Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS19200 and EX31_RS19205 are separated by 160 nucleotides EX31_RS19205 and EX31_RS19210 are separated by 216 nucleotides EX31_RS19210 and EX31_RS19215 are separated by 322 nucleotides EX31_RS19215 and EX31_RS19220 are separated by 24 nucleotides
EX31_RS19200: EX31_RS19200 - DEAD/DEAH box helicase, at 436,119 to 437,882
_RS19200
EX31_RS19205: EX31_RS19205 - 50S ribosomal protein L25, at 438,043 to 438,333
_RS19205
EX31_RS19210: EX31_RS19210 - nucleoid-associated protein YejK, at 438,550 to 439,554
_RS19210
EX31_RS19215: EX31_RS19215 - YejL family protein, at 439,877 to 440,104
_RS19215
EX31_RS19220: EX31_RS19220 - DUF3413 domain-containing protein, at 440,129 to 441,910
_RS19220
Position (kb) 438
439
440 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 437.555 kb on + strand, within EX31_RS19200 at 437.556 kb on - strand, within EX31_RS19200 at 437.556 kb on - strand, within EX31_RS19200 at 437.603 kb on + strand, within EX31_RS19200 at 437.603 kb on + strand, within EX31_RS19200 at 437.603 kb on + strand, within EX31_RS19200 at 437.604 kb on - strand, within EX31_RS19200 at 437.607 kb on + strand, within EX31_RS19200 at 437.607 kb on + strand, within EX31_RS19200 at 437.607 kb on + strand, within EX31_RS19200 at 437.607 kb on + strand, within EX31_RS19200 at 437.607 kb on + strand, within EX31_RS19200 at 437.607 kb on + strand, within EX31_RS19200 at 437.608 kb on - strand, within EX31_RS19200 at 437.608 kb on - strand, within EX31_RS19200 at 437.608 kb on - strand, within EX31_RS19200 at 437.608 kb on - strand, within EX31_RS19200 at 437.611 kb on - strand, within EX31_RS19200 at 437.611 kb on - strand, within EX31_RS19200 at 437.611 kb on - strand, within EX31_RS19200 at 437.684 kb on + strand, within EX31_RS19200 at 437.767 kb on + strand at 437.768 kb on - strand at 437.841 kb on + strand at 437.841 kb on + strand at 437.842 kb on - strand at 437.905 kb on + strand at 437.906 kb on - strand at 437.939 kb on + strand at 438.020 kb on + strand at 438.021 kb on - strand at 438.021 kb on - strand at 438.026 kb on + strand at 438.039 kb on + strand at 438.039 kb on + strand at 438.039 kb on + strand at 438.039 kb on + strand at 438.040 kb on - strand at 438.040 kb on - strand at 438.040 kb on - strand at 438.040 kb on - strand at 438.040 kb on - strand at 438.051 kb on + strand at 438.052 kb on - strand at 438.052 kb on - strand at 438.059 kb on + strand at 438.059 kb on + strand at 438.059 kb on + strand at 438.059 kb on + strand at 438.059 kb on + strand at 438.065 kb on + strand at 438.065 kb on + strand at 438.065 kb on + strand at 438.065 kb on + strand at 438.132 kb on + strand, within EX31_RS19205 at 438.165 kb on + strand, within EX31_RS19205 at 438.217 kb on + strand, within EX31_RS19205 at 438.231 kb on + strand, within EX31_RS19205 at 438.231 kb on + strand, within EX31_RS19205 at 438.231 kb on + strand, within EX31_RS19205 at 438.232 kb on - strand, within EX31_RS19205 at 438.278 kb on + strand, within EX31_RS19205 at 438.414 kb on + strand at 438.428 kb on - strand at 438.436 kb on + strand at 438.437 kb on - strand at 438.437 kb on - strand at 438.483 kb on + strand at 438.483 kb on + strand at 438.483 kb on + strand at 438.483 kb on + strand at 438.483 kb on + strand at 438.483 kb on + strand at 438.483 kb on + strand at 438.483 kb on + strand at 438.484 kb on - strand at 438.484 kb on - strand at 438.484 kb on - strand at 438.484 kb on - strand at 438.484 kb on - strand at 438.484 kb on - strand at 438.487 kb on + strand at 438.487 kb on + strand at 438.488 kb on - strand at 438.488 kb on - strand at 438.488 kb on - strand at 438.488 kb on - strand at 438.488 kb on - strand at 438.488 kb on - strand at 438.488 kb on - strand at 438.488 kb on - strand at 438.488 kb on - strand at 438.491 kb on + strand at 438.491 kb on + strand at 438.491 kb on + strand at 438.491 kb on + strand at 438.491 kb on + strand at 438.491 kb on + strand at 438.491 kb on + strand at 438.491 kb on + strand at 438.491 kb on + strand at 438.491 kb on + strand at 438.491 kb on + strand at 438.491 kb on + strand at 438.492 kb on - strand at 438.492 kb on - strand at 438.492 kb on - strand at 438.492 kb on - strand at 438.492 kb on - strand at 438.492 kb on - strand at 438.492 kb on - strand at 438.492 kb on - strand at 438.492 kb on - strand at 438.492 kb on - strand at 438.539 kb on + strand at 438.539 kb on + strand at 438.540 kb on - strand at 438.551 kb on + strand at 438.551 kb on + strand at 438.552 kb on - strand at 438.552 kb on - strand at 438.552 kb on - strand at 438.552 kb on - strand at 438.555 kb on + strand at 438.556 kb on - strand at 438.556 kb on - strand at 438.575 kb on - strand at 438.575 kb on - strand at 438.575 kb on - strand at 438.575 kb on - strand at 438.619 kb on - strand at 438.619 kb on - strand at 438.759 kb on - strand, within EX31_RS19210 at 438.759 kb on - strand, within EX31_RS19210 at 438.775 kb on - strand, within EX31_RS19210 at 438.869 kb on + strand, within EX31_RS19210 at 438.869 kb on + strand, within EX31_RS19210 at 438.870 kb on - strand, within EX31_RS19210 at 438.870 kb on - strand, within EX31_RS19210 at 438.870 kb on - strand, within EX31_RS19210 at 438.870 kb on - strand, within EX31_RS19210 at 438.870 kb on - strand, within EX31_RS19210 at 438.873 kb on + strand, within EX31_RS19210 at 438.873 kb on + strand, within EX31_RS19210 at 438.873 kb on + strand, within EX31_RS19210 at 438.874 kb on - strand, within EX31_RS19210 at 438.874 kb on - strand, within EX31_RS19210 at 438.874 kb on - strand, within EX31_RS19210 at 438.874 kb on - strand, within EX31_RS19210 at 438.874 kb on - strand, within EX31_RS19210 at 438.874 kb on - strand, within EX31_RS19210 at 438.874 kb on - strand, within EX31_RS19210 at 438.874 kb on - strand, within EX31_RS19210 at 438.875 kb on + strand, within EX31_RS19210 at 438.875 kb on + strand, within EX31_RS19210 at 438.875 kb on + strand, within EX31_RS19210 at 438.875 kb on + strand, within EX31_RS19210 at 438.876 kb on - strand, within EX31_RS19210 at 438.876 kb on - strand, within EX31_RS19210 at 438.876 kb on - strand, within EX31_RS19210 at 438.876 kb on - strand, within EX31_RS19210 at 438.890 kb on + strand, within EX31_RS19210 at 438.891 kb on - strand, within EX31_RS19210 at 438.891 kb on - strand, within EX31_RS19210 at 438.891 kb on - strand, within EX31_RS19210 at 438.891 kb on - strand, within EX31_RS19210 at 438.891 kb on - strand, within EX31_RS19210 at 438.928 kb on - strand, within EX31_RS19210 at 439.102 kb on - strand, within EX31_RS19210 at 439.102 kb on - strand, within EX31_RS19210 at 439.136 kb on + strand, within EX31_RS19210 at 439.137 kb on - strand, within EX31_RS19210 at 439.177 kb on - strand, within EX31_RS19210 at 439.207 kb on + strand, within EX31_RS19210 at 439.208 kb on - strand, within EX31_RS19210 at 439.208 kb on - strand, within EX31_RS19210 at 439.211 kb on + strand, within EX31_RS19210 at 439.212 kb on - strand, within EX31_RS19210 at 439.212 kb on - strand, within EX31_RS19210 at 439.395 kb on - strand, within EX31_RS19210 at 439.395 kb on - strand, within EX31_RS19210 at 439.395 kb on - strand, within EX31_RS19210 at 439.415 kb on + strand, within EX31_RS19210 at 439.415 kb on + strand, within EX31_RS19210 at 439.415 kb on + strand, within EX31_RS19210 at 439.416 kb on - strand, within EX31_RS19210 at 439.416 kb on - strand, within EX31_RS19210 at 439.473 kb on + strand at 439.474 kb on - strand at 439.587 kb on + strand at 439.588 kb on - strand at 439.659 kb on + strand at 439.660 kb on - strand at 439.660 kb on - strand at 439.660 kb on - strand at 439.689 kb on + strand at 439.745 kb on - strand at 439.831 kb on + strand at 439.836 kb on + strand at 439.871 kb on + strand at 439.871 kb on + strand at 439.871 kb on + strand at 439.871 kb on + strand at 439.871 kb on + strand at 439.871 kb on + strand at 439.871 kb on + strand at 439.871 kb on + strand at 439.876 kb on + strand at 439.876 kb on + strand at 439.876 kb on + strand at 439.895 kb on + strand at 439.993 kb on + strand, within EX31_RS19215 at 439.993 kb on + strand, within EX31_RS19215 at 440.008 kb on + strand, within EX31_RS19215 at 440.008 kb on + strand, within EX31_RS19215 at 440.011 kb on + strand, within EX31_RS19215 at 440.011 kb on + strand, within EX31_RS19215 at 440.014 kb on + strand, within EX31_RS19215 at 440.082 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 437,555 + EX31_RS19200 0.81 -0.1 437,556 - EX31_RS19200 0.81 -0.3 437,556 - EX31_RS19200 0.81 -1.4 437,603 + EX31_RS19200 0.84 +0.7 437,603 + EX31_RS19200 0.84 -0.3 437,603 + EX31_RS19200 0.84 -0.6 437,604 - EX31_RS19200 0.84 -1.9 437,607 + EX31_RS19200 0.84 +0.2 437,607 + EX31_RS19200 0.84 +0.1 437,607 + EX31_RS19200 0.84 -0.0 437,607 + EX31_RS19200 0.84 +0.9 437,607 + EX31_RS19200 0.84 -1.3 437,607 + EX31_RS19200 0.84 -0.3 437,608 - EX31_RS19200 0.84 +0.2 437,608 - EX31_RS19200 0.84 +2.1 437,608 - EX31_RS19200 0.84 +2.5 437,608 - EX31_RS19200 0.84 +0.2 437,611 - EX31_RS19200 0.85 -2.2 437,611 - EX31_RS19200 0.85 +0.5 437,611 - EX31_RS19200 0.85 -0.5 437,684 + EX31_RS19200 0.89 +0.9 437,767 + +0.7 437,768 - +0.7 437,841 + +0.9 437,841 + -1.1 437,842 - -0.9 437,905 + +1.2 437,906 - +2.5 437,939 + +0.1 438,020 + -0.4 438,021 - +2.1 438,021 - -0.5 438,026 + +0.4 438,039 + -1.1 438,039 + -0.8 438,039 + -0.2 438,039 + -1.3 438,040 - +0.6 438,040 - -0.8 438,040 - -1.5 438,040 - -1.9 438,040 - +1.1 438,051 + +1.0 438,052 - -1.3 438,052 - -1.7 438,059 + -0.9 438,059 + +1.1 438,059 + -2.8 438,059 + +0.3 438,059 + -1.0 438,065 + -0.2 438,065 + -2.9 438,065 + -1.3 438,065 + -2.4 438,132 + EX31_RS19205 0.31 -1.8 438,165 + EX31_RS19205 0.42 -2.6 438,217 + EX31_RS19205 0.60 -0.7 438,231 + EX31_RS19205 0.65 -1.4 438,231 + EX31_RS19205 0.65 +0.3 438,231 + EX31_RS19205 0.65 -0.5 438,232 - EX31_RS19205 0.65 -1.3 438,278 + EX31_RS19205 0.81 -0.5 438,414 + -0.4 438,428 - +0.6 438,436 + -2.3 438,437 - +0.4 438,437 - -0.6 438,483 + -0.9 438,483 + -0.7 438,483 + +0.5 438,483 + +0.8 438,483 + -0.7 438,483 + +0.3 438,483 + -0.2 438,483 + +0.8 438,484 - +0.9 438,484 - -1.3 438,484 - +2.5 438,484 - -0.7 438,484 - -0.4 438,484 - +0.0 438,487 + +0.6 438,487 + +0.3 438,488 - -1.0 438,488 - +0.0 438,488 - +0.1 438,488 - -1.1 438,488 - +1.1 438,488 - -0.5 438,488 - +0.1 438,488 - +0.3 438,488 - -0.7 438,491 + +0.2 438,491 + -0.5 438,491 + +1.6 438,491 + -0.3 438,491 + +0.6 438,491 + -0.3 438,491 + -0.5 438,491 + +2.1 438,491 + -0.3 438,491 + +0.2 438,491 + +0.3 438,491 + +0.7 438,492 - +1.8 438,492 - -0.2 438,492 - +0.5 438,492 - +0.2 438,492 - -0.0 438,492 - +0.8 438,492 - +1.5 438,492 - +0.8 438,492 - +2.1 438,492 - +0.3 438,539 + +0.9 438,539 + +1.1 438,540 - -0.4 438,551 + +1.2 438,551 + -0.2 438,552 - +0.5 438,552 - -0.2 438,552 - +0.2 438,552 - +0.3 438,555 + -0.7 438,556 - +1.1 438,556 - +1.1 438,575 - -0.1 438,575 - +0.3 438,575 - +0.8 438,575 - +0.8 438,619 - +0.1 438,619 - -1.5 438,759 - EX31_RS19210 0.21 -1.5 438,759 - EX31_RS19210 0.21 +2.1 438,775 - EX31_RS19210 0.22 -1.4 438,869 + EX31_RS19210 0.32 -1.4 438,869 + EX31_RS19210 0.32 +2.1 438,870 - EX31_RS19210 0.32 +0.1 438,870 - EX31_RS19210 0.32 -0.8 438,870 - EX31_RS19210 0.32 +0.3 438,870 - EX31_RS19210 0.32 -0.7 438,870 - EX31_RS19210 0.32 +1.3 438,873 + EX31_RS19210 0.32 +1.1 438,873 + EX31_RS19210 0.32 +0.2 438,873 + EX31_RS19210 0.32 -0.3 438,874 - EX31_RS19210 0.32 -0.3 438,874 - EX31_RS19210 0.32 -0.7 438,874 - EX31_RS19210 0.32 +0.7 438,874 - EX31_RS19210 0.32 -0.1 438,874 - EX31_RS19210 0.32 -0.9 438,874 - EX31_RS19210 0.32 +0.8 438,874 - EX31_RS19210 0.32 +1.3 438,874 - EX31_RS19210 0.32 -0.9 438,875 + EX31_RS19210 0.32 -0.1 438,875 + EX31_RS19210 0.32 -0.2 438,875 + EX31_RS19210 0.32 +0.3 438,875 + EX31_RS19210 0.32 -1.5 438,876 - EX31_RS19210 0.32 +0.4 438,876 - EX31_RS19210 0.32 -0.6 438,876 - EX31_RS19210 0.32 -0.0 438,876 - EX31_RS19210 0.32 -0.5 438,890 + EX31_RS19210 0.34 +0.1 438,891 - EX31_RS19210 0.34 +1.4 438,891 - EX31_RS19210 0.34 -1.7 438,891 - EX31_RS19210 0.34 -0.2 438,891 - EX31_RS19210 0.34 -0.2 438,891 - EX31_RS19210 0.34 +0.1 438,928 - EX31_RS19210 0.38 -0.1 439,102 - EX31_RS19210 0.55 -0.7 439,102 - EX31_RS19210 0.55 +1.9 439,136 + EX31_RS19210 0.58 +0.1 439,137 - EX31_RS19210 0.58 -0.6 439,177 - EX31_RS19210 0.62 +0.4 439,207 + EX31_RS19210 0.65 -0.9 439,208 - EX31_RS19210 0.65 -1.0 439,208 - EX31_RS19210 0.65 +0.1 439,211 + EX31_RS19210 0.66 +1.1 439,212 - EX31_RS19210 0.66 +0.2 439,212 - EX31_RS19210 0.66 +0.3 439,395 - EX31_RS19210 0.84 -0.9 439,395 - EX31_RS19210 0.84 +1.6 439,395 - EX31_RS19210 0.84 +0.6 439,415 + EX31_RS19210 0.86 -0.4 439,415 + EX31_RS19210 0.86 -0.7 439,415 + EX31_RS19210 0.86 +0.7 439,416 - EX31_RS19210 0.86 -0.2 439,416 - EX31_RS19210 0.86 +0.8 439,473 + -0.3 439,474 - -0.5 439,587 + -0.8 439,588 - -0.3 439,659 + +0.1 439,660 - +1.1 439,660 - -0.5 439,660 - -0.6 439,689 + -0.5 439,745 - +0.2 439,831 + +1.8 439,836 + -2.4 439,871 + +0.2 439,871 + -0.8 439,871 + -0.1 439,871 + -1.8 439,871 + +0.6 439,871 + -0.6 439,871 + +0.4 439,871 + +0.5 439,876 + -0.3 439,876 + +0.5 439,876 + -0.7 439,895 + +0.0 439,993 + EX31_RS19215 0.51 +1.6 439,993 + EX31_RS19215 0.51 +0.7 440,008 + EX31_RS19215 0.57 +0.1 440,008 + EX31_RS19215 0.57 +0.4 440,011 + EX31_RS19215 0.59 +0.4 440,011 + EX31_RS19215 0.59 -0.6 440,014 + EX31_RS19215 0.60 +0.9 440,082 + +0.9
Or see this region's nucleotide sequence