Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09545 and EX31_RS09550 overlap by 8 nucleotides EX31_RS09550 and EX31_RS09555 overlap by 1 nucleotides EX31_RS09555 and EX31_RS09560 are separated by 21 nucleotides EX31_RS09560 and EX31_RS09565 overlap by 4 nucleotides
EX31_RS09545: EX31_RS09545 - flagellar motor switch protein FliG, at 113,787 to 114,779
_RS09545
EX31_RS09550: EX31_RS09550 - flagellar assembly protein FliH, at 114,772 to 115,557
_RS09550
EX31_RS09555: EX31_RS09555 - flagellum-specific ATP synthase FliI, at 115,557 to 116,921
_RS09555
EX31_RS09560: EX31_RS09560 - flagella biosynthesis chaperone FliJ, at 116,943 to 117,389
_RS09560
EX31_RS09565: EX31_RS09565 - flagellar hook-length control protein FliK, at 117,386 to 118,774
_RS09565
Position (kb)
115
116
117 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 114.674 kb on + strand, within EX31_RS09545 at 114.674 kb on + strand, within EX31_RS09545 at 114.674 kb on + strand, within EX31_RS09545 at 114.674 kb on + strand, within EX31_RS09545 at 114.675 kb on - strand, within EX31_RS09545 at 114.675 kb on - strand, within EX31_RS09545 at 114.771 kb on + strand at 114.772 kb on - strand at 114.772 kb on - strand at 114.786 kb on + strand at 114.786 kb on + strand at 114.790 kb on - strand at 114.790 kb on - strand at 114.987 kb on - strand, within EX31_RS09550 at 115.170 kb on + strand, within EX31_RS09550 at 115.171 kb on - strand, within EX31_RS09550 at 115.320 kb on + strand, within EX31_RS09550 at 115.320 kb on + strand, within EX31_RS09550 at 115.694 kb on - strand, within EX31_RS09555 at 115.839 kb on + strand, within EX31_RS09555 at 115.839 kb on + strand, within EX31_RS09555 at 115.839 kb on + strand, within EX31_RS09555 at 115.839 kb on + strand, within EX31_RS09555 at 115.915 kb on + strand, within EX31_RS09555 at 115.915 kb on + strand, within EX31_RS09555 at 115.915 kb on + strand, within EX31_RS09555 at 115.915 kb on + strand, within EX31_RS09555 at 115.915 kb on + strand, within EX31_RS09555 at 116.287 kb on + strand, within EX31_RS09555 at 116.287 kb on + strand, within EX31_RS09555 at 116.287 kb on + strand, within EX31_RS09555 at 116.287 kb on + strand, within EX31_RS09555 at 116.288 kb on - strand, within EX31_RS09555 at 116.288 kb on - strand, within EX31_RS09555 at 116.291 kb on + strand, within EX31_RS09555 at 116.291 kb on + strand, within EX31_RS09555 at 116.291 kb on + strand, within EX31_RS09555 at 116.292 kb on - strand, within EX31_RS09555 at 116.292 kb on - strand, within EX31_RS09555 at 116.353 kb on + strand, within EX31_RS09555 at 116.443 kb on - strand, within EX31_RS09555 at 116.530 kb on - strand, within EX31_RS09555 at 116.530 kb on - strand, within EX31_RS09555 at 116.536 kb on + strand, within EX31_RS09555 at 116.536 kb on + strand, within EX31_RS09555 at 116.537 kb on - strand, within EX31_RS09555 at 116.690 kb on + strand, within EX31_RS09555 at 116.745 kb on + strand, within EX31_RS09555 at 116.746 kb on - strand, within EX31_RS09555 at 116.781 kb on + strand, within EX31_RS09555 at 116.877 kb on + strand at 116.877 kb on + strand at 116.877 kb on + strand at 116.877 kb on + strand at 116.877 kb on + strand at 116.877 kb on + strand at 116.877 kb on + strand at 116.878 kb on - strand at 116.878 kb on - strand at 116.878 kb on - strand at 116.878 kb on - strand at 116.878 kb on - strand at 116.930 kb on - strand at 116.940 kb on + strand at 117.075 kb on + strand, within EX31_RS09560 at 117.075 kb on + strand, within EX31_RS09560 at 117.087 kb on + strand, within EX31_RS09560 at 117.202 kb on + strand, within EX31_RS09560 at 117.202 kb on + strand, within EX31_RS09560 at 117.202 kb on + strand, within EX31_RS09560 at 117.274 kb on - strand, within EX31_RS09560 at 117.464 kb on - strand at 117.466 kb on + strand at 117.467 kb on - strand at 117.583 kb on + strand, within EX31_RS09565
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 114,674 + EX31_RS09545 0.89 -0.3 114,674 + EX31_RS09545 0.89 -0.1 114,674 + EX31_RS09545 0.89 -0.9 114,674 + EX31_RS09545 0.89 -1.0 114,675 - EX31_RS09545 0.89 -1.6 114,675 - EX31_RS09545 0.89 -1.8 114,771 + -0.5 114,772 - -1.6 114,772 - -0.0 114,786 + +1.0 114,786 + +0.3 114,790 - +0.1 114,790 - -0.5 114,987 - EX31_RS09550 0.27 -1.0 115,170 + EX31_RS09550 0.51 +0.4 115,171 - EX31_RS09550 0.51 +0.8 115,320 + EX31_RS09550 0.70 -2.0 115,320 + EX31_RS09550 0.70 -1.9 115,694 - EX31_RS09555 0.10 +1.8 115,839 + EX31_RS09555 0.21 +0.3 115,839 + EX31_RS09555 0.21 -1.0 115,839 + EX31_RS09555 0.21 -2.6 115,839 + EX31_RS09555 0.21 -0.8 115,915 + EX31_RS09555 0.26 +0.8 115,915 + EX31_RS09555 0.26 -1.7 115,915 + EX31_RS09555 0.26 -0.9 115,915 + EX31_RS09555 0.26 -1.5 115,915 + EX31_RS09555 0.26 -1.9 116,287 + EX31_RS09555 0.53 -1.0 116,287 + EX31_RS09555 0.53 +1.1 116,287 + EX31_RS09555 0.53 +0.4 116,287 + EX31_RS09555 0.53 +0.7 116,288 - EX31_RS09555 0.54 -0.7 116,288 - EX31_RS09555 0.54 +0.3 116,291 + EX31_RS09555 0.54 -2.7 116,291 + EX31_RS09555 0.54 -1.7 116,291 + EX31_RS09555 0.54 -1.3 116,292 - EX31_RS09555 0.54 +1.1 116,292 - EX31_RS09555 0.54 -0.0 116,353 + EX31_RS09555 0.58 -1.3 116,443 - EX31_RS09555 0.65 -2.9 116,530 - EX31_RS09555 0.71 +1.8 116,530 - EX31_RS09555 0.71 -0.2 116,536 + EX31_RS09555 0.72 -0.4 116,536 + EX31_RS09555 0.72 -0.3 116,537 - EX31_RS09555 0.72 -0.7 116,690 + EX31_RS09555 0.83 -0.3 116,745 + EX31_RS09555 0.87 -1.7 116,746 - EX31_RS09555 0.87 -1.0 116,781 + EX31_RS09555 0.90 -0.1 116,877 + -1.5 116,877 + -0.5 116,877 + -0.0 116,877 + -0.4 116,877 + +0.2 116,877 + -0.3 116,877 + +1.8 116,878 - +0.3 116,878 - -0.6 116,878 - -2.0 116,878 - +1.3 116,878 - +0.1 116,930 - +0.3 116,940 + +0.8 117,075 + EX31_RS09560 0.30 +0.6 117,075 + EX31_RS09560 0.30 +0.3 117,087 + EX31_RS09560 0.32 +1.1 117,202 + EX31_RS09560 0.58 -2.4 117,202 + EX31_RS09560 0.58 -1.3 117,202 + EX31_RS09560 0.58 +0.3 117,274 - EX31_RS09560 0.74 -0.2 117,464 - +0.6 117,466 + -1.2 117,467 - +0.3 117,583 + EX31_RS09565 0.14 +0.3
Or see this region's nucleotide sequence