Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS19980 and EX31_RS19985 are separated by 4 nucleotides EX31_RS19985 and EX31_RS19990 overlap by 14 nucleotides EX31_RS19990 and EX31_RS19995 are separated by 32 nucleotides EX31_RS19995 and EX31_RS20000 are separated by 67 nucleotides EX31_RS20000 and EX31_RS20005 are separated by 188 nucleotides
EX31_RS19980: EX31_RS19980 - transcriptional regulator CsgD, at 626,536 to 627,186
_RS19980
EX31_RS19985: EX31_RS19985 - curli production assembly/transport protein CsgE, at 627,191 to 627,586
_RS19985
EX31_RS19990: EX31_RS19990 - curli production assembly/transport protein CsgF, at 627,573 to 628,037
_RS19990
EX31_RS19995: EX31_RS19995 - curli production assembly/transport protein CsgG, at 628,070 to 628,903
_RS19995
EX31_RS20000: EX31_RS20000 - nuclear transport factor 2 family protein, at 628,971 to 629,429
_RS20000
EX31_RS20005: EX31_RS20005 - DUF4153 domain-containing protein, at 629,618 to 631,423
_RS20005
Position (kb) 628
629 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 627.110 kb on - strand, within EX31_RS19980 at 627.197 kb on - strand at 627.197 kb on - strand at 627.204 kb on + strand at 627.235 kb on - strand, within EX31_RS19985 at 627.270 kb on + strand, within EX31_RS19985 at 627.270 kb on + strand, within EX31_RS19985 at 627.270 kb on + strand, within EX31_RS19985 at 627.270 kb on + strand, within EX31_RS19985 at 627.270 kb on + strand, within EX31_RS19985 at 627.271 kb on - strand, within EX31_RS19985 at 627.271 kb on - strand, within EX31_RS19985 at 627.271 kb on - strand, within EX31_RS19985 at 627.271 kb on - strand, within EX31_RS19985 at 627.427 kb on - strand, within EX31_RS19985 at 627.443 kb on + strand, within EX31_RS19985 at 627.478 kb on + strand, within EX31_RS19985 at 627.510 kb on + strand, within EX31_RS19985 at 627.510 kb on + strand, within EX31_RS19985 at 627.511 kb on - strand, within EX31_RS19985 at 627.522 kb on + strand, within EX31_RS19985 at 627.537 kb on + strand, within EX31_RS19985 at 627.585 kb on - strand at 627.599 kb on + strand at 627.600 kb on - strand at 627.600 kb on - strand at 627.600 kb on - strand at 627.617 kb on + strand at 627.617 kb on + strand at 627.618 kb on - strand at 627.738 kb on - strand, within EX31_RS19990 at 627.738 kb on - strand, within EX31_RS19990 at 627.780 kb on + strand, within EX31_RS19990 at 627.780 kb on + strand, within EX31_RS19990 at 627.780 kb on + strand, within EX31_RS19990 at 627.781 kb on - strand, within EX31_RS19990 at 627.781 kb on - strand, within EX31_RS19990 at 627.781 kb on - strand, within EX31_RS19990 at 627.782 kb on + strand, within EX31_RS19990 at 627.782 kb on + strand, within EX31_RS19990 at 627.798 kb on + strand, within EX31_RS19990 at 627.799 kb on - strand, within EX31_RS19990 at 628.002 kb on - strand at 628.079 kb on + strand at 628.079 kb on + strand at 628.079 kb on + strand at 628.079 kb on + strand at 628.080 kb on - strand at 628.080 kb on - strand at 628.080 kb on - strand at 628.080 kb on - strand at 628.080 kb on - strand at 628.083 kb on + strand at 628.083 kb on + strand at 628.083 kb on + strand at 628.083 kb on + strand at 628.084 kb on - strand at 628.084 kb on - strand at 628.084 kb on - strand at 628.084 kb on - strand at 628.086 kb on + strand at 628.087 kb on - strand at 628.155 kb on + strand, within EX31_RS19995 at 628.155 kb on + strand, within EX31_RS19995 at 628.155 kb on + strand, within EX31_RS19995 at 628.156 kb on - strand, within EX31_RS19995 at 628.175 kb on + strand, within EX31_RS19995 at 628.175 kb on + strand, within EX31_RS19995 at 628.175 kb on + strand, within EX31_RS19995 at 628.176 kb on - strand, within EX31_RS19995 at 628.176 kb on - strand, within EX31_RS19995 at 628.176 kb on - strand, within EX31_RS19995 at 628.176 kb on - strand, within EX31_RS19995 at 628.176 kb on - strand, within EX31_RS19995 at 628.178 kb on - strand, within EX31_RS19995 at 628.178 kb on - strand, within EX31_RS19995 at 628.178 kb on - strand, within EX31_RS19995 at 628.265 kb on + strand, within EX31_RS19995 at 628.277 kb on - strand, within EX31_RS19995 at 628.439 kb on - strand, within EX31_RS19995 at 628.464 kb on + strand, within EX31_RS19995 at 628.464 kb on + strand, within EX31_RS19995 at 628.465 kb on - strand, within EX31_RS19995 at 628.474 kb on + strand, within EX31_RS19995 at 628.475 kb on - strand, within EX31_RS19995 at 628.475 kb on - strand, within EX31_RS19995 at 628.486 kb on - strand, within EX31_RS19995 at 628.510 kb on + strand, within EX31_RS19995 at 628.511 kb on - strand, within EX31_RS19995 at 628.569 kb on - strand, within EX31_RS19995 at 628.585 kb on - strand, within EX31_RS19995 at 628.629 kb on + strand, within EX31_RS19995 at 628.638 kb on + strand, within EX31_RS19995 at 628.638 kb on + strand, within EX31_RS19995 at 628.639 kb on - strand, within EX31_RS19995 at 628.642 kb on + strand, within EX31_RS19995 at 628.642 kb on + strand, within EX31_RS19995 at 628.730 kb on + strand, within EX31_RS19995 at 628.731 kb on - strand, within EX31_RS19995 at 628.755 kb on + strand, within EX31_RS19995 at 628.755 kb on + strand, within EX31_RS19995 at 628.755 kb on + strand, within EX31_RS19995 at 628.755 kb on + strand, within EX31_RS19995 at 628.755 kb on + strand, within EX31_RS19995 at 628.755 kb on + strand, within EX31_RS19995 at 628.755 kb on + strand, within EX31_RS19995 at 628.756 kb on - strand, within EX31_RS19995 at 628.756 kb on - strand, within EX31_RS19995 at 628.756 kb on - strand, within EX31_RS19995 at 628.756 kb on - strand, within EX31_RS19995 at 628.756 kb on - strand, within EX31_RS19995 at 628.791 kb on - strand, within EX31_RS19995 at 628.817 kb on - strand, within EX31_RS19995 at 628.817 kb on - strand, within EX31_RS19995 at 628.817 kb on - strand, within EX31_RS19995 at 628.871 kb on + strand at 628.871 kb on + strand at 628.871 kb on + strand at 628.871 kb on + strand at 628.871 kb on + strand at 628.872 kb on - strand at 628.872 kb on - strand at 628.872 kb on - strand at 628.873 kb on + strand at 628.873 kb on + strand at 628.873 kb on + strand at 628.873 kb on + strand at 628.874 kb on - strand at 628.874 kb on - strand at 628.874 kb on - strand at 628.874 kb on - strand at 629.008 kb on + strand at 629.012 kb on + strand at 629.012 kb on + strand at 629.012 kb on + strand at 629.012 kb on + strand at 629.013 kb on - strand at 629.013 kb on - strand at 629.039 kb on + strand, within EX31_RS20000 at 629.056 kb on + strand, within EX31_RS20000 at 629.056 kb on + strand, within EX31_RS20000 at 629.056 kb on + strand, within EX31_RS20000 at 629.057 kb on - strand, within EX31_RS20000 at 629.057 kb on - strand, within EX31_RS20000 at 629.057 kb on - strand, within EX31_RS20000 at 629.225 kb on + strand, within EX31_RS20000 at 629.226 kb on - strand, within EX31_RS20000 at 629.226 kb on - strand, within EX31_RS20000 at 629.226 kb on - strand, within EX31_RS20000 at 629.400 kb on - strand at 629.460 kb on - strand at 629.492 kb on - strand at 629.492 kb on - strand at 629.597 kb on + strand at 629.597 kb on + strand at 629.597 kb on + strand at 629.598 kb on - strand at 629.598 kb on - strand at 629.717 kb on + strand at 629.717 kb on + strand at 629.717 kb on + strand at 629.717 kb on + strand at 629.717 kb on + strand at 629.720 kb on + strand at 629.721 kb on - strand at 629.721 kb on - strand at 629.721 kb on - strand at 629.721 kb on - strand at 629.750 kb on + strand at 629.751 kb on - strand at 629.751 kb on - strand at 629.751 kb on - strand at 629.767 kb on + strand at 629.767 kb on + strand at 629.770 kb on + strand at 629.770 kb on + strand at 629.770 kb on + strand at 629.770 kb on + strand at 629.771 kb on - strand at 629.771 kb on - strand at 629.771 kb on - strand at 629.771 kb on - strand at 629.771 kb on - strand at 629.786 kb on - strand at 629.794 kb on - strand at 629.823 kb on + strand, within EX31_RS20005 at 629.823 kb on + strand, within EX31_RS20005 at 629.824 kb on - strand, within EX31_RS20005 at 629.824 kb on - strand, within EX31_RS20005 at 629.826 kb on + strand, within EX31_RS20005 at 629.826 kb on + strand, within EX31_RS20005
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 627,110 - EX31_RS19980 0.88 +0.4 627,197 - +1.1 627,197 - +0.6 627,204 + +0.3 627,235 - EX31_RS19985 0.11 +0.1 627,270 + EX31_RS19985 0.20 +0.6 627,270 + EX31_RS19985 0.20 -0.4 627,270 + EX31_RS19985 0.20 -0.9 627,270 + EX31_RS19985 0.20 +0.1 627,270 + EX31_RS19985 0.20 -0.1 627,271 - EX31_RS19985 0.20 +1.7 627,271 - EX31_RS19985 0.20 +0.4 627,271 - EX31_RS19985 0.20 -0.9 627,271 - EX31_RS19985 0.20 -0.7 627,427 - EX31_RS19985 0.60 +0.7 627,443 + EX31_RS19985 0.64 -0.6 627,478 + EX31_RS19985 0.72 -0.1 627,510 + EX31_RS19985 0.81 -0.7 627,510 + EX31_RS19985 0.81 -0.4 627,511 - EX31_RS19985 0.81 +0.6 627,522 + EX31_RS19985 0.84 -0.6 627,537 + EX31_RS19985 0.87 -0.0 627,585 - -0.2 627,599 + +0.0 627,600 - +0.3 627,600 - -2.1 627,600 - +0.8 627,617 + -0.6 627,617 + +0.6 627,618 - -0.4 627,738 - EX31_RS19990 0.35 +0.8 627,738 - EX31_RS19990 0.35 +1.5 627,780 + EX31_RS19990 0.45 +0.7 627,780 + EX31_RS19990 0.45 -0.4 627,780 + EX31_RS19990 0.45 +2.1 627,781 - EX31_RS19990 0.45 +0.6 627,781 - EX31_RS19990 0.45 +1.2 627,781 - EX31_RS19990 0.45 +0.3 627,782 + EX31_RS19990 0.45 +0.4 627,782 + EX31_RS19990 0.45 +0.4 627,798 + EX31_RS19990 0.48 +1.5 627,799 - EX31_RS19990 0.49 +0.8 628,002 - +0.3 628,079 + +0.0 628,079 + -0.8 628,079 + +0.0 628,079 + -0.7 628,080 - +1.3 628,080 - +1.0 628,080 - +0.4 628,080 - +0.2 628,080 - +0.2 628,083 + +0.3 628,083 + +0.4 628,083 + +2.4 628,083 + -1.3 628,084 - -0.7 628,084 - +0.3 628,084 - -1.6 628,084 - +1.1 628,086 + -0.4 628,087 - -0.1 628,155 + EX31_RS19995 0.10 +0.3 628,155 + EX31_RS19995 0.10 -1.0 628,155 + EX31_RS19995 0.10 +0.4 628,156 - EX31_RS19995 0.10 -0.5 628,175 + EX31_RS19995 0.13 +2.1 628,175 + EX31_RS19995 0.13 +0.2 628,175 + EX31_RS19995 0.13 -0.3 628,176 - EX31_RS19995 0.13 -0.1 628,176 - EX31_RS19995 0.13 -0.2 628,176 - EX31_RS19995 0.13 -1.0 628,176 - EX31_RS19995 0.13 +1.4 628,176 - EX31_RS19995 0.13 -1.0 628,178 - EX31_RS19995 0.13 +0.1 628,178 - EX31_RS19995 0.13 -1.6 628,178 - EX31_RS19995 0.13 -0.5 628,265 + EX31_RS19995 0.23 -0.2 628,277 - EX31_RS19995 0.25 -2.2 628,439 - EX31_RS19995 0.44 +0.6 628,464 + EX31_RS19995 0.47 -0.6 628,464 + EX31_RS19995 0.47 +2.1 628,465 - EX31_RS19995 0.47 -2.4 628,474 + EX31_RS19995 0.48 -0.5 628,475 - EX31_RS19995 0.49 +0.5 628,475 - EX31_RS19995 0.49 +0.3 628,486 - EX31_RS19995 0.50 -0.2 628,510 + EX31_RS19995 0.53 +0.8 628,511 - EX31_RS19995 0.53 +0.5 628,569 - EX31_RS19995 0.60 +0.1 628,585 - EX31_RS19995 0.62 -0.6 628,629 + EX31_RS19995 0.67 +1.0 628,638 + EX31_RS19995 0.68 +1.2 628,638 + EX31_RS19995 0.68 +1.1 628,639 - EX31_RS19995 0.68 -0.9 628,642 + EX31_RS19995 0.69 +0.6 628,642 + EX31_RS19995 0.69 +0.4 628,730 + EX31_RS19995 0.79 -1.1 628,731 - EX31_RS19995 0.79 +1.1 628,755 + EX31_RS19995 0.82 -1.0 628,755 + EX31_RS19995 0.82 -0.2 628,755 + EX31_RS19995 0.82 -0.3 628,755 + EX31_RS19995 0.82 -0.9 628,755 + EX31_RS19995 0.82 +0.5 628,755 + EX31_RS19995 0.82 -1.8 628,755 + EX31_RS19995 0.82 +0.4 628,756 - EX31_RS19995 0.82 +0.3 628,756 - EX31_RS19995 0.82 +1.6 628,756 - EX31_RS19995 0.82 +1.1 628,756 - EX31_RS19995 0.82 +0.1 628,756 - EX31_RS19995 0.82 +0.7 628,791 - EX31_RS19995 0.86 -0.2 628,817 - EX31_RS19995 0.90 -0.7 628,817 - EX31_RS19995 0.90 -0.4 628,817 - EX31_RS19995 0.90 +1.1 628,871 + +0.2 628,871 + -0.6 628,871 + +0.1 628,871 + +0.8 628,871 + +0.3 628,872 - -0.0 628,872 - +0.8 628,872 - -1.2 628,873 + +1.8 628,873 + +0.6 628,873 + +0.1 628,873 + -1.2 628,874 - +1.8 628,874 - +0.6 628,874 - -1.2 628,874 - +0.3 629,008 + -0.9 629,012 + -0.3 629,012 + +0.1 629,012 + +0.6 629,012 + +0.3 629,013 - -0.0 629,013 - -1.8 629,039 + EX31_RS20000 0.15 +0.1 629,056 + EX31_RS20000 0.19 -0.9 629,056 + EX31_RS20000 0.19 -0.8 629,056 + EX31_RS20000 0.19 +0.3 629,057 - EX31_RS20000 0.19 -3.2 629,057 - EX31_RS20000 0.19 +0.6 629,057 - EX31_RS20000 0.19 +1.0 629,225 + EX31_RS20000 0.55 -1.6 629,226 - EX31_RS20000 0.56 -0.2 629,226 - EX31_RS20000 0.56 +0.3 629,226 - EX31_RS20000 0.56 +1.0 629,400 - -0.4 629,460 - +1.0 629,492 - -1.0 629,492 - +0.6 629,597 + -0.2 629,597 + -0.7 629,597 + +1.1 629,598 - -1.7 629,598 - +1.5 629,717 + -0.8 629,717 + -0.0 629,717 + -1.1 629,717 + +0.3 629,717 + -0.4 629,720 + +1.0 629,721 - -0.2 629,721 - +0.2 629,721 - +0.3 629,721 - -0.5 629,750 + -2.3 629,751 - +0.3 629,751 - +0.1 629,751 - +1.2 629,767 + -0.3 629,767 + +0.0 629,770 + -1.3 629,770 + +1.2 629,770 + -0.1 629,770 + +0.1 629,771 - +1.2 629,771 - -0.2 629,771 - +2.0 629,771 - -1.7 629,771 - -0.5 629,786 - +0.6 629,794 - +2.3 629,823 + EX31_RS20005 0.11 -1.0 629,823 + EX31_RS20005 0.11 +0.2 629,824 - EX31_RS20005 0.11 -2.6 629,824 - EX31_RS20005 0.11 +0.2 629,826 + EX31_RS20005 0.12 +0.1 629,826 + EX31_RS20005 0.12 +0.6
Or see this region's nucleotide sequence