Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20385 and EX31_RS20390 are separated by 7 nucleotides EX31_RS20390 and EX31_RS20395 overlap by 4 nucleotides EX31_RS20395 and EX31_RS20400 are separated by 423 nucleotides
EX31_RS20385: EX31_RS20385 - hypothetical protein, at 709,922 to 710,770
_RS20385
EX31_RS20390: EX31_RS20390 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 710,778 to 711,578
_RS20390
EX31_RS20395: EX31_RS20395 - hydroxyethylthiazole kinase, at 711,575 to 712,384
_RS20395
EX31_RS20400: EX31_RS20400 - glycosyltransferase family 2 protein, at 712,808 to 713,797
_RS20400
Position (kb) 711
712
713 Strain fitness (log2 ratio) -2
-1
0
1
2 at 710.603 kb on + strand, within EX31_RS20385 at 710.604 kb on - strand, within EX31_RS20385 at 710.604 kb on - strand, within EX31_RS20385 at 710.629 kb on + strand, within EX31_RS20385 at 710.768 kb on + strand at 710.768 kb on + strand at 710.768 kb on + strand at 710.859 kb on + strand, within EX31_RS20390 at 710.859 kb on + strand, within EX31_RS20390 at 710.859 kb on + strand, within EX31_RS20390 at 710.860 kb on - strand, within EX31_RS20390 at 710.860 kb on - strand, within EX31_RS20390 at 710.860 kb on - strand, within EX31_RS20390 at 710.864 kb on - strand, within EX31_RS20390 at 710.881 kb on - strand, within EX31_RS20390 at 710.913 kb on + strand, within EX31_RS20390 at 710.914 kb on - strand, within EX31_RS20390 at 710.914 kb on - strand, within EX31_RS20390 at 710.957 kb on + strand, within EX31_RS20390 at 710.957 kb on + strand, within EX31_RS20390 at 710.958 kb on - strand, within EX31_RS20390 at 710.958 kb on - strand, within EX31_RS20390 at 711.173 kb on + strand, within EX31_RS20390 at 711.232 kb on - strand, within EX31_RS20390 at 711.289 kb on + strand, within EX31_RS20390 at 711.290 kb on - strand, within EX31_RS20390 at 711.419 kb on + strand, within EX31_RS20390 at 711.420 kb on - strand, within EX31_RS20390 at 711.421 kb on + strand, within EX31_RS20390 at 711.422 kb on - strand, within EX31_RS20390 at 711.422 kb on - strand, within EX31_RS20390 at 711.422 kb on - strand, within EX31_RS20390 at 711.422 kb on - strand, within EX31_RS20390 at 711.566 kb on + strand at 711.567 kb on - strand at 711.569 kb on + strand at 711.569 kb on + strand at 711.569 kb on + strand at 711.569 kb on + strand at 711.570 kb on - strand at 711.570 kb on - strand at 711.614 kb on + strand at 711.614 kb on + strand at 711.615 kb on - strand at 711.615 kb on - strand at 711.615 kb on - strand at 711.615 kb on - strand at 711.619 kb on - strand at 711.619 kb on - strand at 711.625 kb on + strand at 711.633 kb on + strand at 711.771 kb on - strand, within EX31_RS20395 at 711.852 kb on + strand, within EX31_RS20395 at 711.853 kb on - strand, within EX31_RS20395 at 712.021 kb on + strand, within EX31_RS20395 at 712.022 kb on - strand, within EX31_RS20395 at 712.022 kb on - strand, within EX31_RS20395 at 712.022 kb on - strand, within EX31_RS20395 at 712.178 kb on + strand, within EX31_RS20395 at 712.369 kb on + strand at 712.370 kb on - strand at 712.384 kb on + strand at 712.384 kb on + strand at 712.385 kb on - strand at 712.385 kb on - strand at 712.385 kb on - strand at 712.385 kb on - strand at 712.385 kb on - strand at 712.385 kb on - strand at 712.385 kb on - strand at 712.385 kb on - strand at 712.427 kb on + strand at 712.428 kb on - strand at 712.464 kb on + strand at 712.464 kb on + strand at 712.465 kb on - strand at 712.465 kb on - strand at 712.465 kb on - strand at 712.522 kb on + strand at 712.579 kb on + strand at 712.580 kb on - strand at 712.582 kb on + strand at 712.593 kb on + strand at 712.598 kb on + strand at 712.598 kb on + strand at 712.599 kb on - strand at 712.599 kb on - strand at 712.625 kb on + strand at 712.628 kb on + strand at 712.648 kb on - strand at 712.648 kb on - strand at 712.674 kb on + strand at 712.674 kb on + strand at 712.674 kb on + strand at 712.675 kb on - strand at 712.676 kb on + strand at 712.677 kb on - strand at 712.677 kb on - strand at 712.688 kb on + strand at 712.688 kb on + strand at 712.688 kb on + strand at 712.689 kb on - strand at 712.689 kb on - strand at 712.714 kb on + strand at 712.730 kb on + strand at 712.761 kb on - strand at 712.780 kb on + strand at 712.781 kb on - strand at 712.821 kb on + strand at 712.900 kb on + strand at 712.942 kb on + strand, within EX31_RS20400 at 712.942 kb on + strand, within EX31_RS20400 at 712.942 kb on + strand, within EX31_RS20400 at 712.942 kb on + strand, within EX31_RS20400 at 712.943 kb on - strand, within EX31_RS20400 at 712.943 kb on - strand, within EX31_RS20400 at 712.980 kb on + strand, within EX31_RS20400 at 712.980 kb on + strand, within EX31_RS20400 at 712.981 kb on - strand, within EX31_RS20400 at 712.990 kb on + strand, within EX31_RS20400 at 712.990 kb on + strand, within EX31_RS20400 at 712.990 kb on + strand, within EX31_RS20400 at 712.991 kb on - strand, within EX31_RS20400 at 712.991 kb on - strand, within EX31_RS20400 at 713.012 kb on + strand, within EX31_RS20400 at 713.012 kb on + strand, within EX31_RS20400 at 713.013 kb on - strand, within EX31_RS20400 at 713.071 kb on + strand, within EX31_RS20400 at 713.092 kb on + strand, within EX31_RS20400 at 713.092 kb on + strand, within EX31_RS20400 at 713.093 kb on - strand, within EX31_RS20400 at 713.093 kb on - strand, within EX31_RS20400 at 713.104 kb on + strand, within EX31_RS20400 at 713.104 kb on + strand, within EX31_RS20400 at 713.128 kb on - strand, within EX31_RS20400 at 713.128 kb on - strand, within EX31_RS20400 at 713.311 kb on + strand, within EX31_RS20400 at 713.328 kb on - strand, within EX31_RS20400 at 713.329 kb on + strand, within EX31_RS20400 at 713.329 kb on + strand, within EX31_RS20400 at 713.329 kb on + strand, within EX31_RS20400 at 713.330 kb on - strand, within EX31_RS20400 at 713.333 kb on + strand, within EX31_RS20400 at 713.333 kb on + strand, within EX31_RS20400 at 713.333 kb on + strand, within EX31_RS20400 at 713.334 kb on - strand, within EX31_RS20400 at 713.335 kb on + strand, within EX31_RS20400 at 713.335 kb on + strand, within EX31_RS20400 at 713.347 kb on + strand, within EX31_RS20400 at 713.377 kb on + strand, within EX31_RS20400
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 710,603 + EX31_RS20385 0.80 -0.9 710,604 - EX31_RS20385 0.80 -0.7 710,604 - EX31_RS20385 0.80 -0.1 710,629 + EX31_RS20385 0.83 -0.6 710,768 + +0.6 710,768 + -0.4 710,768 + -0.4 710,859 + EX31_RS20390 0.10 -0.7 710,859 + EX31_RS20390 0.10 +0.9 710,859 + EX31_RS20390 0.10 +1.3 710,860 - EX31_RS20390 0.10 +0.0 710,860 - EX31_RS20390 0.10 +0.9 710,860 - EX31_RS20390 0.10 -0.3 710,864 - EX31_RS20390 0.11 -0.5 710,881 - EX31_RS20390 0.13 -0.5 710,913 + EX31_RS20390 0.17 +0.9 710,914 - EX31_RS20390 0.17 -1.0 710,914 - EX31_RS20390 0.17 -0.5 710,957 + EX31_RS20390 0.22 +0.4 710,957 + EX31_RS20390 0.22 -0.1 710,958 - EX31_RS20390 0.22 -0.1 710,958 - EX31_RS20390 0.22 +0.5 711,173 + EX31_RS20390 0.49 +1.5 711,232 - EX31_RS20390 0.57 +0.2 711,289 + EX31_RS20390 0.64 +0.3 711,290 - EX31_RS20390 0.64 +0.2 711,419 + EX31_RS20390 0.80 -0.6 711,420 - EX31_RS20390 0.80 +1.1 711,421 + EX31_RS20390 0.80 -1.2 711,422 - EX31_RS20390 0.80 -0.7 711,422 - EX31_RS20390 0.80 -0.3 711,422 - EX31_RS20390 0.80 +0.9 711,422 - EX31_RS20390 0.80 +0.1 711,566 + -0.2 711,567 - -0.0 711,569 + -0.1 711,569 + +0.3 711,569 + +0.3 711,569 + +0.4 711,570 - -1.3 711,570 - -0.3 711,614 + -0.0 711,614 + +0.9 711,615 - -1.0 711,615 - +0.5 711,615 - -0.7 711,615 - +0.0 711,619 - -0.1 711,619 - -1.0 711,625 + -0.9 711,633 + +0.5 711,771 - EX31_RS20395 0.24 +0.2 711,852 + EX31_RS20395 0.34 -0.5 711,853 - EX31_RS20395 0.34 -0.7 712,021 + EX31_RS20395 0.55 +1.1 712,022 - EX31_RS20395 0.55 +1.0 712,022 - EX31_RS20395 0.55 -1.2 712,022 - EX31_RS20395 0.55 -0.1 712,178 + EX31_RS20395 0.74 -1.2 712,369 + +0.3 712,370 - -0.5 712,384 + +0.1 712,384 + -0.3 712,385 - -0.0 712,385 - +0.7 712,385 - -0.0 712,385 - +1.2 712,385 - -0.9 712,385 - +0.3 712,385 - -1.3 712,385 - -0.4 712,427 + +0.7 712,428 - -0.9 712,464 + +0.3 712,464 + -0.3 712,465 - +0.0 712,465 - +0.2 712,465 - +1.5 712,522 + -1.2 712,579 + -0.3 712,580 - +0.5 712,582 + -0.2 712,593 + +0.5 712,598 + +2.1 712,598 + -0.9 712,599 - +0.1 712,599 - +0.9 712,625 + -0.0 712,628 + -0.6 712,648 - -1.2 712,648 - -0.0 712,674 + -0.0 712,674 + +1.0 712,674 + +0.6 712,675 - -0.9 712,676 + -2.2 712,677 - +0.2 712,677 - -1.3 712,688 + -0.4 712,688 + +1.6 712,688 + +0.6 712,689 - +1.4 712,689 - -0.8 712,714 + +0.3 712,730 + +0.4 712,761 - -0.1 712,780 + +1.0 712,781 - +1.1 712,821 + -1.3 712,900 + -0.3 712,942 + EX31_RS20400 0.14 +0.4 712,942 + EX31_RS20400 0.14 -0.2 712,942 + EX31_RS20400 0.14 -0.9 712,942 + EX31_RS20400 0.14 -0.6 712,943 - EX31_RS20400 0.14 +2.1 712,943 - EX31_RS20400 0.14 -0.2 712,980 + EX31_RS20400 0.17 +0.1 712,980 + EX31_RS20400 0.17 +0.8 712,981 - EX31_RS20400 0.17 +0.5 712,990 + EX31_RS20400 0.18 -0.3 712,990 + EX31_RS20400 0.18 +0.2 712,990 + EX31_RS20400 0.18 +0.5 712,991 - EX31_RS20400 0.18 +0.2 712,991 - EX31_RS20400 0.18 +1.2 713,012 + EX31_RS20400 0.21 -0.2 713,012 + EX31_RS20400 0.21 -0.0 713,013 - EX31_RS20400 0.21 +0.6 713,071 + EX31_RS20400 0.27 -0.9 713,092 + EX31_RS20400 0.29 -0.6 713,092 + EX31_RS20400 0.29 +0.6 713,093 - EX31_RS20400 0.29 +0.5 713,093 - EX31_RS20400 0.29 +0.2 713,104 + EX31_RS20400 0.30 +0.3 713,104 + EX31_RS20400 0.30 -0.6 713,128 - EX31_RS20400 0.32 -2.5 713,128 - EX31_RS20400 0.32 +1.3 713,311 + EX31_RS20400 0.51 +1.1 713,328 - EX31_RS20400 0.53 +0.7 713,329 + EX31_RS20400 0.53 +0.2 713,329 + EX31_RS20400 0.53 +0.4 713,329 + EX31_RS20400 0.53 +0.5 713,330 - EX31_RS20400 0.53 -0.3 713,333 + EX31_RS20400 0.53 +0.1 713,333 + EX31_RS20400 0.53 -0.8 713,333 + EX31_RS20400 0.53 +0.1 713,334 - EX31_RS20400 0.53 -0.5 713,335 + EX31_RS20400 0.53 +0.2 713,335 + EX31_RS20400 0.53 +0.5 713,347 + EX31_RS20400 0.54 +1.4 713,377 + EX31_RS20400 0.57 +0.2
Or see this region's nucleotide sequence