Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10835 and EX31_RS10840 overlap by 1 nucleotides EX31_RS10840 and EX31_RS10845 overlap by 4 nucleotides
EX31_RS10835: EX31_RS10835 - TolC family protein, at 87,645 to 88,898
_RS10835
EX31_RS10840: EX31_RS10840 - efflux RND transporter periplasmic adaptor subunit, at 88,898 to 90,142
_RS10840
EX31_RS10845: EX31_RS10845 - efflux RND transporter permease subunit, at 90,139 to 93,270
_RS10845
Position (kb)
88
89
90
91 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 87.948 kb on + strand, within EX31_RS10835 at 87.949 kb on - strand, within EX31_RS10835 at 87.949 kb on - strand, within EX31_RS10835 at 88.106 kb on - strand, within EX31_RS10835 at 88.386 kb on - strand, within EX31_RS10835 at 88.497 kb on + strand, within EX31_RS10835 at 88.497 kb on + strand, within EX31_RS10835 at 88.562 kb on + strand, within EX31_RS10835 at 88.709 kb on + strand, within EX31_RS10835 at 88.709 kb on + strand, within EX31_RS10835 at 88.872 kb on + strand at 89.033 kb on + strand, within EX31_RS10840 at 89.040 kb on - strand, within EX31_RS10840 at 89.093 kb on - strand, within EX31_RS10840 at 89.228 kb on - strand, within EX31_RS10840 at 89.234 kb on + strand, within EX31_RS10840 at 89.396 kb on + strand, within EX31_RS10840 at 89.396 kb on + strand, within EX31_RS10840 at 89.549 kb on + strand, within EX31_RS10840 at 89.550 kb on - strand, within EX31_RS10840 at 89.551 kb on + strand, within EX31_RS10840 at 89.551 kb on + strand, within EX31_RS10840 at 89.552 kb on - strand, within EX31_RS10840 at 89.552 kb on - strand, within EX31_RS10840 at 89.552 kb on - strand, within EX31_RS10840 at 89.692 kb on + strand, within EX31_RS10840 at 89.834 kb on + strand, within EX31_RS10840 at 89.834 kb on + strand, within EX31_RS10840 at 89.921 kb on - strand, within EX31_RS10840 at 90.413 kb on - strand at 90.413 kb on - strand at 90.413 kb on - strand at 90.418 kb on + strand at 90.418 kb on + strand at 90.418 kb on + strand at 90.418 kb on + strand at 90.419 kb on - strand at 90.556 kb on + strand, within EX31_RS10845 at 90.556 kb on + strand, within EX31_RS10845 at 90.557 kb on - strand, within EX31_RS10845 at 90.563 kb on - strand, within EX31_RS10845 at 90.635 kb on - strand, within EX31_RS10845 at 90.697 kb on + strand, within EX31_RS10845 at 90.697 kb on + strand, within EX31_RS10845 at 90.823 kb on + strand, within EX31_RS10845 at 90.824 kb on - strand, within EX31_RS10845 at 90.824 kb on - strand, within EX31_RS10845 at 91.058 kb on + strand, within EX31_RS10845 at 91.108 kb on + strand, within EX31_RS10845
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 87,948 + EX31_RS10835 0.24 -0.1 87,949 - EX31_RS10835 0.24 +0.7 87,949 - EX31_RS10835 0.24 -0.2 88,106 - EX31_RS10835 0.37 +0.3 88,386 - EX31_RS10835 0.59 -0.0 88,497 + EX31_RS10835 0.68 +0.8 88,497 + EX31_RS10835 0.68 +1.8 88,562 + EX31_RS10835 0.73 -1.4 88,709 + EX31_RS10835 0.85 +1.1 88,709 + EX31_RS10835 0.85 +1.6 88,872 + -0.1 89,033 + EX31_RS10840 0.11 +1.4 89,040 - EX31_RS10840 0.11 -1.4 89,093 - EX31_RS10840 0.16 +1.0 89,228 - EX31_RS10840 0.27 +1.4 89,234 + EX31_RS10840 0.27 +0.9 89,396 + EX31_RS10840 0.40 -0.0 89,396 + EX31_RS10840 0.40 +2.5 89,549 + EX31_RS10840 0.52 +0.5 89,550 - EX31_RS10840 0.52 -0.5 89,551 + EX31_RS10840 0.52 -1.4 89,551 + EX31_RS10840 0.52 +0.9 89,552 - EX31_RS10840 0.53 +0.5 89,552 - EX31_RS10840 0.53 +0.6 89,552 - EX31_RS10840 0.53 +1.5 89,692 + EX31_RS10840 0.64 +0.6 89,834 + EX31_RS10840 0.75 -0.1 89,834 + EX31_RS10840 0.75 +3.7 89,921 - EX31_RS10840 0.82 +1.3 90,413 - +0.3 90,413 - -0.5 90,413 - +2.9 90,418 + -0.5 90,418 + +0.4 90,418 + +1.1 90,418 + -0.9 90,419 - +0.9 90,556 + EX31_RS10845 0.13 +0.2 90,556 + EX31_RS10845 0.13 -0.4 90,557 - EX31_RS10845 0.13 +0.1 90,563 - EX31_RS10845 0.14 -1.8 90,635 - EX31_RS10845 0.16 +0.3 90,697 + EX31_RS10845 0.18 +0.4 90,697 + EX31_RS10845 0.18 +0.7 90,823 + EX31_RS10845 0.22 -1.4 90,824 - EX31_RS10845 0.22 +1.6 90,824 - EX31_RS10845 0.22 -0.7 91,058 + EX31_RS10845 0.29 +1.2 91,108 + EX31_RS10845 0.31 +0.1
Or see this region's nucleotide sequence