Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23195 and EX31_RS23205 are separated by 245 nucleotides EX31_RS23205 and EX31_RS23210 are separated by 188 nucleotides
EX31_RS23195: EX31_RS23195 - PhoH family protein, at 400,980 to 402,056
_RS23195
EX31_RS23205: EX31_RS23205 - tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB, at 402,302 to 403,726
_RS23205
EX31_RS23210: EX31_RS23210 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 403,915 to 405,099
_RS23210
Position (kb) 402
403
404 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 401.322 kb on - strand, within EX31_RS23195 at 401.325 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.343 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.357 kb on - strand, within EX31_RS23195 at 401.441 kb on - strand, within EX31_RS23195 at 401.441 kb on - strand, within EX31_RS23195 at 401.456 kb on - strand, within EX31_RS23195 at 401.591 kb on - strand, within EX31_RS23195 at 401.591 kb on - strand, within EX31_RS23195 at 401.591 kb on - strand, within EX31_RS23195 at 401.628 kb on - strand, within EX31_RS23195 at 401.628 kb on - strand, within EX31_RS23195 at 401.628 kb on - strand, within EX31_RS23195 at 401.655 kb on - strand, within EX31_RS23195 at 401.655 kb on - strand, within EX31_RS23195 at 401.657 kb on - strand, within EX31_RS23195 at 401.657 kb on - strand, within EX31_RS23195 at 401.657 kb on - strand, within EX31_RS23195 at 401.840 kb on - strand, within EX31_RS23195 at 401.842 kb on - strand, within EX31_RS23195 at 401.955 kb on - strand at 401.955 kb on - strand at 401.955 kb on - strand at 402.121 kb on - strand at 402.121 kb on - strand at 402.121 kb on - strand at 402.121 kb on - strand at 402.124 kb on - strand at 402.126 kb on + strand at 402.126 kb on + strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.127 kb on - strand at 402.145 kb on - strand at 402.145 kb on - strand at 402.216 kb on + strand at 402.216 kb on + strand at 402.216 kb on + strand at 402.217 kb on - strand at 402.227 kb on + strand at 402.227 kb on + strand at 402.228 kb on - strand at 402.228 kb on - strand at 402.228 kb on - strand at 402.228 kb on - strand at 402.228 kb on - strand at 402.312 kb on + strand at 402.312 kb on + strand at 402.313 kb on - strand at 402.313 kb on - strand at 402.314 kb on + strand at 402.314 kb on + strand at 402.314 kb on + strand at 402.315 kb on - strand at 402.315 kb on - strand at 402.315 kb on - strand at 402.315 kb on - strand at 402.315 kb on - strand at 402.435 kb on + strand at 402.436 kb on - strand at 402.436 kb on - strand at 402.436 kb on - strand at 402.436 kb on - strand at 402.467 kb on - strand, within EX31_RS23205 at 402.569 kb on - strand, within EX31_RS23205 at 402.581 kb on - strand, within EX31_RS23205 at 402.682 kb on + strand, within EX31_RS23205 at 402.683 kb on - strand, within EX31_RS23205 at 402.683 kb on - strand, within EX31_RS23205 at 402.683 kb on - strand, within EX31_RS23205 at 402.683 kb on - strand, within EX31_RS23205 at 402.714 kb on + strand, within EX31_RS23205 at 402.766 kb on + strand, within EX31_RS23205 at 402.766 kb on + strand, within EX31_RS23205 at 402.766 kb on + strand, within EX31_RS23205 at 402.766 kb on + strand, within EX31_RS23205 at 402.766 kb on + strand, within EX31_RS23205 at 402.766 kb on + strand, within EX31_RS23205 at 402.766 kb on + strand, within EX31_RS23205 at 402.767 kb on - strand, within EX31_RS23205 at 402.767 kb on - strand, within EX31_RS23205 at 402.767 kb on - strand, within EX31_RS23205 at 402.767 kb on - strand, within EX31_RS23205 at 402.767 kb on - strand, within EX31_RS23205 at 402.808 kb on + strand, within EX31_RS23205 at 402.830 kb on - strand, within EX31_RS23205 at 402.830 kb on - strand, within EX31_RS23205 at 402.849 kb on - strand, within EX31_RS23205 at 402.849 kb on - strand, within EX31_RS23205 at 402.869 kb on - strand, within EX31_RS23205 at 402.869 kb on - strand, within EX31_RS23205 at 402.870 kb on + strand, within EX31_RS23205 at 402.871 kb on - strand, within EX31_RS23205 at 402.871 kb on - strand, within EX31_RS23205 at 402.883 kb on + strand, within EX31_RS23205 at 402.883 kb on + strand, within EX31_RS23205 at 402.884 kb on - strand, within EX31_RS23205 at 402.884 kb on - strand, within EX31_RS23205 at 402.884 kb on - strand, within EX31_RS23205 at 402.884 kb on - strand, within EX31_RS23205 at 402.964 kb on + strand, within EX31_RS23205 at 402.965 kb on - strand, within EX31_RS23205 at 402.970 kb on + strand, within EX31_RS23205 at 402.972 kb on + strand, within EX31_RS23205 at 402.973 kb on - strand, within EX31_RS23205 at 402.973 kb on - strand, within EX31_RS23205 at 402.980 kb on + strand, within EX31_RS23205 at 402.980 kb on + strand, within EX31_RS23205 at 402.980 kb on + strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.981 kb on - strand, within EX31_RS23205 at 402.983 kb on - strand, within EX31_RS23205 at 402.983 kb on - strand, within EX31_RS23205 at 402.983 kb on - strand, within EX31_RS23205 at 402.985 kb on + strand, within EX31_RS23205 at 402.985 kb on + strand, within EX31_RS23205 at 402.985 kb on + strand, within EX31_RS23205 at 402.985 kb on + strand, within EX31_RS23205 at 402.986 kb on - strand, within EX31_RS23205 at 403.184 kb on + strand, within EX31_RS23205 at 403.184 kb on + strand, within EX31_RS23205 at 403.185 kb on - strand, within EX31_RS23205 at 403.185 kb on - strand, within EX31_RS23205 at 403.185 kb on - strand, within EX31_RS23205 at 403.185 kb on - strand, within EX31_RS23205 at 403.185 kb on - strand, within EX31_RS23205 at 403.185 kb on - strand, within EX31_RS23205 at 403.185 kb on - strand, within EX31_RS23205 at 403.222 kb on + strand, within EX31_RS23205 at 403.222 kb on + strand, within EX31_RS23205 at 403.222 kb on + strand, within EX31_RS23205 at 403.223 kb on - strand, within EX31_RS23205 at 403.223 kb on - strand, within EX31_RS23205 at 403.223 kb on - strand, within EX31_RS23205 at 403.223 kb on - strand, within EX31_RS23205 at 403.223 kb on - strand, within EX31_RS23205 at 403.223 kb on - strand, within EX31_RS23205 at 403.225 kb on - strand, within EX31_RS23205 at 403.233 kb on - strand, within EX31_RS23205 at 403.233 kb on - strand, within EX31_RS23205 at 403.248 kb on + strand, within EX31_RS23205 at 403.248 kb on + strand, within EX31_RS23205 at 403.249 kb on - strand, within EX31_RS23205 at 403.249 kb on - strand, within EX31_RS23205 at 403.373 kb on + strand, within EX31_RS23205 at 403.373 kb on + strand, within EX31_RS23205 at 403.373 kb on + strand, within EX31_RS23205 at 403.374 kb on - strand, within EX31_RS23205 at 403.374 kb on - strand, within EX31_RS23205 at 403.374 kb on - strand, within EX31_RS23205 at 403.374 kb on - strand, within EX31_RS23205 at 403.586 kb on - strand at 403.586 kb on - strand at 403.677 kb on + strand at 403.678 kb on - strand at 403.678 kb on - strand at 403.706 kb on + strand at 403.707 kb on - strand at 403.707 kb on - strand at 403.707 kb on - strand at 403.738 kb on + strand at 403.738 kb on + strand at 403.739 kb on - strand at 403.739 kb on - strand at 403.757 kb on + strand at 403.757 kb on + strand at 403.757 kb on + strand at 403.757 kb on + strand at 403.758 kb on - strand at 403.780 kb on + strand at 403.780 kb on + strand at 403.780 kb on + strand at 403.780 kb on + strand at 403.780 kb on + strand at 403.781 kb on - strand at 403.781 kb on - strand at 403.781 kb on - strand at 403.781 kb on - strand at 403.781 kb on - strand at 403.781 kb on - strand at 403.781 kb on - strand at 403.785 kb on + strand at 403.785 kb on + strand at 403.785 kb on + strand at 403.785 kb on + strand at 403.785 kb on + strand at 403.785 kb on + strand at 403.785 kb on + strand at 403.785 kb on + strand at 403.785 kb on + strand at 403.785 kb on + strand at 403.786 kb on - strand at 403.786 kb on - strand at 403.786 kb on - strand at 403.786 kb on - strand at 403.786 kb on - strand at 403.786 kb on - strand at 403.786 kb on - strand at 403.786 kb on - strand at 403.786 kb on - strand at 403.786 kb on - strand at 403.786 kb on - strand at 403.786 kb on - strand at 403.786 kb on - strand at 403.804 kb on + strand at 403.845 kb on + strand at 403.845 kb on + strand at 403.845 kb on + strand at 403.845 kb on + strand at 403.845 kb on + strand at 403.846 kb on - strand at 403.846 kb on - strand at 403.874 kb on + strand at 403.910 kb on + strand at 403.913 kb on + strand at 403.914 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 401,322 - EX31_RS23195 0.32 +1.0 401,325 - EX31_RS23195 0.32 +0.4 401,343 - EX31_RS23195 0.34 -0.7 401,343 - EX31_RS23195 0.34 -0.0 401,343 - EX31_RS23195 0.34 +0.7 401,343 - EX31_RS23195 0.34 +0.3 401,343 - EX31_RS23195 0.34 -0.7 401,343 - EX31_RS23195 0.34 -0.7 401,343 - EX31_RS23195 0.34 -0.5 401,343 - EX31_RS23195 0.34 +0.0 401,343 - EX31_RS23195 0.34 +1.4 401,343 - EX31_RS23195 0.34 +1.2 401,357 - EX31_RS23195 0.35 +0.9 401,357 - EX31_RS23195 0.35 -0.2 401,357 - EX31_RS23195 0.35 +1.0 401,357 - EX31_RS23195 0.35 +0.2 401,357 - EX31_RS23195 0.35 -0.3 401,357 - EX31_RS23195 0.35 +0.8 401,357 - EX31_RS23195 0.35 -0.3 401,357 - EX31_RS23195 0.35 +0.5 401,357 - EX31_RS23195 0.35 -1.3 401,357 - EX31_RS23195 0.35 -1.4 401,357 - EX31_RS23195 0.35 -0.6 401,357 - EX31_RS23195 0.35 -0.4 401,441 - EX31_RS23195 0.43 -1.1 401,441 - EX31_RS23195 0.43 -0.3 401,456 - EX31_RS23195 0.44 -0.5 401,591 - EX31_RS23195 0.57 +1.5 401,591 - EX31_RS23195 0.57 -0.8 401,591 - EX31_RS23195 0.57 -0.5 401,628 - EX31_RS23195 0.60 +0.2 401,628 - EX31_RS23195 0.60 -1.0 401,628 - EX31_RS23195 0.60 +0.5 401,655 - EX31_RS23195 0.63 -0.5 401,655 - EX31_RS23195 0.63 +0.0 401,657 - EX31_RS23195 0.63 +0.2 401,657 - EX31_RS23195 0.63 +0.0 401,657 - EX31_RS23195 0.63 +0.6 401,840 - EX31_RS23195 0.80 -0.4 401,842 - EX31_RS23195 0.80 -0.1 401,955 - +0.2 401,955 - +0.5 401,955 - +1.4 402,121 - +0.3 402,121 - -0.5 402,121 - +0.3 402,121 - +0.0 402,124 - -1.1 402,126 + -0.1 402,126 + +2.8 402,127 - +0.3 402,127 - -2.2 402,127 - +0.6 402,127 - +1.8 402,127 - +1.4 402,127 - -0.7 402,127 - +0.2 402,127 - -0.6 402,127 - +0.7 402,127 - -0.6 402,127 - +0.2 402,127 - -0.4 402,127 - +0.3 402,127 - +0.1 402,127 - -1.0 402,127 - -1.6 402,127 - -0.8 402,127 - +0.4 402,127 - -2.9 402,127 - -0.9 402,127 - -1.6 402,127 - -0.3 402,145 - -0.9 402,145 - +0.5 402,216 + -0.7 402,216 + -0.4 402,216 + -1.4 402,217 - -2.1 402,227 + -0.0 402,227 + +0.0 402,228 - +0.6 402,228 - -0.5 402,228 - -1.4 402,228 - -0.3 402,228 - +0.1 402,312 + -0.5 402,312 + -1.2 402,313 - -1.1 402,313 - -1.3 402,314 + -1.3 402,314 + +0.6 402,314 + -0.8 402,315 - -1.5 402,315 - -2.0 402,315 - -0.1 402,315 - +0.4 402,315 - -0.8 402,435 + +0.1 402,436 - -0.9 402,436 - +1.0 402,436 - -1.6 402,436 - +0.5 402,467 - EX31_RS23205 0.12 +0.0 402,569 - EX31_RS23205 0.19 -1.5 402,581 - EX31_RS23205 0.20 -0.3 402,682 + EX31_RS23205 0.27 -0.3 402,683 - EX31_RS23205 0.27 -1.1 402,683 - EX31_RS23205 0.27 +0.5 402,683 - EX31_RS23205 0.27 +0.2 402,683 - EX31_RS23205 0.27 -0.1 402,714 + EX31_RS23205 0.29 +1.0 402,766 + EX31_RS23205 0.33 +0.5 402,766 + EX31_RS23205 0.33 -0.4 402,766 + EX31_RS23205 0.33 -0.3 402,766 + EX31_RS23205 0.33 -2.0 402,766 + EX31_RS23205 0.33 -0.3 402,766 + EX31_RS23205 0.33 +0.5 402,766 + EX31_RS23205 0.33 +1.0 402,767 - EX31_RS23205 0.33 +0.2 402,767 - EX31_RS23205 0.33 -1.5 402,767 - EX31_RS23205 0.33 -1.6 402,767 - EX31_RS23205 0.33 -0.5 402,767 - EX31_RS23205 0.33 -0.5 402,808 + EX31_RS23205 0.36 +0.0 402,830 - EX31_RS23205 0.37 +0.4 402,830 - EX31_RS23205 0.37 -0.3 402,849 - EX31_RS23205 0.38 +0.0 402,849 - EX31_RS23205 0.38 +1.0 402,869 - EX31_RS23205 0.40 -0.5 402,869 - EX31_RS23205 0.40 +0.7 402,870 + EX31_RS23205 0.40 +0.8 402,871 - EX31_RS23205 0.40 -2.0 402,871 - EX31_RS23205 0.40 -0.5 402,883 + EX31_RS23205 0.41 -0.1 402,883 + EX31_RS23205 0.41 -1.2 402,884 - EX31_RS23205 0.41 -2.3 402,884 - EX31_RS23205 0.41 +0.0 402,884 - EX31_RS23205 0.41 -0.0 402,884 - EX31_RS23205 0.41 -1.2 402,964 + EX31_RS23205 0.46 -0.5 402,965 - EX31_RS23205 0.47 +0.5 402,970 + EX31_RS23205 0.47 +0.3 402,972 + EX31_RS23205 0.47 +2.0 402,973 - EX31_RS23205 0.47 +0.4 402,973 - EX31_RS23205 0.47 +0.8 402,980 + EX31_RS23205 0.48 +1.0 402,980 + EX31_RS23205 0.48 -2.3 402,980 + EX31_RS23205 0.48 +1.5 402,981 - EX31_RS23205 0.48 -1.7 402,981 - EX31_RS23205 0.48 +0.0 402,981 - EX31_RS23205 0.48 -1.0 402,981 - EX31_RS23205 0.48 -0.5 402,981 - EX31_RS23205 0.48 +0.0 402,981 - EX31_RS23205 0.48 -1.0 402,981 - EX31_RS23205 0.48 +0.3 402,981 - EX31_RS23205 0.48 -0.3 402,981 - EX31_RS23205 0.48 -0.8 402,983 - EX31_RS23205 0.48 +1.9 402,983 - EX31_RS23205 0.48 -1.5 402,983 - EX31_RS23205 0.48 -0.2 402,985 + EX31_RS23205 0.48 +1.3 402,985 + EX31_RS23205 0.48 +0.3 402,985 + EX31_RS23205 0.48 +0.8 402,985 + EX31_RS23205 0.48 +1.2 402,986 - EX31_RS23205 0.48 -0.4 403,184 + EX31_RS23205 0.62 -0.6 403,184 + EX31_RS23205 0.62 +1.3 403,185 - EX31_RS23205 0.62 -0.3 403,185 - EX31_RS23205 0.62 -0.7 403,185 - EX31_RS23205 0.62 -0.4 403,185 - EX31_RS23205 0.62 +0.4 403,185 - EX31_RS23205 0.62 -1.2 403,185 - EX31_RS23205 0.62 -0.1 403,185 - EX31_RS23205 0.62 +0.6 403,222 + EX31_RS23205 0.65 +0.5 403,222 + EX31_RS23205 0.65 +3.0 403,222 + EX31_RS23205 0.65 -1.2 403,223 - EX31_RS23205 0.65 -1.6 403,223 - EX31_RS23205 0.65 +0.2 403,223 - EX31_RS23205 0.65 -0.7 403,223 - EX31_RS23205 0.65 +2.2 403,223 - EX31_RS23205 0.65 +0.0 403,223 - EX31_RS23205 0.65 -1.0 403,225 - EX31_RS23205 0.65 +0.0 403,233 - EX31_RS23205 0.65 -0.6 403,233 - EX31_RS23205 0.65 -0.3 403,248 + EX31_RS23205 0.66 -0.8 403,248 + EX31_RS23205 0.66 -0.3 403,249 - EX31_RS23205 0.66 -0.1 403,249 - EX31_RS23205 0.66 -0.4 403,373 + EX31_RS23205 0.75 -1.4 403,373 + EX31_RS23205 0.75 +1.3 403,373 + EX31_RS23205 0.75 -0.1 403,374 - EX31_RS23205 0.75 +0.8 403,374 - EX31_RS23205 0.75 +0.9 403,374 - EX31_RS23205 0.75 -1.0 403,374 - EX31_RS23205 0.75 -2.2 403,586 - +0.5 403,586 - -0.1 403,677 + -1.7 403,678 - +0.3 403,678 - -1.9 403,706 + +2.0 403,707 - +0.2 403,707 - +2.4 403,707 - +1.2 403,738 + +0.3 403,738 + +0.3 403,739 - -0.5 403,739 - +0.6 403,757 + -0.4 403,757 + +0.8 403,757 + -1.5 403,757 + -0.4 403,758 - -0.0 403,780 + +0.1 403,780 + -0.4 403,780 + -1.3 403,780 + -0.3 403,780 + -1.1 403,781 - +0.0 403,781 - -1.0 403,781 - +0.0 403,781 - -0.6 403,781 - +1.2 403,781 - +0.4 403,781 - -0.6 403,785 + +0.0 403,785 + +0.3 403,785 + -1.0 403,785 + +1.2 403,785 + +1.6 403,785 + -0.1 403,785 + -0.5 403,785 + +0.8 403,785 + -1.0 403,785 + +0.0 403,786 - -1.0 403,786 - +0.7 403,786 - +0.1 403,786 - -0.7 403,786 - +0.5 403,786 - +0.0 403,786 - +0.7 403,786 - +0.5 403,786 - +0.7 403,786 - +0.3 403,786 - +0.5 403,786 - -0.5 403,786 - +0.5 403,804 + -0.3 403,845 + -0.8 403,845 + +0.5 403,845 + -1.3 403,845 + +1.3 403,845 + -0.0 403,846 - +0.5 403,846 - +0.0 403,874 + +0.6 403,910 + -1.7 403,913 + +0.1 403,914 - +0.5
Or see this region's nucleotide sequence