Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20400 and EX31_RS20405 are separated by 36 nucleotides EX31_RS20405 and EX31_RS20410 overlap by 16 nucleotides EX31_RS20410 and EX31_RS20415 overlap by 17 nucleotides
EX31_RS20400: EX31_RS20400 - glycosyltransferase family 2 protein, at 712,808 to 713,797
_RS20400
EX31_RS20405: EX31_RS20405 - class I SAM-dependent methyltransferase, at 713,834 to 714,466
_RS20405
EX31_RS20410: EX31_RS20410 - hypothetical protein, at 714,451 to 715,359
_RS20410
EX31_RS20415: EX31_RS20415 - phospholipid carrier-dependent glycosyltransferase, at 715,343 to 717,115
_RS20415
Position (kb) 714
715
716 Strain fitness (log2 ratio) -2
-1
0
1
2 at 713.559 kb on + strand, within EX31_RS20400 at 713.560 kb on - strand, within EX31_RS20400 at 713.568 kb on + strand, within EX31_RS20400 at 713.569 kb on - strand, within EX31_RS20400 at 713.604 kb on + strand, within EX31_RS20400 at 713.605 kb on - strand, within EX31_RS20400 at 713.605 kb on - strand, within EX31_RS20400 at 713.626 kb on + strand, within EX31_RS20400 at 713.626 kb on + strand, within EX31_RS20400 at 713.626 kb on + strand, within EX31_RS20400 at 713.700 kb on + strand at 713.701 kb on - strand at 713.712 kb on + strand at 713.817 kb on + strand at 713.852 kb on - strand at 713.852 kb on - strand at 713.894 kb on + strand at 714.023 kb on - strand, within EX31_RS20405 at 714.023 kb on - strand, within EX31_RS20405 at 714.082 kb on + strand, within EX31_RS20405 at 714.261 kb on - strand, within EX31_RS20405 at 714.267 kb on + strand, within EX31_RS20405 at 714.271 kb on - strand, within EX31_RS20405 at 714.300 kb on + strand, within EX31_RS20405 at 714.304 kb on + strand, within EX31_RS20405 at 714.305 kb on - strand, within EX31_RS20405 at 714.323 kb on + strand, within EX31_RS20405 at 714.323 kb on + strand, within EX31_RS20405 at 714.324 kb on - strand, within EX31_RS20405 at 714.358 kb on - strand, within EX31_RS20405 at 714.629 kb on + strand, within EX31_RS20410 at 714.629 kb on + strand, within EX31_RS20410 at 714.630 kb on - strand, within EX31_RS20410 at 714.660 kb on + strand, within EX31_RS20410 at 714.751 kb on + strand, within EX31_RS20410 at 714.792 kb on + strand, within EX31_RS20410 at 714.918 kb on - strand, within EX31_RS20410 at 714.951 kb on - strand, within EX31_RS20410 at 715.111 kb on - strand, within EX31_RS20410 at 715.129 kb on + strand, within EX31_RS20410 at 715.188 kb on - strand, within EX31_RS20410 at 715.309 kb on + strand at 715.506 kb on + strand at 715.566 kb on - strand, within EX31_RS20415 at 715.744 kb on + strand, within EX31_RS20415 at 715.746 kb on + strand, within EX31_RS20415 at 715.751 kb on - strand, within EX31_RS20415 at 715.751 kb on - strand, within EX31_RS20415 at 715.835 kb on + strand, within EX31_RS20415 at 716.019 kb on + strand, within EX31_RS20415 at 716.162 kb on + strand, within EX31_RS20415 at 716.167 kb on + strand, within EX31_RS20415 at 716.167 kb on + strand, within EX31_RS20415 at 716.349 kb on + strand, within EX31_RS20415
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 713,559 + EX31_RS20400 0.76 +0.2 713,560 - EX31_RS20400 0.76 +1.8 713,568 + EX31_RS20400 0.77 +0.6 713,569 - EX31_RS20400 0.77 +1.5 713,604 + EX31_RS20400 0.80 -0.6 713,605 - EX31_RS20400 0.81 -0.3 713,605 - EX31_RS20400 0.81 +1.2 713,626 + EX31_RS20400 0.83 -1.1 713,626 + EX31_RS20400 0.83 -0.9 713,626 + EX31_RS20400 0.83 -0.3 713,700 + +1.9 713,701 - +0.8 713,712 + -0.5 713,817 + +0.6 713,852 - -0.9 713,852 - +0.1 713,894 + -1.0 714,023 - EX31_RS20405 0.30 +1.4 714,023 - EX31_RS20405 0.30 -0.9 714,082 + EX31_RS20405 0.39 +1.2 714,261 - EX31_RS20405 0.67 +1.3 714,267 + EX31_RS20405 0.68 -0.8 714,271 - EX31_RS20405 0.69 +1.9 714,300 + EX31_RS20405 0.74 +0.3 714,304 + EX31_RS20405 0.74 -0.7 714,305 - EX31_RS20405 0.74 -0.3 714,323 + EX31_RS20405 0.77 +1.2 714,323 + EX31_RS20405 0.77 -0.8 714,324 - EX31_RS20405 0.77 +0.3 714,358 - EX31_RS20405 0.83 +0.7 714,629 + EX31_RS20410 0.20 +2.1 714,629 + EX31_RS20410 0.20 +1.1 714,630 - EX31_RS20410 0.20 -0.6 714,660 + EX31_RS20410 0.23 +0.5 714,751 + EX31_RS20410 0.33 -0.8 714,792 + EX31_RS20410 0.38 +1.1 714,918 - EX31_RS20410 0.51 +0.2 714,951 - EX31_RS20410 0.55 +0.8 715,111 - EX31_RS20410 0.73 +0.8 715,129 + EX31_RS20410 0.75 -0.2 715,188 - EX31_RS20410 0.81 -1.0 715,309 + -0.7 715,506 + +0.2 715,566 - EX31_RS20415 0.13 -1.9 715,744 + EX31_RS20415 0.23 +1.1 715,746 + EX31_RS20415 0.23 -1.0 715,751 - EX31_RS20415 0.23 -0.7 715,751 - EX31_RS20415 0.23 +0.6 715,835 + EX31_RS20415 0.28 -1.1 716,019 + EX31_RS20415 0.38 +0.1 716,162 + EX31_RS20415 0.46 +0.5 716,167 + EX31_RS20415 0.46 +1.1 716,167 + EX31_RS20415 0.46 -1.2 716,349 + EX31_RS20415 0.57 +0.1
Or see this region's nucleotide sequence