Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1313 and Ac3H11_1314 are separated by 13 nucleotides Ac3H11_1314 and Ac3H11_1315 are separated by 35 nucleotides Ac3H11_1315 and Ac3H11_1316 overlap by 4 nucleotides Ac3H11_1316 and Ac3H11_1317 are separated by 125 nucleotides
Ac3H11_1313 - Poly(A) polymerase (EC 2.7.7.19), at 903 to 2,513
_1313
Ac3H11_1314 - Phosphoserine phosphatase (EC 3.1.3.3), at 2,527 to 3,237
_1314
Ac3H11_1315 - Chromosomal replication initiator protein DnaA, at 3,273 to 3,977
_1315
Ac3H11_1316 - hypothetical protein, at 3,974 to 4,117
_1316
Ac3H11_1317 - Permeases of the major facilitator superfamily, at 4,243 to 5,460
_1317
Position (kb) 3
4 Strain fitness (log2 ratio) -1
0
1 at 2.518 kb on - strand at 2.525 kb on - strand at 2.570 kb on - strand at 4.185 kb on + strand at 4.185 kb on + strand at 4.186 kb on - strand at 4.186 kb on - strand at 4.502 kb on - strand, within Ac3H11_1317 at 4.502 kb on - strand, within Ac3H11_1317 at 4.897 kb on + strand, within Ac3H11_1317 at 4.897 kb on + strand, within Ac3H11_1317 at 4.897 kb on + strand, within Ac3H11_1317 at 4.897 kb on + strand, within Ac3H11_1317 at 4.898 kb on - strand, within Ac3H11_1317
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 2,518 - -0.6 2,525 - +0.6 2,570 - -1.6 4,185 + -1.1 4,185 + +0.1 4,186 - -0.1 4,186 - +0.7 4,502 - Ac3H11_1317 0.21 -0.1 4,502 - Ac3H11_1317 0.21 -0.4 4,897 + Ac3H11_1317 0.54 +0.4 4,897 + Ac3H11_1317 0.54 -0.0 4,897 + Ac3H11_1317 0.54 +1.3 4,897 + Ac3H11_1317 0.54 +0.6 4,898 - Ac3H11_1317 0.54 -0.4
Or see this region's nucleotide sequence