Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS17345 and EX31_RS17350 are separated by 177 nucleotides EX31_RS17350 and EX31_RS17355 are separated by 150 nucleotides EX31_RS17355 and EX31_RS17360 are separated by 73 nucleotides
EX31_RS17345: EX31_RS17345 - multidrug efflux MFS transporter MdtH, at 18,558 to 19,763
_RS17345
EX31_RS17350: EX31_RS17350 - glutaredoxin 2, at 19,941 to 20,588
_RS17350
EX31_RS17355: EX31_RS17355 - lipoprotein, at 20,739 to 21,302
_RS17355
EX31_RS17360: EX31_RS17360 - molecular chaperone, at 21,376 to 21,960
_RS17360
Position (kb) 19
20
21 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 19.038 kb on + strand, within EX31_RS17345 at 19.038 kb on + strand, within EX31_RS17345 at 19.039 kb on - strand, within EX31_RS17345 at 19.051 kb on - strand, within EX31_RS17345 at 19.051 kb on - strand, within EX31_RS17345 at 19.119 kb on + strand, within EX31_RS17345 at 19.194 kb on + strand, within EX31_RS17345 at 19.194 kb on + strand, within EX31_RS17345 at 19.195 kb on - strand, within EX31_RS17345 at 19.198 kb on + strand, within EX31_RS17345 at 19.198 kb on + strand, within EX31_RS17345 at 19.198 kb on + strand, within EX31_RS17345 at 19.199 kb on - strand, within EX31_RS17345 at 19.215 kb on + strand, within EX31_RS17345 at 19.215 kb on + strand, within EX31_RS17345 at 19.215 kb on + strand, within EX31_RS17345 at 19.218 kb on + strand, within EX31_RS17345 at 19.218 kb on + strand, within EX31_RS17345 at 19.218 kb on + strand, within EX31_RS17345 at 19.218 kb on + strand, within EX31_RS17345 at 19.218 kb on + strand, within EX31_RS17345 at 19.218 kb on + strand, within EX31_RS17345 at 19.218 kb on + strand, within EX31_RS17345 at 19.218 kb on + strand, within EX31_RS17345 at 19.218 kb on + strand, within EX31_RS17345 at 19.218 kb on + strand, within EX31_RS17345 at 19.218 kb on + strand, within EX31_RS17345 at 19.218 kb on + strand, within EX31_RS17345 at 19.218 kb on + strand, within EX31_RS17345 at 19.218 kb on + strand, within EX31_RS17345 at 19.218 kb on + strand, within EX31_RS17345 at 19.219 kb on - strand, within EX31_RS17345 at 19.219 kb on - strand, within EX31_RS17345 at 19.219 kb on - strand, within EX31_RS17345 at 19.219 kb on - strand, within EX31_RS17345 at 19.219 kb on - strand, within EX31_RS17345 at 19.219 kb on - strand, within EX31_RS17345 at 19.219 kb on - strand, within EX31_RS17345 at 19.219 kb on - strand, within EX31_RS17345 at 19.302 kb on + strand, within EX31_RS17345 at 19.302 kb on + strand, within EX31_RS17345 at 19.302 kb on + strand, within EX31_RS17345 at 19.303 kb on - strand, within EX31_RS17345 at 19.303 kb on - strand, within EX31_RS17345 at 19.303 kb on - strand, within EX31_RS17345 at 19.339 kb on - strand, within EX31_RS17345 at 19.339 kb on - strand, within EX31_RS17345 at 19.439 kb on + strand, within EX31_RS17345 at 19.479 kb on + strand, within EX31_RS17345 at 19.557 kb on + strand, within EX31_RS17345 at 19.557 kb on + strand, within EX31_RS17345 at 19.557 kb on + strand, within EX31_RS17345 at 19.557 kb on + strand, within EX31_RS17345 at 19.557 kb on + strand, within EX31_RS17345 at 19.558 kb on - strand, within EX31_RS17345 at 19.558 kb on - strand, within EX31_RS17345 at 19.558 kb on - strand, within EX31_RS17345 at 19.558 kb on - strand, within EX31_RS17345 at 19.558 kb on - strand, within EX31_RS17345 at 19.558 kb on - strand, within EX31_RS17345 at 19.558 kb on - strand, within EX31_RS17345 at 19.607 kb on + strand, within EX31_RS17345 at 19.626 kb on + strand, within EX31_RS17345 at 19.626 kb on + strand, within EX31_RS17345 at 19.627 kb on - strand, within EX31_RS17345 at 19.627 kb on - strand, within EX31_RS17345 at 19.627 kb on - strand, within EX31_RS17345 at 19.669 kb on + strand at 19.670 kb on - strand at 19.673 kb on - strand at 19.711 kb on - strand at 19.779 kb on + strand at 19.838 kb on + strand at 19.838 kb on + strand at 19.838 kb on + strand at 19.838 kb on + strand at 19.838 kb on + strand at 19.838 kb on + strand at 19.839 kb on - strand at 19.839 kb on - strand at 19.839 kb on - strand at 19.839 kb on - strand at 19.839 kb on - strand at 19.839 kb on - strand at 19.839 kb on - strand at 19.839 kb on - strand at 19.841 kb on - strand at 19.847 kb on - strand at 19.863 kb on - strand at 19.880 kb on - strand at 19.880 kb on - strand at 19.880 kb on - strand at 19.904 kb on + strand at 19.904 kb on + strand at 19.904 kb on + strand at 19.933 kb on + strand at 19.933 kb on + strand at 19.933 kb on + strand at 19.934 kb on - strand at 19.934 kb on - strand at 19.934 kb on - strand at 19.934 kb on - strand at 19.934 kb on - strand at 19.937 kb on + strand at 19.937 kb on + strand at 19.938 kb on - strand at 19.938 kb on - strand at 19.938 kb on - strand at 19.938 kb on - strand at 19.938 kb on - strand at 19.938 kb on - strand at 19.941 kb on - strand at 19.941 kb on - strand at 19.941 kb on - strand at 19.941 kb on - strand at 19.941 kb on - strand at 19.950 kb on + strand at 19.951 kb on - strand at 20.126 kb on + strand, within EX31_RS17350 at 20.142 kb on + strand, within EX31_RS17350 at 20.142 kb on + strand, within EX31_RS17350 at 20.142 kb on + strand, within EX31_RS17350 at 20.142 kb on + strand, within EX31_RS17350 at 20.142 kb on + strand, within EX31_RS17350 at 20.143 kb on - strand, within EX31_RS17350 at 20.143 kb on - strand, within EX31_RS17350 at 20.143 kb on - strand, within EX31_RS17350 at 20.143 kb on - strand, within EX31_RS17350 at 20.210 kb on - strand, within EX31_RS17350 at 20.210 kb on - strand, within EX31_RS17350 at 20.227 kb on - strand, within EX31_RS17350 at 20.228 kb on + strand, within EX31_RS17350 at 20.228 kb on + strand, within EX31_RS17350 at 20.301 kb on + strand, within EX31_RS17350 at 20.459 kb on + strand, within EX31_RS17350 at 20.459 kb on + strand, within EX31_RS17350 at 20.460 kb on - strand, within EX31_RS17350 at 20.460 kb on - strand, within EX31_RS17350 at 20.538 kb on - strand at 20.541 kb on - strand at 20.560 kb on + strand at 20.560 kb on + strand at 20.587 kb on - strand at 20.597 kb on + strand at 20.598 kb on - strand at 20.637 kb on + strand at 20.638 kb on - strand at 20.657 kb on + strand at 20.657 kb on + strand at 20.657 kb on + strand at 20.657 kb on + strand at 20.657 kb on + strand at 20.657 kb on + strand at 20.658 kb on - strand at 20.658 kb on - strand at 20.658 kb on - strand at 20.697 kb on - strand at 20.716 kb on - strand at 20.723 kb on - strand at 20.723 kb on - strand at 20.726 kb on + strand at 20.726 kb on + strand at 20.726 kb on + strand at 20.726 kb on + strand at 20.726 kb on + strand at 20.767 kb on + strand at 20.786 kb on - strand at 20.786 kb on - strand at 20.814 kb on + strand, within EX31_RS17355 at 20.815 kb on - strand, within EX31_RS17355 at 20.989 kb on + strand, within EX31_RS17355 at 20.989 kb on + strand, within EX31_RS17355 at 20.989 kb on + strand, within EX31_RS17355 at 20.989 kb on + strand, within EX31_RS17355 at 20.989 kb on + strand, within EX31_RS17355 at 20.989 kb on + strand, within EX31_RS17355 at 21.051 kb on + strand, within EX31_RS17355 at 21.051 kb on + strand, within EX31_RS17355 at 21.102 kb on + strand, within EX31_RS17355 at 21.103 kb on - strand, within EX31_RS17355 at 21.103 kb on - strand, within EX31_RS17355 at 21.153 kb on + strand, within EX31_RS17355 at 21.153 kb on + strand, within EX31_RS17355 at 21.177 kb on + strand, within EX31_RS17355 at 21.177 kb on + strand, within EX31_RS17355 at 21.177 kb on + strand, within EX31_RS17355 at 21.177 kb on + strand, within EX31_RS17355 at 21.178 kb on - strand, within EX31_RS17355 at 21.178 kb on - strand, within EX31_RS17355 at 21.198 kb on + strand, within EX31_RS17355 at 21.198 kb on + strand, within EX31_RS17355 at 21.198 kb on + strand, within EX31_RS17355 at 21.198 kb on + strand, within EX31_RS17355 at 21.198 kb on + strand, within EX31_RS17355 at 21.199 kb on - strand, within EX31_RS17355 at 21.286 kb on + strand at 21.300 kb on + strand at 21.320 kb on + strand at 21.359 kb on - strand at 21.378 kb on - strand at 21.546 kb on - strand, within EX31_RS17360 at 21.546 kb on - strand, within EX31_RS17360 at 21.561 kb on + strand, within EX31_RS17360 at 21.562 kb on - strand, within EX31_RS17360 at 21.562 kb on - strand, within EX31_RS17360 at 21.562 kb on - strand, within EX31_RS17360 at 21.562 kb on - strand, within EX31_RS17360
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 19,038 + EX31_RS17345 0.40 +0.3 19,038 + EX31_RS17345 0.40 -0.3 19,039 - EX31_RS17345 0.40 +2.0 19,051 - EX31_RS17345 0.41 +0.4 19,051 - EX31_RS17345 0.41 +1.8 19,119 + EX31_RS17345 0.47 -1.6 19,194 + EX31_RS17345 0.53 +0.6 19,194 + EX31_RS17345 0.53 +0.0 19,195 - EX31_RS17345 0.53 -0.3 19,198 + EX31_RS17345 0.53 -1.3 19,198 + EX31_RS17345 0.53 -1.2 19,198 + EX31_RS17345 0.53 -1.6 19,199 - EX31_RS17345 0.53 +0.4 19,215 + EX31_RS17345 0.54 +0.3 19,215 + EX31_RS17345 0.54 +1.1 19,215 + EX31_RS17345 0.54 +1.0 19,218 + EX31_RS17345 0.55 -1.4 19,218 + EX31_RS17345 0.55 +0.9 19,218 + EX31_RS17345 0.55 +0.7 19,218 + EX31_RS17345 0.55 +0.3 19,218 + EX31_RS17345 0.55 +0.7 19,218 + EX31_RS17345 0.55 +1.6 19,218 + EX31_RS17345 0.55 +0.1 19,218 + EX31_RS17345 0.55 +1.6 19,218 + EX31_RS17345 0.55 -0.6 19,218 + EX31_RS17345 0.55 -0.6 19,218 + EX31_RS17345 0.55 +1.0 19,218 + EX31_RS17345 0.55 -2.1 19,218 + EX31_RS17345 0.55 -0.4 19,218 + EX31_RS17345 0.55 -0.7 19,218 + EX31_RS17345 0.55 +1.3 19,219 - EX31_RS17345 0.55 +0.2 19,219 - EX31_RS17345 0.55 +0.8 19,219 - EX31_RS17345 0.55 +1.7 19,219 - EX31_RS17345 0.55 -1.6 19,219 - EX31_RS17345 0.55 +0.4 19,219 - EX31_RS17345 0.55 +0.7 19,219 - EX31_RS17345 0.55 -0.3 19,219 - EX31_RS17345 0.55 +1.6 19,302 + EX31_RS17345 0.62 -0.1 19,302 + EX31_RS17345 0.62 +0.1 19,302 + EX31_RS17345 0.62 +0.7 19,303 - EX31_RS17345 0.62 +1.6 19,303 - EX31_RS17345 0.62 -1.7 19,303 - EX31_RS17345 0.62 -0.6 19,339 - EX31_RS17345 0.65 +1.2 19,339 - EX31_RS17345 0.65 -0.1 19,439 + EX31_RS17345 0.73 -0.2 19,479 + EX31_RS17345 0.76 +0.3 19,557 + EX31_RS17345 0.83 -0.3 19,557 + EX31_RS17345 0.83 -0.6 19,557 + EX31_RS17345 0.83 +3.6 19,557 + EX31_RS17345 0.83 -0.6 19,557 + EX31_RS17345 0.83 -0.1 19,558 - EX31_RS17345 0.83 +0.6 19,558 - EX31_RS17345 0.83 +1.6 19,558 - EX31_RS17345 0.83 -0.4 19,558 - EX31_RS17345 0.83 -0.5 19,558 - EX31_RS17345 0.83 +0.8 19,558 - EX31_RS17345 0.83 -0.1 19,558 - EX31_RS17345 0.83 -2.2 19,607 + EX31_RS17345 0.87 -0.4 19,626 + EX31_RS17345 0.89 +0.2 19,626 + EX31_RS17345 0.89 -0.6 19,627 - EX31_RS17345 0.89 -0.6 19,627 - EX31_RS17345 0.89 -0.3 19,627 - EX31_RS17345 0.89 -0.6 19,669 + +0.0 19,670 - +1.4 19,673 - +0.7 19,711 - +0.4 19,779 + -1.4 19,838 + -2.0 19,838 + +0.2 19,838 + -1.2 19,838 + -1.0 19,838 + +0.1 19,838 + -1.4 19,839 - -0.8 19,839 - +1.5 19,839 - +1.7 19,839 - +0.8 19,839 - -0.2 19,839 - -1.5 19,839 - -0.8 19,839 - -0.3 19,841 - +0.1 19,847 - +1.6 19,863 - -0.5 19,880 - -1.2 19,880 - +0.2 19,880 - -0.4 19,904 + +0.9 19,904 + +2.3 19,904 + -0.8 19,933 + +0.6 19,933 + +0.4 19,933 + -0.1 19,934 - +1.7 19,934 - +0.8 19,934 - +1.8 19,934 - -1.1 19,934 - +1.3 19,937 + -0.1 19,937 + -3.0 19,938 - -1.3 19,938 - +0.4 19,938 - +0.6 19,938 - +0.1 19,938 - -2.3 19,938 - -1.3 19,941 - -0.3 19,941 - +0.1 19,941 - -1.8 19,941 - -1.6 19,941 - +0.2 19,950 + +2.7 19,951 - -0.4 20,126 + EX31_RS17350 0.29 -0.2 20,142 + EX31_RS17350 0.31 +1.0 20,142 + EX31_RS17350 0.31 -0.3 20,142 + EX31_RS17350 0.31 -0.6 20,142 + EX31_RS17350 0.31 +1.3 20,142 + EX31_RS17350 0.31 -3.2 20,143 - EX31_RS17350 0.31 +0.8 20,143 - EX31_RS17350 0.31 -0.6 20,143 - EX31_RS17350 0.31 -0.6 20,143 - EX31_RS17350 0.31 -1.4 20,210 - EX31_RS17350 0.42 -1.2 20,210 - EX31_RS17350 0.42 -0.2 20,227 - EX31_RS17350 0.44 -0.2 20,228 + EX31_RS17350 0.44 +2.0 20,228 + EX31_RS17350 0.44 -0.9 20,301 + EX31_RS17350 0.56 -0.4 20,459 + EX31_RS17350 0.80 +0.3 20,459 + EX31_RS17350 0.80 +0.4 20,460 - EX31_RS17350 0.80 -0.6 20,460 - EX31_RS17350 0.80 +0.3 20,538 - +0.7 20,541 - +0.7 20,560 + +2.4 20,560 + -0.6 20,587 - -0.5 20,597 + -1.3 20,598 - +2.0 20,637 + +1.7 20,638 - +0.0 20,657 + +0.8 20,657 + -1.2 20,657 + -0.8 20,657 + +1.6 20,657 + +0.8 20,657 + -0.3 20,658 - -0.5 20,658 - +0.8 20,658 - -0.3 20,697 - +1.7 20,716 - +2.0 20,723 - -0.3 20,723 - +1.8 20,726 + -0.8 20,726 + +1.7 20,726 + +1.3 20,726 + +0.4 20,726 + -1.0 20,767 + +0.5 20,786 - +1.8 20,786 - -0.6 20,814 + EX31_RS17355 0.13 -1.0 20,815 - EX31_RS17355 0.13 +2.9 20,989 + EX31_RS17355 0.44 +0.2 20,989 + EX31_RS17355 0.44 -0.2 20,989 + EX31_RS17355 0.44 +0.2 20,989 + EX31_RS17355 0.44 +0.8 20,989 + EX31_RS17355 0.44 +1.2 20,989 + EX31_RS17355 0.44 -0.2 21,051 + EX31_RS17355 0.55 -1.2 21,051 + EX31_RS17355 0.55 +0.6 21,102 + EX31_RS17355 0.64 +0.9 21,103 - EX31_RS17355 0.65 +0.9 21,103 - EX31_RS17355 0.65 +1.6 21,153 + EX31_RS17355 0.73 +0.9 21,153 + EX31_RS17355 0.73 +0.4 21,177 + EX31_RS17355 0.78 -0.7 21,177 + EX31_RS17355 0.78 -0.2 21,177 + EX31_RS17355 0.78 +1.3 21,177 + EX31_RS17355 0.78 -0.6 21,178 - EX31_RS17355 0.78 +1.1 21,178 - EX31_RS17355 0.78 -0.5 21,198 + EX31_RS17355 0.81 -0.3 21,198 + EX31_RS17355 0.81 +0.6 21,198 + EX31_RS17355 0.81 -1.0 21,198 + EX31_RS17355 0.81 +0.4 21,198 + EX31_RS17355 0.81 +0.4 21,199 - EX31_RS17355 0.82 +1.0 21,286 + +0.1 21,300 + +0.8 21,320 + -1.2 21,359 - -0.6 21,378 - -0.4 21,546 - EX31_RS17360 0.29 +0.7 21,546 - EX31_RS17360 0.29 +0.4 21,561 + EX31_RS17360 0.32 +1.3 21,562 - EX31_RS17360 0.32 -1.8 21,562 - EX31_RS17360 0.32 -1.3 21,562 - EX31_RS17360 0.32 -0.8 21,562 - EX31_RS17360 0.32 +1.0
Or see this region's nucleotide sequence