Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11340 and EX31_RS11345 overlap by 8 nucleotides EX31_RS11345 and EX31_RS11350 are separated by 325 nucleotides EX31_RS11350 and EX31_RS11355 are separated by 10 nucleotides
EX31_RS11340: EX31_RS11340 - flagellar biosynthesis protein FlhA, at 198,254 to 200,338
_RS11340
EX31_RS11345: EX31_RS11345 - flagellar type III secretion system protein FlhB, at 200,331 to 201,485
_RS11345
EX31_RS11350: EX31_RS11350 - protein phosphatase CheZ, at 201,811 to 202,455
_RS11350
EX31_RS11355: EX31_RS11355 - chemotaxis protein CheY, at 202,466 to 202,855
_RS11355
Position (kb) 200
201
202 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 199.556 kb on - strand, within EX31_RS11340 at 199.562 kb on - strand, within EX31_RS11340 at 199.615 kb on - strand, within EX31_RS11340 at 199.615 kb on - strand, within EX31_RS11340 at 199.615 kb on - strand, within EX31_RS11340 at 199.755 kb on + strand, within EX31_RS11340 at 199.756 kb on - strand, within EX31_RS11340 at 199.897 kb on + strand, within EX31_RS11340 at 199.897 kb on + strand, within EX31_RS11340 at 199.897 kb on + strand, within EX31_RS11340 at 199.898 kb on - strand, within EX31_RS11340 at 199.898 kb on - strand, within EX31_RS11340 at 199.898 kb on - strand, within EX31_RS11340 at 199.898 kb on - strand, within EX31_RS11340 at 200.090 kb on + strand, within EX31_RS11340 at 200.091 kb on - strand, within EX31_RS11340 at 200.297 kb on - strand at 200.332 kb on + strand at 200.385 kb on + strand at 200.477 kb on - strand, within EX31_RS11345 at 200.477 kb on - strand, within EX31_RS11345 at 200.650 kb on + strand, within EX31_RS11345 at 200.651 kb on - strand, within EX31_RS11345 at 200.651 kb on - strand, within EX31_RS11345 at 200.669 kb on - strand, within EX31_RS11345 at 200.792 kb on + strand, within EX31_RS11345 at 200.793 kb on - strand, within EX31_RS11345 at 200.987 kb on + strand, within EX31_RS11345 at 200.988 kb on - strand, within EX31_RS11345 at 200.998 kb on + strand, within EX31_RS11345 at 200.998 kb on + strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 200.999 kb on - strand, within EX31_RS11345 at 201.075 kb on + strand, within EX31_RS11345 at 201.132 kb on - strand, within EX31_RS11345 at 201.290 kb on + strand, within EX31_RS11345 at 201.291 kb on - strand, within EX31_RS11345 at 201.291 kb on - strand, within EX31_RS11345 at 201.291 kb on - strand, within EX31_RS11345 at 201.517 kb on + strand at 201.517 kb on + strand at 201.518 kb on - strand at 201.568 kb on + strand at 201.568 kb on + strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.569 kb on - strand at 201.606 kb on - strand at 201.638 kb on + strand at 201.660 kb on + strand at 201.661 kb on - strand at 201.661 kb on - strand at 201.696 kb on + strand at 201.716 kb on + strand at 201.791 kb on - strand at 201.842 kb on - strand at 202.074 kb on + strand, within EX31_RS11350 at 202.107 kb on - strand, within EX31_RS11350 at 202.286 kb on + strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.287 kb on - strand, within EX31_RS11350 at 202.377 kb on + strand, within EX31_RS11350 at 202.378 kb on - strand, within EX31_RS11350 at 202.444 kb on + strand at 202.445 kb on - strand at 202.445 kb on - strand at 202.445 kb on - strand at 202.456 kb on - strand at 202.456 kb on - strand at 202.456 kb on - strand at 202.457 kb on + strand at 202.457 kb on + strand at 202.458 kb on - strand at 202.458 kb on - strand at 202.458 kb on - strand at 202.458 kb on - strand at 202.458 kb on - strand at 202.458 kb on - strand at 202.458 kb on - strand at 202.458 kb on - strand at 202.467 kb on + strand at 202.468 kb on - strand at 202.468 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 199,556 - EX31_RS11340 0.62 -3.2 199,562 - EX31_RS11340 0.63 -1.6 199,615 - EX31_RS11340 0.65 -0.2 199,615 - EX31_RS11340 0.65 -0.2 199,615 - EX31_RS11340 0.65 -3.0 199,755 + EX31_RS11340 0.72 -0.9 199,756 - EX31_RS11340 0.72 -0.1 199,897 + EX31_RS11340 0.79 +0.5 199,897 + EX31_RS11340 0.79 -0.4 199,897 + EX31_RS11340 0.79 +2.1 199,898 - EX31_RS11340 0.79 -0.8 199,898 - EX31_RS11340 0.79 -2.9 199,898 - EX31_RS11340 0.79 -1.7 199,898 - EX31_RS11340 0.79 -0.8 200,090 + EX31_RS11340 0.88 -2.9 200,091 - EX31_RS11340 0.88 +0.1 200,297 - -1.0 200,332 + +0.5 200,385 + -1.5 200,477 - EX31_RS11345 0.13 -0.7 200,477 - EX31_RS11345 0.13 +1.1 200,650 + EX31_RS11345 0.28 -1.7 200,651 - EX31_RS11345 0.28 -0.3 200,651 - EX31_RS11345 0.28 -2.5 200,669 - EX31_RS11345 0.29 +0.5 200,792 + EX31_RS11345 0.40 +0.7 200,793 - EX31_RS11345 0.40 -0.5 200,987 + EX31_RS11345 0.57 -0.3 200,988 - EX31_RS11345 0.57 +1.0 200,998 + EX31_RS11345 0.58 -1.4 200,998 + EX31_RS11345 0.58 -0.8 200,999 - EX31_RS11345 0.58 +1.5 200,999 - EX31_RS11345 0.58 -0.7 200,999 - EX31_RS11345 0.58 -0.3 200,999 - EX31_RS11345 0.58 -0.8 200,999 - EX31_RS11345 0.58 -0.5 200,999 - EX31_RS11345 0.58 +0.1 200,999 - EX31_RS11345 0.58 -0.0 200,999 - EX31_RS11345 0.58 -0.8 200,999 - EX31_RS11345 0.58 -0.7 200,999 - EX31_RS11345 0.58 -1.1 201,075 + EX31_RS11345 0.64 +0.5 201,132 - EX31_RS11345 0.69 -2.0 201,290 + EX31_RS11345 0.83 +1.3 201,291 - EX31_RS11345 0.83 +0.0 201,291 - EX31_RS11345 0.83 +1.5 201,291 - EX31_RS11345 0.83 -0.9 201,517 + -0.2 201,517 + +0.5 201,518 - +0.1 201,568 + +1.1 201,568 + -0.3 201,569 - +0.9 201,569 - +0.3 201,569 - -0.6 201,569 - -1.8 201,569 - +0.1 201,569 - +0.1 201,569 - -0.2 201,569 - -0.2 201,569 - +0.9 201,606 - +1.4 201,638 + +0.1 201,660 + -0.1 201,661 - -1.7 201,661 - +0.5 201,696 + -1.1 201,716 + -0.5 201,791 - -0.7 201,842 - -0.4 202,074 + EX31_RS11350 0.41 -1.2 202,107 - EX31_RS11350 0.46 -0.4 202,286 + EX31_RS11350 0.74 +2.1 202,287 - EX31_RS11350 0.74 -0.6 202,287 - EX31_RS11350 0.74 +0.5 202,287 - EX31_RS11350 0.74 +0.1 202,287 - EX31_RS11350 0.74 +2.4 202,287 - EX31_RS11350 0.74 -0.5 202,287 - EX31_RS11350 0.74 -1.4 202,287 - EX31_RS11350 0.74 +2.7 202,287 - EX31_RS11350 0.74 -1.2 202,287 - EX31_RS11350 0.74 -1.3 202,377 + EX31_RS11350 0.88 -0.4 202,378 - EX31_RS11350 0.88 +0.3 202,444 + -0.2 202,445 - +1.4 202,445 - +3.4 202,445 - -1.0 202,456 - +0.1 202,456 - +0.5 202,456 - +0.1 202,457 + +0.2 202,457 + -1.5 202,458 - +0.8 202,458 - -2.3 202,458 - -0.2 202,458 - -0.2 202,458 - +0.6 202,458 - +1.8 202,458 - -1.0 202,458 - +0.9 202,467 + -2.7 202,468 - -1.6 202,468 - +1.3
Or see this region's nucleotide sequence