Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS19335 and EX31_RS19340 overlap by 16 nucleotides EX31_RS19340 and EX31_RS19345 overlap by 1 nucleotides EX31_RS19345 and EX31_RS19350 overlap by 14 nucleotides
EX31_RS19335: EX31_RS19335 - o-succinylbenzoate--CoA ligase, at 492,761 to 494,134
_RS19335
EX31_RS19340: EX31_RS19340 - o-succinylbenzoate synthase, at 494,119 to 495,120
_RS19340
EX31_RS19345: EX31_RS19345 - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 495,120 to 495,977
_RS19345
EX31_RS19350: EX31_RS19350 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 495,964 to 496,761
_RS19350
Position (kb) 494
495
496 Strain fitness (log2 ratio) -2
-1
0
1
2 at 493.157 kb on + strand, within EX31_RS19335 at 493.158 kb on - strand, within EX31_RS19335 at 493.158 kb on - strand, within EX31_RS19335 at 493.158 kb on - strand, within EX31_RS19335 at 493.177 kb on - strand, within EX31_RS19335 at 493.189 kb on + strand, within EX31_RS19335 at 493.189 kb on + strand, within EX31_RS19335 at 493.190 kb on - strand, within EX31_RS19335 at 493.247 kb on + strand, within EX31_RS19335 at 493.247 kb on + strand, within EX31_RS19335 at 493.334 kb on + strand, within EX31_RS19335 at 493.334 kb on + strand, within EX31_RS19335 at 493.335 kb on - strand, within EX31_RS19335 at 493.335 kb on - strand, within EX31_RS19335 at 493.589 kb on + strand, within EX31_RS19335 at 493.597 kb on + strand, within EX31_RS19335 at 493.597 kb on + strand, within EX31_RS19335 at 493.597 kb on + strand, within EX31_RS19335 at 493.603 kb on + strand, within EX31_RS19335 at 493.663 kb on + strand, within EX31_RS19335 at 493.663 kb on + strand, within EX31_RS19335 at 493.663 kb on + strand, within EX31_RS19335 at 493.663 kb on + strand, within EX31_RS19335 at 493.664 kb on - strand, within EX31_RS19335 at 493.664 kb on - strand, within EX31_RS19335 at 493.720 kb on + strand, within EX31_RS19335 at 493.783 kb on + strand, within EX31_RS19335 at 493.817 kb on + strand, within EX31_RS19335 at 493.856 kb on + strand, within EX31_RS19335 at 493.856 kb on + strand, within EX31_RS19335 at 493.856 kb on + strand, within EX31_RS19335 at 493.856 kb on + strand, within EX31_RS19335 at 493.857 kb on - strand, within EX31_RS19335 at 493.857 kb on - strand, within EX31_RS19335 at 493.857 kb on - strand, within EX31_RS19335 at 493.857 kb on - strand, within EX31_RS19335 at 493.857 kb on - strand, within EX31_RS19335 at 493.908 kb on - strand, within EX31_RS19335 at 493.921 kb on - strand, within EX31_RS19335 at 494.027 kb on + strand at 494.050 kb on + strand at 494.050 kb on + strand at 494.051 kb on - strand at 494.428 kb on - strand, within EX31_RS19340 at 494.649 kb on + strand, within EX31_RS19340 at 494.650 kb on - strand, within EX31_RS19340 at 494.650 kb on - strand, within EX31_RS19340 at 494.650 kb on - strand, within EX31_RS19340 at 494.650 kb on - strand, within EX31_RS19340 at 494.650 kb on - strand, within EX31_RS19340 at 494.750 kb on + strand, within EX31_RS19340 at 494.821 kb on + strand, within EX31_RS19340 at 494.863 kb on + strand, within EX31_RS19340 at 494.863 kb on + strand, within EX31_RS19340 at 494.967 kb on + strand, within EX31_RS19340 at 494.967 kb on + strand, within EX31_RS19340 at 494.968 kb on - strand, within EX31_RS19340 at 494.968 kb on - strand, within EX31_RS19340 at 494.968 kb on - strand, within EX31_RS19340 at 495.096 kb on - strand at 495.122 kb on - strand at 495.128 kb on + strand at 495.129 kb on - strand at 495.129 kb on - strand at 495.129 kb on - strand at 495.205 kb on + strand at 495.205 kb on + strand at 495.205 kb on + strand at 495.205 kb on + strand at 495.205 kb on + strand at 495.205 kb on + strand at 495.205 kb on + strand at 495.206 kb on - strand, within EX31_RS19345 at 495.206 kb on - strand, within EX31_RS19345 at 495.206 kb on - strand, within EX31_RS19345 at 495.206 kb on - strand, within EX31_RS19345 at 495.366 kb on + strand, within EX31_RS19345 at 495.366 kb on + strand, within EX31_RS19345 at 495.366 kb on + strand, within EX31_RS19345 at 495.367 kb on - strand, within EX31_RS19345 at 495.367 kb on - strand, within EX31_RS19345 at 495.367 kb on - strand, within EX31_RS19345 at 495.410 kb on - strand, within EX31_RS19345 at 495.410 kb on - strand, within EX31_RS19345 at 495.468 kb on - strand, within EX31_RS19345 at 495.468 kb on - strand, within EX31_RS19345 at 495.468 kb on - strand, within EX31_RS19345 at 495.469 kb on + strand, within EX31_RS19345 at 495.469 kb on + strand, within EX31_RS19345 at 495.469 kb on + strand, within EX31_RS19345 at 495.469 kb on + strand, within EX31_RS19345 at 495.470 kb on - strand, within EX31_RS19345 at 495.470 kb on - strand, within EX31_RS19345 at 495.470 kb on - strand, within EX31_RS19345 at 495.470 kb on - strand, within EX31_RS19345 at 495.470 kb on - strand, within EX31_RS19345 at 495.470 kb on - strand, within EX31_RS19345 at 495.470 kb on - strand, within EX31_RS19345 at 495.470 kb on - strand, within EX31_RS19345 at 495.470 kb on - strand, within EX31_RS19345 at 495.470 kb on - strand, within EX31_RS19345 at 495.470 kb on - strand, within EX31_RS19345 at 495.470 kb on - strand, within EX31_RS19345 at 495.470 kb on - strand, within EX31_RS19345 at 495.533 kb on + strand, within EX31_RS19345 at 495.563 kb on + strand, within EX31_RS19345 at 495.563 kb on + strand, within EX31_RS19345 at 495.564 kb on - strand, within EX31_RS19345 at 495.564 kb on - strand, within EX31_RS19345 at 495.564 kb on - strand, within EX31_RS19345 at 495.564 kb on - strand, within EX31_RS19345 at 495.564 kb on - strand, within EX31_RS19345 at 495.872 kb on + strand, within EX31_RS19345 at 495.905 kb on + strand at 495.906 kb on - strand at 495.906 kb on - strand at 495.906 kb on - strand at 495.924 kb on - strand at 495.924 kb on - strand at 495.949 kb on + strand at 495.970 kb on + strand at 495.971 kb on - strand at 496.005 kb on + strand at 496.005 kb on + strand at 496.005 kb on + strand at 496.006 kb on - strand at 496.008 kb on + strand at 496.008 kb on + strand at 496.008 kb on + strand at 496.084 kb on + strand, within EX31_RS19350 at 496.084 kb on + strand, within EX31_RS19350 at 496.117 kb on - strand, within EX31_RS19350
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 493,157 + EX31_RS19335 0.29 +1.3 493,158 - EX31_RS19335 0.29 +0.7 493,158 - EX31_RS19335 0.29 -0.2 493,158 - EX31_RS19335 0.29 -0.3 493,177 - EX31_RS19335 0.30 +0.0 493,189 + EX31_RS19335 0.31 -0.2 493,189 + EX31_RS19335 0.31 +0.8 493,190 - EX31_RS19335 0.31 -1.3 493,247 + EX31_RS19335 0.35 -0.5 493,247 + EX31_RS19335 0.35 -1.4 493,334 + EX31_RS19335 0.42 +0.5 493,334 + EX31_RS19335 0.42 -1.5 493,335 - EX31_RS19335 0.42 +0.7 493,335 - EX31_RS19335 0.42 +0.2 493,589 + EX31_RS19335 0.60 -0.6 493,597 + EX31_RS19335 0.61 +0.7 493,597 + EX31_RS19335 0.61 -2.4 493,597 + EX31_RS19335 0.61 -0.6 493,603 + EX31_RS19335 0.61 +0.1 493,663 + EX31_RS19335 0.66 +0.4 493,663 + EX31_RS19335 0.66 +0.1 493,663 + EX31_RS19335 0.66 +0.7 493,663 + EX31_RS19335 0.66 +1.6 493,664 - EX31_RS19335 0.66 +0.8 493,664 - EX31_RS19335 0.66 +1.8 493,720 + EX31_RS19335 0.70 +0.5 493,783 + EX31_RS19335 0.74 +0.1 493,817 + EX31_RS19335 0.77 -0.8 493,856 + EX31_RS19335 0.80 +0.0 493,856 + EX31_RS19335 0.80 +0.5 493,856 + EX31_RS19335 0.80 -0.7 493,856 + EX31_RS19335 0.80 +0.5 493,857 - EX31_RS19335 0.80 -0.5 493,857 - EX31_RS19335 0.80 +1.1 493,857 - EX31_RS19335 0.80 -1.3 493,857 - EX31_RS19335 0.80 -0.8 493,857 - EX31_RS19335 0.80 -1.4 493,908 - EX31_RS19335 0.83 -0.1 493,921 - EX31_RS19335 0.84 -0.0 494,027 + +0.5 494,050 + +0.6 494,050 + +1.6 494,051 - -0.3 494,428 - EX31_RS19340 0.31 -1.1 494,649 + EX31_RS19340 0.53 -0.4 494,650 - EX31_RS19340 0.53 +0.3 494,650 - EX31_RS19340 0.53 +1.1 494,650 - EX31_RS19340 0.53 +0.1 494,650 - EX31_RS19340 0.53 -0.9 494,650 - EX31_RS19340 0.53 -0.2 494,750 + EX31_RS19340 0.63 -0.8 494,821 + EX31_RS19340 0.70 -1.1 494,863 + EX31_RS19340 0.74 -0.5 494,863 + EX31_RS19340 0.74 -0.5 494,967 + EX31_RS19340 0.85 -0.4 494,967 + EX31_RS19340 0.85 +0.3 494,968 - EX31_RS19340 0.85 +1.1 494,968 - EX31_RS19340 0.85 -1.1 494,968 - EX31_RS19340 0.85 -0.1 495,096 - -1.6 495,122 - -0.4 495,128 + -0.4 495,129 - +0.4 495,129 - -0.2 495,129 - +0.4 495,205 + +1.3 495,205 + -0.5 495,205 + -1.3 495,205 + -0.1 495,205 + +0.3 495,205 + +0.5 495,205 + +0.3 495,206 - EX31_RS19345 0.10 +0.9 495,206 - EX31_RS19345 0.10 +2.1 495,206 - EX31_RS19345 0.10 -0.3 495,206 - EX31_RS19345 0.10 -1.4 495,366 + EX31_RS19345 0.29 +1.7 495,366 + EX31_RS19345 0.29 -0.0 495,366 + EX31_RS19345 0.29 -1.1 495,367 - EX31_RS19345 0.29 -1.3 495,367 - EX31_RS19345 0.29 -0.9 495,367 - EX31_RS19345 0.29 -0.4 495,410 - EX31_RS19345 0.34 +1.2 495,410 - EX31_RS19345 0.34 -0.3 495,468 - EX31_RS19345 0.41 -0.8 495,468 - EX31_RS19345 0.41 -0.0 495,468 - EX31_RS19345 0.41 +0.1 495,469 + EX31_RS19345 0.41 +0.5 495,469 + EX31_RS19345 0.41 +0.6 495,469 + EX31_RS19345 0.41 +0.5 495,469 + EX31_RS19345 0.41 -0.3 495,470 - EX31_RS19345 0.41 -0.4 495,470 - EX31_RS19345 0.41 +1.2 495,470 - EX31_RS19345 0.41 +0.2 495,470 - EX31_RS19345 0.41 -0.6 495,470 - EX31_RS19345 0.41 -1.3 495,470 - EX31_RS19345 0.41 -1.0 495,470 - EX31_RS19345 0.41 -0.1 495,470 - EX31_RS19345 0.41 -0.9 495,470 - EX31_RS19345 0.41 +1.1 495,470 - EX31_RS19345 0.41 -0.3 495,470 - EX31_RS19345 0.41 +0.2 495,470 - EX31_RS19345 0.41 +0.9 495,470 - EX31_RS19345 0.41 -0.3 495,533 + EX31_RS19345 0.48 -1.6 495,563 + EX31_RS19345 0.52 -0.6 495,563 + EX31_RS19345 0.52 +0.3 495,564 - EX31_RS19345 0.52 -0.7 495,564 - EX31_RS19345 0.52 -1.2 495,564 - EX31_RS19345 0.52 -0.4 495,564 - EX31_RS19345 0.52 -0.4 495,564 - EX31_RS19345 0.52 +0.0 495,872 + EX31_RS19345 0.88 +0.3 495,905 + +0.4 495,906 - +0.6 495,906 - -0.5 495,906 - -0.1 495,924 - +0.3 495,924 - -2.5 495,949 + +0.1 495,970 + -0.4 495,971 - -1.5 496,005 + +0.5 496,005 + -1.1 496,005 + +1.2 496,006 - +0.2 496,008 + +0.5 496,008 + +0.1 496,008 + +0.6 496,084 + EX31_RS19350 0.15 +1.5 496,084 + EX31_RS19350 0.15 -0.1 496,117 - EX31_RS19350 0.19 -0.9
Or see this region's nucleotide sequence