Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20815 and EX31_RS20820 are separated by 206 nucleotides EX31_RS20820 and EX31_RS20825 are separated by 26 nucleotides
EX31_RS20815: EX31_RS20815 - hypothetical protein, at 812,824 to 813,093
_RS20815
EX31_RS20820: EX31_RS20820 - aldo/keto reductase, at 813,300 to 814,151
_RS20820
EX31_RS20825: EX31_RS20825 - aldo/keto reductase, at 814,178 to 815,167
_RS20825
Position (kb) 813
814
815 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 812.329 kb on + strand at 812.345 kb on + strand at 812.348 kb on + strand at 812.367 kb on - strand at 812.371 kb on - strand at 812.373 kb on + strand at 812.373 kb on + strand at 812.373 kb on + strand at 812.374 kb on - strand at 812.374 kb on - strand at 812.474 kb on + strand at 812.475 kb on - strand at 812.484 kb on - strand at 812.497 kb on + strand at 812.521 kb on - strand at 812.551 kb on + strand at 812.551 kb on + strand at 812.552 kb on - strand at 812.552 kb on - strand at 812.561 kb on - strand at 812.561 kb on - strand at 812.576 kb on - strand at 812.576 kb on - strand at 812.576 kb on - strand at 812.584 kb on - strand at 812.601 kb on + strand at 812.605 kb on + strand at 812.605 kb on + strand at 812.606 kb on - strand at 812.627 kb on + strand at 812.627 kb on + strand at 812.628 kb on - strand at 812.628 kb on - strand at 812.628 kb on - strand at 812.628 kb on - strand at 812.684 kb on + strand at 812.684 kb on + strand at 812.684 kb on + strand at 812.684 kb on + strand at 812.685 kb on - strand at 812.783 kb on + strand at 812.783 kb on + strand at 812.785 kb on + strand at 812.785 kb on + strand at 812.785 kb on + strand at 812.785 kb on + strand at 812.785 kb on + strand at 812.785 kb on + strand at 812.785 kb on + strand at 812.785 kb on + strand at 812.786 kb on - strand at 812.786 kb on - strand at 812.786 kb on - strand at 812.786 kb on - strand at 812.786 kb on - strand at 812.793 kb on + strand at 812.798 kb on - strand at 812.994 kb on + strand, within EX31_RS20815 at 813.002 kb on + strand, within EX31_RS20815 at 813.002 kb on + strand, within EX31_RS20815 at 813.002 kb on + strand, within EX31_RS20815 at 813.003 kb on - strand, within EX31_RS20815 at 813.003 kb on - strand, within EX31_RS20815 at 813.003 kb on - strand, within EX31_RS20815 at 813.003 kb on - strand, within EX31_RS20815 at 813.003 kb on - strand, within EX31_RS20815 at 813.003 kb on - strand, within EX31_RS20815 at 813.003 kb on - strand, within EX31_RS20815 at 813.036 kb on + strand, within EX31_RS20815 at 813.037 kb on - strand, within EX31_RS20815 at 813.037 kb on - strand, within EX31_RS20815 at 813.045 kb on + strand, within EX31_RS20815 at 813.045 kb on + strand, within EX31_RS20815 at 813.045 kb on + strand, within EX31_RS20815 at 813.045 kb on + strand, within EX31_RS20815 at 813.045 kb on + strand, within EX31_RS20815 at 813.045 kb on + strand, within EX31_RS20815 at 813.046 kb on - strand, within EX31_RS20815 at 813.046 kb on - strand, within EX31_RS20815 at 813.046 kb on - strand, within EX31_RS20815 at 813.046 kb on - strand, within EX31_RS20815 at 813.046 kb on - strand, within EX31_RS20815 at 813.047 kb on + strand, within EX31_RS20815 at 813.047 kb on + strand, within EX31_RS20815 at 813.047 kb on + strand, within EX31_RS20815 at 813.047 kb on + strand, within EX31_RS20815 at 813.047 kb on + strand, within EX31_RS20815 at 813.047 kb on + strand, within EX31_RS20815 at 813.047 kb on + strand, within EX31_RS20815 at 813.047 kb on + strand, within EX31_RS20815 at 813.048 kb on - strand, within EX31_RS20815 at 813.048 kb on - strand, within EX31_RS20815 at 813.048 kb on - strand, within EX31_RS20815 at 813.048 kb on - strand, within EX31_RS20815 at 813.048 kb on - strand, within EX31_RS20815 at 813.069 kb on + strand at 813.069 kb on + strand at 813.070 kb on - strand at 813.070 kb on - strand at 813.070 kb on - strand at 813.070 kb on - strand at 813.073 kb on + strand at 813.074 kb on - strand at 813.074 kb on - strand at 813.074 kb on - strand at 813.074 kb on - strand at 813.074 kb on - strand at 813.074 kb on - strand at 813.074 kb on - strand at 813.074 kb on - strand at 813.074 kb on - strand at 813.091 kb on + strand at 813.101 kb on - strand at 813.132 kb on + strand at 813.133 kb on - strand at 813.143 kb on + strand at 813.143 kb on + strand at 813.143 kb on + strand at 813.146 kb on - strand at 813.156 kb on + strand at 813.156 kb on + strand at 813.156 kb on + strand at 813.156 kb on + strand at 813.156 kb on + strand at 813.156 kb on + strand at 813.156 kb on + strand at 813.156 kb on + strand at 813.157 kb on - strand at 813.157 kb on - strand at 813.157 kb on - strand at 813.157 kb on - strand at 813.275 kb on + strand at 813.275 kb on + strand at 813.276 kb on - strand at 813.276 kb on - strand at 813.294 kb on + strand at 813.294 kb on + strand at 813.294 kb on + strand at 813.294 kb on + strand at 813.295 kb on - strand at 813.295 kb on - strand at 813.295 kb on - strand at 813.295 kb on - strand at 813.295 kb on - strand at 813.295 kb on - strand at 813.295 kb on - strand at 813.295 kb on - strand at 813.295 kb on - strand at 813.295 kb on - strand at 813.311 kb on - strand at 813.311 kb on - strand at 813.445 kb on - strand, within EX31_RS20820 at 813.511 kb on + strand, within EX31_RS20820 at 813.511 kb on + strand, within EX31_RS20820 at 813.511 kb on + strand, within EX31_RS20820 at 813.512 kb on - strand, within EX31_RS20820 at 813.512 kb on - strand, within EX31_RS20820 at 813.512 kb on - strand, within EX31_RS20820 at 813.512 kb on - strand, within EX31_RS20820 at 813.536 kb on + strand, within EX31_RS20820 at 813.543 kb on + strand, within EX31_RS20820 at 813.543 kb on + strand, within EX31_RS20820 at 813.543 kb on + strand, within EX31_RS20820 at 813.554 kb on + strand, within EX31_RS20820 at 813.554 kb on + strand, within EX31_RS20820 at 813.554 kb on + strand, within EX31_RS20820 at 813.555 kb on - strand, within EX31_RS20820 at 813.555 kb on - strand, within EX31_RS20820 at 813.597 kb on + strand, within EX31_RS20820 at 813.597 kb on + strand, within EX31_RS20820 at 813.597 kb on + strand, within EX31_RS20820 at 813.597 kb on + strand, within EX31_RS20820 at 813.597 kb on + strand, within EX31_RS20820 at 813.609 kb on + strand, within EX31_RS20820 at 813.609 kb on + strand, within EX31_RS20820 at 813.610 kb on - strand, within EX31_RS20820 at 813.610 kb on - strand, within EX31_RS20820 at 813.627 kb on + strand, within EX31_RS20820 at 813.866 kb on + strand, within EX31_RS20820 at 813.867 kb on - strand, within EX31_RS20820 at 813.867 kb on - strand, within EX31_RS20820 at 813.915 kb on + strand, within EX31_RS20820 at 813.916 kb on - strand, within EX31_RS20820 at 813.916 kb on - strand, within EX31_RS20820 at 814.001 kb on + strand, within EX31_RS20820 at 814.001 kb on + strand, within EX31_RS20820 at 814.002 kb on - strand, within EX31_RS20820 at 814.002 kb on - strand, within EX31_RS20820 at 814.002 kb on - strand, within EX31_RS20820 at 814.049 kb on + strand, within EX31_RS20820 at 814.049 kb on + strand, within EX31_RS20820 at 814.049 kb on + strand, within EX31_RS20820 at 814.049 kb on + strand, within EX31_RS20820 at 814.049 kb on + strand, within EX31_RS20820 at 814.049 kb on + strand, within EX31_RS20820 at 814.049 kb on + strand, within EX31_RS20820 at 814.049 kb on + strand, within EX31_RS20820 at 814.049 kb on + strand, within EX31_RS20820 at 814.049 kb on + strand, within EX31_RS20820 at 814.049 kb on + strand, within EX31_RS20820 at 814.049 kb on + strand, within EX31_RS20820 at 814.050 kb on - strand, within EX31_RS20820 at 814.050 kb on - strand, within EX31_RS20820 at 814.050 kb on - strand, within EX31_RS20820 at 814.050 kb on - strand, within EX31_RS20820 at 814.050 kb on - strand, within EX31_RS20820 at 814.050 kb on - strand, within EX31_RS20820 at 814.050 kb on - strand, within EX31_RS20820 at 814.149 kb on + strand at 814.149 kb on + strand at 814.150 kb on - strand at 814.168 kb on + strand at 814.168 kb on + strand at 814.169 kb on - strand at 814.190 kb on + strand at 814.273 kb on + strand at 814.346 kb on + strand, within EX31_RS20825 at 814.346 kb on + strand, within EX31_RS20825 at 814.346 kb on + strand, within EX31_RS20825 at 814.346 kb on + strand, within EX31_RS20825 at 814.346 kb on + strand, within EX31_RS20825 at 814.346 kb on + strand, within EX31_RS20825 at 814.347 kb on - strand, within EX31_RS20825 at 814.347 kb on - strand, within EX31_RS20825 at 814.347 kb on - strand, within EX31_RS20825 at 814.386 kb on + strand, within EX31_RS20825 at 814.433 kb on - strand, within EX31_RS20825 at 814.509 kb on + strand, within EX31_RS20825 at 814.509 kb on + strand, within EX31_RS20825 at 814.509 kb on + strand, within EX31_RS20825 at 814.510 kb on - strand, within EX31_RS20825 at 814.510 kb on - strand, within EX31_RS20825 at 814.520 kb on - strand, within EX31_RS20825 at 814.531 kb on + strand, within EX31_RS20825 at 814.531 kb on + strand, within EX31_RS20825 at 814.532 kb on - strand, within EX31_RS20825 at 814.532 kb on - strand, within EX31_RS20825 at 814.532 kb on - strand, within EX31_RS20825 at 814.544 kb on + strand, within EX31_RS20825 at 814.608 kb on + strand, within EX31_RS20825 at 814.609 kb on - strand, within EX31_RS20825 at 814.624 kb on + strand, within EX31_RS20825 at 814.624 kb on + strand, within EX31_RS20825 at 814.681 kb on - strand, within EX31_RS20825 at 814.681 kb on - strand, within EX31_RS20825 at 814.681 kb on - strand, within EX31_RS20825 at 814.681 kb on - strand, within EX31_RS20825 at 814.799 kb on - strand, within EX31_RS20825 at 814.853 kb on + strand, within EX31_RS20825 at 815.132 kb on + strand at 815.132 kb on + strand at 815.133 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 812,329 + +0.6 812,345 + -1.3 812,348 + -0.0 812,367 - -2.0 812,371 - +0.2 812,373 + +0.1 812,373 + +0.8 812,373 + +1.1 812,374 - +0.5 812,374 - +0.2 812,474 + -0.1 812,475 - -0.3 812,484 - -2.5 812,497 + -1.8 812,521 - -1.4 812,551 + -0.7 812,551 + +0.1 812,552 - -1.2 812,552 - -0.1 812,561 - +0.4 812,561 - -0.1 812,576 - -0.7 812,576 - +0.5 812,576 - -1.0 812,584 - +1.3 812,601 + +0.5 812,605 + -1.3 812,605 + +0.2 812,606 - +1.1 812,627 + -0.3 812,627 + +0.3 812,628 - +0.7 812,628 - -0.3 812,628 - +0.0 812,628 - -0.1 812,684 + -0.1 812,684 + -0.1 812,684 + +0.1 812,684 + +0.7 812,685 - +0.8 812,783 + -0.1 812,783 + +0.0 812,785 + +1.3 812,785 + +1.8 812,785 + -1.3 812,785 + +0.0 812,785 + +0.1 812,785 + +0.6 812,785 + -0.4 812,785 + -0.7 812,786 - -0.8 812,786 - -0.5 812,786 - -0.5 812,786 - +0.1 812,786 - +1.4 812,793 + +1.5 812,798 - -0.2 812,994 + EX31_RS20815 0.63 -1.8 813,002 + EX31_RS20815 0.66 +0.0 813,002 + EX31_RS20815 0.66 -0.7 813,002 + EX31_RS20815 0.66 +0.7 813,003 - EX31_RS20815 0.66 +0.7 813,003 - EX31_RS20815 0.66 +0.5 813,003 - EX31_RS20815 0.66 -0.1 813,003 - EX31_RS20815 0.66 -0.5 813,003 - EX31_RS20815 0.66 +1.1 813,003 - EX31_RS20815 0.66 -0.0 813,003 - EX31_RS20815 0.66 -1.0 813,036 + EX31_RS20815 0.79 +0.3 813,037 - EX31_RS20815 0.79 +0.6 813,037 - EX31_RS20815 0.79 +0.0 813,045 + EX31_RS20815 0.82 -0.2 813,045 + EX31_RS20815 0.82 -1.2 813,045 + EX31_RS20815 0.82 +1.0 813,045 + EX31_RS20815 0.82 +0.7 813,045 + EX31_RS20815 0.82 -1.5 813,045 + EX31_RS20815 0.82 +1.2 813,046 - EX31_RS20815 0.82 -0.7 813,046 - EX31_RS20815 0.82 -0.1 813,046 - EX31_RS20815 0.82 +0.7 813,046 - EX31_RS20815 0.82 +0.6 813,046 - EX31_RS20815 0.82 -1.8 813,047 + EX31_RS20815 0.83 +0.5 813,047 + EX31_RS20815 0.83 +1.2 813,047 + EX31_RS20815 0.83 -0.5 813,047 + EX31_RS20815 0.83 -0.1 813,047 + EX31_RS20815 0.83 +1.6 813,047 + EX31_RS20815 0.83 -0.3 813,047 + EX31_RS20815 0.83 -0.5 813,047 + EX31_RS20815 0.83 -0.1 813,048 - EX31_RS20815 0.83 +0.4 813,048 - EX31_RS20815 0.83 +3.0 813,048 - EX31_RS20815 0.83 -0.8 813,048 - EX31_RS20815 0.83 +0.2 813,048 - EX31_RS20815 0.83 +0.6 813,069 + +0.3 813,069 + +1.3 813,070 - +0.9 813,070 - +0.9 813,070 - +0.6 813,070 - -0.1 813,073 + +1.0 813,074 - +0.7 813,074 - +1.3 813,074 - -0.5 813,074 - +1.0 813,074 - -0.1 813,074 - +0.5 813,074 - +0.9 813,074 - -0.4 813,074 - +0.5 813,091 + -1.0 813,101 - -0.3 813,132 + -0.6 813,133 - +1.3 813,143 + +1.0 813,143 + -0.3 813,143 + +0.5 813,146 - +0.8 813,156 + -0.7 813,156 + -1.9 813,156 + +1.0 813,156 + +0.1 813,156 + -0.2 813,156 + +2.1 813,156 + -1.6 813,156 + -0.9 813,157 - -0.5 813,157 - -0.6 813,157 - -0.2 813,157 - -0.2 813,275 + +0.7 813,275 + -0.2 813,276 - +1.5 813,276 - +0.7 813,294 + -0.8 813,294 + -0.9 813,294 + -1.4 813,294 + -1.9 813,295 - -0.3 813,295 - +0.6 813,295 - +0.9 813,295 - +0.3 813,295 - +0.4 813,295 - -0.3 813,295 - -1.2 813,295 - -0.9 813,295 - -0.2 813,295 - +0.5 813,311 - -0.4 813,311 - +0.0 813,445 - EX31_RS20820 0.17 +1.2 813,511 + EX31_RS20820 0.25 +1.0 813,511 + EX31_RS20820 0.25 -0.9 813,511 + EX31_RS20820 0.25 +2.1 813,512 - EX31_RS20820 0.25 +0.4 813,512 - EX31_RS20820 0.25 -0.8 813,512 - EX31_RS20820 0.25 +1.1 813,512 - EX31_RS20820 0.25 -0.7 813,536 + EX31_RS20820 0.28 -0.1 813,543 + EX31_RS20820 0.29 -1.8 813,543 + EX31_RS20820 0.29 -1.3 813,543 + EX31_RS20820 0.29 +0.2 813,554 + EX31_RS20820 0.30 -0.1 813,554 + EX31_RS20820 0.30 +0.1 813,554 + EX31_RS20820 0.30 +0.9 813,555 - EX31_RS20820 0.30 +0.4 813,555 - EX31_RS20820 0.30 +0.0 813,597 + EX31_RS20820 0.35 -0.4 813,597 + EX31_RS20820 0.35 -1.0 813,597 + EX31_RS20820 0.35 +0.0 813,597 + EX31_RS20820 0.35 -1.0 813,597 + EX31_RS20820 0.35 +0.6 813,609 + EX31_RS20820 0.36 -0.6 813,609 + EX31_RS20820 0.36 -0.1 813,610 - EX31_RS20820 0.36 -0.9 813,610 - EX31_RS20820 0.36 +0.4 813,627 + EX31_RS20820 0.38 -0.5 813,866 + EX31_RS20820 0.66 +0.1 813,867 - EX31_RS20820 0.67 -0.3 813,867 - EX31_RS20820 0.67 -0.3 813,915 + EX31_RS20820 0.72 -0.3 813,916 - EX31_RS20820 0.72 +0.3 813,916 - EX31_RS20820 0.72 +0.5 814,001 + EX31_RS20820 0.82 +0.9 814,001 + EX31_RS20820 0.82 -0.6 814,002 - EX31_RS20820 0.82 +0.4 814,002 - EX31_RS20820 0.82 +1.0 814,002 - EX31_RS20820 0.82 +0.5 814,049 + EX31_RS20820 0.88 +0.6 814,049 + EX31_RS20820 0.88 -0.0 814,049 + EX31_RS20820 0.88 -1.1 814,049 + EX31_RS20820 0.88 -1.0 814,049 + EX31_RS20820 0.88 +0.1 814,049 + EX31_RS20820 0.88 -0.6 814,049 + EX31_RS20820 0.88 -0.4 814,049 + EX31_RS20820 0.88 +0.1 814,049 + EX31_RS20820 0.88 +0.4 814,049 + EX31_RS20820 0.88 -0.3 814,049 + EX31_RS20820 0.88 -0.6 814,049 + EX31_RS20820 0.88 -0.0 814,050 - EX31_RS20820 0.88 +0.8 814,050 - EX31_RS20820 0.88 -0.1 814,050 - EX31_RS20820 0.88 +0.9 814,050 - EX31_RS20820 0.88 -0.9 814,050 - EX31_RS20820 0.88 +0.7 814,050 - EX31_RS20820 0.88 +2.3 814,050 - EX31_RS20820 0.88 -0.1 814,149 + +1.1 814,149 + -2.5 814,150 - -0.6 814,168 + +0.5 814,168 + -0.1 814,169 - -1.3 814,190 + -0.2 814,273 + -0.5 814,346 + EX31_RS20825 0.17 -1.2 814,346 + EX31_RS20825 0.17 -1.0 814,346 + EX31_RS20825 0.17 -0.5 814,346 + EX31_RS20825 0.17 +0.0 814,346 + EX31_RS20825 0.17 +0.2 814,346 + EX31_RS20825 0.17 -0.7 814,347 - EX31_RS20825 0.17 +2.1 814,347 - EX31_RS20825 0.17 -2.0 814,347 - EX31_RS20825 0.17 +1.6 814,386 + EX31_RS20825 0.21 -1.2 814,433 - EX31_RS20825 0.26 +0.3 814,509 + EX31_RS20825 0.33 -1.9 814,509 + EX31_RS20825 0.33 -0.2 814,509 + EX31_RS20825 0.33 +0.3 814,510 - EX31_RS20825 0.34 -0.8 814,510 - EX31_RS20825 0.34 -0.6 814,520 - EX31_RS20825 0.35 -0.6 814,531 + EX31_RS20825 0.36 +0.2 814,531 + EX31_RS20825 0.36 -0.5 814,532 - EX31_RS20825 0.36 -0.9 814,532 - EX31_RS20825 0.36 -0.5 814,532 - EX31_RS20825 0.36 +0.8 814,544 + EX31_RS20825 0.37 +0.3 814,608 + EX31_RS20825 0.43 +1.7 814,609 - EX31_RS20825 0.44 +1.6 814,624 + EX31_RS20825 0.45 +0.3 814,624 + EX31_RS20825 0.45 -0.4 814,681 - EX31_RS20825 0.51 -0.5 814,681 - EX31_RS20825 0.51 -1.2 814,681 - EX31_RS20825 0.51 +0.4 814,681 - EX31_RS20825 0.51 -0.8 814,799 - EX31_RS20825 0.63 -0.3 814,853 + EX31_RS20825 0.68 +1.1 815,132 + +0.5 815,132 + -0.2 815,133 - -0.4
Or see this region's nucleotide sequence