Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS07170 and EX31_RS07175 are separated by 46 nucleotides EX31_RS07175 and EX31_RS07180 are separated by 114 nucleotides EX31_RS07180 and EX31_RS07185 are separated by 214 nucleotides
EX31_RS07170: EX31_RS07170 - glycogen debranching protein GlgX, at 229,657 to 231,939
_RS07170
EX31_RS07175: EX31_RS07175 - FAD:protein FMN transferase ApbE, at 231,986 to 233,026
_RS07175
EX31_RS07180: EX31_RS07180 - DNA endonuclease SmrA, at 233,141 to 233,707
_RS07180
EX31_RS07185: EX31_RS07185 - mannosyl-3-phosphoglycerate phosphatase-related protein, at 233,922 to 234,737
_RS07185
Position (kb)
231
232
233
234 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 231.148 kb on + strand, within EX31_RS07170 at 231.208 kb on + strand, within EX31_RS07170 at 231.208 kb on + strand, within EX31_RS07170 at 231.280 kb on + strand, within EX31_RS07170 at 231.280 kb on + strand, within EX31_RS07170 at 231.281 kb on - strand, within EX31_RS07170 at 231.342 kb on + strand, within EX31_RS07170 at 231.345 kb on + strand, within EX31_RS07170 at 231.345 kb on + strand, within EX31_RS07170 at 231.427 kb on - strand, within EX31_RS07170 at 231.437 kb on - strand, within EX31_RS07170 at 231.819 kb on + strand at 232.005 kb on + strand at 232.006 kb on - strand at 232.029 kb on + strand at 232.030 kb on - strand at 232.066 kb on - strand at 232.223 kb on - strand, within EX31_RS07175 at 232.223 kb on - strand, within EX31_RS07175 at 232.223 kb on - strand, within EX31_RS07175 at 232.255 kb on - strand, within EX31_RS07175 at 232.255 kb on - strand, within EX31_RS07175 at 232.255 kb on - strand, within EX31_RS07175 at 232.262 kb on - strand, within EX31_RS07175 at 232.266 kb on + strand, within EX31_RS07175 at 232.420 kb on - strand, within EX31_RS07175 at 232.420 kb on - strand, within EX31_RS07175 at 232.443 kb on - strand, within EX31_RS07175 at 232.443 kb on - strand, within EX31_RS07175 at 232.471 kb on - strand, within EX31_RS07175 at 232.491 kb on - strand, within EX31_RS07175 at 232.500 kb on + strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.501 kb on - strand, within EX31_RS07175 at 232.556 kb on - strand, within EX31_RS07175 at 232.566 kb on - strand, within EX31_RS07175 at 232.669 kb on + strand, within EX31_RS07175 at 232.669 kb on + strand, within EX31_RS07175 at 232.670 kb on - strand, within EX31_RS07175 at 232.768 kb on - strand, within EX31_RS07175 at 232.768 kb on - strand, within EX31_RS07175 at 232.803 kb on + strand, within EX31_RS07175 at 232.804 kb on - strand, within EX31_RS07175 at 232.804 kb on - strand, within EX31_RS07175 at 232.832 kb on - strand, within EX31_RS07175 at 232.903 kb on - strand, within EX31_RS07175 at 232.949 kb on - strand at 232.968 kb on - strand at 232.968 kb on - strand at 232.997 kb on - strand at 232.997 kb on - strand at 232.997 kb on - strand at 233.008 kb on - strand at 233.033 kb on - strand at 233.033 kb on - strand at 233.033 kb on - strand at 233.069 kb on - strand at 233.093 kb on + strand at 233.093 kb on + strand at 233.093 kb on + strand at 233.093 kb on + strand at 233.212 kb on - strand, within EX31_RS07180 at 233.294 kb on - strand, within EX31_RS07180 at 233.296 kb on - strand, within EX31_RS07180 at 233.498 kb on + strand, within EX31_RS07180 at 233.499 kb on - strand, within EX31_RS07180 at 233.499 kb on - strand, within EX31_RS07180 at 233.515 kb on - strand, within EX31_RS07180 at 233.577 kb on - strand, within EX31_RS07180 at 233.632 kb on - strand, within EX31_RS07180 at 233.664 kb on - strand at 233.664 kb on - strand at 233.714 kb on - strand at 233.842 kb on + strand at 233.843 kb on - strand at 233.844 kb on + strand at 233.845 kb on - strand at 233.848 kb on + strand at 233.848 kb on + strand at 233.848 kb on + strand at 233.848 kb on + strand at 233.848 kb on + strand at 233.848 kb on + strand at 233.849 kb on - strand at 233.849 kb on - strand at 233.849 kb on - strand at 233.849 kb on - strand at 233.849 kb on - strand at 233.849 kb on - strand at 233.849 kb on - strand at 233.849 kb on - strand at 233.850 kb on + strand at 233.850 kb on + strand at 233.850 kb on + strand at 233.850 kb on + strand at 233.850 kb on + strand at 233.850 kb on + strand at 233.851 kb on - strand at 233.851 kb on - strand at 233.851 kb on - strand at 233.854 kb on + strand at 233.854 kb on + strand at 233.855 kb on - strand at 233.855 kb on - strand at 233.855 kb on - strand at 233.893 kb on - strand at 233.949 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 231,148 + EX31_RS07170 0.65 +0.2 231,208 + EX31_RS07170 0.68 -1.1 231,208 + EX31_RS07170 0.68 +0.1 231,280 + EX31_RS07170 0.71 -0.1 231,280 + EX31_RS07170 0.71 -0.4 231,281 - EX31_RS07170 0.71 +1.9 231,342 + EX31_RS07170 0.74 +1.6 231,345 + EX31_RS07170 0.74 +0.1 231,345 + EX31_RS07170 0.74 +0.4 231,427 - EX31_RS07170 0.78 +1.5 231,437 - EX31_RS07170 0.78 +1.3 231,819 + +0.9 232,005 + -0.4 232,006 - +0.3 232,029 + +0.3 232,030 - +0.6 232,066 - +1.2 232,223 - EX31_RS07175 0.23 -1.0 232,223 - EX31_RS07175 0.23 +0.2 232,223 - EX31_RS07175 0.23 -0.6 232,255 - EX31_RS07175 0.26 +0.3 232,255 - EX31_RS07175 0.26 +1.2 232,255 - EX31_RS07175 0.26 +0.8 232,262 - EX31_RS07175 0.27 +0.4 232,266 + EX31_RS07175 0.27 -0.4 232,420 - EX31_RS07175 0.42 -1.9 232,420 - EX31_RS07175 0.42 +0.5 232,443 - EX31_RS07175 0.44 -0.4 232,443 - EX31_RS07175 0.44 +2.3 232,471 - EX31_RS07175 0.47 -3.1 232,491 - EX31_RS07175 0.49 -1.7 232,500 + EX31_RS07175 0.49 -0.9 232,501 - EX31_RS07175 0.49 -0.1 232,501 - EX31_RS07175 0.49 -1.3 232,501 - EX31_RS07175 0.49 +1.9 232,501 - EX31_RS07175 0.49 +0.5 232,501 - EX31_RS07175 0.49 -1.1 232,501 - EX31_RS07175 0.49 -1.6 232,501 - EX31_RS07175 0.49 -0.9 232,501 - EX31_RS07175 0.49 -0.1 232,501 - EX31_RS07175 0.49 -0.8 232,501 - EX31_RS07175 0.49 -1.2 232,501 - EX31_RS07175 0.49 -2.4 232,501 - EX31_RS07175 0.49 -0.8 232,501 - EX31_RS07175 0.49 -0.9 232,501 - EX31_RS07175 0.49 -0.7 232,501 - EX31_RS07175 0.49 -0.8 232,501 - EX31_RS07175 0.49 +1.9 232,501 - EX31_RS07175 0.49 -1.8 232,556 - EX31_RS07175 0.55 -1.9 232,566 - EX31_RS07175 0.56 -0.1 232,669 + EX31_RS07175 0.66 +0.6 232,669 + EX31_RS07175 0.66 +0.6 232,670 - EX31_RS07175 0.66 -1.3 232,768 - EX31_RS07175 0.75 -0.1 232,768 - EX31_RS07175 0.75 -1.3 232,803 + EX31_RS07175 0.78 -1.4 232,804 - EX31_RS07175 0.79 +0.3 232,804 - EX31_RS07175 0.79 -1.7 232,832 - EX31_RS07175 0.81 -0.9 232,903 - EX31_RS07175 0.88 -0.2 232,949 - +1.8 232,968 - -0.7 232,968 - -0.4 232,997 - -0.2 232,997 - -3.0 232,997 - -0.1 233,008 - +1.0 233,033 - +0.4 233,033 - +1.2 233,033 - -0.0 233,069 - +0.2 233,093 + +0.9 233,093 + +0.9 233,093 + +1.9 233,093 + +0.6 233,212 - EX31_RS07180 0.13 +0.2 233,294 - EX31_RS07180 0.27 +0.9 233,296 - EX31_RS07180 0.27 -0.2 233,498 + EX31_RS07180 0.63 -0.1 233,499 - EX31_RS07180 0.63 -0.2 233,499 - EX31_RS07180 0.63 +0.8 233,515 - EX31_RS07180 0.66 -0.1 233,577 - EX31_RS07180 0.77 +1.9 233,632 - EX31_RS07180 0.87 -0.9 233,664 - +0.3 233,664 - +2.5 233,714 - +2.5 233,842 + +0.9 233,843 - +0.7 233,844 + +0.9 233,845 - +0.7 233,848 + -1.1 233,848 + +0.2 233,848 + -0.4 233,848 + -0.4 233,848 + -0.8 233,848 + -1.6 233,849 - +0.9 233,849 - +0.1 233,849 - -0.1 233,849 - -1.1 233,849 - -1.6 233,849 - -1.3 233,849 - +0.3 233,849 - -1.6 233,850 + -0.4 233,850 + -0.8 233,850 + +2.1 233,850 + -0.6 233,850 + +0.4 233,850 + +1.3 233,851 - +1.5 233,851 - +0.1 233,851 - -0.3 233,854 + +0.3 233,854 + -1.1 233,855 - -1.1 233,855 - +1.1 233,855 - -0.4 233,893 - +0.9 233,949 - +1.5
Or see this region's nucleotide sequence