14.474 kb on - strand, within EX31_RS25875 at 14.486 kb on + strand, within EX31_RS25875 at 14.486 kb on + strand, within EX31_RS25875 at 14.519 kb on + strand at 14.520 kb on - strand at 14.570 kb on + strand at 14.571 kb on - strand at 14.634 kb on + strand at 14.634 kb on + strand at 14.634 kb on + strand at 14.634 kb on + strand at 14.634 kb on + strand at 14.634 kb on + strand at 14.634 kb on + strand at 14.635 kb on - strand at 14.635 kb on - strand at 14.635 kb on - strand at 14.643 kb on + strand at 14.643 kb on + strand at 14.644 kb on - strand at 14.673 kb on - strand at 14.718 kb on + strand at 14.774 kb on - strand at 14.781 kb on - strand at 14.973 kb on + strand at 15.028 kb on + strand at 15.119 kb on + strand at 15.119 kb on + strand at 15.120 kb on - strand at 15.120 kb on - strand at 15.120 kb on - strand at 15.120 kb on - strand at 15.120 kb on - strand at 15.120 kb on - strand at 15.120 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 13,757 - +0.8 13,757 - +0.6 13,818 + +0.2 13,886 + +0.6 13,920 - -1.0 13,922 + -1.5 13,922 + +1.2 13,923 - -1.4 13,923 - -0.1 13,923 - -0.4 13,963 + +0.5 13,964 - -0.1 13,964 - -0.1 13,964 - -0.2 14,061 + -0.5 14,062 - +1.4 14,156 - +0.5 14,156 - +0.8 14,158 - +0.9 14,192 - +1.9 14,222 - -0.9 14,230 + -0.2 14,230 + -2.2 14,304 - +0.2 14,304 - -0.1 14,304 - +0.2 14,347 - EX31_RS25875 0.19 -1.2 14,380 - EX31_RS25875 0.34 -1.8 14,413 - EX31_RS25875 0.50 -0.5 14,431 + EX31_RS25875 0.59 +0.6 14,474 - EX31_RS25875 0.79 -1.1 14,486 + EX31_RS25875 0.85 -0.8 14,486 + EX31_RS25875 0.85 -1.7 14,519 + -0.6 14,520 - -0.1 14,570 + +0.6 14,571 - +0.6 14,634 + +1.0 14,634 + +1.1 14,634 + +1.2 14,634 + +0.9 14,634 + -0.9 14,634 + -0.2 14,634 + -1.1 14,635 - +0.1 14,635 - +0.8 14,635 - +0.5 14,643 + +1.6 14,643 + -0.4 14,644 - -1.0 14,673 - +0.1 14,718 + +0.1 14,774 - -0.1 14,781 - -0.0 14,973 + -0.5 15,028 + +0.2 15,119 + -0.4 15,119 + +0.6 15,120 - +0.5 15,120 - -0.4 15,120 - -1.5 15,120 - -0.6 15,120 - -0.8 15,120 - +0.3 15,120 - +0.2
Or see this region's nucleotide sequence