Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18345 and EX31_RS18350 overlap by 8 nucleotides EX31_RS18350 and EX31_RS18355 are separated by 89 nucleotides EX31_RS18355 and EX31_RS18360 overlap by 4 nucleotides EX31_RS18360 and EX31_RS18365 overlap by 17 nucleotides EX31_RS18365 and EX31_RS18370 are separated by 2 nucleotides EX31_RS18370 and EX31_RS18375 overlap by 1 nucleotides
EX31_RS18345: EX31_RS18345 - phage virion morphogenesis protein, at 242,409 to 242,855
_RS18345
EX31_RS18350: EX31_RS18350 - phage tail protein, at 242,848 to 243,315
_RS18350
EX31_RS18355: EX31_RS18355 - LysB family phage lysis regulatory protein, at 243,405 to 243,827
_RS18355
EX31_RS18360: EX31_RS18360 - lysozyme, at 243,824 to 244,333
_RS18360
EX31_RS18365: EX31_RS18365 - hypothetical protein, at 244,317 to 244,526
_RS18365
EX31_RS18370: EX31_RS18370 - tail protein X, at 244,529 to 244,732
_RS18370
EX31_RS18375: EX31_RS18375 - head completion/stabilization protein, at 244,732 to 245,226
_RS18375
Position (kb) 243
244 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 242.489 kb on - strand, within EX31_RS18345 at 242.501 kb on + strand, within EX31_RS18345 at 242.501 kb on + strand, within EX31_RS18345 at 242.652 kb on + strand, within EX31_RS18345 at 242.652 kb on + strand, within EX31_RS18345 at 242.652 kb on + strand, within EX31_RS18345 at 242.653 kb on - strand, within EX31_RS18345 at 242.653 kb on - strand, within EX31_RS18345 at 242.653 kb on - strand, within EX31_RS18345 at 242.698 kb on + strand, within EX31_RS18345 at 242.885 kb on + strand at 242.885 kb on + strand at 242.885 kb on + strand at 242.885 kb on + strand at 242.885 kb on + strand at 242.885 kb on + strand at 242.885 kb on + strand at 242.885 kb on + strand at 242.886 kb on - strand at 242.886 kb on - strand at 242.886 kb on - strand at 242.886 kb on - strand at 242.886 kb on - strand at 242.886 kb on - strand at 242.886 kb on - strand at 243.024 kb on + strand, within EX31_RS18350 at 243.027 kb on + strand, within EX31_RS18350 at 243.027 kb on + strand, within EX31_RS18350 at 243.027 kb on + strand, within EX31_RS18350 at 243.027 kb on + strand, within EX31_RS18350 at 243.028 kb on - strand, within EX31_RS18350 at 243.028 kb on - strand, within EX31_RS18350 at 243.030 kb on + strand, within EX31_RS18350 at 243.041 kb on + strand, within EX31_RS18350 at 243.041 kb on + strand, within EX31_RS18350 at 243.041 kb on + strand, within EX31_RS18350 at 243.042 kb on - strand, within EX31_RS18350 at 243.042 kb on - strand, within EX31_RS18350 at 243.042 kb on - strand, within EX31_RS18350 at 243.042 kb on - strand, within EX31_RS18350 at 243.133 kb on + strand, within EX31_RS18350 at 243.146 kb on + strand, within EX31_RS18350 at 243.146 kb on + strand, within EX31_RS18350 at 243.174 kb on - strand, within EX31_RS18350 at 243.214 kb on - strand, within EX31_RS18350 at 243.279 kb on + strand at 243.279 kb on + strand at 243.401 kb on + strand at 243.468 kb on + strand, within EX31_RS18355 at 243.581 kb on + strand, within EX31_RS18355 at 243.581 kb on + strand, within EX31_RS18355 at 243.582 kb on - strand, within EX31_RS18355 at 243.582 kb on - strand, within EX31_RS18355 at 243.721 kb on - strand, within EX31_RS18355 at 243.721 kb on - strand, within EX31_RS18355 at 243.750 kb on + strand, within EX31_RS18355 at 243.751 kb on - strand, within EX31_RS18355 at 243.751 kb on - strand, within EX31_RS18355 at 243.751 kb on - strand, within EX31_RS18355 at 243.804 kb on + strand at 243.820 kb on + strand at 243.820 kb on + strand at 243.820 kb on + strand at 243.821 kb on - strand at 243.821 kb on - strand at 243.821 kb on - strand at 243.821 kb on - strand at 243.821 kb on - strand at 243.821 kb on - strand at 243.821 kb on - strand at 243.852 kb on - strand at 243.903 kb on + strand, within EX31_RS18360 at 243.903 kb on + strand, within EX31_RS18360 at 243.903 kb on + strand, within EX31_RS18360 at 243.903 kb on + strand, within EX31_RS18360 at 243.903 kb on + strand, within EX31_RS18360 at 243.903 kb on + strand, within EX31_RS18360 at 243.903 kb on + strand, within EX31_RS18360 at 243.903 kb on + strand, within EX31_RS18360 at 243.903 kb on + strand, within EX31_RS18360 at 243.903 kb on + strand, within EX31_RS18360 at 243.903 kb on + strand, within EX31_RS18360 at 243.903 kb on + strand, within EX31_RS18360 at 243.904 kb on - strand, within EX31_RS18360 at 243.904 kb on - strand, within EX31_RS18360 at 243.904 kb on - strand, within EX31_RS18360 at 243.904 kb on - strand, within EX31_RS18360 at 243.960 kb on + strand, within EX31_RS18360 at 243.976 kb on + strand, within EX31_RS18360 at 244.026 kb on + strand, within EX31_RS18360 at 244.027 kb on - strand, within EX31_RS18360 at 244.027 kb on - strand, within EX31_RS18360 at 244.027 kb on - strand, within EX31_RS18360 at 244.027 kb on - strand, within EX31_RS18360 at 244.027 kb on - strand, within EX31_RS18360 at 244.061 kb on - strand, within EX31_RS18360 at 244.061 kb on - strand, within EX31_RS18360 at 244.123 kb on + strand, within EX31_RS18360 at 244.123 kb on + strand, within EX31_RS18360 at 244.375 kb on + strand, within EX31_RS18365 at 244.515 kb on + strand at 244.516 kb on - strand at 244.539 kb on - strand at 244.644 kb on - strand, within EX31_RS18370 at 244.677 kb on + strand, within EX31_RS18370 at 244.677 kb on + strand, within EX31_RS18370 at 244.678 kb on - strand, within EX31_RS18370 at 244.810 kb on + strand, within EX31_RS18375 at 244.810 kb on + strand, within EX31_RS18375
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 242,489 - EX31_RS18345 0.18 +0.7 242,501 + EX31_RS18345 0.21 +0.2 242,501 + EX31_RS18345 0.21 -0.2 242,652 + EX31_RS18345 0.54 -2.1 242,652 + EX31_RS18345 0.54 +0.2 242,652 + EX31_RS18345 0.54 +0.0 242,653 - EX31_RS18345 0.55 -1.4 242,653 - EX31_RS18345 0.55 -0.8 242,653 - EX31_RS18345 0.55 +0.6 242,698 + EX31_RS18345 0.65 +0.5 242,885 + -0.6 242,885 + -0.0 242,885 + -0.9 242,885 + +1.6 242,885 + -1.8 242,885 + -0.7 242,885 + +0.3 242,885 + +0.4 242,886 - +0.1 242,886 - -1.8 242,886 - +0.6 242,886 - +0.2 242,886 - -0.2 242,886 - -0.6 242,886 - +1.4 243,024 + EX31_RS18350 0.38 +0.6 243,027 + EX31_RS18350 0.38 -2.2 243,027 + EX31_RS18350 0.38 +0.1 243,027 + EX31_RS18350 0.38 -0.0 243,027 + EX31_RS18350 0.38 +0.4 243,028 - EX31_RS18350 0.38 +1.4 243,028 - EX31_RS18350 0.38 -1.0 243,030 + EX31_RS18350 0.39 -0.8 243,041 + EX31_RS18350 0.41 -0.6 243,041 + EX31_RS18350 0.41 +0.2 243,041 + EX31_RS18350 0.41 +0.5 243,042 - EX31_RS18350 0.41 +1.3 243,042 - EX31_RS18350 0.41 -0.9 243,042 - EX31_RS18350 0.41 -0.1 243,042 - EX31_RS18350 0.41 +0.0 243,133 + EX31_RS18350 0.61 +0.2 243,146 + EX31_RS18350 0.64 -0.3 243,146 + EX31_RS18350 0.64 +0.5 243,174 - EX31_RS18350 0.70 +0.4 243,214 - EX31_RS18350 0.78 -0.1 243,279 + -0.6 243,279 + +0.4 243,401 + +1.1 243,468 + EX31_RS18355 0.15 +0.7 243,581 + EX31_RS18355 0.42 +2.2 243,581 + EX31_RS18355 0.42 +1.2 243,582 - EX31_RS18355 0.42 -0.6 243,582 - EX31_RS18355 0.42 -0.1 243,721 - EX31_RS18355 0.75 +0.0 243,721 - EX31_RS18355 0.75 +0.5 243,750 + EX31_RS18355 0.82 +1.5 243,751 - EX31_RS18355 0.82 +0.6 243,751 - EX31_RS18355 0.82 -0.9 243,751 - EX31_RS18355 0.82 -0.8 243,804 + -1.0 243,820 + +0.3 243,820 + -1.6 243,820 + +2.0 243,821 - +0.8 243,821 - +0.4 243,821 - +1.2 243,821 - +0.4 243,821 - -0.4 243,821 - -0.5 243,821 - -1.1 243,852 - -0.3 243,903 + EX31_RS18360 0.15 -1.6 243,903 + EX31_RS18360 0.15 +3.3 243,903 + EX31_RS18360 0.15 +0.0 243,903 + EX31_RS18360 0.15 -2.0 243,903 + EX31_RS18360 0.15 +2.4 243,903 + EX31_RS18360 0.15 +0.2 243,903 + EX31_RS18360 0.15 -0.6 243,903 + EX31_RS18360 0.15 +0.4 243,903 + EX31_RS18360 0.15 -0.9 243,903 + EX31_RS18360 0.15 +1.4 243,903 + EX31_RS18360 0.15 +0.0 243,903 + EX31_RS18360 0.15 +1.2 243,904 - EX31_RS18360 0.16 +0.9 243,904 - EX31_RS18360 0.16 +0.4 243,904 - EX31_RS18360 0.16 -1.1 243,904 - EX31_RS18360 0.16 +0.0 243,960 + EX31_RS18360 0.27 -0.2 243,976 + EX31_RS18360 0.30 +0.3 244,026 + EX31_RS18360 0.40 +1.0 244,027 - EX31_RS18360 0.40 -0.2 244,027 - EX31_RS18360 0.40 +1.2 244,027 - EX31_RS18360 0.40 -1.4 244,027 - EX31_RS18360 0.40 +0.2 244,027 - EX31_RS18360 0.40 -0.2 244,061 - EX31_RS18360 0.46 -0.8 244,061 - EX31_RS18360 0.46 -2.2 244,123 + EX31_RS18360 0.59 +0.6 244,123 + EX31_RS18360 0.59 +0.7 244,375 + EX31_RS18365 0.28 -0.6 244,515 + +1.1 244,516 - -0.4 244,539 - -0.5 244,644 - EX31_RS18370 0.56 +0.4 244,677 + EX31_RS18370 0.73 +2.0 244,677 + EX31_RS18370 0.73 +0.4 244,678 - EX31_RS18370 0.73 -1.1 244,810 + EX31_RS18375 0.16 +2.0 244,810 + EX31_RS18375 0.16 -1.1
Or see this region's nucleotide sequence