Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02025 and EX31_RS02030 overlap by 20 nucleotides EX31_RS02030 and EX31_RS02035 overlap by 17 nucleotides EX31_RS02035 and EX31_RS02040 overlap by 4 nucleotides
EX31_RS02025: EX31_RS02025 - ABC transporter substrate-binding protein, at 443,770 to 445,284
_RS02025
EX31_RS02030: EX31_RS02030 - ATP-binding cassette domain-containing protein, at 445,265 to 446,314
_RS02030
EX31_RS02035: EX31_RS02035 - ABC transporter ATP-binding protein, at 446,298 to 447,275
_RS02035
EX31_RS02040: EX31_RS02040 - ABC transporter permease subunit, at 447,272 to 448,132
_RS02040
Position (kb) 445
446
447 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 444.320 kb on + strand, within EX31_RS02025 at 444.321 kb on - strand, within EX31_RS02025 at 444.321 kb on - strand, within EX31_RS02025 at 444.326 kb on + strand, within EX31_RS02025 at 444.326 kb on + strand, within EX31_RS02025 at 444.327 kb on - strand, within EX31_RS02025 at 444.327 kb on - strand, within EX31_RS02025 at 444.347 kb on + strand, within EX31_RS02025 at 444.348 kb on - strand, within EX31_RS02025 at 444.402 kb on + strand, within EX31_RS02025 at 444.669 kb on - strand, within EX31_RS02025 at 444.894 kb on + strand, within EX31_RS02025 at 444.902 kb on + strand, within EX31_RS02025 at 444.902 kb on + strand, within EX31_RS02025 at 444.903 kb on - strand, within EX31_RS02025 at 445.018 kb on - strand, within EX31_RS02025 at 445.018 kb on - strand, within EX31_RS02025 at 445.019 kb on + strand, within EX31_RS02025 at 445.019 kb on + strand, within EX31_RS02025 at 445.019 kb on + strand, within EX31_RS02025 at 445.020 kb on - strand, within EX31_RS02025 at 445.020 kb on - strand, within EX31_RS02025 at 445.020 kb on - strand, within EX31_RS02025 at 445.020 kb on - strand, within EX31_RS02025 at 445.198 kb on + strand at 445.267 kb on - strand at 445.287 kb on - strand at 445.287 kb on - strand at 445.364 kb on - strand at 445.364 kb on - strand at 445.542 kb on + strand, within EX31_RS02030 at 445.542 kb on + strand, within EX31_RS02030 at 445.543 kb on - strand, within EX31_RS02030 at 445.543 kb on - strand, within EX31_RS02030 at 445.543 kb on - strand, within EX31_RS02030 at 445.543 kb on - strand, within EX31_RS02030 at 445.594 kb on + strand, within EX31_RS02030 at 445.594 kb on + strand, within EX31_RS02030 at 445.595 kb on - strand, within EX31_RS02030 at 445.595 kb on - strand, within EX31_RS02030 at 445.595 kb on - strand, within EX31_RS02030 at 445.595 kb on - strand, within EX31_RS02030 at 445.595 kb on - strand, within EX31_RS02030 at 445.605 kb on + strand, within EX31_RS02030 at 445.605 kb on + strand, within EX31_RS02030 at 445.605 kb on + strand, within EX31_RS02030 at 445.606 kb on - strand, within EX31_RS02030 at 445.606 kb on - strand, within EX31_RS02030 at 445.660 kb on + strand, within EX31_RS02030 at 445.661 kb on - strand, within EX31_RS02030 at 445.664 kb on + strand, within EX31_RS02030 at 445.665 kb on - strand, within EX31_RS02030 at 445.665 kb on - strand, within EX31_RS02030 at 445.713 kb on - strand, within EX31_RS02030 at 445.713 kb on - strand, within EX31_RS02030 at 446.534 kb on + strand, within EX31_RS02035 at 446.537 kb on - strand, within EX31_RS02035 at 446.599 kb on + strand, within EX31_RS02035 at 446.600 kb on - strand, within EX31_RS02035 at 446.652 kb on - strand, within EX31_RS02035 at 446.661 kb on - strand, within EX31_RS02035 at 446.834 kb on - strand, within EX31_RS02035 at 446.834 kb on - strand, within EX31_RS02035 at 446.837 kb on + strand, within EX31_RS02035 at 446.838 kb on - strand, within EX31_RS02035 at 446.838 kb on - strand, within EX31_RS02035 at 446.838 kb on - strand, within EX31_RS02035
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 444,320 + EX31_RS02025 0.36 +1.1 444,321 - EX31_RS02025 0.36 +0.4 444,321 - EX31_RS02025 0.36 -0.2 444,326 + EX31_RS02025 0.37 -0.7 444,326 + EX31_RS02025 0.37 +0.1 444,327 - EX31_RS02025 0.37 +0.6 444,327 - EX31_RS02025 0.37 +0.0 444,347 + EX31_RS02025 0.38 +0.6 444,348 - EX31_RS02025 0.38 -1.4 444,402 + EX31_RS02025 0.42 -0.3 444,669 - EX31_RS02025 0.59 +2.1 444,894 + EX31_RS02025 0.74 +2.7 444,902 + EX31_RS02025 0.75 +0.7 444,902 + EX31_RS02025 0.75 -1.7 444,903 - EX31_RS02025 0.75 +0.1 445,018 - EX31_RS02025 0.82 -0.3 445,018 - EX31_RS02025 0.82 +0.3 445,019 + EX31_RS02025 0.82 -0.9 445,019 + EX31_RS02025 0.82 +1.6 445,019 + EX31_RS02025 0.82 +0.6 445,020 - EX31_RS02025 0.83 -0.2 445,020 - EX31_RS02025 0.83 +1.6 445,020 - EX31_RS02025 0.83 -1.0 445,020 - EX31_RS02025 0.83 +1.6 445,198 + -1.6 445,267 - +0.6 445,287 - -0.2 445,287 - -0.7 445,364 - +0.0 445,364 - -0.3 445,542 + EX31_RS02030 0.26 +1.6 445,542 + EX31_RS02030 0.26 +1.1 445,543 - EX31_RS02030 0.26 +2.6 445,543 - EX31_RS02030 0.26 -2.3 445,543 - EX31_RS02030 0.26 +1.8 445,543 - EX31_RS02030 0.26 -0.5 445,594 + EX31_RS02030 0.31 -1.2 445,594 + EX31_RS02030 0.31 +0.6 445,595 - EX31_RS02030 0.31 -1.0 445,595 - EX31_RS02030 0.31 -0.2 445,595 - EX31_RS02030 0.31 -2.7 445,595 - EX31_RS02030 0.31 -1.2 445,595 - EX31_RS02030 0.31 -0.3 445,605 + EX31_RS02030 0.32 +3.1 445,605 + EX31_RS02030 0.32 -0.6 445,605 + EX31_RS02030 0.32 +0.0 445,606 - EX31_RS02030 0.32 -1.3 445,606 - EX31_RS02030 0.32 +1.7 445,660 + EX31_RS02030 0.38 +0.6 445,661 - EX31_RS02030 0.38 -0.8 445,664 + EX31_RS02030 0.38 +0.6 445,665 - EX31_RS02030 0.38 -0.4 445,665 - EX31_RS02030 0.38 -1.6 445,713 - EX31_RS02030 0.43 +0.6 445,713 - EX31_RS02030 0.43 -1.8 446,534 + EX31_RS02035 0.24 +0.2 446,537 - EX31_RS02035 0.24 +0.0 446,599 + EX31_RS02035 0.31 -1.6 446,600 - EX31_RS02035 0.31 +0.3 446,652 - EX31_RS02035 0.36 -1.3 446,661 - EX31_RS02035 0.37 +0.1 446,834 - EX31_RS02035 0.55 -0.3 446,834 - EX31_RS02035 0.55 +0.1 446,837 + EX31_RS02035 0.55 +0.4 446,838 - EX31_RS02035 0.55 -0.7 446,838 - EX31_RS02035 0.55 +3.7 446,838 - EX31_RS02035 0.55 -0.2
Or see this region's nucleotide sequence