Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12300 and EX31_RS12305 are separated by 77 nucleotides EX31_RS12305 and EX31_RS12310 are separated by 5 nucleotides EX31_RS12310 and EX31_RS12315 are separated by 52 nucleotides EX31_RS12315 and EX31_RS12320 are separated by 227 nucleotides
EX31_RS12300: EX31_RS12300 - NAD-dependent succinate-semialdehyde dehydrogenase, at 131,957 to 133,417
_RS12300
EX31_RS12305: EX31_RS12305 - ribonuclease, at 133,495 to 133,941
_RS12305
EX31_RS12310: EX31_RS12310 - hypothetical protein, at 133,947 to 134,240
_RS12310
EX31_RS12315: EX31_RS12315 - ribosome-associated protein, at 134,293 to 134,844
_RS12315
EX31_RS12320: EX31_RS12320 - metalloprotease PmbA, at 135,072 to 136,412
_RS12320
Position (kb) 133
134
135 Strain fitness (log2 ratio) -2
-1
0
1
2 at 132.965 kb on - strand, within EX31_RS12300 at 133.084 kb on + strand, within EX31_RS12300 at 133.085 kb on - strand, within EX31_RS12300 at 133.214 kb on + strand, within EX31_RS12300 at 133.214 kb on + strand, within EX31_RS12300 at 133.215 kb on - strand, within EX31_RS12300 at 133.215 kb on - strand, within EX31_RS12300 at 133.215 kb on - strand, within EX31_RS12300 at 133.215 kb on - strand, within EX31_RS12300 at 133.220 kb on + strand, within EX31_RS12300 at 133.220 kb on + strand, within EX31_RS12300 at 133.220 kb on + strand, within EX31_RS12300 at 133.221 kb on - strand, within EX31_RS12300 at 133.240 kb on + strand, within EX31_RS12300 at 133.240 kb on + strand, within EX31_RS12300 at 133.240 kb on + strand, within EX31_RS12300 at 133.240 kb on + strand, within EX31_RS12300 at 133.241 kb on - strand, within EX31_RS12300 at 133.261 kb on + strand, within EX31_RS12300 at 133.261 kb on + strand, within EX31_RS12300 at 133.262 kb on - strand, within EX31_RS12300 at 133.329 kb on + strand at 133.329 kb on + strand at 133.330 kb on - strand at 133.330 kb on - strand at 133.330 kb on - strand at 133.330 kb on - strand at 133.379 kb on + strand at 133.379 kb on + strand at 133.405 kb on - strand at 133.415 kb on + strand at 133.415 kb on + strand at 133.415 kb on + strand at 133.415 kb on + strand at 133.416 kb on - strand at 133.436 kb on - strand at 133.436 kb on - strand at 133.594 kb on + strand, within EX31_RS12305 at 133.594 kb on + strand, within EX31_RS12305 at 133.594 kb on + strand, within EX31_RS12305 at 133.594 kb on + strand, within EX31_RS12305 at 133.594 kb on + strand, within EX31_RS12305 at 133.594 kb on + strand, within EX31_RS12305 at 133.594 kb on + strand, within EX31_RS12305 at 133.594 kb on + strand, within EX31_RS12305 at 133.594 kb on + strand, within EX31_RS12305 at 133.594 kb on + strand, within EX31_RS12305 at 133.594 kb on + strand, within EX31_RS12305 at 133.594 kb on + strand, within EX31_RS12305 at 133.594 kb on + strand, within EX31_RS12305 at 133.594 kb on + strand, within EX31_RS12305 at 133.595 kb on - strand, within EX31_RS12305 at 133.595 kb on - strand, within EX31_RS12305 at 133.595 kb on - strand, within EX31_RS12305 at 133.595 kb on - strand, within EX31_RS12305 at 133.595 kb on - strand, within EX31_RS12305 at 133.595 kb on - strand, within EX31_RS12305 at 133.595 kb on - strand, within EX31_RS12305 at 133.595 kb on - strand, within EX31_RS12305 at 133.595 kb on - strand, within EX31_RS12305 at 133.595 kb on - strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.596 kb on + strand, within EX31_RS12305 at 133.597 kb on - strand, within EX31_RS12305 at 133.597 kb on - strand, within EX31_RS12305 at 133.597 kb on - strand, within EX31_RS12305 at 133.597 kb on - strand, within EX31_RS12305 at 133.597 kb on - strand, within EX31_RS12305 at 133.597 kb on - strand, within EX31_RS12305 at 133.597 kb on - strand, within EX31_RS12305 at 133.597 kb on - strand, within EX31_RS12305 at 133.610 kb on + strand, within EX31_RS12305 at 133.610 kb on + strand, within EX31_RS12305 at 133.610 kb on + strand, within EX31_RS12305 at 133.611 kb on - strand, within EX31_RS12305 at 133.611 kb on - strand, within EX31_RS12305 at 133.611 kb on - strand, within EX31_RS12305 at 133.611 kb on - strand, within EX31_RS12305 at 133.611 kb on - strand, within EX31_RS12305 at 133.611 kb on - strand, within EX31_RS12305 at 133.645 kb on + strand, within EX31_RS12305 at 133.646 kb on - strand, within EX31_RS12305 at 133.649 kb on + strand, within EX31_RS12305 at 133.649 kb on + strand, within EX31_RS12305 at 133.662 kb on + strand, within EX31_RS12305 at 133.678 kb on + strand, within EX31_RS12305 at 133.678 kb on + strand, within EX31_RS12305 at 133.679 kb on - strand, within EX31_RS12305 at 133.733 kb on + strand, within EX31_RS12305 at 133.733 kb on + strand, within EX31_RS12305 at 133.734 kb on - strand, within EX31_RS12305 at 133.734 kb on - strand, within EX31_RS12305 at 133.807 kb on + strand, within EX31_RS12305 at 133.807 kb on + strand, within EX31_RS12305 at 133.808 kb on - strand, within EX31_RS12305 at 133.833 kb on + strand, within EX31_RS12305 at 133.834 kb on - strand, within EX31_RS12305 at 133.836 kb on + strand, within EX31_RS12305 at 133.836 kb on + strand, within EX31_RS12305 at 133.836 kb on + strand, within EX31_RS12305 at 133.836 kb on + strand, within EX31_RS12305 at 133.836 kb on + strand, within EX31_RS12305 at 133.836 kb on + strand, within EX31_RS12305 at 133.836 kb on + strand, within EX31_RS12305 at 133.836 kb on + strand, within EX31_RS12305 at 133.837 kb on - strand, within EX31_RS12305 at 133.837 kb on - strand, within EX31_RS12305 at 133.837 kb on - strand, within EX31_RS12305 at 133.837 kb on - strand, within EX31_RS12305 at 133.837 kb on - strand, within EX31_RS12305 at 133.837 kb on - strand, within EX31_RS12305 at 133.837 kb on - strand, within EX31_RS12305 at 133.837 kb on - strand, within EX31_RS12305 at 133.837 kb on - strand, within EX31_RS12305 at 133.840 kb on + strand, within EX31_RS12305 at 133.840 kb on + strand, within EX31_RS12305 at 133.840 kb on + strand, within EX31_RS12305 at 133.841 kb on - strand, within EX31_RS12305 at 133.897 kb on + strand at 133.897 kb on + strand at 133.897 kb on + strand at 133.897 kb on + strand at 133.897 kb on + strand at 133.897 kb on + strand at 133.897 kb on + strand at 133.897 kb on + strand at 133.897 kb on + strand at 133.897 kb on + strand at 133.897 kb on + strand at 133.897 kb on + strand at 133.898 kb on - strand at 133.898 kb on - strand at 133.905 kb on + strand at 133.952 kb on + strand at 134.101 kb on + strand, within EX31_RS12310 at 134.101 kb on + strand, within EX31_RS12310 at 134.101 kb on + strand, within EX31_RS12310 at 134.102 kb on - strand, within EX31_RS12310 at 134.112 kb on - strand, within EX31_RS12310 at 134.130 kb on - strand, within EX31_RS12310 at 134.130 kb on - strand, within EX31_RS12310 at 134.201 kb on + strand, within EX31_RS12310 at 134.201 kb on + strand, within EX31_RS12310 at 134.201 kb on + strand, within EX31_RS12310 at 134.231 kb on + strand at 134.270 kb on + strand at 134.270 kb on + strand at 134.271 kb on - strand at 134.271 kb on - strand at 134.271 kb on - strand at 134.271 kb on - strand at 134.324 kb on + strand at 134.325 kb on - strand at 134.325 kb on - strand at 134.426 kb on + strand, within EX31_RS12315 at 134.427 kb on - strand, within EX31_RS12315 at 134.427 kb on - strand, within EX31_RS12315 at 134.427 kb on - strand, within EX31_RS12315 at 134.497 kb on - strand, within EX31_RS12315 at 134.497 kb on - strand, within EX31_RS12315 at 134.587 kb on - strand, within EX31_RS12315 at 134.599 kb on + strand, within EX31_RS12315 at 134.600 kb on - strand, within EX31_RS12315 at 134.600 kb on - strand, within EX31_RS12315 at 134.638 kb on + strand, within EX31_RS12315 at 134.638 kb on + strand, within EX31_RS12315 at 134.749 kb on - strand, within EX31_RS12315 at 134.749 kb on - strand, within EX31_RS12315 at 134.749 kb on - strand, within EX31_RS12315 at 134.760 kb on + strand, within EX31_RS12315 at 134.760 kb on + strand, within EX31_RS12315 at 134.858 kb on + strand at 134.859 kb on - strand at 134.866 kb on + strand at 134.866 kb on + strand at 134.866 kb on + strand at 134.866 kb on + strand at 134.867 kb on - strand at 134.867 kb on - strand at 134.867 kb on - strand at 134.867 kb on - strand at 134.867 kb on - strand at 134.867 kb on - strand at 134.891 kb on + strand at 134.892 kb on - strand at 134.892 kb on - strand at 134.909 kb on - strand at 134.919 kb on - strand at 134.927 kb on + strand at 134.927 kb on + strand at 134.927 kb on + strand at 134.927 kb on + strand at 134.927 kb on + strand at 134.927 kb on + strand at 134.927 kb on + strand at 134.927 kb on + strand at 134.928 kb on - strand at 134.928 kb on - strand at 134.928 kb on - strand at 134.928 kb on - strand at 134.928 kb on - strand at 134.928 kb on - strand at 134.928 kb on - strand at 134.928 kb on - strand at 134.928 kb on - strand at 134.928 kb on - strand at 134.928 kb on - strand at 134.928 kb on - strand at 134.928 kb on - strand at 134.957 kb on + strand at 134.957 kb on + strand at 134.958 kb on - strand at 134.958 kb on - strand at 134.958 kb on - strand at 134.997 kb on + strand at 135.011 kb on + strand at 135.011 kb on + strand at 135.023 kb on + strand at 135.024 kb on - strand at 135.024 kb on - strand at 135.024 kb on - strand at 135.025 kb on + strand at 135.025 kb on + strand at 135.025 kb on + strand at 135.025 kb on + strand at 135.026 kb on - strand at 135.026 kb on - strand at 135.033 kb on + strand at 135.033 kb on + strand at 135.034 kb on - strand at 135.048 kb on + strand at 135.048 kb on + strand at 135.048 kb on + strand at 135.049 kb on - strand at 135.049 kb on - strand at 135.049 kb on - strand at 135.049 kb on - strand at 135.049 kb on - strand at 135.079 kb on + strand at 135.079 kb on + strand at 135.079 kb on + strand at 135.080 kb on - strand at 135.080 kb on - strand at 135.080 kb on - strand at 135.091 kb on + strand at 135.091 kb on + strand at 135.092 kb on - strand at 135.104 kb on + strand at 135.104 kb on + strand at 135.104 kb on + strand at 135.104 kb on + strand at 135.104 kb on + strand at 135.105 kb on - strand at 135.105 kb on - strand at 135.105 kb on - strand at 135.124 kb on + strand at 135.124 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 132,965 - EX31_RS12300 0.69 -0.1 133,084 + EX31_RS12300 0.77 +0.1 133,085 - EX31_RS12300 0.77 +0.4 133,214 + EX31_RS12300 0.86 -0.2 133,214 + EX31_RS12300 0.86 -0.2 133,215 - EX31_RS12300 0.86 -0.0 133,215 - EX31_RS12300 0.86 +1.0 133,215 - EX31_RS12300 0.86 +0.7 133,215 - EX31_RS12300 0.86 -1.0 133,220 + EX31_RS12300 0.86 -0.2 133,220 + EX31_RS12300 0.86 -0.4 133,220 + EX31_RS12300 0.86 +0.4 133,221 - EX31_RS12300 0.87 +0.4 133,240 + EX31_RS12300 0.88 +0.5 133,240 + EX31_RS12300 0.88 +0.6 133,240 + EX31_RS12300 0.88 +0.6 133,240 + EX31_RS12300 0.88 +0.6 133,241 - EX31_RS12300 0.88 +0.1 133,261 + EX31_RS12300 0.89 +0.4 133,261 + EX31_RS12300 0.89 +0.2 133,262 - EX31_RS12300 0.89 +0.4 133,329 + +0.1 133,329 + -0.2 133,330 - +0.8 133,330 - -0.9 133,330 - -0.8 133,330 - -0.1 133,379 + -1.4 133,379 + -1.2 133,405 - +0.4 133,415 + +0.4 133,415 + -1.0 133,415 + +0.8 133,415 + -1.5 133,416 - -0.4 133,436 - -1.2 133,436 - -0.5 133,594 + EX31_RS12305 0.22 +0.5 133,594 + EX31_RS12305 0.22 -0.7 133,594 + EX31_RS12305 0.22 +0.4 133,594 + EX31_RS12305 0.22 +0.1 133,594 + EX31_RS12305 0.22 -0.8 133,594 + EX31_RS12305 0.22 -0.4 133,594 + EX31_RS12305 0.22 +1.1 133,594 + EX31_RS12305 0.22 -1.8 133,594 + EX31_RS12305 0.22 +0.1 133,594 + EX31_RS12305 0.22 +1.0 133,594 + EX31_RS12305 0.22 +0.8 133,594 + EX31_RS12305 0.22 -1.2 133,594 + EX31_RS12305 0.22 +0.4 133,594 + EX31_RS12305 0.22 +0.1 133,595 - EX31_RS12305 0.22 +1.6 133,595 - EX31_RS12305 0.22 +0.2 133,595 - EX31_RS12305 0.22 +1.3 133,595 - EX31_RS12305 0.22 -0.1 133,595 - EX31_RS12305 0.22 +0.8 133,595 - EX31_RS12305 0.22 -0.4 133,595 - EX31_RS12305 0.22 -0.1 133,595 - EX31_RS12305 0.22 -0.3 133,595 - EX31_RS12305 0.22 +0.0 133,595 - EX31_RS12305 0.22 -0.4 133,596 + EX31_RS12305 0.23 -0.0 133,596 + EX31_RS12305 0.23 -0.9 133,596 + EX31_RS12305 0.23 +0.3 133,596 + EX31_RS12305 0.23 +0.8 133,596 + EX31_RS12305 0.23 -0.2 133,596 + EX31_RS12305 0.23 +1.3 133,596 + EX31_RS12305 0.23 -0.3 133,596 + EX31_RS12305 0.23 -1.1 133,596 + EX31_RS12305 0.23 -0.1 133,596 + EX31_RS12305 0.23 -1.1 133,596 + EX31_RS12305 0.23 +1.2 133,596 + EX31_RS12305 0.23 -0.1 133,596 + EX31_RS12305 0.23 -1.5 133,596 + EX31_RS12305 0.23 +0.7 133,596 + EX31_RS12305 0.23 -0.3 133,596 + EX31_RS12305 0.23 -0.0 133,597 - EX31_RS12305 0.23 +0.2 133,597 - EX31_RS12305 0.23 +0.1 133,597 - EX31_RS12305 0.23 +0.2 133,597 - EX31_RS12305 0.23 -0.0 133,597 - EX31_RS12305 0.23 +0.3 133,597 - EX31_RS12305 0.23 +0.1 133,597 - EX31_RS12305 0.23 +0.4 133,597 - EX31_RS12305 0.23 +0.5 133,610 + EX31_RS12305 0.26 +0.3 133,610 + EX31_RS12305 0.26 +0.5 133,610 + EX31_RS12305 0.26 -0.2 133,611 - EX31_RS12305 0.26 -0.4 133,611 - EX31_RS12305 0.26 -0.0 133,611 - EX31_RS12305 0.26 +1.5 133,611 - EX31_RS12305 0.26 +1.0 133,611 - EX31_RS12305 0.26 -0.0 133,611 - EX31_RS12305 0.26 +1.0 133,645 + EX31_RS12305 0.34 +0.1 133,646 - EX31_RS12305 0.34 +1.1 133,649 + EX31_RS12305 0.34 -1.0 133,649 + EX31_RS12305 0.34 +0.5 133,662 + EX31_RS12305 0.37 +0.1 133,678 + EX31_RS12305 0.41 -0.6 133,678 + EX31_RS12305 0.41 -0.4 133,679 - EX31_RS12305 0.41 -1.0 133,733 + EX31_RS12305 0.53 +0.2 133,733 + EX31_RS12305 0.53 +0.3 133,734 - EX31_RS12305 0.53 -0.6 133,734 - EX31_RS12305 0.53 -0.8 133,807 + EX31_RS12305 0.70 -2.2 133,807 + EX31_RS12305 0.70 -0.5 133,808 - EX31_RS12305 0.70 +0.3 133,833 + EX31_RS12305 0.76 +0.2 133,834 - EX31_RS12305 0.76 +0.1 133,836 + EX31_RS12305 0.76 +0.4 133,836 + EX31_RS12305 0.76 -0.2 133,836 + EX31_RS12305 0.76 +0.5 133,836 + EX31_RS12305 0.76 +0.5 133,836 + EX31_RS12305 0.76 +0.0 133,836 + EX31_RS12305 0.76 +0.7 133,836 + EX31_RS12305 0.76 +0.3 133,836 + EX31_RS12305 0.76 -0.4 133,837 - EX31_RS12305 0.77 +0.4 133,837 - EX31_RS12305 0.77 +1.5 133,837 - EX31_RS12305 0.77 +1.8 133,837 - EX31_RS12305 0.77 -1.4 133,837 - EX31_RS12305 0.77 +2.3 133,837 - EX31_RS12305 0.77 +0.8 133,837 - EX31_RS12305 0.77 -0.5 133,837 - EX31_RS12305 0.77 -0.6 133,837 - EX31_RS12305 0.77 -0.0 133,840 + EX31_RS12305 0.77 +0.5 133,840 + EX31_RS12305 0.77 +0.6 133,840 + EX31_RS12305 0.77 -0.7 133,841 - EX31_RS12305 0.77 +0.8 133,897 + -0.3 133,897 + +1.0 133,897 + -0.4 133,897 + +0.4 133,897 + -0.7 133,897 + +1.1 133,897 + +0.0 133,897 + -0.0 133,897 + -0.5 133,897 + +0.2 133,897 + -0.0 133,897 + +0.7 133,898 - +1.1 133,898 - +1.1 133,905 + -0.4 133,952 + +0.5 134,101 + EX31_RS12310 0.52 +0.1 134,101 + EX31_RS12310 0.52 +0.3 134,101 + EX31_RS12310 0.52 -1.2 134,102 - EX31_RS12310 0.53 +0.2 134,112 - EX31_RS12310 0.56 +1.4 134,130 - EX31_RS12310 0.62 +1.8 134,130 - EX31_RS12310 0.62 +1.6 134,201 + EX31_RS12310 0.86 +0.8 134,201 + EX31_RS12310 0.86 -0.5 134,201 + EX31_RS12310 0.86 -0.0 134,231 + -0.4 134,270 + -0.3 134,270 + -0.1 134,271 - -0.3 134,271 - -0.6 134,271 - -1.9 134,271 - -1.3 134,324 + -0.8 134,325 - +0.1 134,325 - -0.8 134,426 + EX31_RS12315 0.24 -0.8 134,427 - EX31_RS12315 0.24 -0.9 134,427 - EX31_RS12315 0.24 -1.1 134,427 - EX31_RS12315 0.24 -0.9 134,497 - EX31_RS12315 0.37 +0.5 134,497 - EX31_RS12315 0.37 +0.3 134,587 - EX31_RS12315 0.53 -1.5 134,599 + EX31_RS12315 0.55 -0.0 134,600 - EX31_RS12315 0.56 -1.0 134,600 - EX31_RS12315 0.56 -0.3 134,638 + EX31_RS12315 0.62 -0.0 134,638 + EX31_RS12315 0.62 -0.7 134,749 - EX31_RS12315 0.83 -0.9 134,749 - EX31_RS12315 0.83 +1.1 134,749 - EX31_RS12315 0.83 -0.3 134,760 + EX31_RS12315 0.85 -0.0 134,760 + EX31_RS12315 0.85 -0.7 134,858 + -0.0 134,859 - -0.1 134,866 + -0.2 134,866 + -0.1 134,866 + -0.6 134,866 + -0.1 134,867 - +0.4 134,867 - +0.0 134,867 - +1.3 134,867 - -0.4 134,867 - -0.3 134,867 - -0.1 134,891 + +0.2 134,892 - +1.8 134,892 - -0.1 134,909 - -0.4 134,919 - +0.2 134,927 + -0.2 134,927 + +0.2 134,927 + +0.7 134,927 + -0.3 134,927 + -0.3 134,927 + +0.2 134,927 + -0.6 134,927 + -0.6 134,928 - -0.4 134,928 - -0.3 134,928 - -0.3 134,928 - +0.9 134,928 - +0.3 134,928 - -0.7 134,928 - -0.4 134,928 - +0.0 134,928 - -0.6 134,928 - -0.2 134,928 - +0.5 134,928 - +0.5 134,928 - +0.1 134,957 + +0.5 134,957 + +0.4 134,958 - -0.5 134,958 - +0.1 134,958 - -1.4 134,997 + -1.9 135,011 + +0.0 135,011 + +1.0 135,023 + -0.2 135,024 - -0.3 135,024 - -1.2 135,024 - -0.2 135,025 + +0.6 135,025 + -1.4 135,025 + -1.5 135,025 + -0.9 135,026 - -0.4 135,026 - -0.3 135,033 + -1.1 135,033 + -0.4 135,034 - +0.3 135,048 + +1.4 135,048 + +0.0 135,048 + +1.3 135,049 - -0.2 135,049 - +1.0 135,049 - +0.1 135,049 - +0.5 135,049 - +0.2 135,079 + +0.2 135,079 + -0.6 135,079 + +0.0 135,080 - +0.5 135,080 - +1.6 135,080 - +0.2 135,091 + +0.3 135,091 + +0.5 135,092 - -0.5 135,104 + +0.4 135,104 + +0.1 135,104 + -1.8 135,104 + -0.7 135,104 + +1.4 135,105 - +0.9 135,105 - +0.1 135,105 - +2.3 135,124 + -0.4 135,124 + -0.1
Or see this region's nucleotide sequence