Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18150 and EX31_RS18155 are separated by 51 nucleotides EX31_RS18155 and EX31_RS18160 are separated by 179 nucleotides EX31_RS18160 and EX31_RS18165 are separated by 361 nucleotides
EX31_RS18150: EX31_RS18150 - MBL fold metallo-hydrolase, at 195,475 to 196,122
_RS18150
EX31_RS18155: EX31_RS18155 - YcbK family protein, at 196,174 to 196,725
_RS18155
EX31_RS18160: EX31_RS18160 - L,D-transpeptidase, at 196,905 to 198,755
_RS18160
EX31_RS18165: EX31_RS18165 - chromosome partition protein MukB, at 199,117 to 203,565
_RS18165
Position (kb) 196
197
198
199 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 195.983 kb on - strand, within EX31_RS18150 at 196.060 kb on - strand at 196.104 kb on + strand at 196.105 kb on - strand at 196.107 kb on + strand at 196.107 kb on + strand at 196.107 kb on + strand at 196.108 kb on - strand at 196.108 kb on - strand at 196.163 kb on - strand at 196.163 kb on - strand at 196.167 kb on - strand at 196.167 kb on - strand at 196.167 kb on - strand at 196.215 kb on + strand at 196.216 kb on - strand at 196.229 kb on + strand at 196.230 kb on - strand, within EX31_RS18155 at 196.234 kb on + strand, within EX31_RS18155 at 196.235 kb on - strand, within EX31_RS18155 at 196.235 kb on - strand, within EX31_RS18155 at 196.235 kb on - strand, within EX31_RS18155 at 196.328 kb on + strand, within EX31_RS18155 at 196.328 kb on + strand, within EX31_RS18155 at 196.328 kb on + strand, within EX31_RS18155 at 196.328 kb on + strand, within EX31_RS18155 at 196.329 kb on - strand, within EX31_RS18155 at 196.329 kb on - strand, within EX31_RS18155 at 196.449 kb on - strand, within EX31_RS18155 at 196.594 kb on - strand, within EX31_RS18155 at 196.699 kb on - strand at 196.735 kb on - strand at 196.735 kb on - strand at 196.735 kb on - strand at 196.788 kb on + strand at 196.789 kb on - strand at 196.789 kb on - strand at 196.822 kb on + strand at 196.822 kb on + strand at 196.822 kb on + strand at 196.822 kb on + strand at 196.823 kb on - strand at 196.823 kb on - strand at 196.823 kb on - strand at 196.824 kb on + strand at 196.824 kb on + strand at 196.825 kb on - strand at 196.825 kb on - strand at 196.825 kb on - strand at 196.825 kb on - strand at 196.825 kb on - strand at 196.872 kb on - strand at 196.872 kb on - strand at 196.884 kb on - strand at 196.891 kb on + strand at 196.892 kb on - strand at 196.897 kb on + strand at 196.897 kb on + strand at 196.898 kb on - strand at 196.907 kb on - strand at 196.907 kb on - strand at 196.907 kb on - strand at 196.966 kb on + strand at 196.967 kb on - strand at 196.998 kb on - strand at 197.026 kb on + strand at 197.045 kb on + strand at 197.046 kb on - strand at 197.046 kb on - strand at 197.046 kb on - strand at 197.046 kb on - strand at 197.046 kb on - strand at 197.046 kb on - strand at 197.046 kb on - strand at 197.056 kb on - strand at 197.065 kb on + strand at 197.065 kb on + strand at 197.065 kb on + strand at 197.065 kb on + strand at 197.065 kb on + strand at 197.065 kb on + strand at 197.066 kb on - strand at 197.066 kb on - strand at 197.066 kb on - strand at 197.066 kb on - strand at 197.066 kb on - strand at 197.066 kb on - strand at 197.066 kb on - strand at 197.066 kb on - strand at 197.066 kb on - strand at 197.066 kb on - strand at 197.066 kb on - strand at 197.066 kb on - strand at 197.066 kb on - strand at 197.066 kb on - strand at 197.066 kb on - strand at 197.069 kb on + strand at 197.069 kb on + strand at 197.069 kb on + strand at 197.069 kb on + strand at 197.069 kb on + strand at 197.070 kb on - strand at 197.070 kb on - strand at 197.070 kb on - strand at 197.070 kb on - strand at 197.070 kb on - strand at 197.070 kb on - strand at 197.070 kb on - strand at 197.073 kb on - strand at 197.127 kb on + strand, within EX31_RS18160 at 197.135 kb on + strand, within EX31_RS18160 at 197.136 kb on - strand, within EX31_RS18160 at 197.143 kb on - strand, within EX31_RS18160 at 197.156 kb on + strand, within EX31_RS18160 at 197.211 kb on - strand, within EX31_RS18160 at 197.234 kb on - strand, within EX31_RS18160 at 197.234 kb on - strand, within EX31_RS18160 at 197.234 kb on - strand, within EX31_RS18160 at 197.265 kb on - strand, within EX31_RS18160 at 197.265 kb on - strand, within EX31_RS18160 at 197.267 kb on - strand, within EX31_RS18160 at 197.282 kb on + strand, within EX31_RS18160 at 197.282 kb on + strand, within EX31_RS18160 at 197.283 kb on - strand, within EX31_RS18160 at 197.283 kb on - strand, within EX31_RS18160 at 197.283 kb on - strand, within EX31_RS18160 at 197.283 kb on - strand, within EX31_RS18160 at 197.283 kb on - strand, within EX31_RS18160 at 197.287 kb on - strand, within EX31_RS18160 at 197.332 kb on - strand, within EX31_RS18160 at 197.394 kb on + strand, within EX31_RS18160 at 197.395 kb on - strand, within EX31_RS18160 at 197.395 kb on - strand, within EX31_RS18160 at 197.504 kb on + strand, within EX31_RS18160 at 197.504 kb on + strand, within EX31_RS18160 at 197.504 kb on + strand, within EX31_RS18160 at 197.590 kb on + strand, within EX31_RS18160 at 197.591 kb on - strand, within EX31_RS18160 at 197.593 kb on + strand, within EX31_RS18160 at 197.593 kb on + strand, within EX31_RS18160 at 197.593 kb on + strand, within EX31_RS18160 at 197.593 kb on + strand, within EX31_RS18160 at 197.593 kb on + strand, within EX31_RS18160 at 197.593 kb on + strand, within EX31_RS18160 at 197.593 kb on - strand, within EX31_RS18160 at 197.594 kb on - strand, within EX31_RS18160 at 197.594 kb on - strand, within EX31_RS18160 at 197.594 kb on - strand, within EX31_RS18160 at 197.594 kb on - strand, within EX31_RS18160 at 197.594 kb on - strand, within EX31_RS18160 at 197.606 kb on - strand, within EX31_RS18160 at 197.606 kb on - strand, within EX31_RS18160 at 197.606 kb on - strand, within EX31_RS18160 at 197.687 kb on + strand, within EX31_RS18160 at 197.739 kb on - strand, within EX31_RS18160 at 197.739 kb on - strand, within EX31_RS18160 at 197.742 kb on - strand, within EX31_RS18160 at 197.742 kb on - strand, within EX31_RS18160 at 197.806 kb on + strand, within EX31_RS18160 at 197.807 kb on - strand, within EX31_RS18160 at 197.807 kb on - strand, within EX31_RS18160 at 197.807 kb on - strand, within EX31_RS18160 at 197.883 kb on + strand, within EX31_RS18160 at 197.883 kb on + strand, within EX31_RS18160 at 197.883 kb on + strand, within EX31_RS18160 at 197.884 kb on - strand, within EX31_RS18160 at 197.884 kb on - strand, within EX31_RS18160 at 197.884 kb on - strand, within EX31_RS18160 at 197.994 kb on - strand, within EX31_RS18160 at 198.020 kb on + strand, within EX31_RS18160 at 198.021 kb on - strand, within EX31_RS18160 at 198.188 kb on - strand, within EX31_RS18160 at 198.188 kb on - strand, within EX31_RS18160 at 198.191 kb on - strand, within EX31_RS18160 at 198.220 kb on + strand, within EX31_RS18160 at 198.220 kb on + strand, within EX31_RS18160 at 198.220 kb on + strand, within EX31_RS18160 at 198.230 kb on + strand, within EX31_RS18160 at 198.231 kb on - strand, within EX31_RS18160 at 198.310 kb on + strand, within EX31_RS18160 at 198.310 kb on + strand, within EX31_RS18160 at 198.313 kb on - strand, within EX31_RS18160 at 198.320 kb on + strand, within EX31_RS18160 at 198.321 kb on - strand, within EX31_RS18160 at 198.544 kb on + strand, within EX31_RS18160 at 198.545 kb on - strand, within EX31_RS18160 at 198.545 kb on - strand, within EX31_RS18160 at 198.545 kb on - strand, within EX31_RS18160 at 198.568 kb on + strand, within EX31_RS18160 at 198.568 kb on + strand, within EX31_RS18160 at 198.568 kb on + strand, within EX31_RS18160 at 198.568 kb on + strand, within EX31_RS18160 at 198.568 kb on + strand, within EX31_RS18160 at 198.568 kb on + strand, within EX31_RS18160 at 198.568 kb on + strand, within EX31_RS18160 at 198.569 kb on - strand, within EX31_RS18160 at 198.569 kb on - strand, within EX31_RS18160 at 198.569 kb on - strand, within EX31_RS18160 at 198.569 kb on - strand, within EX31_RS18160 at 198.569 kb on - strand, within EX31_RS18160 at 198.724 kb on - strand at 198.820 kb on + strand at 198.821 kb on - strand at 198.821 kb on - strand at 198.821 kb on - strand at 198.821 kb on - strand at 198.824 kb on + strand at 198.824 kb on + strand at 198.824 kb on + strand at 198.825 kb on - strand at 198.825 kb on - strand at 198.825 kb on - strand at 198.825 kb on - strand at 198.825 kb on - strand at 198.825 kb on - strand at 198.825 kb on - strand at 198.825 kb on - strand at 198.825 kb on - strand at 198.825 kb on - strand at 198.825 kb on - strand at 198.825 kb on - strand at 198.825 kb on - strand at 198.825 kb on - strand at 198.825 kb on - strand at 198.825 kb on - strand at 198.827 kb on - strand at 198.827 kb on - strand at 198.827 kb on - strand at 198.828 kb on + strand at 198.828 kb on + strand at 198.829 kb on - strand at 198.829 kb on - strand at 198.829 kb on - strand at 198.851 kb on - strand at 198.867 kb on - strand at 198.885 kb on - strand at 198.893 kb on + strand at 198.893 kb on + strand at 198.911 kb on - strand at 198.935 kb on + strand at 198.935 kb on + strand at 198.935 kb on + strand at 198.935 kb on + strand at 198.936 kb on - strand at 198.936 kb on - strand at 198.963 kb on - strand at 198.963 kb on - strand at 198.991 kb on - strand at 198.997 kb on + strand at 198.997 kb on + strand at 198.997 kb on + strand at 198.998 kb on - strand at 198.998 kb on - strand at 199.001 kb on + strand at 199.001 kb on + strand at 199.002 kb on - strand at 199.002 kb on - strand at 199.028 kb on - strand at 199.029 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 195,983 - EX31_RS18150 0.78 +0.2 196,060 - -2.6 196,104 + -1.2 196,105 - -2.6 196,107 + +0.2 196,107 + +0.4 196,107 + +0.7 196,108 - -0.4 196,108 - +0.4 196,163 - +0.9 196,163 - +0.2 196,167 - +0.2 196,167 - -1.1 196,167 - +1.1 196,215 + -1.4 196,216 - -0.1 196,229 + -0.6 196,230 - EX31_RS18155 0.10 -0.7 196,234 + EX31_RS18155 0.11 +2.7 196,235 - EX31_RS18155 0.11 +0.7 196,235 - EX31_RS18155 0.11 +0.6 196,235 - EX31_RS18155 0.11 -0.8 196,328 + EX31_RS18155 0.28 -1.4 196,328 + EX31_RS18155 0.28 +1.4 196,328 + EX31_RS18155 0.28 +1.2 196,328 + EX31_RS18155 0.28 -1.0 196,329 - EX31_RS18155 0.28 -0.0 196,329 - EX31_RS18155 0.28 -0.7 196,449 - EX31_RS18155 0.50 -0.2 196,594 - EX31_RS18155 0.76 -0.7 196,699 - -1.1 196,735 - +1.2 196,735 - +1.1 196,735 - -1.8 196,788 + -0.8 196,789 - -0.3 196,789 - -0.7 196,822 + +0.8 196,822 + -0.7 196,822 + -0.5 196,822 + -2.7 196,823 - +0.4 196,823 - +0.5 196,823 - +0.6 196,824 + -1.7 196,824 + -2.2 196,825 - +0.4 196,825 - -0.4 196,825 - +2.6 196,825 - +1.3 196,825 - -0.4 196,872 - +1.2 196,872 - -0.9 196,884 - -0.1 196,891 + -0.8 196,892 - -2.4 196,897 + +0.0 196,897 + +0.4 196,898 - +0.5 196,907 - -0.6 196,907 - +0.1 196,907 - +0.1 196,966 + +0.1 196,967 - +1.4 196,998 - -1.6 197,026 + +1.2 197,045 + -1.1 197,046 - +0.0 197,046 - -0.4 197,046 - +0.4 197,046 - -0.8 197,046 - -1.0 197,046 - +2.0 197,046 - -1.3 197,056 - -0.3 197,065 + -1.1 197,065 + +1.0 197,065 + +2.0 197,065 + +0.3 197,065 + -0.3 197,065 + +0.4 197,066 - +1.1 197,066 - +0.6 197,066 - -0.8 197,066 - +0.9 197,066 - +1.7 197,066 - +0.3 197,066 - +1.1 197,066 - -0.3 197,066 - +0.2 197,066 - -1.2 197,066 - +0.7 197,066 - +0.1 197,066 - -2.1 197,066 - -0.4 197,066 - +1.0 197,069 + -1.4 197,069 + +0.1 197,069 + +0.0 197,069 + +1.3 197,069 + -1.9 197,070 - +2.3 197,070 - +0.2 197,070 - -0.6 197,070 - +0.0 197,070 - -1.7 197,070 - -1.7 197,070 - +0.0 197,073 - -2.3 197,127 + EX31_RS18160 0.12 -0.4 197,135 + EX31_RS18160 0.12 +1.0 197,136 - EX31_RS18160 0.12 +1.0 197,143 - EX31_RS18160 0.13 -0.0 197,156 + EX31_RS18160 0.14 -0.8 197,211 - EX31_RS18160 0.17 -1.7 197,234 - EX31_RS18160 0.18 +0.7 197,234 - EX31_RS18160 0.18 +0.9 197,234 - EX31_RS18160 0.18 -0.6 197,265 - EX31_RS18160 0.19 -1.0 197,265 - EX31_RS18160 0.19 -0.1 197,267 - EX31_RS18160 0.20 +0.9 197,282 + EX31_RS18160 0.20 +1.4 197,282 + EX31_RS18160 0.20 -1.8 197,283 - EX31_RS18160 0.20 +2.3 197,283 - EX31_RS18160 0.20 -0.1 197,283 - EX31_RS18160 0.20 -2.3 197,283 - EX31_RS18160 0.20 -0.1 197,283 - EX31_RS18160 0.20 +0.7 197,287 - EX31_RS18160 0.21 +0.6 197,332 - EX31_RS18160 0.23 +0.5 197,394 + EX31_RS18160 0.26 +0.4 197,395 - EX31_RS18160 0.26 -1.7 197,395 - EX31_RS18160 0.26 -0.8 197,504 + EX31_RS18160 0.32 -1.1 197,504 + EX31_RS18160 0.32 -0.7 197,504 + EX31_RS18160 0.32 +0.0 197,590 + EX31_RS18160 0.37 -0.2 197,591 - EX31_RS18160 0.37 +0.4 197,593 + EX31_RS18160 0.37 +0.0 197,593 + EX31_RS18160 0.37 +0.4 197,593 + EX31_RS18160 0.37 -0.1 197,593 + EX31_RS18160 0.37 -1.0 197,593 + EX31_RS18160 0.37 +3.0 197,593 + EX31_RS18160 0.37 +2.0 197,593 - EX31_RS18160 0.37 +0.0 197,594 - EX31_RS18160 0.37 +0.5 197,594 - EX31_RS18160 0.37 +0.1 197,594 - EX31_RS18160 0.37 -0.2 197,594 - EX31_RS18160 0.37 +0.4 197,594 - EX31_RS18160 0.37 +0.6 197,606 - EX31_RS18160 0.38 +2.8 197,606 - EX31_RS18160 0.38 -0.2 197,606 - EX31_RS18160 0.38 +0.8 197,687 + EX31_RS18160 0.42 -0.3 197,739 - EX31_RS18160 0.45 -0.6 197,739 - EX31_RS18160 0.45 -1.8 197,742 - EX31_RS18160 0.45 +0.8 197,742 - EX31_RS18160 0.45 -0.6 197,806 + EX31_RS18160 0.49 +1.0 197,807 - EX31_RS18160 0.49 -0.3 197,807 - EX31_RS18160 0.49 +0.3 197,807 - EX31_RS18160 0.49 +0.2 197,883 + EX31_RS18160 0.53 +0.6 197,883 + EX31_RS18160 0.53 +1.0 197,883 + EX31_RS18160 0.53 +0.2 197,884 - EX31_RS18160 0.53 +0.4 197,884 - EX31_RS18160 0.53 +0.1 197,884 - EX31_RS18160 0.53 +1.0 197,994 - EX31_RS18160 0.59 -1.0 198,020 + EX31_RS18160 0.60 -0.7 198,021 - EX31_RS18160 0.60 +1.5 198,188 - EX31_RS18160 0.69 -2.2 198,188 - EX31_RS18160 0.69 +0.8 198,191 - EX31_RS18160 0.69 +0.4 198,220 + EX31_RS18160 0.71 -0.7 198,220 + EX31_RS18160 0.71 +0.6 198,220 + EX31_RS18160 0.71 -1.1 198,230 + EX31_RS18160 0.72 +1.0 198,231 - EX31_RS18160 0.72 -1.2 198,310 + EX31_RS18160 0.76 +1.0 198,310 + EX31_RS18160 0.76 +0.3 198,313 - EX31_RS18160 0.76 -1.3 198,320 + EX31_RS18160 0.76 +1.2 198,321 - EX31_RS18160 0.76 -0.4 198,544 + EX31_RS18160 0.89 -0.8 198,545 - EX31_RS18160 0.89 +1.2 198,545 - EX31_RS18160 0.89 -0.1 198,545 - EX31_RS18160 0.89 +0.9 198,568 + EX31_RS18160 0.90 -0.8 198,568 + EX31_RS18160 0.90 -2.3 198,568 + EX31_RS18160 0.90 +0.3 198,568 + EX31_RS18160 0.90 -1.3 198,568 + EX31_RS18160 0.90 -1.4 198,568 + EX31_RS18160 0.90 +0.8 198,568 + EX31_RS18160 0.90 -0.6 198,569 - EX31_RS18160 0.90 +1.4 198,569 - EX31_RS18160 0.90 -0.0 198,569 - EX31_RS18160 0.90 +0.0 198,569 - EX31_RS18160 0.90 +1.0 198,569 - EX31_RS18160 0.90 -0.1 198,724 - -0.7 198,820 + -0.7 198,821 - +1.3 198,821 - +0.7 198,821 - +0.8 198,821 - +0.9 198,824 + -2.6 198,824 + +0.3 198,824 + -1.2 198,825 - -0.2 198,825 - +1.2 198,825 - -0.1 198,825 - +0.3 198,825 - -0.4 198,825 - -0.9 198,825 - +0.0 198,825 - +0.0 198,825 - -0.1 198,825 - +2.6 198,825 - -1.1 198,825 - +0.4 198,825 - +1.2 198,825 - +0.8 198,825 - +2.0 198,825 - +2.0 198,827 - +1.8 198,827 - +1.2 198,827 - +1.0 198,828 + -0.1 198,828 + +0.3 198,829 - +0.6 198,829 - +0.5 198,829 - +0.6 198,851 - +1.0 198,867 - +1.0 198,885 - -1.7 198,893 + -0.3 198,893 + -1.0 198,911 - +2.0 198,935 + +0.8 198,935 + +0.7 198,935 + -0.6 198,935 + +0.6 198,936 - -0.3 198,936 - +0.6 198,963 - +0.9 198,963 - +0.4 198,991 - +0.3 198,997 + -2.4 198,997 + -0.9 198,997 + +0.0 198,998 - +0.0 198,998 - -0.1 199,001 + +1.6 199,001 + -1.1 199,002 - -0.1 199,002 - -1.0 199,028 - -0.2 199,029 + -0.8
Or see this region's nucleotide sequence