Strain Fitness in Rahnella sp. WP5 around EX31_RS19345

Experiment: Ammonium chloride (N); RCH2 media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS19340 and EX31_RS19345 overlap by 1 nucleotidesEX31_RS19345 and EX31_RS19350 overlap by 14 nucleotidesEX31_RS19350 and EX31_RS19355 overlap by 4 nucleotides EX31_RS19335: EX31_RS19335 - o-succinylbenzoate--CoA ligase, at 492,761 to 494,134 _RS19335 EX31_RS19340: EX31_RS19340 - o-succinylbenzoate synthase, at 494,119 to 495,120 _RS19340 EX31_RS19345: EX31_RS19345 - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 495,120 to 495,977 _RS19345 EX31_RS19350: EX31_RS19350 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 495,964 to 496,761 _RS19350 EX31_RS19355: EX31_RS19355 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase, at 496,758 to 498,431 _RS19355 Position (kb) 495 496Strain fitness (log2 ratio) -2 -1 0 1 2at 494.428 kb on - strand, within EX31_RS19340at 494.649 kb on + strand, within EX31_RS19340at 494.650 kb on - strand, within EX31_RS19340at 494.650 kb on - strand, within EX31_RS19340at 494.650 kb on - strand, within EX31_RS19340at 494.650 kb on - strand, within EX31_RS19340at 494.650 kb on - strand, within EX31_RS19340at 494.750 kb on + strand, within EX31_RS19340at 494.821 kb on + strand, within EX31_RS19340at 494.863 kb on + strand, within EX31_RS19340at 494.863 kb on + strand, within EX31_RS19340at 494.967 kb on + strand, within EX31_RS19340at 494.967 kb on + strand, within EX31_RS19340at 494.968 kb on - strand, within EX31_RS19340at 494.968 kb on - strand, within EX31_RS19340at 494.968 kb on - strand, within EX31_RS19340at 495.096 kb on - strandat 495.122 kb on - strandat 495.128 kb on + strandat 495.129 kb on - strandat 495.129 kb on - strandat 495.129 kb on - strandat 495.205 kb on + strandat 495.205 kb on + strandat 495.205 kb on + strandat 495.205 kb on + strandat 495.205 kb on + strandat 495.205 kb on + strandat 495.205 kb on + strandat 495.206 kb on - strand, within EX31_RS19345at 495.206 kb on - strand, within EX31_RS19345at 495.206 kb on - strand, within EX31_RS19345at 495.206 kb on - strand, within EX31_RS19345at 495.366 kb on + strand, within EX31_RS19345at 495.366 kb on + strand, within EX31_RS19345at 495.366 kb on + strand, within EX31_RS19345at 495.367 kb on - strand, within EX31_RS19345at 495.367 kb on - strand, within EX31_RS19345at 495.367 kb on - strand, within EX31_RS19345at 495.410 kb on - strand, within EX31_RS19345at 495.410 kb on - strand, within EX31_RS19345at 495.468 kb on - strand, within EX31_RS19345at 495.468 kb on - strand, within EX31_RS19345at 495.468 kb on - strand, within EX31_RS19345at 495.469 kb on + strand, within EX31_RS19345at 495.469 kb on + strand, within EX31_RS19345at 495.469 kb on + strand, within EX31_RS19345at 495.469 kb on + strand, within EX31_RS19345at 495.470 kb on - strand, within EX31_RS19345at 495.470 kb on - strand, within EX31_RS19345at 495.470 kb on - strand, within EX31_RS19345at 495.470 kb on - strand, within EX31_RS19345at 495.470 kb on - strand, within EX31_RS19345at 495.470 kb on - strand, within EX31_RS19345at 495.470 kb on - strand, within EX31_RS19345at 495.470 kb on - strand, within EX31_RS19345at 495.470 kb on - strand, within EX31_RS19345at 495.470 kb on - strand, within EX31_RS19345at 495.470 kb on - strand, within EX31_RS19345at 495.470 kb on - strand, within EX31_RS19345at 495.470 kb on - strand, within EX31_RS19345at 495.533 kb on + strand, within EX31_RS19345at 495.563 kb on + strand, within EX31_RS19345at 495.563 kb on + strand, within EX31_RS19345at 495.564 kb on - strand, within EX31_RS19345at 495.564 kb on - strand, within EX31_RS19345at 495.564 kb on - strand, within EX31_RS19345at 495.564 kb on - strand, within EX31_RS19345at 495.564 kb on - strand, within EX31_RS19345at 495.872 kb on + strand, within EX31_RS19345at 495.905 kb on + strandat 495.906 kb on - strandat 495.906 kb on - strandat 495.906 kb on - strandat 495.924 kb on - strandat 495.924 kb on - strandat 495.949 kb on + strandat 495.970 kb on + strandat 495.971 kb on - strandat 496.005 kb on + strandat 496.005 kb on + strandat 496.005 kb on + strandat 496.006 kb on - strandat 496.008 kb on + strandat 496.008 kb on + strandat 496.008 kb on + strandat 496.084 kb on + strand, within EX31_RS19350at 496.084 kb on + strand, within EX31_RS19350at 496.117 kb on - strand, within EX31_RS19350at 496.183 kb on + strand, within EX31_RS19350at 496.184 kb on - strand, within EX31_RS19350at 496.342 kb on + strand, within EX31_RS19350at 496.342 kb on + strand, within EX31_RS19350at 496.343 kb on - strand, within EX31_RS19350at 496.455 kb on + strand, within EX31_RS19350at 496.456 kb on - strand, within EX31_RS19350at 496.660 kb on + strand, within EX31_RS19350at 496.660 kb on + strand, within EX31_RS19350at 496.660 kb on + strand, within EX31_RS19350at 496.660 kb on + strand, within EX31_RS19350at 496.661 kb on - strand, within EX31_RS19350at 496.661 kb on - strand, within EX31_RS19350at 496.725 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking
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494,428 - EX31_RS19340 0.31 -1.1
494,649 + EX31_RS19340 0.53 -0.4
494,650 - EX31_RS19340 0.53 +0.3
494,650 - EX31_RS19340 0.53 +1.1
494,650 - EX31_RS19340 0.53 +0.1
494,650 - EX31_RS19340 0.53 -0.9
494,650 - EX31_RS19340 0.53 -0.2
494,750 + EX31_RS19340 0.63 -0.8
494,821 + EX31_RS19340 0.70 -1.1
494,863 + EX31_RS19340 0.74 -0.5
494,863 + EX31_RS19340 0.74 -0.5
494,967 + EX31_RS19340 0.85 -0.4
494,967 + EX31_RS19340 0.85 +0.3
494,968 - EX31_RS19340 0.85 +1.1
494,968 - EX31_RS19340 0.85 -1.1
494,968 - EX31_RS19340 0.85 -0.1
495,096 - -1.6
495,122 - -0.4
495,128 + -0.4
495,129 - +0.4
495,129 - -0.2
495,129 - +0.4
495,205 + +1.3
495,205 + -0.5
495,205 + -1.3
495,205 + -0.1
495,205 + +0.3
495,205 + +0.5
495,205 + +0.3
495,206 - EX31_RS19345 0.10 +0.9
495,206 - EX31_RS19345 0.10 +2.1
495,206 - EX31_RS19345 0.10 -0.3
495,206 - EX31_RS19345 0.10 -1.4
495,366 + EX31_RS19345 0.29 +1.7
495,366 + EX31_RS19345 0.29 -0.0
495,366 + EX31_RS19345 0.29 -1.1
495,367 - EX31_RS19345 0.29 -1.3
495,367 - EX31_RS19345 0.29 -0.9
495,367 - EX31_RS19345 0.29 -0.4
495,410 - EX31_RS19345 0.34 +1.2
495,410 - EX31_RS19345 0.34 -0.3
495,468 - EX31_RS19345 0.41 -0.8
495,468 - EX31_RS19345 0.41 -0.0
495,468 - EX31_RS19345 0.41 +0.1
495,469 + EX31_RS19345 0.41 +0.5
495,469 + EX31_RS19345 0.41 +0.6
495,469 + EX31_RS19345 0.41 +0.5
495,469 + EX31_RS19345 0.41 -0.3
495,470 - EX31_RS19345 0.41 -0.4
495,470 - EX31_RS19345 0.41 +1.2
495,470 - EX31_RS19345 0.41 +0.2
495,470 - EX31_RS19345 0.41 -0.6
495,470 - EX31_RS19345 0.41 -1.3
495,470 - EX31_RS19345 0.41 -1.0
495,470 - EX31_RS19345 0.41 -0.1
495,470 - EX31_RS19345 0.41 -0.9
495,470 - EX31_RS19345 0.41 +1.1
495,470 - EX31_RS19345 0.41 -0.3
495,470 - EX31_RS19345 0.41 +0.2
495,470 - EX31_RS19345 0.41 +0.9
495,470 - EX31_RS19345 0.41 -0.3
495,533 + EX31_RS19345 0.48 -1.6
495,563 + EX31_RS19345 0.52 -0.6
495,563 + EX31_RS19345 0.52 +0.3
495,564 - EX31_RS19345 0.52 -0.7
495,564 - EX31_RS19345 0.52 -1.2
495,564 - EX31_RS19345 0.52 -0.4
495,564 - EX31_RS19345 0.52 -0.4
495,564 - EX31_RS19345 0.52 +0.0
495,872 + EX31_RS19345 0.88 +0.3
495,905 + +0.4
495,906 - +0.6
495,906 - -0.5
495,906 - -0.1
495,924 - +0.3
495,924 - -2.5
495,949 + +0.1
495,970 + -0.4
495,971 - -1.5
496,005 + +0.5
496,005 + -1.1
496,005 + +1.2
496,006 - +0.2
496,008 + +0.5
496,008 + +0.1
496,008 + +0.6
496,084 + EX31_RS19350 0.15 +1.5
496,084 + EX31_RS19350 0.15 -0.1
496,117 - EX31_RS19350 0.19 -0.9
496,183 + EX31_RS19350 0.27 +0.1
496,184 - EX31_RS19350 0.28 +1.6
496,342 + EX31_RS19350 0.47 -0.4
496,342 + EX31_RS19350 0.47 +0.6
496,343 - EX31_RS19350 0.47 -1.3
496,455 + EX31_RS19350 0.62 +2.3
496,456 - EX31_RS19350 0.62 +0.4
496,660 + EX31_RS19350 0.87 +0.8
496,660 + EX31_RS19350 0.87 -1.9
496,660 + EX31_RS19350 0.87 +0.7
496,660 + EX31_RS19350 0.87 +0.7
496,661 - EX31_RS19350 0.87 -1.9
496,661 - EX31_RS19350 0.87 +0.5
496,725 + -1.4

Or see this region's nucleotide sequence