Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS17685 and EX31_RS17690 are separated by 299 nucleotides EX31_RS17690 and EX31_RS17695 are separated by 94 nucleotides EX31_RS17695 and EX31_RS17700 are separated by 111 nucleotides
EX31_RS17685: EX31_RS17685 - multidrug effflux MFS transporter, at 87,274 to 88,536
_RS17685
EX31_RS17690: EX31_RS17690 - levansucrase, at 88,836 to 90,083
_RS17690
EX31_RS17695: EX31_RS17695 - alpha/beta hydrolase, at 90,178 to 90,966
_RS17695
EX31_RS17700: EX31_RS17700 - efflux RND transporter permease subunit, at 91,078 to 94,152
_RS17700
Position (kb) 88
89
90
91 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 87.870 kb on + strand, within EX31_RS17685 at 87.871 kb on - strand, within EX31_RS17685 at 87.948 kb on + strand, within EX31_RS17685 at 87.948 kb on + strand, within EX31_RS17685 at 87.984 kb on + strand, within EX31_RS17685 at 87.984 kb on + strand, within EX31_RS17685 at 87.984 kb on + strand, within EX31_RS17685 at 87.984 kb on + strand, within EX31_RS17685 at 87.984 kb on + strand, within EX31_RS17685 at 87.985 kb on - strand, within EX31_RS17685 at 87.985 kb on - strand, within EX31_RS17685 at 87.985 kb on - strand, within EX31_RS17685 at 88.007 kb on - strand, within EX31_RS17685 at 88.007 kb on - strand, within EX31_RS17685 at 88.020 kb on + strand, within EX31_RS17685 at 88.025 kb on - strand, within EX31_RS17685 at 88.090 kb on + strand, within EX31_RS17685 at 88.090 kb on + strand, within EX31_RS17685 at 88.091 kb on - strand, within EX31_RS17685 at 88.091 kb on - strand, within EX31_RS17685 at 88.091 kb on - strand, within EX31_RS17685 at 88.091 kb on - strand, within EX31_RS17685 at 88.093 kb on - strand, within EX31_RS17685 at 88.093 kb on - strand, within EX31_RS17685 at 88.110 kb on + strand, within EX31_RS17685 at 88.111 kb on - strand, within EX31_RS17685 at 88.111 kb on - strand, within EX31_RS17685 at 88.113 kb on + strand, within EX31_RS17685 at 88.114 kb on - strand, within EX31_RS17685 at 88.130 kb on + strand, within EX31_RS17685 at 88.159 kb on + strand, within EX31_RS17685 at 88.160 kb on - strand, within EX31_RS17685 at 88.214 kb on - strand, within EX31_RS17685 at 88.266 kb on + strand, within EX31_RS17685 at 88.374 kb on + strand, within EX31_RS17685 at 88.374 kb on + strand, within EX31_RS17685 at 88.461 kb on - strand at 88.461 kb on - strand at 88.461 kb on - strand at 88.519 kb on - strand at 88.519 kb on - strand at 88.730 kb on - strand at 88.808 kb on - strand at 88.853 kb on + strand at 88.946 kb on + strand at 89.054 kb on + strand, within EX31_RS17690 at 89.063 kb on + strand, within EX31_RS17690 at 89.064 kb on - strand, within EX31_RS17690 at 89.074 kb on - strand, within EX31_RS17690 at 89.102 kb on + strand, within EX31_RS17690 at 89.275 kb on - strand, within EX31_RS17690 at 89.277 kb on + strand, within EX31_RS17690 at 89.277 kb on + strand, within EX31_RS17690 at 89.278 kb on - strand, within EX31_RS17690 at 89.278 kb on - strand, within EX31_RS17690 at 89.279 kb on + strand, within EX31_RS17690 at 89.279 kb on + strand, within EX31_RS17690 at 89.280 kb on - strand, within EX31_RS17690 at 89.372 kb on + strand, within EX31_RS17690 at 89.401 kb on - strand, within EX31_RS17690 at 89.401 kb on - strand, within EX31_RS17690 at 89.481 kb on + strand, within EX31_RS17690 at 89.481 kb on + strand, within EX31_RS17690 at 89.481 kb on + strand, within EX31_RS17690 at 89.482 kb on - strand, within EX31_RS17690 at 89.498 kb on + strand, within EX31_RS17690 at 89.603 kb on + strand, within EX31_RS17690 at 89.610 kb on - strand, within EX31_RS17690 at 89.728 kb on - strand, within EX31_RS17690 at 89.728 kb on - strand, within EX31_RS17690 at 89.728 kb on - strand, within EX31_RS17690 at 89.728 kb on - strand, within EX31_RS17690 at 89.730 kb on + strand, within EX31_RS17690 at 89.750 kb on + strand, within EX31_RS17690 at 89.750 kb on + strand, within EX31_RS17690 at 89.750 kb on + strand, within EX31_RS17690 at 89.750 kb on + strand, within EX31_RS17690 at 89.751 kb on - strand, within EX31_RS17690 at 89.751 kb on - strand, within EX31_RS17690 at 89.751 kb on - strand, within EX31_RS17690 at 89.760 kb on + strand, within EX31_RS17690 at 89.761 kb on - strand, within EX31_RS17690 at 89.761 kb on - strand, within EX31_RS17690 at 89.793 kb on + strand, within EX31_RS17690 at 89.861 kb on + strand, within EX31_RS17690 at 89.861 kb on + strand, within EX31_RS17690 at 89.889 kb on + strand, within EX31_RS17690 at 89.903 kb on - strand, within EX31_RS17690 at 89.930 kb on + strand, within EX31_RS17690 at 89.930 kb on + strand, within EX31_RS17690 at 89.931 kb on - strand, within EX31_RS17690 at 89.936 kb on + strand, within EX31_RS17690 at 89.967 kb on - strand at 89.967 kb on - strand at 89.987 kb on + strand at 90.025 kb on + strand at 90.026 kb on - strand at 90.026 kb on - strand at 90.048 kb on + strand at 90.048 kb on + strand at 90.048 kb on + strand at 90.048 kb on + strand at 90.048 kb on + strand at 90.048 kb on + strand at 90.048 kb on + strand at 90.048 kb on + strand at 90.048 kb on + strand at 90.048 kb on + strand at 90.048 kb on + strand at 90.049 kb on - strand at 90.049 kb on - strand at 90.050 kb on + strand at 90.072 kb on - strand at 90.072 kb on - strand at 90.077 kb on - strand at 90.081 kb on + strand at 90.182 kb on - strand at 90.205 kb on + strand at 90.205 kb on + strand at 90.206 kb on - strand at 90.206 kb on - strand at 90.302 kb on - strand, within EX31_RS17695 at 90.312 kb on - strand, within EX31_RS17695 at 90.335 kb on + strand, within EX31_RS17695 at 90.335 kb on + strand, within EX31_RS17695 at 90.336 kb on - strand, within EX31_RS17695 at 90.339 kb on + strand, within EX31_RS17695 at 90.340 kb on - strand, within EX31_RS17695 at 90.340 kb on - strand, within EX31_RS17695 at 90.395 kb on + strand, within EX31_RS17695 at 90.395 kb on + strand, within EX31_RS17695 at 90.395 kb on + strand, within EX31_RS17695 at 90.396 kb on - strand, within EX31_RS17695 at 90.396 kb on - strand, within EX31_RS17695 at 90.404 kb on + strand, within EX31_RS17695 at 90.704 kb on - strand, within EX31_RS17695 at 90.748 kb on + strand, within EX31_RS17695 at 90.749 kb on - strand, within EX31_RS17695 at 90.892 kb on - strand at 90.961 kb on - strand at 90.979 kb on + strand at 90.980 kb on - strand at 90.999 kb on + strand at 90.999 kb on + strand at 90.999 kb on + strand at 90.999 kb on + strand at 90.999 kb on + strand at 91.000 kb on - strand at 91.000 kb on - strand at 91.000 kb on - strand at 91.000 kb on - strand at 91.000 kb on - strand at 91.000 kb on - strand at 91.000 kb on - strand at 91.000 kb on - strand at 91.000 kb on - strand at 91.000 kb on - strand at 91.061 kb on + strand at 91.062 kb on - strand at 91.062 kb on - strand at 91.062 kb on - strand at 91.062 kb on - strand at 91.062 kb on - strand at 91.079 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 87,870 + EX31_RS17685 0.47 -0.3 87,871 - EX31_RS17685 0.47 -0.2 87,948 + EX31_RS17685 0.53 +1.0 87,948 + EX31_RS17685 0.53 -0.3 87,984 + EX31_RS17685 0.56 +1.3 87,984 + EX31_RS17685 0.56 +1.4 87,984 + EX31_RS17685 0.56 +1.0 87,984 + EX31_RS17685 0.56 +2.0 87,984 + EX31_RS17685 0.56 +0.2 87,985 - EX31_RS17685 0.56 +0.4 87,985 - EX31_RS17685 0.56 -0.1 87,985 - EX31_RS17685 0.56 +0.7 88,007 - EX31_RS17685 0.58 +1.0 88,007 - EX31_RS17685 0.58 -1.1 88,020 + EX31_RS17685 0.59 +1.7 88,025 - EX31_RS17685 0.59 +3.2 88,090 + EX31_RS17685 0.65 +1.2 88,090 + EX31_RS17685 0.65 -0.0 88,091 - EX31_RS17685 0.65 +1.3 88,091 - EX31_RS17685 0.65 +1.0 88,091 - EX31_RS17685 0.65 -0.6 88,091 - EX31_RS17685 0.65 +0.0 88,093 - EX31_RS17685 0.65 -1.0 88,093 - EX31_RS17685 0.65 +0.4 88,110 + EX31_RS17685 0.66 +3.2 88,111 - EX31_RS17685 0.66 +3.6 88,111 - EX31_RS17685 0.66 +0.9 88,113 + EX31_RS17685 0.66 +0.4 88,114 - EX31_RS17685 0.67 +0.0 88,130 + EX31_RS17685 0.68 +0.5 88,159 + EX31_RS17685 0.70 +0.1 88,160 - EX31_RS17685 0.70 +0.2 88,214 - EX31_RS17685 0.74 -1.1 88,266 + EX31_RS17685 0.79 -0.9 88,374 + EX31_RS17685 0.87 -0.8 88,374 + EX31_RS17685 0.87 -0.3 88,461 - -0.2 88,461 - +0.0 88,461 - +0.9 88,519 - +1.2 88,519 - +0.0 88,730 - +0.5 88,808 - +0.0 88,853 + -0.9 88,946 + -1.8 89,054 + EX31_RS17690 0.17 +1.3 89,063 + EX31_RS17690 0.18 +0.3 89,064 - EX31_RS17690 0.18 +1.6 89,074 - EX31_RS17690 0.19 -1.2 89,102 + EX31_RS17690 0.21 +0.7 89,275 - EX31_RS17690 0.35 +1.0 89,277 + EX31_RS17690 0.35 +1.0 89,277 + EX31_RS17690 0.35 -0.2 89,278 - EX31_RS17690 0.35 +0.9 89,278 - EX31_RS17690 0.35 +0.6 89,279 + EX31_RS17690 0.35 -1.1 89,279 + EX31_RS17690 0.35 +0.4 89,280 - EX31_RS17690 0.36 -0.3 89,372 + EX31_RS17690 0.43 -0.4 89,401 - EX31_RS17690 0.45 +1.3 89,401 - EX31_RS17690 0.45 -0.8 89,481 + EX31_RS17690 0.52 +0.3 89,481 + EX31_RS17690 0.52 +2.3 89,481 + EX31_RS17690 0.52 -1.7 89,482 - EX31_RS17690 0.52 +0.3 89,498 + EX31_RS17690 0.53 -1.0 89,603 + EX31_RS17690 0.61 +0.0 89,610 - EX31_RS17690 0.62 +3.0 89,728 - EX31_RS17690 0.71 +1.6 89,728 - EX31_RS17690 0.71 +1.4 89,728 - EX31_RS17690 0.71 -0.9 89,728 - EX31_RS17690 0.71 -1.2 89,730 + EX31_RS17690 0.72 +1.7 89,750 + EX31_RS17690 0.73 +1.0 89,750 + EX31_RS17690 0.73 -0.4 89,750 + EX31_RS17690 0.73 +0.8 89,750 + EX31_RS17690 0.73 +0.4 89,751 - EX31_RS17690 0.73 +0.4 89,751 - EX31_RS17690 0.73 -0.4 89,751 - EX31_RS17690 0.73 -1.5 89,760 + EX31_RS17690 0.74 +1.0 89,761 - EX31_RS17690 0.74 -1.4 89,761 - EX31_RS17690 0.74 +0.4 89,793 + EX31_RS17690 0.77 +3.0 89,861 + EX31_RS17690 0.82 +1.3 89,861 + EX31_RS17690 0.82 +0.3 89,889 + EX31_RS17690 0.84 +2.0 89,903 - EX31_RS17690 0.85 +1.3 89,930 + EX31_RS17690 0.88 +0.0 89,930 + EX31_RS17690 0.88 +0.3 89,931 - EX31_RS17690 0.88 +0.1 89,936 + EX31_RS17690 0.88 +0.3 89,967 - -0.2 89,967 - +0.3 89,987 + -0.3 90,025 + +0.5 90,026 - +1.3 90,026 - -0.7 90,048 + -0.1 90,048 + -0.6 90,048 + -0.3 90,048 + +0.5 90,048 + -0.9 90,048 + +1.3 90,048 + -1.2 90,048 + +0.1 90,048 + -0.8 90,048 + -0.2 90,048 + -0.7 90,049 - -0.7 90,049 - +0.8 90,050 + -0.4 90,072 - +0.0 90,072 - +1.2 90,077 - -0.3 90,081 + +2.0 90,182 - +0.5 90,205 + +0.7 90,205 + +2.0 90,206 - +1.0 90,206 - -0.3 90,302 - EX31_RS17695 0.16 +3.0 90,312 - EX31_RS17695 0.17 +1.0 90,335 + EX31_RS17695 0.20 +0.6 90,335 + EX31_RS17695 0.20 +0.4 90,336 - EX31_RS17695 0.20 +1.0 90,339 + EX31_RS17695 0.20 -0.4 90,340 - EX31_RS17695 0.21 -0.5 90,340 - EX31_RS17695 0.21 -0.7 90,395 + EX31_RS17695 0.28 +1.3 90,395 + EX31_RS17695 0.28 -1.0 90,395 + EX31_RS17695 0.28 +1.4 90,396 - EX31_RS17695 0.28 -0.2 90,396 - EX31_RS17695 0.28 +0.7 90,404 + EX31_RS17695 0.29 +1.0 90,704 - EX31_RS17695 0.67 -0.8 90,748 + EX31_RS17695 0.72 -0.3 90,749 - EX31_RS17695 0.72 +2.6 90,892 - -2.3 90,961 - +0.1 90,979 + -0.5 90,980 - -0.6 90,999 + +1.0 90,999 + +0.5 90,999 + +1.3 90,999 + -2.0 90,999 + +0.2 91,000 - +0.3 91,000 - +2.3 91,000 - +1.0 91,000 - +0.4 91,000 - -1.3 91,000 - +0.6 91,000 - -1.1 91,000 - +0.6 91,000 - +0.0 91,000 - +2.0 91,061 + +0.1 91,062 - +0.0 91,062 - +0.2 91,062 - +0.8 91,062 - +0.1 91,062 - +0.6 91,079 + -0.4
Or see this region's nucleotide sequence