Experiment: Ammonium chloride (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS07050 and EX31_RS07055 overlap by 4 nucleotides EX31_RS07055 and EX31_RS07060 are separated by 26 nucleotides EX31_RS07060 and EX31_RS07065 are separated by 0 nucleotides
EX31_RS07050: EX31_RS07050 - sugar ABC transporter permease, at 203,392 to 204,294
_RS07050
EX31_RS07055: EX31_RS07055 - carbohydrate ABC transporter permease, at 204,291 to 205,130
_RS07055
EX31_RS07060: EX31_RS07060 - carbohydrate kinase family protein, at 205,157 to 206,110
_RS07060
EX31_RS07065: EX31_RS07065 - sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC, at 206,111 to 207,205
_RS07065
Position (kb) 205
206
207 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 204.176 kb on - strand, within EX31_RS07050 at 204.247 kb on + strand at 204.247 kb on + strand at 204.247 kb on + strand at 204.247 kb on + strand at 204.248 kb on - strand at 204.260 kb on + strand at 204.261 kb on - strand at 204.261 kb on - strand at 204.261 kb on - strand at 204.261 kb on - strand at 204.317 kb on - strand at 204.459 kb on - strand, within EX31_RS07055 at 204.476 kb on - strand, within EX31_RS07055 at 204.476 kb on - strand, within EX31_RS07055 at 204.584 kb on + strand, within EX31_RS07055 at 204.584 kb on + strand, within EX31_RS07055 at 204.639 kb on + strand, within EX31_RS07055 at 204.675 kb on + strand, within EX31_RS07055 at 204.676 kb on - strand, within EX31_RS07055 at 204.733 kb on - strand, within EX31_RS07055 at 204.733 kb on - strand, within EX31_RS07055 at 204.844 kb on + strand, within EX31_RS07055 at 204.845 kb on - strand, within EX31_RS07055 at 205.190 kb on + strand at 205.191 kb on - strand at 205.191 kb on - strand at 205.396 kb on + strand, within EX31_RS07060 at 205.396 kb on + strand, within EX31_RS07060 at 205.396 kb on + strand, within EX31_RS07060 at 205.396 kb on + strand, within EX31_RS07060 at 205.396 kb on + strand, within EX31_RS07060 at 205.397 kb on - strand, within EX31_RS07060 at 205.397 kb on - strand, within EX31_RS07060 at 205.507 kb on + strand, within EX31_RS07060 at 205.507 kb on + strand, within EX31_RS07060 at 205.507 kb on + strand, within EX31_RS07060 at 205.507 kb on + strand, within EX31_RS07060 at 205.507 kb on + strand, within EX31_RS07060 at 205.508 kb on - strand, within EX31_RS07060 at 205.610 kb on + strand, within EX31_RS07060 at 205.789 kb on + strand, within EX31_RS07060 at 205.988 kb on + strand, within EX31_RS07060 at 206.131 kb on - strand at 206.350 kb on - strand, within EX31_RS07065 at 206.360 kb on + strand, within EX31_RS07065 at 206.380 kb on + strand, within EX31_RS07065 at 206.488 kb on - strand, within EX31_RS07065 at 206.488 kb on - strand, within EX31_RS07065 at 206.613 kb on + strand, within EX31_RS07065 at 206.613 kb on + strand, within EX31_RS07065 at 206.613 kb on + strand, within EX31_RS07065 at 206.614 kb on - strand, within EX31_RS07065 at 206.617 kb on + strand, within EX31_RS07065 at 206.673 kb on + strand, within EX31_RS07065 at 206.673 kb on + strand, within EX31_RS07065 at 206.673 kb on + strand, within EX31_RS07065 at 206.673 kb on + strand, within EX31_RS07065 at 206.673 kb on + strand, within EX31_RS07065 at 206.674 kb on - strand, within EX31_RS07065 at 206.675 kb on + strand, within EX31_RS07065 at 206.675 kb on + strand, within EX31_RS07065 at 206.675 kb on + strand, within EX31_RS07065 at 206.675 kb on + strand, within EX31_RS07065 at 206.675 kb on + strand, within EX31_RS07065 at 206.675 kb on + strand, within EX31_RS07065 at 206.675 kb on + strand, within EX31_RS07065 at 206.675 kb on + strand, within EX31_RS07065 at 206.675 kb on + strand, within EX31_RS07065 at 206.675 kb on + strand, within EX31_RS07065 at 206.675 kb on + strand, within EX31_RS07065 at 206.676 kb on - strand, within EX31_RS07065 at 206.676 kb on - strand, within EX31_RS07065 at 206.676 kb on - strand, within EX31_RS07065 at 206.676 kb on - strand, within EX31_RS07065 at 206.676 kb on - strand, within EX31_RS07065 at 206.716 kb on + strand, within EX31_RS07065 at 206.743 kb on + strand, within EX31_RS07065 at 206.743 kb on + strand, within EX31_RS07065 at 206.748 kb on + strand, within EX31_RS07065 at 206.748 kb on + strand, within EX31_RS07065 at 206.792 kb on - strand, within EX31_RS07065 at 206.854 kb on + strand, within EX31_RS07065 at 206.882 kb on - strand, within EX31_RS07065 at 206.977 kb on - strand, within EX31_RS07065
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; no shaking remove 204,176 - EX31_RS07050 0.87 -0.9 204,247 + +0.2 204,247 + +2.3 204,247 + -0.6 204,247 + +1.9 204,248 - +1.0 204,260 + +1.9 204,261 - -0.4 204,261 - +1.6 204,261 - +2.1 204,261 - +0.9 204,317 - +1.5 204,459 - EX31_RS07055 0.20 +1.2 204,476 - EX31_RS07055 0.22 -0.4 204,476 - EX31_RS07055 0.22 -0.4 204,584 + EX31_RS07055 0.35 +1.0 204,584 + EX31_RS07055 0.35 -0.6 204,639 + EX31_RS07055 0.41 -0.1 204,675 + EX31_RS07055 0.46 -0.3 204,676 - EX31_RS07055 0.46 +1.2 204,733 - EX31_RS07055 0.53 -0.4 204,733 - EX31_RS07055 0.53 +1.8 204,844 + EX31_RS07055 0.66 +0.7 204,845 - EX31_RS07055 0.66 +0.8 205,190 + -0.6 205,191 - +0.8 205,191 - +0.9 205,396 + EX31_RS07060 0.25 +1.1 205,396 + EX31_RS07060 0.25 +1.3 205,396 + EX31_RS07060 0.25 +2.6 205,396 + EX31_RS07060 0.25 -0.7 205,396 + EX31_RS07060 0.25 -0.3 205,397 - EX31_RS07060 0.25 +0.9 205,397 - EX31_RS07060 0.25 -2.2 205,507 + EX31_RS07060 0.37 -1.9 205,507 + EX31_RS07060 0.37 +0.2 205,507 + EX31_RS07060 0.37 -0.7 205,507 + EX31_RS07060 0.37 +1.5 205,507 + EX31_RS07060 0.37 +0.9 205,508 - EX31_RS07060 0.37 +0.1 205,610 + EX31_RS07060 0.47 +1.3 205,789 + EX31_RS07060 0.66 +0.0 205,988 + EX31_RS07060 0.87 +2.6 206,131 - -0.1 206,350 - EX31_RS07065 0.22 +0.6 206,360 + EX31_RS07065 0.23 +0.5 206,380 + EX31_RS07065 0.25 +1.2 206,488 - EX31_RS07065 0.34 -1.1 206,488 - EX31_RS07065 0.34 +1.9 206,613 + EX31_RS07065 0.46 -0.1 206,613 + EX31_RS07065 0.46 +1.3 206,613 + EX31_RS07065 0.46 -1.7 206,614 - EX31_RS07065 0.46 -1.4 206,617 + EX31_RS07065 0.46 +0.6 206,673 + EX31_RS07065 0.51 -0.7 206,673 + EX31_RS07065 0.51 -0.7 206,673 + EX31_RS07065 0.51 -0.6 206,673 + EX31_RS07065 0.51 +1.5 206,673 + EX31_RS07065 0.51 -0.9 206,674 - EX31_RS07065 0.51 -0.7 206,675 + EX31_RS07065 0.52 -0.4 206,675 + EX31_RS07065 0.52 +1.6 206,675 + EX31_RS07065 0.52 -1.3 206,675 + EX31_RS07065 0.52 +0.1 206,675 + EX31_RS07065 0.52 -0.7 206,675 + EX31_RS07065 0.52 -0.1 206,675 + EX31_RS07065 0.52 -1.1 206,675 + EX31_RS07065 0.52 -1.3 206,675 + EX31_RS07065 0.52 +1.5 206,675 + EX31_RS07065 0.52 +0.2 206,675 + EX31_RS07065 0.52 -1.3 206,676 - EX31_RS07065 0.52 +1.0 206,676 - EX31_RS07065 0.52 -1.2 206,676 - EX31_RS07065 0.52 +0.9 206,676 - EX31_RS07065 0.52 -0.4 206,676 - EX31_RS07065 0.52 +0.7 206,716 + EX31_RS07065 0.55 -1.4 206,743 + EX31_RS07065 0.58 -0.3 206,743 + EX31_RS07065 0.58 -0.0 206,748 + EX31_RS07065 0.58 +0.2 206,748 + EX31_RS07065 0.58 +0.4 206,792 - EX31_RS07065 0.62 +2.9 206,854 + EX31_RS07065 0.68 -0.1 206,882 - EX31_RS07065 0.70 +0.6 206,977 - EX31_RS07065 0.79 +0.5
Or see this region's nucleotide sequence