Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12055 and EX31_RS12060 are separated by 85 nucleotides EX31_RS12060 and EX31_RS12065 are separated by 35 nucleotides EX31_RS12065 and EX31_RS12070 are separated by 165 nucleotides
EX31_RS12055: EX31_RS12055 - helix-turn-helix domain-containing protein, at 75,926 to 76,837
_RS12055
EX31_RS12060: EX31_RS12060 - GNAT family N-acetyltransferase, at 76,923 to 77,456
_RS12060
EX31_RS12065: EX31_RS12065 - glutathione S-transferase, at 77,492 to 78,115
_RS12065
EX31_RS12070: EX31_RS12070 - NAD-dependent epimerase/dehydratase family protein, at 78,281 to 79,396
_RS12070
Position (kb) 77
78
79 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 76.501 kb on - strand, within EX31_RS12055 at 76.522 kb on + strand, within EX31_RS12055 at 76.522 kb on + strand, within EX31_RS12055 at 76.544 kb on + strand, within EX31_RS12055 at 76.544 kb on + strand, within EX31_RS12055 at 76.545 kb on - strand, within EX31_RS12055 at 76.546 kb on + strand, within EX31_RS12055 at 76.547 kb on - strand, within EX31_RS12055 at 76.547 kb on - strand, within EX31_RS12055 at 76.572 kb on + strand, within EX31_RS12055 at 76.575 kb on + strand, within EX31_RS12055 at 76.576 kb on - strand, within EX31_RS12055 at 76.633 kb on + strand, within EX31_RS12055 at 76.634 kb on - strand, within EX31_RS12055 at 76.664 kb on + strand, within EX31_RS12055 at 76.664 kb on + strand, within EX31_RS12055 at 76.664 kb on + strand, within EX31_RS12055 at 76.664 kb on + strand, within EX31_RS12055 at 76.664 kb on + strand, within EX31_RS12055 at 76.664 kb on + strand, within EX31_RS12055 at 76.664 kb on + strand, within EX31_RS12055 at 76.665 kb on - strand, within EX31_RS12055 at 76.665 kb on - strand, within EX31_RS12055 at 76.665 kb on - strand, within EX31_RS12055 at 76.665 kb on - strand, within EX31_RS12055 at 76.665 kb on - strand, within EX31_RS12055 at 76.665 kb on - strand, within EX31_RS12055 at 76.724 kb on - strand, within EX31_RS12055 at 76.887 kb on + strand at 76.887 kb on + strand at 76.887 kb on + strand at 76.887 kb on + strand at 76.888 kb on - strand at 76.888 kb on - strand at 76.888 kb on - strand at 76.898 kb on + strand at 76.898 kb on + strand at 76.898 kb on + strand at 76.898 kb on + strand at 76.898 kb on + strand at 76.899 kb on - strand at 76.899 kb on - strand at 76.899 kb on - strand at 76.899 kb on - strand at 76.899 kb on - strand at 76.899 kb on - strand at 76.899 kb on - strand at 76.909 kb on - strand at 76.909 kb on - strand at 76.966 kb on + strand at 76.967 kb on - strand at 76.997 kb on + strand, within EX31_RS12060 at 76.997 kb on + strand, within EX31_RS12060 at 76.997 kb on + strand, within EX31_RS12060 at 76.998 kb on - strand, within EX31_RS12060 at 76.998 kb on - strand, within EX31_RS12060 at 77.034 kb on + strand, within EX31_RS12060 at 77.034 kb on + strand, within EX31_RS12060 at 77.034 kb on + strand, within EX31_RS12060 at 77.034 kb on + strand, within EX31_RS12060 at 77.034 kb on + strand, within EX31_RS12060 at 77.034 kb on + strand, within EX31_RS12060 at 77.035 kb on - strand, within EX31_RS12060 at 77.035 kb on - strand, within EX31_RS12060 at 77.121 kb on + strand, within EX31_RS12060 at 77.271 kb on + strand, within EX31_RS12060 at 77.313 kb on - strand, within EX31_RS12060 at 77.313 kb on - strand, within EX31_RS12060 at 77.332 kb on + strand, within EX31_RS12060 at 77.332 kb on + strand, within EX31_RS12060 at 77.332 kb on + strand, within EX31_RS12060 at 77.332 kb on + strand, within EX31_RS12060 at 77.332 kb on + strand, within EX31_RS12060 at 77.333 kb on - strand, within EX31_RS12060 at 77.333 kb on - strand, within EX31_RS12060 at 77.333 kb on - strand, within EX31_RS12060 at 77.333 kb on - strand, within EX31_RS12060 at 77.333 kb on - strand, within EX31_RS12060 at 77.342 kb on + strand, within EX31_RS12060 at 77.342 kb on + strand, within EX31_RS12060 at 77.415 kb on + strand at 77.415 kb on + strand at 77.416 kb on - strand at 77.416 kb on - strand at 77.416 kb on - strand at 77.442 kb on - strand at 77.471 kb on - strand at 77.471 kb on - strand at 77.509 kb on + strand at 77.509 kb on + strand at 77.533 kb on + strand at 77.533 kb on + strand at 77.533 kb on + strand at 77.676 kb on + strand, within EX31_RS12065 at 77.676 kb on + strand, within EX31_RS12065 at 77.676 kb on + strand, within EX31_RS12065 at 77.705 kb on + strand, within EX31_RS12065 at 77.806 kb on + strand, within EX31_RS12065 at 77.807 kb on - strand, within EX31_RS12065 at 77.905 kb on + strand, within EX31_RS12065 at 77.906 kb on - strand, within EX31_RS12065 at 77.945 kb on + strand, within EX31_RS12065 at 77.945 kb on + strand, within EX31_RS12065 at 77.946 kb on - strand, within EX31_RS12065 at 77.946 kb on - strand, within EX31_RS12065 at 77.965 kb on + strand, within EX31_RS12065 at 77.965 kb on + strand, within EX31_RS12065 at 78.053 kb on + strand at 78.053 kb on + strand at 78.053 kb on + strand at 78.054 kb on - strand at 78.057 kb on - strand at 78.092 kb on + strand at 78.093 kb on - strand at 78.189 kb on + strand at 78.189 kb on + strand at 78.189 kb on + strand at 78.189 kb on + strand at 78.189 kb on + strand at 78.189 kb on + strand at 78.189 kb on + strand at 78.189 kb on + strand at 78.190 kb on - strand at 78.190 kb on - strand at 78.190 kb on - strand at 78.190 kb on - strand at 78.190 kb on - strand at 78.190 kb on - strand at 78.190 kb on - strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.197 kb on + strand at 78.197 kb on + strand at 78.197 kb on + strand at 78.197 kb on + strand at 78.198 kb on - strand at 78.198 kb on - strand at 78.198 kb on - strand at 78.198 kb on - strand at 78.283 kb on - strand at 78.344 kb on - strand at 78.344 kb on - strand at 78.344 kb on - strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.352 kb on - strand at 78.352 kb on - strand at 78.352 kb on - strand at 78.359 kb on - strand at 78.390 kb on - strand at 78.390 kb on - strand at 78.390 kb on - strand at 78.473 kb on - strand, within EX31_RS12070 at 78.494 kb on - strand, within EX31_RS12070 at 78.562 kb on + strand, within EX31_RS12070 at 78.563 kb on - strand, within EX31_RS12070 at 78.563 kb on - strand, within EX31_RS12070 at 78.563 kb on - strand, within EX31_RS12070 at 78.565 kb on + strand, within EX31_RS12070 at 78.587 kb on - strand, within EX31_RS12070 at 78.587 kb on - strand, within EX31_RS12070 at 78.587 kb on - strand, within EX31_RS12070 at 78.591 kb on - strand, within EX31_RS12070 at 78.607 kb on + strand, within EX31_RS12070 at 78.631 kb on + strand, within EX31_RS12070 at 78.636 kb on + strand, within EX31_RS12070 at 78.693 kb on + strand, within EX31_RS12070 at 78.707 kb on - strand, within EX31_RS12070 at 78.751 kb on + strand, within EX31_RS12070 at 78.751 kb on + strand, within EX31_RS12070 at 78.820 kb on + strand, within EX31_RS12070 at 78.901 kb on + strand, within EX31_RS12070 at 78.901 kb on + strand, within EX31_RS12070 at 78.901 kb on + strand, within EX31_RS12070 at 78.901 kb on + strand, within EX31_RS12070 at 78.901 kb on + strand, within EX31_RS12070 at 78.901 kb on + strand, within EX31_RS12070 at 78.901 kb on + strand, within EX31_RS12070 at 78.901 kb on + strand, within EX31_RS12070 at 78.901 kb on + strand, within EX31_RS12070 at 78.901 kb on + strand, within EX31_RS12070 at 78.902 kb on - strand, within EX31_RS12070 at 78.902 kb on - strand, within EX31_RS12070 at 78.902 kb on - strand, within EX31_RS12070 at 78.902 kb on - strand, within EX31_RS12070 at 78.903 kb on + strand, within EX31_RS12070 at 78.903 kb on + strand, within EX31_RS12070 at 78.903 kb on + strand, within EX31_RS12070 at 78.903 kb on + strand, within EX31_RS12070 at 78.904 kb on - strand, within EX31_RS12070 at 78.904 kb on - strand, within EX31_RS12070 at 78.904 kb on - strand, within EX31_RS12070 at 78.904 kb on - strand, within EX31_RS12070 at 78.904 kb on - strand, within EX31_RS12070 at 78.904 kb on - strand, within EX31_RS12070 at 78.904 kb on - strand, within EX31_RS12070 at 78.948 kb on + strand, within EX31_RS12070 at 78.948 kb on + strand, within EX31_RS12070 at 78.949 kb on - strand, within EX31_RS12070 at 78.949 kb on - strand, within EX31_RS12070 at 78.949 kb on - strand, within EX31_RS12070 at 78.949 kb on - strand, within EX31_RS12070 at 79.030 kb on + strand, within EX31_RS12070 at 79.030 kb on + strand, within EX31_RS12070 at 79.030 kb on + strand, within EX31_RS12070 at 79.030 kb on + strand, within EX31_RS12070 at 79.031 kb on - strand, within EX31_RS12070 at 79.031 kb on - strand, within EX31_RS12070 at 79.031 kb on - strand, within EX31_RS12070 at 79.031 kb on - strand, within EX31_RS12070 at 79.031 kb on - strand, within EX31_RS12070 at 79.031 kb on - strand, within EX31_RS12070 at 79.031 kb on - strand, within EX31_RS12070 at 79.031 kb on - strand, within EX31_RS12070 at 79.031 kb on - strand, within EX31_RS12070 at 79.031 kb on - strand, within EX31_RS12070 at 79.031 kb on - strand, within EX31_RS12070 at 79.115 kb on - strand, within EX31_RS12070 at 79.115 kb on - strand, within EX31_RS12070 at 79.115 kb on - strand, within EX31_RS12070
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 76,501 - EX31_RS12055 0.63 +0.6 76,522 + EX31_RS12055 0.65 -0.0 76,522 + EX31_RS12055 0.65 +0.1 76,544 + EX31_RS12055 0.68 +0.0 76,544 + EX31_RS12055 0.68 +0.1 76,545 - EX31_RS12055 0.68 -0.9 76,546 + EX31_RS12055 0.68 +1.3 76,547 - EX31_RS12055 0.68 +0.1 76,547 - EX31_RS12055 0.68 +0.5 76,572 + EX31_RS12055 0.71 -0.2 76,575 + EX31_RS12055 0.71 +0.5 76,576 - EX31_RS12055 0.71 +0.6 76,633 + EX31_RS12055 0.78 -0.3 76,634 - EX31_RS12055 0.78 -1.7 76,664 + EX31_RS12055 0.81 -0.5 76,664 + EX31_RS12055 0.81 +0.1 76,664 + EX31_RS12055 0.81 -0.3 76,664 + EX31_RS12055 0.81 -0.9 76,664 + EX31_RS12055 0.81 +0.5 76,664 + EX31_RS12055 0.81 -0.2 76,664 + EX31_RS12055 0.81 +0.2 76,665 - EX31_RS12055 0.81 -0.2 76,665 - EX31_RS12055 0.81 -0.9 76,665 - EX31_RS12055 0.81 +1.1 76,665 - EX31_RS12055 0.81 -0.1 76,665 - EX31_RS12055 0.81 +0.1 76,665 - EX31_RS12055 0.81 +0.2 76,724 - EX31_RS12055 0.88 -0.2 76,887 + -0.0 76,887 + +0.1 76,887 + -0.5 76,887 + -0.1 76,888 - +1.3 76,888 - -0.2 76,888 - -0.7 76,898 + +0.4 76,898 + +1.1 76,898 + +0.6 76,898 + -0.5 76,898 + +0.4 76,899 - +0.7 76,899 - +1.8 76,899 - +0.0 76,899 - -0.3 76,899 - -0.2 76,899 - -0.0 76,899 - +0.3 76,909 - +0.4 76,909 - -0.9 76,966 + +0.9 76,967 - -0.3 76,997 + EX31_RS12060 0.14 -0.2 76,997 + EX31_RS12060 0.14 +3.0 76,997 + EX31_RS12060 0.14 +0.3 76,998 - EX31_RS12060 0.14 +0.2 76,998 - EX31_RS12060 0.14 -0.2 77,034 + EX31_RS12060 0.21 +1.0 77,034 + EX31_RS12060 0.21 -1.1 77,034 + EX31_RS12060 0.21 -0.2 77,034 + EX31_RS12060 0.21 +0.7 77,034 + EX31_RS12060 0.21 +1.0 77,034 + EX31_RS12060 0.21 -1.0 77,035 - EX31_RS12060 0.21 +1.2 77,035 - EX31_RS12060 0.21 -0.5 77,121 + EX31_RS12060 0.37 +0.8 77,271 + EX31_RS12060 0.65 +0.1 77,313 - EX31_RS12060 0.73 +0.4 77,313 - EX31_RS12060 0.73 +1.2 77,332 + EX31_RS12060 0.77 +1.7 77,332 + EX31_RS12060 0.77 +0.1 77,332 + EX31_RS12060 0.77 -0.5 77,332 + EX31_RS12060 0.77 -0.0 77,332 + EX31_RS12060 0.77 +0.5 77,333 - EX31_RS12060 0.77 +0.4 77,333 - EX31_RS12060 0.77 -0.3 77,333 - EX31_RS12060 0.77 -1.7 77,333 - EX31_RS12060 0.77 -0.3 77,333 - EX31_RS12060 0.77 -0.6 77,342 + EX31_RS12060 0.78 +0.1 77,342 + EX31_RS12060 0.78 +0.7 77,415 + -0.3 77,415 + -0.2 77,416 - +1.1 77,416 - +0.5 77,416 - -0.1 77,442 - +0.4 77,471 - +0.3 77,471 - +0.2 77,509 + -0.0 77,509 + +0.7 77,533 + +0.1 77,533 + -1.0 77,533 + +0.3 77,676 + EX31_RS12065 0.29 +0.0 77,676 + EX31_RS12065 0.29 -0.4 77,676 + EX31_RS12065 0.29 -2.5 77,705 + EX31_RS12065 0.34 -0.6 77,806 + EX31_RS12065 0.50 +0.1 77,807 - EX31_RS12065 0.50 +0.4 77,905 + EX31_RS12065 0.66 -0.8 77,906 - EX31_RS12065 0.66 -1.4 77,945 + EX31_RS12065 0.73 -0.0 77,945 + EX31_RS12065 0.73 -0.2 77,946 - EX31_RS12065 0.73 -0.6 77,946 - EX31_RS12065 0.73 -0.6 77,965 + EX31_RS12065 0.76 -0.2 77,965 + EX31_RS12065 0.76 +3.3 78,053 + -0.8 78,053 + -0.0 78,053 + +0.9 78,054 - +0.4 78,057 - +0.9 78,092 + +1.1 78,093 - -0.3 78,189 + -0.0 78,189 + -1.3 78,189 + -0.3 78,189 + -0.4 78,189 + +0.4 78,189 + +0.2 78,189 + +0.7 78,189 + -1.8 78,190 - +0.9 78,190 - -0.9 78,190 - +1.9 78,190 - -0.3 78,190 - -0.3 78,190 - +0.4 78,190 - -0.3 78,193 + +0.6 78,193 + +0.3 78,193 + -1.6 78,193 + +0.3 78,193 + +0.2 78,193 + -0.2 78,193 + +0.4 78,193 + -1.0 78,193 + +1.1 78,193 + +0.4 78,193 + +0.5 78,193 + +1.3 78,194 - +1.8 78,194 - +0.4 78,194 - +1.5 78,194 - +0.6 78,194 - -0.5 78,194 - -0.6 78,194 - +1.3 78,194 - -0.3 78,194 - +0.1 78,197 + +1.0 78,197 + +0.7 78,197 + -0.5 78,197 + +1.7 78,198 - -1.0 78,198 - -0.9 78,198 - +0.0 78,198 - -0.0 78,283 - -0.0 78,344 - -0.2 78,344 - +0.3 78,344 - +0.1 78,351 + +1.1 78,351 + +0.7 78,351 + +0.3 78,351 + +0.3 78,351 + +0.5 78,351 + -0.2 78,352 - -2.3 78,352 - -0.2 78,352 - +0.0 78,359 - -1.0 78,390 - +0.3 78,390 - -0.3 78,390 - +0.6 78,473 - EX31_RS12070 0.17 -1.3 78,494 - EX31_RS12070 0.19 +1.0 78,562 + EX31_RS12070 0.25 -0.0 78,563 - EX31_RS12070 0.25 -0.4 78,563 - EX31_RS12070 0.25 -0.8 78,563 - EX31_RS12070 0.25 +0.8 78,565 + EX31_RS12070 0.25 +0.7 78,587 - EX31_RS12070 0.27 +0.7 78,587 - EX31_RS12070 0.27 -0.7 78,587 - EX31_RS12070 0.27 +1.1 78,591 - EX31_RS12070 0.28 +0.7 78,607 + EX31_RS12070 0.29 -0.3 78,631 + EX31_RS12070 0.31 +0.0 78,636 + EX31_RS12070 0.32 -0.0 78,693 + EX31_RS12070 0.37 +0.2 78,707 - EX31_RS12070 0.38 -1.2 78,751 + EX31_RS12070 0.42 +1.4 78,751 + EX31_RS12070 0.42 -0.7 78,820 + EX31_RS12070 0.48 +0.1 78,901 + EX31_RS12070 0.56 +0.7 78,901 + EX31_RS12070 0.56 -0.7 78,901 + EX31_RS12070 0.56 -1.0 78,901 + EX31_RS12070 0.56 +1.3 78,901 + EX31_RS12070 0.56 +0.3 78,901 + EX31_RS12070 0.56 -0.0 78,901 + EX31_RS12070 0.56 -0.2 78,901 + EX31_RS12070 0.56 -1.2 78,901 + EX31_RS12070 0.56 +2.2 78,901 + EX31_RS12070 0.56 +1.4 78,902 - EX31_RS12070 0.56 -0.6 78,902 - EX31_RS12070 0.56 -0.9 78,902 - EX31_RS12070 0.56 +0.1 78,902 - EX31_RS12070 0.56 -1.2 78,903 + EX31_RS12070 0.56 -0.5 78,903 + EX31_RS12070 0.56 -0.0 78,903 + EX31_RS12070 0.56 +0.1 78,903 + EX31_RS12070 0.56 +0.0 78,904 - EX31_RS12070 0.56 -0.5 78,904 - EX31_RS12070 0.56 +0.1 78,904 - EX31_RS12070 0.56 -0.9 78,904 - EX31_RS12070 0.56 -0.4 78,904 - EX31_RS12070 0.56 -0.0 78,904 - EX31_RS12070 0.56 -0.1 78,904 - EX31_RS12070 0.56 -0.2 78,948 + EX31_RS12070 0.60 +0.1 78,948 + EX31_RS12070 0.60 +0.2 78,949 - EX31_RS12070 0.60 +0.1 78,949 - EX31_RS12070 0.60 -0.4 78,949 - EX31_RS12070 0.60 +0.9 78,949 - EX31_RS12070 0.60 -0.6 79,030 + EX31_RS12070 0.67 +0.7 79,030 + EX31_RS12070 0.67 -0.8 79,030 + EX31_RS12070 0.67 -1.3 79,030 + EX31_RS12070 0.67 +0.1 79,031 - EX31_RS12070 0.67 -1.0 79,031 - EX31_RS12070 0.67 -1.0 79,031 - EX31_RS12070 0.67 -0.7 79,031 - EX31_RS12070 0.67 -0.6 79,031 - EX31_RS12070 0.67 -1.4 79,031 - EX31_RS12070 0.67 +0.5 79,031 - EX31_RS12070 0.67 +0.9 79,031 - EX31_RS12070 0.67 -0.2 79,031 - EX31_RS12070 0.67 -0.5 79,031 - EX31_RS12070 0.67 +0.2 79,031 - EX31_RS12070 0.67 -0.2 79,115 - EX31_RS12070 0.75 +0.4 79,115 - EX31_RS12070 0.75 -0.6 79,115 - EX31_RS12070 0.75 -1.0
Or see this region's nucleotide sequence