Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS17590 and EX31_RS17595 are separated by 337 nucleotides EX31_RS17595 and EX31_RS17600 are separated by 4 nucleotides EX31_RS17600 and EX31_RS17605 are separated by 228 nucleotides
EX31_RS17590: EX31_RS17590 - thymidylate kinase, at 71,662 to 72,279
_RS17590
EX31_RS17595: EX31_RS17595 - chorismate mutase, at 72,617 to 72,913
_RS17595
EX31_RS17600: EX31_RS17600 - LysE family translocator, at 72,918 to 73,457
_RS17600
EX31_RS17605: EX31_RS17605 - pyridoxamine 5'-phosphate oxidase family protein, at 73,686 to 74,309
_RS17605
Position (kb) 72
73 Strain fitness (log2 ratio) -2
-1
0
1
2 at 71.664 kb on - strand at 71.664 kb on - strand at 71.664 kb on - strand at 71.684 kb on + strand at 71.697 kb on + strand at 71.697 kb on + strand at 71.698 kb on - strand at 71.734 kb on - strand, within EX31_RS17590 at 71.741 kb on - strand, within EX31_RS17590 at 71.808 kb on + strand, within EX31_RS17590 at 71.809 kb on - strand, within EX31_RS17590 at 71.809 kb on - strand, within EX31_RS17590 at 71.809 kb on - strand, within EX31_RS17590 at 71.820 kb on + strand, within EX31_RS17590 at 71.821 kb on - strand, within EX31_RS17590 at 71.821 kb on - strand, within EX31_RS17590 at 71.823 kb on + strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.824 kb on - strand, within EX31_RS17590 at 71.827 kb on + strand, within EX31_RS17590 at 71.828 kb on - strand, within EX31_RS17590 at 71.866 kb on - strand, within EX31_RS17590 at 71.866 kb on - strand, within EX31_RS17590 at 71.926 kb on - strand, within EX31_RS17590 at 71.926 kb on - strand, within EX31_RS17590 at 71.926 kb on - strand, within EX31_RS17590 at 72.000 kb on + strand, within EX31_RS17590 at 72.000 kb on + strand, within EX31_RS17590 at 72.000 kb on + strand, within EX31_RS17590 at 72.001 kb on - strand, within EX31_RS17590 at 72.001 kb on - strand, within EX31_RS17590 at 72.001 kb on - strand, within EX31_RS17590 at 72.001 kb on - strand, within EX31_RS17590 at 72.001 kb on - strand, within EX31_RS17590 at 72.144 kb on - strand, within EX31_RS17590 at 72.144 kb on - strand, within EX31_RS17590 at 72.261 kb on + strand at 72.262 kb on - strand at 72.262 kb on - strand at 72.299 kb on - strand at 72.299 kb on - strand at 72.299 kb on - strand at 72.338 kb on + strand at 72.338 kb on + strand at 72.339 kb on - strand at 72.339 kb on - strand at 72.339 kb on - strand at 72.414 kb on + strand at 72.414 kb on + strand at 72.414 kb on + strand at 72.414 kb on + strand at 72.433 kb on - strand at 72.503 kb on + strand at 72.504 kb on - strand at 72.510 kb on + strand at 72.511 kb on - strand at 72.515 kb on + strand at 72.515 kb on + strand at 72.515 kb on + strand at 72.515 kb on + strand at 72.516 kb on - strand at 72.516 kb on - strand at 72.516 kb on - strand at 72.516 kb on - strand at 72.517 kb on + strand at 72.517 kb on + strand at 72.517 kb on + strand at 72.517 kb on + strand at 72.517 kb on + strand at 72.517 kb on + strand at 72.517 kb on + strand at 72.517 kb on + strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.518 kb on - strand at 72.519 kb on + strand at 72.519 kb on + strand at 72.519 kb on + strand at 72.519 kb on + strand at 72.519 kb on + strand at 72.520 kb on - strand at 72.520 kb on - strand at 72.520 kb on - strand at 72.520 kb on - strand at 72.520 kb on - strand at 72.520 kb on - strand at 72.520 kb on - strand at 72.520 kb on - strand at 72.558 kb on + strand at 72.559 kb on - strand at 72.578 kb on + strand at 72.578 kb on + strand at 72.579 kb on - strand at 72.579 kb on - strand at 72.579 kb on - strand at 72.644 kb on - strand at 72.679 kb on + strand, within EX31_RS17595 at 72.709 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.786 kb on + strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.787 kb on - strand, within EX31_RS17595 at 72.929 kb on + strand at 72.929 kb on + strand at 72.929 kb on + strand at 72.930 kb on - strand at 72.942 kb on - strand at 73.064 kb on + strand, within EX31_RS17600 at 73.077 kb on + strand, within EX31_RS17600 at 73.078 kb on - strand, within EX31_RS17600 at 73.078 kb on - strand, within EX31_RS17600 at 73.078 kb on - strand, within EX31_RS17600 at 73.078 kb on - strand, within EX31_RS17600 at 73.150 kb on + strand, within EX31_RS17600 at 73.196 kb on + strand, within EX31_RS17600 at 73.196 kb on + strand, within EX31_RS17600 at 73.258 kb on + strand, within EX31_RS17600 at 73.258 kb on + strand, within EX31_RS17600 at 73.258 kb on + strand, within EX31_RS17600 at 73.259 kb on - strand, within EX31_RS17600 at 73.259 kb on - strand, within EX31_RS17600 at 73.259 kb on - strand, within EX31_RS17600 at 73.259 kb on - strand, within EX31_RS17600 at 73.329 kb on + strand, within EX31_RS17600 at 73.330 kb on - strand, within EX31_RS17600 at 73.352 kb on + strand, within EX31_RS17600 at 73.352 kb on + strand, within EX31_RS17600 at 73.353 kb on - strand, within EX31_RS17600 at 73.358 kb on + strand, within EX31_RS17600 at 73.358 kb on + strand, within EX31_RS17600 at 73.358 kb on + strand, within EX31_RS17600 at 73.359 kb on - strand, within EX31_RS17600 at 73.359 kb on - strand, within EX31_RS17600 at 73.363 kb on + strand, within EX31_RS17600 at 73.363 kb on + strand, within EX31_RS17600 at 73.363 kb on + strand, within EX31_RS17600 at 73.363 kb on + strand, within EX31_RS17600 at 73.456 kb on - strand at 73.456 kb on - strand at 73.471 kb on + strand at 73.471 kb on + strand at 73.471 kb on + strand at 73.472 kb on - strand at 73.472 kb on - strand at 73.472 kb on - strand at 73.475 kb on + strand at 73.475 kb on + strand at 73.475 kb on + strand at 73.476 kb on - strand at 73.496 kb on + strand at 73.496 kb on + strand at 73.496 kb on + strand at 73.497 kb on - strand at 73.497 kb on - strand at 73.497 kb on - strand at 73.498 kb on + strand at 73.498 kb on + strand at 73.499 kb on - strand at 73.499 kb on - strand at 73.505 kb on + strand at 73.505 kb on + strand at 73.505 kb on + strand at 73.505 kb on + strand at 73.506 kb on - strand at 73.540 kb on + strand at 73.579 kb on - strand at 73.595 kb on + strand at 73.596 kb on - strand at 73.596 kb on - strand at 73.599 kb on + strand at 73.600 kb on - strand at 73.600 kb on - strand at 73.600 kb on - strand at 73.600 kb on - strand at 73.639 kb on + strand at 73.639 kb on + strand at 73.643 kb on + strand at 73.643 kb on + strand at 73.709 kb on + strand at 73.709 kb on + strand at 73.709 kb on + strand at 73.718 kb on + strand at 73.805 kb on + strand, within EX31_RS17605 at 73.893 kb on + strand, within EX31_RS17605 at 73.893 kb on + strand, within EX31_RS17605 at 73.893 kb on + strand, within EX31_RS17605 at 73.893 kb on + strand, within EX31_RS17605
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 71,664 - +1.6 71,664 - +1.8 71,664 - +1.0 71,684 + -0.2 71,697 + +0.1 71,697 + +1.4 71,698 - +0.8 71,734 - EX31_RS17590 0.12 -0.1 71,741 - EX31_RS17590 0.13 -1.3 71,808 + EX31_RS17590 0.24 +0.2 71,809 - EX31_RS17590 0.24 +0.1 71,809 - EX31_RS17590 0.24 -0.3 71,809 - EX31_RS17590 0.24 +0.5 71,820 + EX31_RS17590 0.26 +0.0 71,821 - EX31_RS17590 0.26 +1.2 71,821 - EX31_RS17590 0.26 -0.1 71,823 + EX31_RS17590 0.26 +2.0 71,824 - EX31_RS17590 0.26 +0.4 71,824 - EX31_RS17590 0.26 -0.3 71,824 - EX31_RS17590 0.26 -1.9 71,824 - EX31_RS17590 0.26 +0.7 71,824 - EX31_RS17590 0.26 +0.2 71,824 - EX31_RS17590 0.26 +0.9 71,824 - EX31_RS17590 0.26 +0.8 71,824 - EX31_RS17590 0.26 -1.7 71,824 - EX31_RS17590 0.26 -1.0 71,827 + EX31_RS17590 0.27 +1.1 71,828 - EX31_RS17590 0.27 +0.2 71,866 - EX31_RS17590 0.33 -0.1 71,866 - EX31_RS17590 0.33 +0.3 71,926 - EX31_RS17590 0.43 +0.1 71,926 - EX31_RS17590 0.43 -0.5 71,926 - EX31_RS17590 0.43 -0.5 72,000 + EX31_RS17590 0.55 -2.3 72,000 + EX31_RS17590 0.55 -0.4 72,000 + EX31_RS17590 0.55 -0.3 72,001 - EX31_RS17590 0.55 -0.3 72,001 - EX31_RS17590 0.55 +1.6 72,001 - EX31_RS17590 0.55 +0.6 72,001 - EX31_RS17590 0.55 +1.4 72,001 - EX31_RS17590 0.55 +2.5 72,144 - EX31_RS17590 0.78 +0.7 72,144 - EX31_RS17590 0.78 +2.5 72,261 + +0.7 72,262 - -1.3 72,262 - +0.3 72,299 - +0.6 72,299 - +1.2 72,299 - +1.0 72,338 + +0.4 72,338 + -0.6 72,339 - +0.4 72,339 - -1.2 72,339 - +0.7 72,414 + -2.6 72,414 + +0.6 72,414 + +0.7 72,414 + +0.0 72,433 - +0.3 72,503 + +0.4 72,504 - +0.6 72,510 + -0.9 72,511 - +0.7 72,515 + -0.5 72,515 + +0.9 72,515 + -1.7 72,515 + -2.1 72,516 - +0.6 72,516 - -0.9 72,516 - +0.0 72,516 - -0.4 72,517 + +0.3 72,517 + +0.9 72,517 + +0.3 72,517 + -0.3 72,517 + -1.1 72,517 + +0.4 72,517 + +2.6 72,517 + -0.8 72,518 - -0.2 72,518 - -0.9 72,518 - +0.0 72,518 - -1.0 72,518 - +0.3 72,518 - -2.7 72,518 - +1.6 72,518 - +0.8 72,518 - -0.1 72,519 + -0.1 72,519 + -1.1 72,519 + +0.2 72,519 + +1.4 72,519 + +0.3 72,520 - +1.3 72,520 - +0.1 72,520 - +1.9 72,520 - +1.4 72,520 - +0.9 72,520 - -0.6 72,520 - -0.3 72,520 - +0.0 72,558 + +0.8 72,559 - -0.6 72,578 + -0.4 72,578 + -0.6 72,579 - -0.3 72,579 - +0.6 72,579 - +0.7 72,644 - -0.8 72,679 + EX31_RS17595 0.21 -0.9 72,709 + EX31_RS17595 0.31 +0.2 72,786 + EX31_RS17595 0.57 -1.8 72,786 + EX31_RS17595 0.57 -0.0 72,786 + EX31_RS17595 0.57 +0.0 72,786 + EX31_RS17595 0.57 +0.2 72,786 + EX31_RS17595 0.57 -0.7 72,786 + EX31_RS17595 0.57 +0.4 72,787 - EX31_RS17595 0.57 +0.0 72,787 - EX31_RS17595 0.57 +2.0 72,787 - EX31_RS17595 0.57 -0.8 72,787 - EX31_RS17595 0.57 -0.6 72,787 - EX31_RS17595 0.57 -0.3 72,787 - EX31_RS17595 0.57 -1.7 72,787 - EX31_RS17595 0.57 +1.5 72,929 + +0.8 72,929 + -0.1 72,929 + -0.9 72,930 - +1.8 72,942 - -0.6 73,064 + EX31_RS17600 0.27 +0.6 73,077 + EX31_RS17600 0.29 -0.6 73,078 - EX31_RS17600 0.30 +1.3 73,078 - EX31_RS17600 0.30 +0.4 73,078 - EX31_RS17600 0.30 +1.6 73,078 - EX31_RS17600 0.30 -0.4 73,150 + EX31_RS17600 0.43 -1.4 73,196 + EX31_RS17600 0.51 +0.6 73,196 + EX31_RS17600 0.51 -1.3 73,258 + EX31_RS17600 0.63 +0.1 73,258 + EX31_RS17600 0.63 -0.1 73,258 + EX31_RS17600 0.63 -0.8 73,259 - EX31_RS17600 0.63 +2.4 73,259 - EX31_RS17600 0.63 -1.4 73,259 - EX31_RS17600 0.63 +2.0 73,259 - EX31_RS17600 0.63 +1.0 73,329 + EX31_RS17600 0.76 +0.4 73,330 - EX31_RS17600 0.76 -0.6 73,352 + EX31_RS17600 0.80 -0.3 73,352 + EX31_RS17600 0.80 +1.0 73,353 - EX31_RS17600 0.81 -0.7 73,358 + EX31_RS17600 0.81 +0.2 73,358 + EX31_RS17600 0.81 +2.6 73,358 + EX31_RS17600 0.81 +0.0 73,359 - EX31_RS17600 0.82 -0.8 73,359 - EX31_RS17600 0.82 +0.2 73,363 + EX31_RS17600 0.82 +1.8 73,363 + EX31_RS17600 0.82 -0.0 73,363 + EX31_RS17600 0.82 +0.1 73,363 + EX31_RS17600 0.82 +0.5 73,456 - +1.4 73,456 - -0.6 73,471 + -1.2 73,471 + -0.8 73,471 + +0.8 73,472 - +0.1 73,472 - +0.3 73,472 - +0.5 73,475 + +0.2 73,475 + +0.0 73,475 + +0.5 73,476 - +0.5 73,496 + -0.6 73,496 + +0.5 73,496 + -0.6 73,497 - +0.0 73,497 - +0.0 73,497 - -0.9 73,498 + -2.4 73,498 + +2.0 73,499 - +0.0 73,499 - +0.4 73,505 + -0.6 73,505 + +0.8 73,505 + -0.9 73,505 + +0.7 73,506 - +1.0 73,540 + +0.1 73,579 - -1.0 73,595 + -1.0 73,596 - +0.6 73,596 - +1.4 73,599 + +0.4 73,600 - -0.2 73,600 - +0.0 73,600 - +1.0 73,600 - -0.2 73,639 + +0.9 73,639 + -0.8 73,643 + +1.0 73,643 + -0.9 73,709 + +1.3 73,709 + -0.7 73,709 + -1.7 73,718 + +0.4 73,805 + EX31_RS17605 0.19 +0.6 73,893 + EX31_RS17605 0.33 +1.2 73,893 + EX31_RS17605 0.33 -1.3 73,893 + EX31_RS17605 0.33 +1.4 73,893 + EX31_RS17605 0.33 -0.3
Or see this region's nucleotide sequence