Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12230 and EX31_RS12235 are separated by 355 nucleotides EX31_RS12235 and EX31_RS12240 are separated by 255 nucleotides
EX31_RS12230: EX31_RS12230 - RNase E specificity factor CsrD, at 112,208 to 114,142
_RS12230
EX31_RS12235: EX31_RS12235 - rod shape-determining protein, at 114,498 to 115,541
_RS12235
EX31_RS12240: EX31_RS12240 - rod shape-determining protein MreC, at 115,797 to 116,804
_RS12240
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 113.595 kb on + strand, within EX31_RS12230 at 113.595 kb on + strand, within EX31_RS12230 at 113.595 kb on + strand, within EX31_RS12230 at 113.595 kb on + strand, within EX31_RS12230 at 113.595 kb on + strand, within EX31_RS12230 at 113.596 kb on - strand, within EX31_RS12230 at 113.596 kb on - strand, within EX31_RS12230 at 113.596 kb on - strand, within EX31_RS12230 at 113.596 kb on - strand, within EX31_RS12230 at 113.605 kb on + strand, within EX31_RS12230 at 113.606 kb on - strand, within EX31_RS12230 at 113.613 kb on + strand, within EX31_RS12230 at 113.613 kb on + strand, within EX31_RS12230 at 113.614 kb on - strand, within EX31_RS12230 at 113.614 kb on - strand, within EX31_RS12230 at 113.617 kb on + strand, within EX31_RS12230 at 113.617 kb on + strand, within EX31_RS12230 at 113.618 kb on - strand, within EX31_RS12230 at 113.641 kb on - strand, within EX31_RS12230 at 113.668 kb on - strand, within EX31_RS12230 at 113.701 kb on + strand, within EX31_RS12230 at 113.776 kb on + strand, within EX31_RS12230 at 113.776 kb on + strand, within EX31_RS12230 at 113.776 kb on + strand, within EX31_RS12230 at 113.777 kb on - strand, within EX31_RS12230 at 113.777 kb on - strand, within EX31_RS12230 at 113.777 kb on - strand, within EX31_RS12230 at 113.809 kb on - strand, within EX31_RS12230 at 113.809 kb on - strand, within EX31_RS12230 at 113.870 kb on + strand, within EX31_RS12230 at 113.870 kb on + strand, within EX31_RS12230 at 113.870 kb on + strand, within EX31_RS12230 at 113.871 kb on - strand, within EX31_RS12230 at 113.871 kb on - strand, within EX31_RS12230 at 114.001 kb on - strand at 114.001 kb on - strand at 114.140 kb on + strand at 114.140 kb on + strand at 114.140 kb on + strand at 114.140 kb on + strand at 114.141 kb on - strand at 114.141 kb on - strand at 114.141 kb on - strand at 114.141 kb on - strand at 114.141 kb on - strand at 114.141 kb on - strand at 114.141 kb on - strand at 114.159 kb on + strand at 114.159 kb on + strand at 114.159 kb on + strand at 114.159 kb on + strand at 114.159 kb on + strand at 114.159 kb on + strand at 114.159 kb on + strand at 114.160 kb on - strand at 114.160 kb on - strand at 114.163 kb on + strand at 114.163 kb on + strand at 114.163 kb on + strand at 114.164 kb on - strand at 114.168 kb on + strand at 114.168 kb on + strand at 114.168 kb on + strand at 114.168 kb on + strand at 114.169 kb on - strand at 114.190 kb on + strand at 114.190 kb on + strand at 114.190 kb on + strand at 114.190 kb on + strand at 114.190 kb on + strand at 114.191 kb on - strand at 114.191 kb on - strand at 114.191 kb on - strand at 114.191 kb on - strand at 114.191 kb on - strand at 114.191 kb on - strand at 114.250 kb on + strand at 114.251 kb on - strand at 114.251 kb on - strand at 114.258 kb on + strand at 114.259 kb on - strand at 114.266 kb on + strand at 114.266 kb on + strand at 114.267 kb on - strand at 114.281 kb on + strand at 114.281 kb on + strand at 114.281 kb on + strand at 114.281 kb on + strand at 114.281 kb on + strand at 114.308 kb on - strand at 114.331 kb on + strand at 114.331 kb on + strand at 115.495 kb on + strand at 115.525 kb on + strand at 115.539 kb on + strand at 115.539 kb on + strand at 115.590 kb on + strand at 115.590 kb on + strand at 115.590 kb on + strand at 115.749 kb on + strand at 115.749 kb on + strand at 115.749 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 113,595 + EX31_RS12230 0.72 -1.9 113,595 + EX31_RS12230 0.72 -0.2 113,595 + EX31_RS12230 0.72 -1.0 113,595 + EX31_RS12230 0.72 -0.1 113,595 + EX31_RS12230 0.72 +0.1 113,596 - EX31_RS12230 0.72 -0.9 113,596 - EX31_RS12230 0.72 -1.5 113,596 - EX31_RS12230 0.72 -0.7 113,596 - EX31_RS12230 0.72 -0.7 113,605 + EX31_RS12230 0.72 -0.7 113,606 - EX31_RS12230 0.72 -0.4 113,613 + EX31_RS12230 0.73 -0.8 113,613 + EX31_RS12230 0.73 -0.3 113,614 - EX31_RS12230 0.73 -0.6 113,614 - EX31_RS12230 0.73 -1.2 113,617 + EX31_RS12230 0.73 -0.7 113,617 + EX31_RS12230 0.73 +0.3 113,618 - EX31_RS12230 0.73 +0.0 113,641 - EX31_RS12230 0.74 -1.1 113,668 - EX31_RS12230 0.75 -0.8 113,701 + EX31_RS12230 0.77 -1.7 113,776 + EX31_RS12230 0.81 -1.6 113,776 + EX31_RS12230 0.81 -0.8 113,776 + EX31_RS12230 0.81 +1.3 113,777 - EX31_RS12230 0.81 +0.3 113,777 - EX31_RS12230 0.81 +1.6 113,777 - EX31_RS12230 0.81 -0.0 113,809 - EX31_RS12230 0.83 -0.5 113,809 - EX31_RS12230 0.83 +1.5 113,870 + EX31_RS12230 0.86 -1.9 113,870 + EX31_RS12230 0.86 -1.4 113,870 + EX31_RS12230 0.86 -1.2 113,871 - EX31_RS12230 0.86 -0.5 113,871 - EX31_RS12230 0.86 -0.2 114,001 - -0.2 114,001 - +0.2 114,140 + -0.5 114,140 + +0.5 114,140 + +0.1 114,140 + -0.2 114,141 - -0.9 114,141 - -0.3 114,141 - -0.1 114,141 - +1.2 114,141 - +0.3 114,141 - -0.1 114,141 - +1.6 114,159 + +0.9 114,159 + -0.7 114,159 + +0.3 114,159 + -0.9 114,159 + +0.2 114,159 + -0.3 114,159 + +0.8 114,160 - +0.0 114,160 - +0.9 114,163 + -0.1 114,163 + +0.3 114,163 + +0.9 114,164 - -0.1 114,168 + +0.6 114,168 + +0.1 114,168 + +0.8 114,168 + -1.1 114,169 - -0.1 114,190 + +0.3 114,190 + +0.1 114,190 + +1.3 114,190 + -0.1 114,190 + -0.7 114,191 - -0.3 114,191 - +0.5 114,191 - +0.2 114,191 - -0.1 114,191 - -0.1 114,191 - -0.0 114,250 + -0.9 114,251 - +1.9 114,251 - -0.2 114,258 + +0.4 114,259 - -0.8 114,266 + +1.1 114,266 + -0.4 114,267 - +0.3 114,281 + +0.5 114,281 + -0.4 114,281 + +1.4 114,281 + +0.5 114,281 + -1.2 114,308 - -0.3 114,331 + +0.7 114,331 + -1.3 115,495 + -0.5 115,525 + +0.7 115,539 + -0.3 115,539 + +0.7 115,590 + -0.1 115,590 + -0.4 115,590 + -0.4 115,749 + -0.7 115,749 + +0.1 115,749 + +0.7
Or see this region's nucleotide sequence