Strain Fitness in Rahnella sp. WP5 around EX31_RS03785

Experiment: Ammonium chloride (N); ShewMM media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS03775 and EX31_RS03780 are separated by 5 nucleotidesEX31_RS03780 and EX31_RS03785 are separated by 4 nucleotidesEX31_RS03785 and EX31_RS03790 are separated by 5 nucleotidesEX31_RS03790 and EX31_RS03795 are separated by 82 nucleotides EX31_RS03775: EX31_RS03775 - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 264,152 to 265,159 _RS03775 EX31_RS03780: EX31_RS03780 - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA, at 265,165 to 265,992 _RS03780 EX31_RS03785: EX31_RS03785 - Co2+/Mg2+ efflux protein ApaG, at 265,997 to 266,374 _RS03785 EX31_RS03790: EX31_RS03790 - bis(5'-nucleosyl)-tetraphosphatase (symmetrical), at 266,380 to 267,234 _RS03790 EX31_RS03795: EX31_RS03795 - type 3 dihydrofolate reductase, at 267,317 to 267,802 _RS03795 Position (kb) 265 266 267Strain fitness (log2 ratio) -2 -1 0 1 2at 265.012 kb on + strand, within EX31_RS03775at 265.141 kb on + strandat 265.990 kb on + strandat 265.996 kb on + strandat 265.997 kb on - strandat 266.002 kb on + strandat 266.002 kb on + strandat 266.002 kb on + strandat 266.002 kb on + strandat 266.002 kb on + strandat 266.003 kb on - strandat 266.003 kb on - strandat 266.003 kb on - strandat 266.035 kb on + strand, within EX31_RS03785at 266.035 kb on + strand, within EX31_RS03785at 266.035 kb on + strand, within EX31_RS03785at 266.035 kb on + strand, within EX31_RS03785at 266.035 kb on + strand, within EX31_RS03785at 266.035 kb on + strand, within EX31_RS03785at 266.036 kb on - strand, within EX31_RS03785at 266.039 kb on + strand, within EX31_RS03785at 266.039 kb on + strand, within EX31_RS03785at 266.039 kb on + strand, within EX31_RS03785at 266.039 kb on + strand, within EX31_RS03785at 266.039 kb on + strand, within EX31_RS03785at 266.039 kb on + strand, within EX31_RS03785at 266.040 kb on - strand, within EX31_RS03785at 266.040 kb on - strand, within EX31_RS03785at 266.087 kb on + strand, within EX31_RS03785at 266.087 kb on + strand, within EX31_RS03785at 266.087 kb on + strand, within EX31_RS03785at 266.119 kb on + strand, within EX31_RS03785at 266.119 kb on + strand, within EX31_RS03785at 266.120 kb on - strand, within EX31_RS03785at 266.151 kb on + strand, within EX31_RS03785at 266.161 kb on + strand, within EX31_RS03785at 266.240 kb on + strand, within EX31_RS03785at 266.240 kb on + strand, within EX31_RS03785at 266.242 kb on + strand, within EX31_RS03785at 266.242 kb on + strand, within EX31_RS03785at 266.242 kb on + strand, within EX31_RS03785at 266.291 kb on + strand, within EX31_RS03785at 266.291 kb on + strand, within EX31_RS03785at 266.291 kb on + strand, within EX31_RS03785at 266.292 kb on - strand, within EX31_RS03785at 266.359 kb on + strandat 266.360 kb on - strandat 266.368 kb on - strandat 266.372 kb on + strandat 266.389 kb on + strandat 266.390 kb on - strandat 266.394 kb on + strandat 266.818 kb on - strand, within EX31_RS03790at 266.822 kb on - strand, within EX31_RS03790at 266.845 kb on + strand, within EX31_RS03790at 266.845 kb on + strand, within EX31_RS03790at 266.846 kb on - strand, within EX31_RS03790at 267.048 kb on + strand, within EX31_RS03790at 267.103 kb on + strand, within EX31_RS03790at 267.104 kb on - strand, within EX31_RS03790at 267.169 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking
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265,012 + EX31_RS03775 0.85 -0.2
265,141 + +0.5
265,990 + +2.0
265,996 + -2.0
265,997 - -0.2
266,002 + -1.7
266,002 + -0.4
266,002 + -1.6
266,002 + +1.4
266,002 + -0.3
266,003 - +0.6
266,003 - -0.3
266,003 - -2.0
266,035 + EX31_RS03785 0.10 +0.0
266,035 + EX31_RS03785 0.10 +0.3
266,035 + EX31_RS03785 0.10 +0.5
266,035 + EX31_RS03785 0.10 -0.1
266,035 + EX31_RS03785 0.10 +0.0
266,035 + EX31_RS03785 0.10 +0.5
266,036 - EX31_RS03785 0.10 -1.3
266,039 + EX31_RS03785 0.11 +0.2
266,039 + EX31_RS03785 0.11 +0.7
266,039 + EX31_RS03785 0.11 +0.8
266,039 + EX31_RS03785 0.11 +0.7
266,039 + EX31_RS03785 0.11 -0.6
266,039 + EX31_RS03785 0.11 +0.6
266,040 - EX31_RS03785 0.11 +0.2
266,040 - EX31_RS03785 0.11 -1.2
266,087 + EX31_RS03785 0.24 +0.6
266,087 + EX31_RS03785 0.24 -0.5
266,087 + EX31_RS03785 0.24 -0.4
266,119 + EX31_RS03785 0.32 +1.2
266,119 + EX31_RS03785 0.32 -0.2
266,120 - EX31_RS03785 0.33 -0.0
266,151 + EX31_RS03785 0.41 -0.0
266,161 + EX31_RS03785 0.43 +0.2
266,240 + EX31_RS03785 0.64 -0.3
266,240 + EX31_RS03785 0.64 -0.4
266,242 + EX31_RS03785 0.65 +0.4
266,242 + EX31_RS03785 0.65 -0.3
266,242 + EX31_RS03785 0.65 -1.2
266,291 + EX31_RS03785 0.78 +0.2
266,291 + EX31_RS03785 0.78 -0.2
266,291 + EX31_RS03785 0.78 -0.3
266,292 - EX31_RS03785 0.78 -0.0
266,359 + -0.4
266,360 - -1.3
266,368 - -1.3
266,372 + -0.3
266,389 + -0.4
266,390 - -0.2
266,394 + -2.7
266,818 - EX31_RS03790 0.51 +0.6
266,822 - EX31_RS03790 0.52 -0.2
266,845 + EX31_RS03790 0.54 -2.1
266,845 + EX31_RS03790 0.54 -0.4
266,846 - EX31_RS03790 0.55 -2.2
267,048 + EX31_RS03790 0.78 +0.2
267,103 + EX31_RS03790 0.85 -2.0
267,104 - EX31_RS03790 0.85 -1.2
267,169 + -0.5

Or see this region's nucleotide sequence