Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09375 and EX31_RS09380 are separated by 350 nucleotides EX31_RS09380 and EX31_RS09385 are separated by 471 nucleotides
EX31_RS09375: EX31_RS09375 - gamma-glutamylcyclotransferase, at 79,196 to 79,885
_RS09375
EX31_RS09380: EX31_RS09380 - putative cation transport regulator ChaB, at 80,236 to 80,469
_RS09380
EX31_RS09385: EX31_RS09385 - sodium-potassium/proton antiporter ChaA, at 80,941 to 82,041
_RS09385
Position (kb) 80
81 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 79.247 kb on + strand at 79.248 kb on - strand at 79.291 kb on - strand, within EX31_RS09375 at 79.324 kb on - strand, within EX31_RS09375 at 79.401 kb on + strand, within EX31_RS09375 at 79.647 kb on - strand, within EX31_RS09375 at 79.647 kb on - strand, within EX31_RS09375 at 79.666 kb on - strand, within EX31_RS09375 at 79.815 kb on - strand, within EX31_RS09375 at 79.881 kb on - strand at 79.881 kb on - strand at 79.902 kb on - strand at 79.909 kb on - strand at 79.909 kb on - strand at 79.965 kb on + strand at 79.966 kb on - strand at 79.966 kb on - strand at 80.018 kb on - strand at 80.018 kb on - strand at 80.033 kb on - strand at 80.035 kb on - strand at 80.035 kb on - strand at 80.055 kb on + strand at 80.144 kb on - strand at 80.147 kb on + strand at 80.235 kb on - strand at 80.248 kb on - strand at 80.248 kb on - strand at 80.277 kb on + strand, within EX31_RS09380 at 80.278 kb on - strand, within EX31_RS09380 at 80.310 kb on + strand, within EX31_RS09380 at 80.311 kb on - strand, within EX31_RS09380 at 80.311 kb on - strand, within EX31_RS09380 at 80.352 kb on + strand, within EX31_RS09380 at 80.364 kb on - strand, within EX31_RS09380 at 80.394 kb on + strand, within EX31_RS09380 at 80.395 kb on - strand, within EX31_RS09380 at 80.396 kb on + strand, within EX31_RS09380 at 80.396 kb on + strand, within EX31_RS09380 at 80.396 kb on + strand, within EX31_RS09380 at 80.397 kb on - strand, within EX31_RS09380 at 80.397 kb on - strand, within EX31_RS09380 at 80.397 kb on - strand, within EX31_RS09380 at 80.397 kb on - strand, within EX31_RS09380 at 80.397 kb on - strand, within EX31_RS09380 at 80.544 kb on - strand at 80.551 kb on - strand at 80.782 kb on - strand at 80.843 kb on - strand at 80.910 kb on + strand at 80.911 kb on - strand at 80.922 kb on + strand at 80.922 kb on + strand at 80.924 kb on + strand at 80.933 kb on + strand at 80.989 kb on + strand at 80.990 kb on - strand at 80.990 kb on - strand at 80.990 kb on - strand at 81.018 kb on + strand at 81.018 kb on + strand at 81.018 kb on + strand at 81.018 kb on + strand at 81.019 kb on - strand at 81.019 kb on - strand at 81.049 kb on - strand at 81.135 kb on - strand, within EX31_RS09385 at 81.135 kb on - strand, within EX31_RS09385 at 81.302 kb on + strand, within EX31_RS09385 at 81.346 kb on + strand, within EX31_RS09385 at 81.346 kb on + strand, within EX31_RS09385 at 81.346 kb on + strand, within EX31_RS09385 at 81.347 kb on - strand, within EX31_RS09385 at 81.347 kb on - strand, within EX31_RS09385 at 81.347 kb on - strand, within EX31_RS09385 at 81.347 kb on - strand, within EX31_RS09385 at 81.347 kb on - strand, within EX31_RS09385 at 81.373 kb on + strand, within EX31_RS09385 at 81.373 kb on + strand, within EX31_RS09385 at 81.373 kb on + strand, within EX31_RS09385 at 81.373 kb on + strand, within EX31_RS09385 at 81.373 kb on + strand, within EX31_RS09385 at 81.373 kb on + strand, within EX31_RS09385 at 81.452 kb on + strand, within EX31_RS09385
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 79,247 + +0.1 79,248 - +0.9 79,291 - EX31_RS09375 0.14 +1.0 79,324 - EX31_RS09375 0.19 +1.6 79,401 + EX31_RS09375 0.30 +0.1 79,647 - EX31_RS09375 0.65 +1.1 79,647 - EX31_RS09375 0.65 +0.5 79,666 - EX31_RS09375 0.68 +0.1 79,815 - EX31_RS09375 0.90 +0.7 79,881 - -1.9 79,881 - +0.2 79,902 - +0.9 79,909 - -0.6 79,909 - +1.2 79,965 + -0.9 79,966 - +0.3 79,966 - +0.3 80,018 - +1.3 80,018 - +0.3 80,033 - -0.6 80,035 - +0.4 80,035 - +0.3 80,055 + +0.5 80,144 - -1.0 80,147 + -2.8 80,235 - +0.3 80,248 - +1.0 80,248 - -1.8 80,277 + EX31_RS09380 0.18 +1.1 80,278 - EX31_RS09380 0.18 +1.0 80,310 + EX31_RS09380 0.32 +1.6 80,311 - EX31_RS09380 0.32 -0.9 80,311 - EX31_RS09380 0.32 -0.0 80,352 + EX31_RS09380 0.50 +0.7 80,364 - EX31_RS09380 0.55 +0.9 80,394 + EX31_RS09380 0.68 +0.6 80,395 - EX31_RS09380 0.68 -0.7 80,396 + EX31_RS09380 0.68 +0.6 80,396 + EX31_RS09380 0.68 -0.1 80,396 + EX31_RS09380 0.68 +2.3 80,397 - EX31_RS09380 0.69 +1.3 80,397 - EX31_RS09380 0.69 -0.9 80,397 - EX31_RS09380 0.69 -0.3 80,397 - EX31_RS09380 0.69 +1.4 80,397 - EX31_RS09380 0.69 +0.0 80,544 - -1.6 80,551 - -0.4 80,782 - -2.2 80,843 - -1.0 80,910 + +0.6 80,911 - +0.6 80,922 + +0.5 80,922 + +0.2 80,924 + +0.3 80,933 + -1.9 80,989 + -0.7 80,990 - +1.9 80,990 - -2.5 80,990 - +1.4 81,018 + -0.4 81,018 + -0.1 81,018 + +0.7 81,018 + +1.1 81,019 - -0.4 81,019 - -0.1 81,049 - -0.4 81,135 - EX31_RS09385 0.18 -0.5 81,135 - EX31_RS09385 0.18 +1.1 81,302 + EX31_RS09385 0.33 -0.4 81,346 + EX31_RS09385 0.37 +0.6 81,346 + EX31_RS09385 0.37 +0.3 81,346 + EX31_RS09385 0.37 +0.3 81,347 - EX31_RS09385 0.37 -0.9 81,347 - EX31_RS09385 0.37 -1.5 81,347 - EX31_RS09385 0.37 -0.9 81,347 - EX31_RS09385 0.37 -0.9 81,347 - EX31_RS09385 0.37 +0.4 81,373 + EX31_RS09385 0.39 -0.1 81,373 + EX31_RS09385 0.39 +0.3 81,373 + EX31_RS09385 0.39 +0.9 81,373 + EX31_RS09385 0.39 +1.9 81,373 + EX31_RS09385 0.39 +0.7 81,373 + EX31_RS09385 0.39 -0.4 81,452 + EX31_RS09385 0.46 -0.4
Or see this region's nucleotide sequence