Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_666 and Ac3H11_667 are separated by 59 nucleotides Ac3H11_667 and Ac3H11_668 overlap by 4 nucleotides Ac3H11_668 and Ac3H11_669 are separated by 40 nucleotides
Ac3H11_666 - Bacteriophage-related DNA polymerase, at 23,509 to 24,279
_666
Ac3H11_667 - Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-), at 24,339 to 24,842
_667
Ac3H11_668 - TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA, at 24,839 to 25,576
_668
Ac3H11_669 - Permease of the drug/metabolite transporter (DMT) superfamily, at 25,617 to 26,612
_669
Position (kb) 24
25 Strain fitness (log2 ratio) -1
0
1 at 23.410 kb on - strand at 23.410 kb on - strand at 23.422 kb on - strand at 23.469 kb on - strand at 24.280 kb on - strand at 24.280 kb on - strand at 24.280 kb on - strand at 24.280 kb on - strand at 24.340 kb on + strand at 24.395 kb on - strand, within Ac3H11_667 at 24.395 kb on - strand, within Ac3H11_667 at 24.397 kb on + strand, within Ac3H11_667 at 24.398 kb on - strand, within Ac3H11_667 at 24.398 kb on - strand, within Ac3H11_667 at 24.439 kb on + strand, within Ac3H11_667 at 24.439 kb on + strand, within Ac3H11_667 at 24.440 kb on - strand, within Ac3H11_667 at 24.716 kb on - strand, within Ac3H11_667
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 23,410 - +0.8 23,410 - -0.3 23,422 - -0.7 23,469 - -0.9 24,280 - -0.8 24,280 - +0.6 24,280 - +0.2 24,280 - -0.5 24,340 + -0.3 24,395 - Ac3H11_667 0.11 -0.3 24,395 - Ac3H11_667 0.11 +0.4 24,397 + Ac3H11_667 0.12 -0.8 24,398 - Ac3H11_667 0.12 -0.6 24,398 - Ac3H11_667 0.12 -0.6 24,439 + Ac3H11_667 0.20 -0.6 24,439 + Ac3H11_667 0.20 +0.5 24,440 - Ac3H11_667 0.20 +0.1 24,716 - Ac3H11_667 0.75 -0.8
Or see this region's nucleotide sequence