Experiment: Ammonium chloride (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00245 and EX31_RS00250 are separated by 195 nucleotides EX31_RS00250 and EX31_RS00255 are separated by 71 nucleotides EX31_RS00255 and EX31_RS00260 are separated by 141 nucleotides
EX31_RS00245: EX31_RS00245 - HTH-type transcriptional activator RhaS, at 50,710 to 51,534
_RS00245
EX31_RS00250: EX31_RS00250 - aldehyde dehydrogenase, at 51,730 to 53,169
_RS00250
EX31_RS00255: EX31_RS00255 - hypothetical protein, at 53,241 to 53,822
_RS00255
EX31_RS00260: EX31_RS00260 - hypothetical protein, at 53,964 to 55,040
_RS00260
Position (kb) 51
52
53
54 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 50.968 kb on + strand, within EX31_RS00245 at 51.096 kb on + strand, within EX31_RS00245 at 51.096 kb on + strand, within EX31_RS00245 at 51.155 kb on + strand, within EX31_RS00245 at 51.156 kb on - strand, within EX31_RS00245 at 51.156 kb on - strand, within EX31_RS00245 at 51.158 kb on - strand, within EX31_RS00245 at 51.162 kb on + strand, within EX31_RS00245 at 51.173 kb on + strand, within EX31_RS00245 at 51.173 kb on + strand, within EX31_RS00245 at 51.174 kb on - strand, within EX31_RS00245 at 51.176 kb on + strand, within EX31_RS00245 at 51.262 kb on + strand, within EX31_RS00245 at 51.263 kb on - strand, within EX31_RS00245 at 51.263 kb on - strand, within EX31_RS00245 at 51.469 kb on + strand at 51.469 kb on + strand at 51.469 kb on + strand at 51.469 kb on + strand at 51.470 kb on - strand at 51.526 kb on + strand at 51.547 kb on - strand at 51.547 kb on - strand at 51.562 kb on + strand at 51.562 kb on + strand at 51.607 kb on + strand at 51.608 kb on - strand at 51.608 kb on - strand at 51.608 kb on - strand at 51.713 kb on - strand at 51.727 kb on + strand at 51.727 kb on + strand at 51.727 kb on + strand at 51.727 kb on + strand at 51.728 kb on - strand at 51.728 kb on - strand at 51.728 kb on - strand at 51.728 kb on - strand at 51.728 kb on - strand at 51.728 kb on - strand at 51.750 kb on + strand at 51.750 kb on + strand at 51.750 kb on + strand at 51.750 kb on + strand at 51.750 kb on + strand at 51.750 kb on + strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.755 kb on - strand at 51.755 kb on - strand at 51.755 kb on - strand at 51.755 kb on - strand at 51.755 kb on - strand at 51.755 kb on - strand at 51.755 kb on - strand at 51.945 kb on + strand, within EX31_RS00250 at 52.019 kb on - strand, within EX31_RS00250 at 52.041 kb on - strand, within EX31_RS00250 at 52.041 kb on - strand, within EX31_RS00250 at 52.058 kb on - strand, within EX31_RS00250 at 52.058 kb on - strand, within EX31_RS00250 at 52.066 kb on + strand, within EX31_RS00250 at 52.066 kb on + strand, within EX31_RS00250 at 52.066 kb on + strand, within EX31_RS00250 at 52.066 kb on + strand, within EX31_RS00250 at 52.066 kb on + strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.239 kb on + strand, within EX31_RS00250 at 52.240 kb on - strand, within EX31_RS00250 at 52.240 kb on - strand, within EX31_RS00250 at 52.300 kb on - strand, within EX31_RS00250 at 52.335 kb on + strand, within EX31_RS00250 at 52.386 kb on + strand, within EX31_RS00250 at 52.581 kb on + strand, within EX31_RS00250 at 52.581 kb on + strand, within EX31_RS00250 at 52.582 kb on - strand, within EX31_RS00250 at 52.600 kb on + strand, within EX31_RS00250 at 52.600 kb on + strand, within EX31_RS00250 at 52.600 kb on + strand, within EX31_RS00250 at 52.600 kb on + strand, within EX31_RS00250 at 52.601 kb on - strand, within EX31_RS00250 at 52.616 kb on + strand, within EX31_RS00250 at 52.744 kb on - strand, within EX31_RS00250 at 52.972 kb on + strand, within EX31_RS00250 at 52.972 kb on + strand, within EX31_RS00250 at 52.973 kb on - strand, within EX31_RS00250 at 52.983 kb on + strand, within EX31_RS00250 at 52.984 kb on - strand, within EX31_RS00250 at 52.984 kb on - strand, within EX31_RS00250 at 52.985 kb on + strand, within EX31_RS00250 at 52.986 kb on - strand, within EX31_RS00250 at 52.986 kb on - strand, within EX31_RS00250 at 52.986 kb on - strand, within EX31_RS00250 at 53.032 kb on + strand at 53.032 kb on + strand at 53.085 kb on + strand at 53.131 kb on + strand at 53.168 kb on - strand at 53.171 kb on + strand at 53.172 kb on - strand at 53.280 kb on - strand at 53.424 kb on + strand, within EX31_RS00255 at 53.425 kb on - strand, within EX31_RS00255 at 53.437 kb on + strand, within EX31_RS00255 at 53.437 kb on + strand, within EX31_RS00255 at 53.438 kb on - strand, within EX31_RS00255 at 53.438 kb on - strand, within EX31_RS00255 at 53.438 kb on - strand, within EX31_RS00255 at 53.438 kb on - strand, within EX31_RS00255 at 53.439 kb on + strand, within EX31_RS00255 at 53.440 kb on - strand, within EX31_RS00255 at 53.440 kb on - strand, within EX31_RS00255 at 53.481 kb on - strand, within EX31_RS00255 at 53.722 kb on - strand, within EX31_RS00255 at 53.729 kb on + strand, within EX31_RS00255 at 53.730 kb on - strand, within EX31_RS00255 at 53.730 kb on - strand, within EX31_RS00255 at 53.730 kb on - strand, within EX31_RS00255 at 53.820 kb on + strand at 53.821 kb on - strand at 53.862 kb on - strand at 53.875 kb on + strand at 53.875 kb on + strand at 53.884 kb on + strand at 53.891 kb on + strand at 53.974 kb on + strand at 54.068 kb on + strand at 54.069 kb on - strand at 54.137 kb on + strand, within EX31_RS00260 at 54.137 kb on + strand, within EX31_RS00260 at 54.159 kb on + strand, within EX31_RS00260 at 54.161 kb on + strand, within EX31_RS00260 at 54.161 kb on + strand, within EX31_RS00260 at 54.162 kb on - strand, within EX31_RS00260
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); ShewMM media; no shaking remove 50,968 + EX31_RS00245 0.31 +0.4 51,096 + EX31_RS00245 0.47 -1.0 51,096 + EX31_RS00245 0.47 -1.7 51,155 + EX31_RS00245 0.54 -0.1 51,156 - EX31_RS00245 0.54 +0.8 51,156 - EX31_RS00245 0.54 +0.6 51,158 - EX31_RS00245 0.54 -1.1 51,162 + EX31_RS00245 0.55 -0.2 51,173 + EX31_RS00245 0.56 +2.1 51,173 + EX31_RS00245 0.56 -0.0 51,174 - EX31_RS00245 0.56 +0.3 51,176 + EX31_RS00245 0.56 +0.1 51,262 + EX31_RS00245 0.67 -1.6 51,263 - EX31_RS00245 0.67 +0.8 51,263 - EX31_RS00245 0.67 -0.2 51,469 + -0.5 51,469 + -1.2 51,469 + +0.8 51,469 + +0.9 51,470 - -1.7 51,526 + +0.1 51,547 - +0.6 51,547 - +1.0 51,562 + +0.6 51,562 + +0.3 51,607 + +0.9 51,608 - -2.0 51,608 - -0.4 51,608 - -0.7 51,713 - +1.4 51,727 + +1.2 51,727 + -0.1 51,727 + -2.0 51,727 + +0.4 51,728 - +0.3 51,728 - -0.1 51,728 - +1.1 51,728 - -0.8 51,728 - -2.4 51,728 - +1.7 51,750 + -2.0 51,750 + +0.5 51,750 + -0.1 51,750 + -1.1 51,750 + +1.2 51,750 + +1.4 51,751 - -1.3 51,751 - +0.6 51,751 - -0.5 51,751 - +2.1 51,751 - +0.1 51,751 - +0.6 51,751 - +3.7 51,751 - -1.2 51,751 - +1.7 51,751 - -0.6 51,754 + +0.5 51,754 + +0.5 51,754 + +0.8 51,754 + -0.5 51,754 + -0.0 51,754 + -0.6 51,754 + -0.5 51,754 + -1.0 51,754 + +1.3 51,754 + +1.2 51,754 + +0.5 51,754 + -0.7 51,755 - -0.1 51,755 - -1.6 51,755 - +1.1 51,755 - -1.7 51,755 - -1.6 51,755 - +0.3 51,755 - -0.5 51,945 + EX31_RS00250 0.15 +1.3 52,019 - EX31_RS00250 0.20 +0.7 52,041 - EX31_RS00250 0.22 +0.8 52,041 - EX31_RS00250 0.22 +0.3 52,058 - EX31_RS00250 0.23 +0.8 52,058 - EX31_RS00250 0.23 -1.4 52,066 + EX31_RS00250 0.23 -0.2 52,066 + EX31_RS00250 0.23 -0.0 52,066 + EX31_RS00250 0.23 -0.2 52,066 + EX31_RS00250 0.23 +0.9 52,066 + EX31_RS00250 0.23 -0.0 52,067 - EX31_RS00250 0.23 +0.6 52,067 - EX31_RS00250 0.23 +1.7 52,067 - EX31_RS00250 0.23 -0.1 52,067 - EX31_RS00250 0.23 -0.5 52,067 - EX31_RS00250 0.23 +1.1 52,067 - EX31_RS00250 0.23 +0.8 52,067 - EX31_RS00250 0.23 +0.3 52,067 - EX31_RS00250 0.23 -0.4 52,067 - EX31_RS00250 0.23 -0.1 52,067 - EX31_RS00250 0.23 +0.3 52,239 + EX31_RS00250 0.35 +1.3 52,240 - EX31_RS00250 0.35 +1.2 52,240 - EX31_RS00250 0.35 +0.7 52,300 - EX31_RS00250 0.40 +0.5 52,335 + EX31_RS00250 0.42 -0.7 52,386 + EX31_RS00250 0.46 +0.2 52,581 + EX31_RS00250 0.59 +1.1 52,581 + EX31_RS00250 0.59 +1.7 52,582 - EX31_RS00250 0.59 -0.2 52,600 + EX31_RS00250 0.60 +0.4 52,600 + EX31_RS00250 0.60 +0.5 52,600 + EX31_RS00250 0.60 -0.1 52,600 + EX31_RS00250 0.60 +2.1 52,601 - EX31_RS00250 0.60 -0.4 52,616 + EX31_RS00250 0.62 +0.1 52,744 - EX31_RS00250 0.70 -0.8 52,972 + EX31_RS00250 0.86 -0.0 52,972 + EX31_RS00250 0.86 +0.6 52,973 - EX31_RS00250 0.86 +0.2 52,983 + EX31_RS00250 0.87 +0.3 52,984 - EX31_RS00250 0.87 +1.1 52,984 - EX31_RS00250 0.87 +0.6 52,985 + EX31_RS00250 0.87 +0.2 52,986 - EX31_RS00250 0.87 -1.5 52,986 - EX31_RS00250 0.87 -0.7 52,986 - EX31_RS00250 0.87 +0.6 53,032 + -0.9 53,032 + +1.1 53,085 + +1.5 53,131 + +0.7 53,168 - +1.5 53,171 + -0.0 53,172 - +0.7 53,280 - -0.2 53,424 + EX31_RS00255 0.31 +0.7 53,425 - EX31_RS00255 0.32 -1.5 53,437 + EX31_RS00255 0.34 +0.3 53,437 + EX31_RS00255 0.34 +2.1 53,438 - EX31_RS00255 0.34 +0.1 53,438 - EX31_RS00255 0.34 -1.2 53,438 - EX31_RS00255 0.34 +2.4 53,438 - EX31_RS00255 0.34 +0.4 53,439 + EX31_RS00255 0.34 -0.5 53,440 - EX31_RS00255 0.34 +0.4 53,440 - EX31_RS00255 0.34 -0.6 53,481 - EX31_RS00255 0.41 -0.2 53,722 - EX31_RS00255 0.83 +0.1 53,729 + EX31_RS00255 0.84 -0.9 53,730 - EX31_RS00255 0.84 +0.9 53,730 - EX31_RS00255 0.84 +0.4 53,730 - EX31_RS00255 0.84 -0.1 53,820 + +1.5 53,821 - -0.3 53,862 - +0.5 53,875 + -1.7 53,875 + -0.5 53,884 + +0.8 53,891 + +0.2 53,974 + -0.1 54,068 + +0.5 54,069 - -1.2 54,137 + EX31_RS00260 0.16 -0.9 54,137 + EX31_RS00260 0.16 -0.4 54,159 + EX31_RS00260 0.18 +0.5 54,161 + EX31_RS00260 0.18 +1.7 54,161 + EX31_RS00260 0.18 -0.7 54,162 - EX31_RS00260 0.18 +0.2
Or see this region's nucleotide sequence