Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20420 and EX31_RS20425 are separated by 139 nucleotides EX31_RS20425 and EX31_RS20430 are separated by 51 nucleotides EX31_RS20430 and EX31_RS20435 are separated by 85 nucleotides EX31_RS20435 and EX31_RS20440 are separated by 118 nucleotides
EX31_RS20420: EX31_RS20420 - GNAT family N-acetyltransferase, at 717,213 to 717,647
_RS20420
EX31_RS20425: EX31_RS20425 - DUF3313 domain-containing protein, at 717,787 to 718,446
_RS20425
EX31_RS20430: EX31_RS20430 - LysR family transcriptional regulator, at 718,498 to 719,391
_RS20430
EX31_RS20435: EX31_RS20435 - NAD(P)H-dependent oxidoreductase, at 719,477 to 720,250
_RS20435
EX31_RS20440: EX31_RS20440 - diguanylate cyclase, at 720,369 to 721,703
_RS20440
Position (kb) 718
719
720 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 717.538 kb on + strand, within EX31_RS20420 at 717.539 kb on - strand, within EX31_RS20420 at 717.565 kb on - strand, within EX31_RS20420 at 717.618 kb on + strand at 717.618 kb on + strand at 717.618 kb on + strand at 717.621 kb on + strand at 717.621 kb on + strand at 717.622 kb on - strand at 717.622 kb on - strand at 717.622 kb on - strand at 717.664 kb on + strand at 717.679 kb on + strand at 717.679 kb on + strand at 717.746 kb on + strand at 717.746 kb on + strand at 717.746 kb on + strand at 717.746 kb on + strand at 717.747 kb on - strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.748 kb on + strand at 717.749 kb on - strand at 717.749 kb on - strand at 717.749 kb on - strand at 717.749 kb on - strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.752 kb on + strand at 717.753 kb on - strand at 717.753 kb on - strand at 717.753 kb on - strand at 717.753 kb on - strand at 717.753 kb on - strand at 717.753 kb on - strand at 717.753 kb on - strand at 717.753 kb on - strand at 717.767 kb on - strand at 717.797 kb on + strand at 717.798 kb on - strand at 717.890 kb on + strand, within EX31_RS20425 at 717.898 kb on + strand, within EX31_RS20425 at 717.985 kb on + strand, within EX31_RS20425 at 717.985 kb on + strand, within EX31_RS20425 at 717.986 kb on - strand, within EX31_RS20425 at 718.201 kb on + strand, within EX31_RS20425 at 718.201 kb on + strand, within EX31_RS20425 at 718.202 kb on - strand, within EX31_RS20425 at 718.202 kb on - strand, within EX31_RS20425 at 718.202 kb on - strand, within EX31_RS20425 at 718.227 kb on + strand, within EX31_RS20425 at 718.269 kb on + strand, within EX31_RS20425 at 718.279 kb on + strand, within EX31_RS20425 at 718.280 kb on - strand, within EX31_RS20425 at 718.280 kb on - strand, within EX31_RS20425 at 718.339 kb on - strand, within EX31_RS20425 at 718.368 kb on + strand, within EX31_RS20425 at 718.406 kb on + strand at 718.406 kb on + strand at 718.407 kb on - strand at 718.407 kb on - strand at 718.407 kb on - strand at 718.407 kb on - strand at 718.410 kb on + strand at 718.410 kb on + strand at 718.484 kb on - strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.574 kb on + strand at 718.575 kb on - strand at 718.575 kb on - strand at 718.575 kb on - strand at 718.575 kb on - strand at 718.575 kb on - strand at 718.575 kb on - strand at 718.575 kb on - strand at 718.575 kb on - strand at 718.575 kb on - strand at 718.575 kb on - strand at 718.575 kb on - strand at 718.575 kb on - strand at 718.575 kb on - strand at 718.575 kb on - strand at 718.575 kb on - strand at 718.608 kb on + strand, within EX31_RS20430 at 718.608 kb on + strand, within EX31_RS20430 at 718.608 kb on + strand, within EX31_RS20430 at 718.608 kb on + strand, within EX31_RS20430 at 718.608 kb on + strand, within EX31_RS20430 at 718.608 kb on + strand, within EX31_RS20430 at 718.608 kb on + strand, within EX31_RS20430 at 718.608 kb on + strand, within EX31_RS20430 at 718.609 kb on - strand, within EX31_RS20430 at 718.609 kb on - strand, within EX31_RS20430 at 718.609 kb on - strand, within EX31_RS20430 at 718.609 kb on - strand, within EX31_RS20430 at 718.612 kb on - strand, within EX31_RS20430 at 718.612 kb on - strand, within EX31_RS20430 at 718.761 kb on + strand, within EX31_RS20430 at 718.769 kb on + strand, within EX31_RS20430 at 718.770 kb on - strand, within EX31_RS20430 at 718.832 kb on - strand, within EX31_RS20430 at 718.877 kb on + strand, within EX31_RS20430 at 718.877 kb on + strand, within EX31_RS20430 at 718.877 kb on + strand, within EX31_RS20430 at 718.877 kb on + strand, within EX31_RS20430 at 718.878 kb on - strand, within EX31_RS20430 at 718.895 kb on - strand, within EX31_RS20430 at 718.954 kb on - strand, within EX31_RS20430 at 719.028 kb on + strand, within EX31_RS20430 at 719.028 kb on + strand, within EX31_RS20430 at 719.028 kb on + strand, within EX31_RS20430 at 719.032 kb on + strand, within EX31_RS20430 at 719.032 kb on + strand, within EX31_RS20430 at 719.032 kb on + strand, within EX31_RS20430 at 719.032 kb on + strand, within EX31_RS20430 at 719.032 kb on + strand, within EX31_RS20430 at 719.033 kb on - strand, within EX31_RS20430 at 719.033 kb on - strand, within EX31_RS20430 at 719.097 kb on + strand, within EX31_RS20430 at 719.098 kb on - strand, within EX31_RS20430 at 719.138 kb on - strand, within EX31_RS20430 at 719.138 kb on - strand, within EX31_RS20430 at 719.182 kb on + strand, within EX31_RS20430 at 719.182 kb on + strand, within EX31_RS20430 at 719.182 kb on + strand, within EX31_RS20430 at 719.182 kb on + strand, within EX31_RS20430 at 719.183 kb on - strand, within EX31_RS20430 at 719.183 kb on - strand, within EX31_RS20430 at 719.396 kb on - strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.405 kb on - strand at 719.406 kb on + strand at 719.426 kb on + strand at 719.426 kb on + strand at 719.427 kb on - strand at 719.428 kb on + strand at 719.428 kb on + strand at 719.443 kb on + strand at 719.443 kb on + strand at 719.443 kb on + strand at 719.444 kb on - strand at 719.444 kb on - strand at 719.444 kb on - strand at 719.444 kb on - strand at 719.444 kb on - strand at 719.454 kb on + strand at 719.462 kb on - strand at 719.484 kb on + strand at 719.485 kb on - strand at 719.485 kb on - strand at 719.485 kb on - strand at 719.495 kb on + strand at 719.495 kb on + strand at 719.496 kb on - strand at 719.595 kb on - strand, within EX31_RS20435 at 719.595 kb on - strand, within EX31_RS20435 at 719.633 kb on - strand, within EX31_RS20435 at 719.687 kb on + strand, within EX31_RS20435 at 719.736 kb on + strand, within EX31_RS20435 at 719.736 kb on + strand, within EX31_RS20435 at 719.736 kb on + strand, within EX31_RS20435 at 719.757 kb on + strand, within EX31_RS20435 at 719.757 kb on + strand, within EX31_RS20435 at 719.758 kb on - strand, within EX31_RS20435 at 719.758 kb on - strand, within EX31_RS20435 at 719.773 kb on - strand, within EX31_RS20435 at 719.823 kb on - strand, within EX31_RS20435 at 719.951 kb on + strand, within EX31_RS20435 at 719.951 kb on + strand, within EX31_RS20435 at 719.952 kb on - strand, within EX31_RS20435 at 719.952 kb on - strand, within EX31_RS20435 at 719.952 kb on - strand, within EX31_RS20435 at 719.952 kb on - strand, within EX31_RS20435 at 719.968 kb on + strand, within EX31_RS20435 at 720.124 kb on + strand, within EX31_RS20435 at 720.125 kb on - strand, within EX31_RS20435 at 720.129 kb on + strand, within EX31_RS20435 at 720.176 kb on + strand at 720.177 kb on - strand at 720.248 kb on + strand at 720.291 kb on - strand at 720.295 kb on + strand at 720.296 kb on - strand at 720.322 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 717,538 + EX31_RS20420 0.75 -1.0 717,539 - EX31_RS20420 0.75 +1.0 717,565 - EX31_RS20420 0.81 -1.3 717,618 + +1.0 717,618 + +0.4 717,618 + -0.3 717,621 + +0.4 717,621 + +0.0 717,622 - -0.6 717,622 - -0.0 717,622 - -1.2 717,664 + +0.0 717,679 + +0.3 717,679 + +0.2 717,746 + -2.7 717,746 + +0.5 717,746 + +1.3 717,746 + -0.4 717,747 - -0.1 717,748 + +0.1 717,748 + +2.2 717,748 + +0.9 717,748 + -0.6 717,748 + -1.5 717,748 + -1.6 717,748 + +0.0 717,748 + -0.6 717,748 + +1.1 717,748 + +0.3 717,748 + -0.3 717,748 + -0.9 717,749 - -0.7 717,749 - -2.3 717,749 - -0.2 717,749 - -0.6 717,752 + -0.6 717,752 + -0.2 717,752 + -0.9 717,752 + -0.8 717,752 + +0.3 717,752 + -0.0 717,752 + +1.1 717,752 + +0.4 717,752 + -0.3 717,752 + -0.5 717,752 + -0.4 717,752 + -0.7 717,752 + +0.5 717,752 + -0.2 717,752 + +0.8 717,752 + +1.2 717,752 + +0.2 717,752 + +1.3 717,752 + -0.4 717,752 + +0.7 717,752 + +0.9 717,752 + -1.3 717,752 + -0.4 717,752 + -2.5 717,752 + -0.2 717,753 - +2.1 717,753 - +0.8 717,753 - +1.7 717,753 - +0.7 717,753 - -0.3 717,753 - -0.5 717,753 - +0.7 717,753 - +0.1 717,767 - +0.5 717,797 + -1.5 717,798 - +0.2 717,890 + EX31_RS20425 0.16 -1.3 717,898 + EX31_RS20425 0.17 -0.3 717,985 + EX31_RS20425 0.30 -0.6 717,985 + EX31_RS20425 0.30 +0.1 717,986 - EX31_RS20425 0.30 +0.0 718,201 + EX31_RS20425 0.63 -0.7 718,201 + EX31_RS20425 0.63 +1.6 718,202 - EX31_RS20425 0.63 +0.4 718,202 - EX31_RS20425 0.63 -0.4 718,202 - EX31_RS20425 0.63 +0.2 718,227 + EX31_RS20425 0.67 -0.9 718,269 + EX31_RS20425 0.73 -0.3 718,279 + EX31_RS20425 0.75 +0.3 718,280 - EX31_RS20425 0.75 -0.5 718,280 - EX31_RS20425 0.75 +0.8 718,339 - EX31_RS20425 0.84 +0.4 718,368 + EX31_RS20425 0.88 -0.8 718,406 + +0.7 718,406 + -0.3 718,407 - +1.4 718,407 - +0.5 718,407 - -0.3 718,407 - +1.1 718,410 + -0.4 718,410 + +1.4 718,484 - +0.8 718,574 + -1.0 718,574 + +0.6 718,574 + -0.3 718,574 + +0.1 718,574 + +1.4 718,574 + +0.4 718,574 + -0.1 718,574 + -1.0 718,574 + -0.6 718,574 + -2.0 718,574 + +0.9 718,574 + +0.1 718,574 + +0.5 718,574 + +0.3 718,574 + +0.7 718,574 + -0.9 718,574 + +0.7 718,574 + +1.0 718,574 + -0.6 718,574 + +0.8 718,574 + -0.3 718,574 + -1.0 718,575 - +0.1 718,575 - -0.6 718,575 - +0.5 718,575 - -0.3 718,575 - +0.8 718,575 - +0.4 718,575 - +0.3 718,575 - +0.1 718,575 - -1.0 718,575 - +0.6 718,575 - +0.5 718,575 - +0.7 718,575 - +0.1 718,575 - +1.1 718,575 - -0.4 718,608 + EX31_RS20430 0.12 +0.3 718,608 + EX31_RS20430 0.12 +0.8 718,608 + EX31_RS20430 0.12 -2.3 718,608 + EX31_RS20430 0.12 -1.9 718,608 + EX31_RS20430 0.12 +1.0 718,608 + EX31_RS20430 0.12 -0.5 718,608 + EX31_RS20430 0.12 -0.1 718,608 + EX31_RS20430 0.12 +0.4 718,609 - EX31_RS20430 0.12 +0.3 718,609 - EX31_RS20430 0.12 +0.1 718,609 - EX31_RS20430 0.12 -1.6 718,609 - EX31_RS20430 0.12 -1.5 718,612 - EX31_RS20430 0.13 -0.5 718,612 - EX31_RS20430 0.13 -0.2 718,761 + EX31_RS20430 0.29 -2.5 718,769 + EX31_RS20430 0.30 -1.0 718,770 - EX31_RS20430 0.30 -0.5 718,832 - EX31_RS20430 0.37 +0.8 718,877 + EX31_RS20430 0.42 -1.1 718,877 + EX31_RS20430 0.42 -1.0 718,877 + EX31_RS20430 0.42 -2.0 718,877 + EX31_RS20430 0.42 +0.4 718,878 - EX31_RS20430 0.43 +0.4 718,895 - EX31_RS20430 0.44 +0.5 718,954 - EX31_RS20430 0.51 +1.1 719,028 + EX31_RS20430 0.59 -0.2 719,028 + EX31_RS20430 0.59 +0.1 719,028 + EX31_RS20430 0.59 -0.5 719,032 + EX31_RS20430 0.60 +0.5 719,032 + EX31_RS20430 0.60 -0.4 719,032 + EX31_RS20430 0.60 -1.9 719,032 + EX31_RS20430 0.60 -0.2 719,032 + EX31_RS20430 0.60 +1.0 719,033 - EX31_RS20430 0.60 -2.8 719,033 - EX31_RS20430 0.60 -0.8 719,097 + EX31_RS20430 0.67 +0.9 719,098 - EX31_RS20430 0.67 -0.9 719,138 - EX31_RS20430 0.72 +0.8 719,138 - EX31_RS20430 0.72 -0.3 719,182 + EX31_RS20430 0.77 +1.3 719,182 + EX31_RS20430 0.77 -0.8 719,182 + EX31_RS20430 0.77 +0.1 719,182 + EX31_RS20430 0.77 +0.6 719,183 - EX31_RS20430 0.77 -0.0 719,183 - EX31_RS20430 0.77 +0.8 719,396 - +0.3 719,404 + -0.2 719,404 + +0.6 719,404 + -0.4 719,404 + -2.7 719,404 + -0.3 719,404 + -0.5 719,405 - -0.6 719,406 + -0.3 719,426 + -0.1 719,426 + -0.0 719,427 - +1.1 719,428 + +0.1 719,428 + +0.5 719,443 + -0.1 719,443 + -1.8 719,443 + +1.0 719,444 - +1.2 719,444 - -0.2 719,444 - +0.2 719,444 - -0.6 719,444 - -1.4 719,454 + -0.3 719,462 - -0.8 719,484 + +0.7 719,485 - -1.4 719,485 - -0.3 719,485 - +1.5 719,495 + -0.8 719,495 + +2.1 719,496 - -0.3 719,595 - EX31_RS20435 0.15 -0.2 719,595 - EX31_RS20435 0.15 +1.1 719,633 - EX31_RS20435 0.20 -0.4 719,687 + EX31_RS20435 0.27 -0.6 719,736 + EX31_RS20435 0.33 +0.8 719,736 + EX31_RS20435 0.33 -0.5 719,736 + EX31_RS20435 0.33 -0.1 719,757 + EX31_RS20435 0.36 +1.5 719,757 + EX31_RS20435 0.36 -0.2 719,758 - EX31_RS20435 0.36 -0.7 719,758 - EX31_RS20435 0.36 -0.8 719,773 - EX31_RS20435 0.38 +0.8 719,823 - EX31_RS20435 0.45 -0.2 719,951 + EX31_RS20435 0.61 -0.9 719,951 + EX31_RS20435 0.61 -2.6 719,952 - EX31_RS20435 0.61 +0.5 719,952 - EX31_RS20435 0.61 +1.5 719,952 - EX31_RS20435 0.61 +0.4 719,952 - EX31_RS20435 0.61 +0.6 719,968 + EX31_RS20435 0.63 +0.3 720,124 + EX31_RS20435 0.84 +0.2 720,125 - EX31_RS20435 0.84 -2.3 720,129 + EX31_RS20435 0.84 +0.8 720,176 + -0.5 720,177 - +1.0 720,248 + +0.1 720,291 - -0.5 720,295 + +0.1 720,296 - +0.5 720,322 - -0.1
Or see this region's nucleotide sequence