Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS24055 and EX31_RS24060 are separated by 254 nucleotides EX31_RS24060 and EX31_RS24065 are separated by 31 nucleotides EX31_RS24065 and EX31_RS24070 are separated by 30 nucleotides
EX31_RS24055: EX31_RS24055 - TIGR04028 family ABC transporter substrate-binding protein, at 579,601 to 581,241
_RS24055
EX31_RS24060: EX31_RS24060 - pyrimidine utilization transport protein G, at 581,496 to 582,845
_RS24060
EX31_RS24065: EX31_RS24065 - pyrimidine utilization flavin reductase protein F, at 582,877 to 583,503
_RS24065
EX31_RS24070: EX31_RS24070 - malonic semialdehyde reductase, at 583,534 to 584,124
_RS24070
Position (kb) 581
582
583 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 580.580 kb on + strand, within EX31_RS24055 at 580.580 kb on + strand, within EX31_RS24055 at 580.686 kb on + strand, within EX31_RS24055 at 580.725 kb on + strand, within EX31_RS24055 at 580.726 kb on - strand, within EX31_RS24055 at 580.726 kb on - strand, within EX31_RS24055 at 580.769 kb on + strand, within EX31_RS24055 at 580.769 kb on + strand, within EX31_RS24055 at 580.770 kb on - strand, within EX31_RS24055 at 580.811 kb on + strand, within EX31_RS24055 at 580.811 kb on + strand, within EX31_RS24055 at 580.812 kb on - strand, within EX31_RS24055 at 580.865 kb on + strand, within EX31_RS24055 at 580.865 kb on + strand, within EX31_RS24055 at 580.866 kb on - strand, within EX31_RS24055 at 580.866 kb on - strand, within EX31_RS24055 at 580.869 kb on + strand, within EX31_RS24055 at 580.869 kb on + strand, within EX31_RS24055 at 580.870 kb on - strand, within EX31_RS24055 at 580.870 kb on - strand, within EX31_RS24055 at 580.870 kb on - strand, within EX31_RS24055 at 580.870 kb on - strand, within EX31_RS24055 at 580.949 kb on + strand, within EX31_RS24055 at 580.949 kb on + strand, within EX31_RS24055 at 580.950 kb on - strand, within EX31_RS24055 at 580.959 kb on + strand, within EX31_RS24055 at 580.960 kb on - strand, within EX31_RS24055 at 580.960 kb on - strand, within EX31_RS24055 at 581.024 kb on + strand, within EX31_RS24055 at 581.069 kb on + strand, within EX31_RS24055 at 581.120 kb on + strand at 581.120 kb on + strand at 581.245 kb on + strand at 581.296 kb on + strand at 581.296 kb on + strand at 581.296 kb on + strand at 581.297 kb on - strand at 581.297 kb on - strand at 581.297 kb on - strand at 581.297 kb on - strand at 581.331 kb on + strand at 581.331 kb on + strand at 581.332 kb on - strand at 581.332 kb on - strand at 581.477 kb on + strand at 581.477 kb on + strand at 581.477 kb on + strand at 581.478 kb on - strand at 581.478 kb on - strand at 581.478 kb on - strand at 581.478 kb on - strand at 581.478 kb on - strand at 581.509 kb on - strand at 581.788 kb on - strand, within EX31_RS24060 at 581.885 kb on - strand, within EX31_RS24060 at 581.923 kb on + strand, within EX31_RS24060 at 581.923 kb on + strand, within EX31_RS24060 at 581.924 kb on - strand, within EX31_RS24060 at 581.961 kb on + strand, within EX31_RS24060 at 581.961 kb on + strand, within EX31_RS24060 at 582.001 kb on + strand, within EX31_RS24060 at 582.001 kb on + strand, within EX31_RS24060 at 582.001 kb on + strand, within EX31_RS24060 at 582.001 kb on + strand, within EX31_RS24060 at 582.001 kb on + strand, within EX31_RS24060 at 582.001 kb on + strand, within EX31_RS24060 at 582.002 kb on - strand, within EX31_RS24060 at 582.002 kb on - strand, within EX31_RS24060 at 582.002 kb on - strand, within EX31_RS24060 at 582.002 kb on - strand, within EX31_RS24060 at 582.002 kb on - strand, within EX31_RS24060 at 582.042 kb on - strand, within EX31_RS24060 at 582.203 kb on + strand, within EX31_RS24060 at 582.214 kb on + strand, within EX31_RS24060 at 582.214 kb on + strand, within EX31_RS24060 at 582.214 kb on + strand, within EX31_RS24060 at 582.214 kb on + strand, within EX31_RS24060 at 582.215 kb on - strand, within EX31_RS24060 at 582.215 kb on - strand, within EX31_RS24060 at 582.215 kb on - strand, within EX31_RS24060 at 582.215 kb on - strand, within EX31_RS24060 at 582.215 kb on - strand, within EX31_RS24060 at 582.215 kb on - strand, within EX31_RS24060 at 582.215 kb on - strand, within EX31_RS24060 at 582.249 kb on + strand, within EX31_RS24060 at 582.249 kb on + strand, within EX31_RS24060 at 582.474 kb on - strand, within EX31_RS24060 at 582.474 kb on - strand, within EX31_RS24060 at 582.476 kb on - strand, within EX31_RS24060 at 582.494 kb on + strand, within EX31_RS24060 at 582.494 kb on + strand, within EX31_RS24060 at 582.596 kb on - strand, within EX31_RS24060 at 582.697 kb on - strand, within EX31_RS24060 at 582.697 kb on - strand, within EX31_RS24060 at 582.697 kb on - strand, within EX31_RS24060 at 582.725 kb on + strand at 582.725 kb on + strand at 582.726 kb on - strand at 582.726 kb on - strand at 582.726 kb on - strand at 582.726 kb on - strand at 582.744 kb on + strand at 582.745 kb on - strand at 582.745 kb on - strand at 582.745 kb on - strand at 582.812 kb on + strand at 582.812 kb on + strand at 582.836 kb on + strand at 582.837 kb on - strand at 582.837 kb on - strand at 582.837 kb on - strand at 582.856 kb on + strand at 582.856 kb on + strand at 582.856 kb on + strand at 582.857 kb on - strand at 582.859 kb on + strand at 582.859 kb on + strand at 582.859 kb on + strand at 582.859 kb on + strand at 582.859 kb on + strand at 582.859 kb on + strand at 582.860 kb on - strand at 582.860 kb on - strand at 582.879 kb on - strand at 582.923 kb on + strand at 582.923 kb on + strand at 582.923 kb on + strand at 582.924 kb on - strand at 582.955 kb on + strand, within EX31_RS24065 at 583.162 kb on - strand, within EX31_RS24065 at 583.259 kb on - strand, within EX31_RS24065 at 583.535 kb on + strand at 583.627 kb on - strand, within EX31_RS24070 at 583.733 kb on + strand, within EX31_RS24070 at 583.734 kb on - strand, within EX31_RS24070 at 583.823 kb on + strand, within EX31_RS24070 at 583.823 kb on + strand, within EX31_RS24070 at 583.845 kb on - strand, within EX31_RS24070
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 580,580 + EX31_RS24055 0.60 +2.8 580,580 + EX31_RS24055 0.60 +0.0 580,686 + EX31_RS24055 0.66 -0.3 580,725 + EX31_RS24055 0.68 -3.0 580,726 - EX31_RS24055 0.69 +2.3 580,726 - EX31_RS24055 0.69 -0.1 580,769 + EX31_RS24055 0.71 -2.8 580,769 + EX31_RS24055 0.71 +0.4 580,770 - EX31_RS24055 0.71 -0.1 580,811 + EX31_RS24055 0.74 +0.9 580,811 + EX31_RS24055 0.74 +1.5 580,812 - EX31_RS24055 0.74 +0.2 580,865 + EX31_RS24055 0.77 -0.9 580,865 + EX31_RS24055 0.77 -1.4 580,866 - EX31_RS24055 0.77 -0.6 580,866 - EX31_RS24055 0.77 +0.3 580,869 + EX31_RS24055 0.77 +1.1 580,869 + EX31_RS24055 0.77 +0.0 580,870 - EX31_RS24055 0.77 -0.7 580,870 - EX31_RS24055 0.77 +0.8 580,870 - EX31_RS24055 0.77 +0.4 580,870 - EX31_RS24055 0.77 +1.9 580,949 + EX31_RS24055 0.82 +0.5 580,949 + EX31_RS24055 0.82 +1.4 580,950 - EX31_RS24055 0.82 -0.8 580,959 + EX31_RS24055 0.83 -1.3 580,960 - EX31_RS24055 0.83 +2.7 580,960 - EX31_RS24055 0.83 +0.0 581,024 + EX31_RS24055 0.87 +0.5 581,069 + EX31_RS24055 0.89 -0.7 581,120 + +1.0 581,120 + +0.6 581,245 + -0.9 581,296 + -0.2 581,296 + +1.5 581,296 + -0.3 581,297 - +2.0 581,297 - -0.5 581,297 - +0.5 581,297 - +0.1 581,331 + +0.6 581,331 + +1.3 581,332 - +0.8 581,332 - +0.3 581,477 + +0.4 581,477 + -0.2 581,477 + +0.2 581,478 - -0.1 581,478 - -0.6 581,478 - +0.1 581,478 - -0.5 581,478 - -1.1 581,509 - +0.0 581,788 - EX31_RS24060 0.22 -0.9 581,885 - EX31_RS24060 0.29 -0.4 581,923 + EX31_RS24060 0.32 -0.2 581,923 + EX31_RS24060 0.32 +1.1 581,924 - EX31_RS24060 0.32 +0.5 581,961 + EX31_RS24060 0.34 +0.4 581,961 + EX31_RS24060 0.34 +0.4 582,001 + EX31_RS24060 0.37 +0.8 582,001 + EX31_RS24060 0.37 -0.5 582,001 + EX31_RS24060 0.37 +0.3 582,001 + EX31_RS24060 0.37 -0.3 582,001 + EX31_RS24060 0.37 -0.3 582,001 + EX31_RS24060 0.37 -1.2 582,002 - EX31_RS24060 0.37 -0.2 582,002 - EX31_RS24060 0.37 +0.5 582,002 - EX31_RS24060 0.37 +0.9 582,002 - EX31_RS24060 0.37 +1.7 582,002 - EX31_RS24060 0.37 -1.2 582,042 - EX31_RS24060 0.40 -0.8 582,203 + EX31_RS24060 0.52 -0.8 582,214 + EX31_RS24060 0.53 +0.2 582,214 + EX31_RS24060 0.53 +0.4 582,214 + EX31_RS24060 0.53 -1.1 582,214 + EX31_RS24060 0.53 +1.0 582,215 - EX31_RS24060 0.53 -0.8 582,215 - EX31_RS24060 0.53 +0.2 582,215 - EX31_RS24060 0.53 -0.3 582,215 - EX31_RS24060 0.53 +0.8 582,215 - EX31_RS24060 0.53 -1.0 582,215 - EX31_RS24060 0.53 -0.2 582,215 - EX31_RS24060 0.53 -0.5 582,249 + EX31_RS24060 0.56 -2.2 582,249 + EX31_RS24060 0.56 +0.0 582,474 - EX31_RS24060 0.72 +0.6 582,474 - EX31_RS24060 0.72 +1.0 582,476 - EX31_RS24060 0.73 -0.5 582,494 + EX31_RS24060 0.74 -0.2 582,494 + EX31_RS24060 0.74 +0.8 582,596 - EX31_RS24060 0.81 +0.0 582,697 - EX31_RS24060 0.89 -1.2 582,697 - EX31_RS24060 0.89 +0.4 582,697 - EX31_RS24060 0.89 -0.4 582,725 + -0.6 582,725 + +0.9 582,726 - +1.0 582,726 - +1.1 582,726 - +0.1 582,726 - +0.9 582,744 + -0.1 582,745 - +1.2 582,745 - -0.3 582,745 - -2.7 582,812 + -0.2 582,812 + -0.9 582,836 + +0.5 582,837 - -1.2 582,837 - +1.6 582,837 - +0.0 582,856 + -0.0 582,856 + +1.0 582,856 + +0.9 582,857 - +0.0 582,859 + +0.1 582,859 + +0.1 582,859 + +0.8 582,859 + +1.3 582,859 + +0.5 582,859 + -0.9 582,860 - +0.3 582,860 - +1.0 582,879 - -1.4 582,923 + +0.3 582,923 + +0.3 582,923 + -1.6 582,924 - +1.1 582,955 + EX31_RS24065 0.12 -1.4 583,162 - EX31_RS24065 0.45 +0.2 583,259 - EX31_RS24065 0.61 +0.7 583,535 + -0.5 583,627 - EX31_RS24070 0.16 +1.1 583,733 + EX31_RS24070 0.34 +0.1 583,734 - EX31_RS24070 0.34 +0.1 583,823 + EX31_RS24070 0.49 -0.9 583,823 + EX31_RS24070 0.49 +0.5 583,845 - EX31_RS24070 0.53 -1.4
Or see this region's nucleotide sequence