Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS16675 and EX31_RS16680 are separated by 55 nucleotides EX31_RS16680 and EX31_RS16685 overlap by 4 nucleotides EX31_RS16685 and EX31_RS16690 are separated by 15 nucleotides
EX31_RS16675: EX31_RS16675 - histidine utilization repressor, at 17,937 to 18,686
_RS16675
EX31_RS16680: EX31_RS16680 - enoyl-CoA hydratase, at 18,742 to 19,512
_RS16680
EX31_RS16685: EX31_RS16685 - 3-hydroxyacyl-CoA dehydrogenase family protein, at 19,509 to 20,402
_RS16685
EX31_RS16690: EX31_RS16690 - CoA transferase, at 20,418 to 21,620
_RS16690
Position (kb) 19
20
21 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 18.621 kb on - strand at 18.622 kb on + strand at 18.622 kb on + strand at 18.623 kb on - strand at 18.623 kb on - strand at 18.623 kb on - strand at 18.700 kb on + strand at 18.701 kb on - strand at 18.701 kb on - strand at 18.701 kb on - strand at 18.701 kb on - strand at 18.743 kb on + strand at 18.843 kb on - strand, within EX31_RS16680 at 19.280 kb on + strand, within EX31_RS16680 at 19.281 kb on - strand, within EX31_RS16680 at 19.281 kb on - strand, within EX31_RS16680 at 19.378 kb on - strand, within EX31_RS16680 at 19.778 kb on + strand, within EX31_RS16685 at 19.785 kb on + strand, within EX31_RS16685 at 19.858 kb on + strand, within EX31_RS16685 at 19.859 kb on - strand, within EX31_RS16685 at 19.859 kb on - strand, within EX31_RS16685 at 19.896 kb on - strand, within EX31_RS16685 at 19.956 kb on - strand, within EX31_RS16685 at 20.340 kb on + strand at 20.405 kb on + strand at 20.406 kb on - strand at 20.571 kb on - strand, within EX31_RS16690 at 20.955 kb on - strand, within EX31_RS16690 at 21.162 kb on - strand, within EX31_RS16690 at 21.193 kb on + strand, within EX31_RS16690 at 21.247 kb on + strand, within EX31_RS16690 at 21.247 kb on + strand, within EX31_RS16690 at 21.248 kb on - strand, within EX31_RS16690 at 21.248 kb on - strand, within EX31_RS16690 at 21.283 kb on + strand, within EX31_RS16690
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 18,621 - +0.5 18,622 + -1.0 18,622 + +1.1 18,623 - +1.4 18,623 - +4.8 18,623 - +0.4 18,700 + +0.1 18,701 - +0.4 18,701 - -0.7 18,701 - +3.0 18,701 - -1.2 18,743 + -1.0 18,843 - EX31_RS16680 0.13 +0.8 19,280 + EX31_RS16680 0.70 +1.8 19,281 - EX31_RS16680 0.70 +0.7 19,281 - EX31_RS16680 0.70 +0.1 19,378 - EX31_RS16680 0.82 +0.8 19,778 + EX31_RS16685 0.30 -0.7 19,785 + EX31_RS16685 0.31 -0.2 19,858 + EX31_RS16685 0.39 -0.0 19,859 - EX31_RS16685 0.39 +0.4 19,859 - EX31_RS16685 0.39 -1.6 19,896 - EX31_RS16685 0.43 -0.3 19,956 - EX31_RS16685 0.50 -0.2 20,340 + +1.0 20,405 + -0.5 20,406 - -1.0 20,571 - EX31_RS16690 0.13 +1.8 20,955 - EX31_RS16690 0.45 +0.4 21,162 - EX31_RS16690 0.62 +2.2 21,193 + EX31_RS16690 0.64 -1.0 21,247 + EX31_RS16690 0.69 +2.8 21,247 + EX31_RS16690 0.69 -0.7 21,248 - EX31_RS16690 0.69 +0.2 21,248 - EX31_RS16690 0.69 -0.2 21,283 + EX31_RS16690 0.72 +1.4
Or see this region's nucleotide sequence